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产品概述
产品信息
别名 | CSF-1, MGI-IM |
物种 | Mouse |
表达宿主 | E.coli |
序列信息 | MKEVSEHCSHMIGNGHLKVLQQLIDSQMETSCQIAFEFVDQEQLDDPVCYLKKAFFLVQDIIDETMRFKDNTPNANATERLQELSNNLNSCFTKDYEEQNKACVRTFHETPLQLLEKIKNFFNETKNLLEKDWNIFTKNCNNSFAKCSSRDVVTKP withpolyhistidine tag at the C-terminus |
检索号 | Q9QYH9.1 |
分子量 | 19.02 kDa |
表观分子量 | 17-25 kDa |
标签 | His-tag at the C-terminus |
生物活性 | Measure by its ability to induce proliferation in NFS-60 cells.The ED50 for this effect is <2 ng/mL.The specific activity of recombinant mouse M-CSF is approximately >5 x 10^5 IU/mg. |
产品特性
纯度 | >98% as determined by SDS-PAGE. Ni-NTA chromatography |
内毒素 | <0.1 EU per 1 μg of the protein by the LAL method. |
保存 | Lyophilized protein should be stored at -5~-20°C for 1 year. Upon reconstitution, store at 2-8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g.0.1%BSA,10%FBS,5%HSA or 5% trehalose solution), protein aliquots should be stored at -5~-20°C or -80°C for 3-6 months. |
运输 | Ambient temperature or ice pack. |
制剂 | The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 8.0. |
复融 | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL.Do Not Vortex! Vigorous shaking may impair the biological activity of the protein. |
背景介绍
参考文献
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A self-assembled bilayer polypeptide-engineered hydrogel for spatiotemporal modulation of bactericidal and anti-inflammation process in osteomyelitis treatment (2022-09-15)
期刊:JOURNAL OF NANOBIOTECHNOLOGY
DOI:10.1186/s12951-022-01614-3
影响因子 :9.4
引用产品: RPMI-1640 培养基 , M-CSF/CSF-1/MGI-IM, Human, Recombinant
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