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  • bEnd.3 [BEND3] (小鼠脑微血管内皮细胞) (STR鉴定正确)
  • bEnd.3 [BEND3] (小鼠脑微血管内皮细胞) (STR鉴定正确)
  • bEnd.3 [BEND3] (小鼠脑微血管内皮细胞) (STR鉴定正确)
  • bEnd.3 [BEND3] (小鼠脑微血管内皮细胞) (STR鉴定正确)
  • bEnd.3 [BEND3] (小鼠脑微血管内皮细胞) (STR鉴定正确)
  • bEnd.3 [BEND3] (小鼠脑微血管内皮细胞) (STR鉴定正确)
  • bEnd.3 [BEND3] (小鼠脑微血管内皮细胞) (STR鉴定正确)

bEnd.3 [BEND3] (小鼠脑微血管内皮细胞) (STR鉴定正确)

货号:CL-0598

规格:1×10⁶cells/T25培养瓶

价格: ¥1800

生长培养基:DMEM10% FBS1% P/S

数量: - +

  • bEnd.3 [BEND3] (小鼠脑微血管内皮细胞) (STR鉴定正确)
  • bEnd.3 [BEND3] (小鼠脑微血管内皮细胞) (STR鉴定正确)
  • bEnd.3 [BEND3] (小鼠脑微血管内皮细胞) (STR鉴定正确)
  • bEnd.3 [BEND3] (小鼠脑微血管内皮细胞) (STR鉴定正确)
  • bEnd.3 [BEND3] (小鼠脑微血管内皮细胞) (STR鉴定正确)
  • bEnd.3 [BEND3] (小鼠脑微血管内皮细胞) (STR鉴定正确)
  • bEnd.3 [BEND3] (小鼠脑微血管内皮细胞) (STR鉴定正确)

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bEnd.3 [BEND3] (小鼠脑微血管内皮细胞) (STR鉴定正确) ¥1800
bEnd.3 [BEND3]细胞专用培养基
  • ¥350
  • ¥450

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产品概述
产品信息
名称 bEnd.3 [BEND3] (小鼠脑微血管内皮细胞) (STR鉴定正确)
别称 bEND.3; b.End3; Bend.3; bEnd3; brain-derived Endothelial cells.3
种属 小鼠
生长特性 贴壁细胞
细胞形态 上皮细胞样
冻存条件 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮
培养方案A(默认)
生长培养基:
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
推荐传代比例 1:2-1:4
推荐换液频率 2-3次/周
注意事项 1.该细胞在低密度下形态不规则,细胞数量少,铺展面积大,高密度后形态会趋于一致,胞体密集;2.细胞增殖偏慢,初次传代建议1:2,细胞稳定后建议传代比例也不超过1:4;3.注意观察培养基颜色,颜色偏紫会导致细胞漂浮增加;4.消化时间会随着细胞生长时间和细胞密度而有所不同,需要结合消化时细胞状态来判断,消化到细胞可以滑落方可终止。
参考资料(来源文献)
背景描述 The cells were transformed by infection with the NTKmT retrovirus vector that expresses polyomavirus middle T antigen
年龄(性别) 6周龄
组织来源 大脑皮层
细胞类型 转化细胞系
生物安全等级 BSL-1
抗原表达情况 ICAM-1 +; VCAM-1 +; MAdCAM-1 +
基因表达情况 von Willebrand factor,ICAM-1 +; VCAM-1 +; MAdCAM-1 +,The endothelial nature of these cells was confirmed by the observed expression of von Willebrand factor and uptake of fluorescently labeled low density lipoprotein (LDL).,The expression of Peyer's Patch high endothelial receptor for lymphocytes, the mucosal vascular addressin (MAdCAM-1) and E-selectin can be induced on bEnd
保藏机构 ATCC; CRL-2299 BCRC; 60515 ECACC; 96091929
STR鉴定
  • STR位点信息
    18-3 18
    6-7 11,12
    5-5 14
    X-1 26
    1-2 13
    7-1 29
    8-1 16
    1-1 10
    19-2 13
    15-3 19.3,20.3
    12-1 17
    6-4 17
    4-2 18.3
    3-2 14
    2-1 9
    13-1

