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| 名称 | SU-DHL-1 (人间变性大细胞淋巴瘤细胞) (STR鉴定正确) |
| 别称 | SU-DHL1; SUDHL1; SUDHL-1; SuDHL-1; SuDHL 1; Stanford University-Diffuse Histiocytic Lymphoma-1 |
| 种属 | 人源 |
| 生长特性 | 悬浮细胞 |
| 细胞形态 | 淋巴母细胞样 |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO 温度：液氮 |
| 培养方案A（默认） |
培养条件:
气相：空气，95%；CO2，5%, 温度：37℃
|
| 推荐传代比例 | 1:2-1:6 |
| 推荐换液频率 | 2-3次/周 |
参考资料(来源文献)
| 背景描述 | SU-DHL-1 is a histiocytic cell that was isolated from the lymph node of a White, 10-year-old, male patient with large cell lymphoma. The cells are surface Ig negative (sIG-). The cells are non-specific esterase, acid phosphatase and oil red O positive. The cells are periodic acid Schiff negative. These cells phagocytose Candida albicans and latex particles. The cells are reported to be very weakly E - rosette positive. ATCC confirmed this cell line is negative for the presence of Epstein-Barr viral DNA sequences via PCR. |
| 年龄（性别） | 男性；10岁 |
| 组织来源 | 淋巴结 |
| 细胞类型 | 肿瘤细胞 |
| 肿瘤类型 | 淋巴瘤细胞 |
| 生物安全等级 | BSL-1 |
| 倍增时间 | ~40-50 hours (DSMZ=ACC-356) |
| 受体表达情况 | CFS-1, expressed; (IL-1-R; IL-2-Rα; IL-6-R; TNFα-R; & c-fms), expressed; Surface Receptors: (Fc; IgMEAC; IgGEA; & E), not expressed |
| 抗原表达情况 | Monocyte Marker: CD163+ Lymphoid Marker: CD45- Progenitor Markers: CD10-, CD34- Activation Markers: CD30+, CD25+, CD70+, CD71+, CD80-, HLA-DR+, CD45- T-Cell Markers: CD2-, CD3-, CD4-, CD5+, CD7-, CD8- B-Cell Markers: CD19-, CD20-, CD21-, CD22- Myelom |
| 基因表达情况 | fusion gene NPM-ALK (p80)+; anaplastic lymphoma kinase (ALK)+; Ig not expressed |
| 保藏机构 | ATCC; CRL-2955 |
STR鉴定
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STR位点信息
Amelogenin X, Y CSF1PO 12 D2S1338 19,20 D3S1358 15,16 D5S818 11,12 D7S820 10,13 D8S1179 11 D13S317 9,13 D16S539 11 D18S51 15 D19S433 12,16 D21S11 29,30 FGA 21 PentaD 14 PentaE 11,15 TH01 6,7 TPOX 8 vWA 15,17 D6S1043 13 D12S391 17 D2S441 11,14 -
STR鉴定图
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