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名称 | 9L/lacZ (大鼠胶质肉瘤细胞) (种属鉴定正确) |
别称 | 9L/LacZ |
种属 | 大鼠 |
生长特性 | 贴壁细胞 |
细胞形态 | 成纤维细胞样 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养方案A(默认) |
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
|
推荐传代比例 | 1:4-1:8 |
推荐换液频率 | 2-3次/周 |
参考资料(来源文献)
背景描述 | The cells constitutively express the lacZ reporter gene product, E. coli derived beta-gal, as revealed on tissue sections by histochemical stain, and single tumor cells can be identified. Lymphocytes and other responding cells can be identified by double labeling with antibodies on the same slide. The contrast between stained cells and background facilitates image analysis. The beta-gal expression is very stable, but cells may need to be re-cloned after months of growth in culture. |
年龄(性别) | 雄性 |
组织来源 | 脑,胶质细胞 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 胶质瘤细胞 |
生物安全等级 | BSL-1 |
致瘤性 | Yes, forms tumors in the brains of CD Fischer 344 rats |
基因表达情况 | beta galactosidase (beta-gal) |
保藏机构 | ATCC; CRL-2200 |
种属鉴定
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种属鉴定图
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