    16.2

    11-2 14
    17-2 12
  • STR鉴定图
参考文献
文献列表
  • A bone-targeted engineered exosome platform delivering siRNA to treat osteoporosis(2021/09/17)

    作者:Yongzhi Cui, Yuanyuan Guo, Li Kong

    期刊:Bioactive Materials

    DOI:10.1016/j.bioactmat.2021.09.015

    影响因子 :18.900

    引用产品:CL-0190 CL-0378 CL-0598

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    作者:Yong Xie, Yi Sun, Yudong Liu

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    DOI:10.1021/acsnano.3c04423

    影响因子 :17.100

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    作者:Huan Chen, Ting Li, Zhiyong Liu

    期刊:Nature Communications

    DOI:10.1038/s41467-022-35709-0

    影响因子 :16.600

    引用产品:CL-0238 CL-0598

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    作者:Xiuhua Yin, Hong Zhou, Mengling Zhang

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    DOI:10.1038/s41467-023-41489-y

    影响因子 :16.600

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    作者:Zhanchi Zhu, Yuanxin Zhai, Ying Hao

    期刊:Journal of Extracellular Vesicles

    DOI:10.1002/jev2.12255

    影响因子 :16.000

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    期刊:ACS Applied Materials & Interfaces

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    期刊:Applied Materials Today

    DOI:10.1016/j.apmt.2022.101453

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    引用产品:CL-0137 CL-0493 CL-0598

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    作者:Hongyan Pei, Rui Du, Zhongmei He

    期刊:INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

    DOI:10.1016/j.ijbiomac.2023.125815

    影响因子 :8.200

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    作者:Tingting Chen, Zimei Wu, Qiaodan Hou

    期刊:International Journal of Nanomedicine

    DOI:10.2147/IJN.S449290

    影响因子 :8.000

    引用产品:CL-0598

  • A Dual Receptor Targeting- and BBB Penetrating- Peptide Functionalized Polyethyleneimine Nanocomplex for Secretory Endostatin Gene Delivery to Malignant Glioma(2023/01/05)

    作者:Lu Lu, Hongyuan Chen, Longkun Wang

    期刊:International Journal of Nanomedicine

    DOI:10.2147/IJN.S270208

    影响因子 :8.000

    引用产品:CL-0598

  • The functional curcumin liposomes induce apoptosis in C6 glioblastoma cells and C6 glioblastoma stem cells in vitro and in animals(2017/02/17)

    作者:Yahua Wang, Xue Ying, Haolun Xu

    期刊:International Journal of Nanomedicine

    DOI:10.2147/IJN.S124276

    影响因子 :8.000

    引用产品:CL-0598

  • Biosynthetic Gas Vesicles Combined with Focused Ultrasound for Blood–Brain Barrier Opening(2022/12/28)

    作者:Jinghan Zhang, Fei Yan, Wei Zhang

    期刊:International Journal of Nanomedicine

    DOI:10.2147/IJN.S374039

    影响因子 :8.000

    引用产品:CL-0598

  • Active fraction of Polyrhachis vicina (Roger) alleviated cerebral ischemia/reperfusion injury by targeting SIRT3-mediated mitophagy and angiogenesis(2023/09/18)

    作者:Jie Wei, Jiaxiu Xie, Junhui He

    期刊:PHYTOMEDICINE

    DOI:10.1016/j.phymed.2023.155104

    影响因子 :7.900

    引用产品:CL-0598

  • Buyang Huanwu Decoction Promotes Angiogenesis after Cerebral Ischemia through Modulating Caveolin-1-Mediated Exosome MALAT1/YAP1/HIF-1α axis(2024/04/08)

    作者:Bowei Chen, Yaqian Xu, Fengming Tian

    期刊:PHYTOMEDICINE

    DOI:10.1016/j.phymed.2024.155609

    影响因子 :7.900

    引用产品:CL-0598

  • Elabela-APJ axis mediates angiogenesis via YAP/TAZ pathway in cerebral ischemia/reperfusion injury(2023/02/21)

