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产品概述
产品信息
| 别名 | TNFSF2, Cachectin, Differentiation-inducing factor (DIF), Necrosin, Cytotoxin, TNS_x0002_F1A |
| 物种 | Human |
| 表达宿主 | E.coli |
| 序列信息 | MVRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL with polyhistidine tag at the C-terminus. |
| 检索号 | P01375.1 |
| 分子量 | 18.3 kDa |
| 表观分子量 | 17 kDa |
| 标签 | His-tag at the C-terminus |
| 生物活性 | Measure by its ability to induce cytotoxicity in L929 cells in the presence of actinomycin D.The ED50 for this effect is <0.2 pg/mL.The specific activity of recombinant human TNF alpha is approximately >9 x 10^9 IU/mg. |
产品特性
| 纯度 | >98% as determined by SDS-PAGE. Ni-NTA chromatography |
| 内毒素 | <0.1 EU per 1 μg of the protein by the LAL method. |
| 保存 | Lyophilized protein should be stored at -5~-20°C for 1 year. Upon reconstitution, store at 2-8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g.0.1% BSA,10%FBS,5%HSA or 5% trehalose solution), protein aliquots should be stored at -5~-20°C or -80°C for 3-6 months. |
| 运输 | Ambient temperature or ice pack. |
| 制剂 | The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 8.0. |
| 复融 | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL.Do Not Vortex! Vigorous shaking may impair the biological activity of the protein. |
背景介绍
参考文献
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A microfluidic platform integrating dynamic cell culture and dielectrophoretic manipulation for in situ assessment of endothelial cell mechanics (2023-06-30)
作者：Hao Yang:1.Robotics and Microsystems Center, College of Mechanical and Electrical Engineering, Soochow University, Suzhou 215000, China. yhao@suda.edu.cn. ; Tao Chen:1.Robotics and Microsystems Center, College of Mechanical and Electrical Engineering, Soochow University, Suzhou 215000, China. yhao@suda.edu.cn. ; Yichong Hu:1.Robotics and Microsystems Center, College of Mechanical and Electrical Engineering, Soochow University, Suzhou 215000, China. yhao@suda.edu.cn. ; Fuzhou Niu:1.School of Mechanical Engineering, Suzhou University of Science and Technology, Suzhou 215000, China. ; Xinyu Zheng:1.Suzhou Medical College of Soochow University, Suzhou, Jiangsu, China. ; Haizhen Sun:1.Robotics and Microsystems Center, College of Mechanical and Electrical Engineering, Soochow University, Suzhou 215000, China. yhao@suda.edu.cn. ; Liang Cheng:1.Institute of Functional Nano & Soft Materials (FUNSOM), Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University
期刊：A microfluidic platform integrating dynamic cell culture and dielectrophoretic manipulation for in situ assessment of endothelial cell mechanics
影响因子 ：6.1
引用产品： 人脐静脉内皮细胞 , TNF-α/ TNFA/ TNFSF2, Human, Recombinant , Calcein-AM/PI活死细胞双染试剂盒
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