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产品概述
产品信息
| 别名 | Ifa, Ifa8 |
| 物种 | Mouse |
| 表达宿主 | E.coli |
| 序列信息 | CDLPQTHNLRNKRALTLLVQMRRLSPLSCLKDRKDFGFPQEKVDAQQIKKAQAIPVLSELTQQILNIFTSKDSSAAWNTTLLDSFCNDLHQQLNDLQGCLMQQVGVQEFPLTQEDALLAVRKYFHRITVYLREKKHSPCAWEVVRAEVWRALSSSANVLGRLREEK with polyhistidine tag at the N-terminus. |
| 检索号 | P01572.2 |
| 分子量 | 19.93 kDa |
| 表观分子量 | 17-25 kDa |
| 标签 | His-tag at the N-terminus |
| 生物活性 | Measure by its ability to protect L929 cells infected with encephalomyocarditis (EMC) virus.The ED50 for this effect is <10 pg/mL.The specific activity of recombinant mouse IFN alpha 1a is > 4 x 10^7 IU/mg. |
产品特性
| 纯度 | >98% as determined by SDS-PAGE. Ni-NTA chromatography |
| 内毒素 | <0.1 EU per 1 μg of the protein by the LAL method. |
| 保存 | Lyophilized protein should be stored at -5~-20°C for 1 year. Upon reconstitution, store at 2-8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g.0.1%BSA,10%FBS,5%HSA or 5% trehalose solution), protein aliquots should be stored at -5~-20°C or -80°C for 3-6 months. |
| 运输 | Ambient temperature or ice pack. |
| 制剂 | The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 7.4. |
| 复融 | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL.Do Not Vortex! Vigorous shaking may impair the biological activity of the protein. |
背景介绍
Interferon alpha-1 or interferon alpha-D, encoded by the human IFNA1gene, is a member of the type I interferon family . It is expressed in peripheral blood leukocytes and lymphoblastoid cells. There are four highly conserved cysteine residues which form two disulfide bonds. One bond is a necessity for biological activity, which plays an critical role in conducting non-specific resistance against a variety of viral infections, and regulation of cell proliferation and modulates immune responses.
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