    作者:Wenyu Li, Pengfei Xu, Lingqi Kong

    期刊:Translational Research

    DOI:10.1016/j.trsl.2023.02.002

    影响因子 :7.800

    引用产品:CL-0598

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    作者:Bowen Zhao, Yueqi Ni, Hong Zhang

    期刊:Cell Death Discovery

    DOI:10.1038/s41420-022-01222-y

    影响因子 :7.000

    引用产品:CL-0598 CM-0598 CM-H130 CP-H130

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    作者:Lin Zhao, Hongyuan Chen, Lu Lu

    期刊:Life Sciences

    DOI:10.1016/j.lfs.2021.119113

    影响因子 :6.100

    引用产品:CL-0598

  • Exosome-mediated delivery platform of biomacromolecules into the brain: Cetuximab in combination with doxorubicin for glioblastoma therapy(2024/05/28)

    作者:Liuxiang Chu, Aiping Wang, Yuchen Sun

    期刊:INTERNATIONAL JOURNAL OF PHARMACEUTICS

    DOI:10.1016/j.ijpharm.2024.124262

    影响因子 :5.800

    引用产品:CL-0598

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    作者:Liping Zhai, Hongyan Pei, Heping Shen

    期刊:INTERNATIONAL IMMUNOPHARMACOLOGY

    DOI:10.1016/j.intimp.2023.109815

    影响因子 :5.600

    引用产品:CL-0493 CL-0598

  • Minimally invasive vagus nerve stimulation modulates mast cell degranulation via the microbiota-gut-brain axis to ameliorate blood-brain barrier and intestinal barrier damage following ischemic stroke(2024/04/10)

    作者:Yanan Wang, Qianqian Tan, Mingdong Pan

    期刊:INTERNATIONAL IMMUNOPHARMACOLOGY

    DOI:10.1016/j.intimp.2024.112030

    影响因子 :5.600

    引用产品:CL-0598

  • Mertk Reduces Blood-Spinal Cord Barrier Permeability Through the Rhoa/Rock1/P-MLC Pathway After Spinal Cord Injury(2024/04/09)

    作者:Jiezhao Lin, Yuanfang Sun, Bin Xia

    期刊:Neuroscience Bulletin

    DOI:10.1007/s12264-024-01199-x

    影响因子 :5.600

    引用产品:CL-0598

  • MCC950 alleviates seizure severity and angiogenesis by inhibiting NLRP3/ IL-1β signaling pathway-mediated pyroptosis in mouse model of epilepsy(2023/11/30)

    作者:Yongri Hong, Caichuan Wei, Miaoying Fu

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    DOI:10.1016/j.intimp.2023.111236

    影响因子 :5.600

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  • Knockdown of Smox protects the integrity of the blood-brain barrier through antioxidant effect and Nrf2 pathway activation in stroke(2023/11/18)

    作者:Guangtian Wang, Zhihui Li, Peng Lin

    期刊:INTERNATIONAL IMMUNOPHARMACOLOGY

    DOI:10.1016/j.intimp.2023.111183

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  • USP14 inhibition promotes recovery by protecting BBB integrity and attenuating neuroinflammation in MCAO mice(2023/06/02)

    作者:Wenzhong Hou, Jianping Yao, Junjie Liu

    期刊:CNS Neuroscience & Therapeutics

    DOI:10.1111/cns.14292

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    作者:Chengjie Wu, Yalan Pan, Lining Wang

    期刊:CNS Neuroscience & Therapeutics

    DOI:10.1111/cns.14439

    影响因子 :5.500

    引用产品:CL-0190 CL-0598

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    DOI:10.1016/j.cbi.2023.110807

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    作者:Zhiyi Liu, Wei Hua, Sinan Jin

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    作者:Yiqian Xu, Xingyue Fang, Zhenqiang Zhao

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    DOI:10.1016/j.ejphar.2023.176228

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    DOI:10.1002/2211-5463.13067

    影响因子 :2.600

    引用产品:CL-0598

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何帆

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【南京】

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