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产品概述
台盼蓝（Trypan Blue)或称台盼兰、锥虫蓝、曲利苯蓝，是细胞活性染料，常用于检测细胞膜的完整性和细胞的存活率。台盼蓝染色是组织和细胞培养中最常用的死细胞鉴定染色方法之一。丧失活性或细胞膜不完整的细胞可被台盼蓝染成蓝色而正常的活细胞细胞膜结构完整，能够排斥台盼蓝，细胞不会被染成蓝色。通常认为细胞膜完整性丧失，即可认为细胞已经死亡。而巨噬细胞能够吞噬台盼蓝，故可用于巨噬细胞的活体染色剂。另外需要注意的是，凋亡小体也有台盼蓝拒染现象。
台盼蓝染色后，通过显微镜下直接计数或显微镜下拍照后计数，就可以对细胞存活率进行比较精确的定量。染色只需3-5 min，操作简单。
产品信息
形态 | 液体 |
规格 | 100mL |
溶剂 | 0.85% NaCl溶液 |
台盼蓝 | 4 g/L |
储存条件 | 2-30℃ |
运输条件 | 常温 |
有效期 | 36个月 |
使用说明
1、收集细胞（贴壁细胞可能需要用胰酶和/或 EDTA 消化），1000 r/min 离心 5min，弃上清，制备单细胞悬液，并做适当稀释。
2、向细胞悬液中加入0.4%台盼蓝溶液，台盼蓝终浓度为0.04%，室温染色3 min（染色时间可以稍微延长，但不宜超过10 min） 。
3、吸取少量经过染色的细胞，用血球计数板计数，在显微镜下可以观察到，死细胞着蓝色并膨大，无光泽；活细胞则不着色并保持正常形态，有光泽。
4、计算活细胞存活率
细胞存活率（%）= 活细胞总数/（活细胞总数+死细胞总数）×100%
注意事项
1、染色时间不能太长，否则活细胞也会逐渐积累染料而染成颜色，使检测结果出现偏差；
2、细胞计数时，通常要比较精确地进行定量，每个细胞样品计数不少于 500 个细胞；
3、台盼蓝有潜在的致癌风险，操作时请穿实验服并戴一次性手套。
4、本产品长时间存放可能会出现少量颗粒状沉淀，可37℃水浴约10分钟以充分溶解沉淀，沉淀完全溶解后即可正常使用。
5、本产品仅用于科研或进一步研究使用，不用于诊断和治疗。
参考文献
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Administration of Arginine Vasotocin and modified Isotocin improve artificial propagation and post-spawning survival of female yellow catfish (2024-03-19)
作者：Yang Xiong: 1.Hubei Hongshan Laboratory, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China; Xu Wang: 1.Hubei Hongshan Laboratory, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China; Ruidong Sun: 1.Hubei Hongshan Laboratory, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China; Youbo Jiang: 1.Hubei Hongshan Laboratory, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China; Zhipeng He: 1.Hubei Hongshan Laboratory, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China; Jian Chen: 1.Fisheries Research Institute, Wuhan Academy of Agricultural Sciences, Wuhan 430207, China; Pei Li: 1.Fisheries Research Institute, Wuhan Academy of Agricultural Sciences, Wuhan 430207, China; Jie Mei: 1.Hubei Hongshan Laboratory, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China;
期刊：Administration of Arginine Vasotocin and modified Isotocin improve artificial propagation and post-spawning survival of female yellow catfish
DOI：10.1016/j.aquaculture.2024.740849
影响因子 ：4.5
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WISP2 downregulation inhibits the osteogenic differentiation of BMSCs in congenital scoliosis by regulating Wnt/β-catenin pathway (2023-06-09)
作者：Yang Zheng:1.Zhejiang Chinese Medical University, Hangzhou, China; Department of Orthopedics Surgery, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, China. ; Panyang Shen:1.Department of Orthopedics Surgery, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, China. ; Mengsha Tong:1.Zhejiang Chinese Medical University, Hangzhou, China. ; Hangchao Li:1.Zhejiang Chinese Medical University, Hangzhou, China. ; Conglin Ren:1.Zhejiang Chinese Medical University, Hangzhou, China. ; Fengqing Wu:1.Zhejiang Chinese Medical University, Hangzhou, China. ; Hanyu Li:1.Zhejiang Chinese Medical University, Hangzhou, China. ; Huan Yang:1.Department of Biochemistry, Zhejiang University School of Medicine, Hangzhou, China. ; Bingbing Cai:1.Department of Orthopedics, Xiaoshan Traditional Chinese Medical Hospital, Hangzhou, China. ; Weibin Du:1.Department of Orthopedics, Xiaoshan Traditional Chinese Medical Hospital, Hangzhou, China. ; Xing Z
期刊：WISP2 downregulation inhibits the osteogenic differentiation of BMSCs in congenital scoliosis by regulating Wnt/β-catenin pathway
DOI：10.1016/j.bbadis.2023.166783
影响因子 ：6.2
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Lactobacillus gasseri LGV03 isolated from the cervico-vagina of HPV-cleared women modulates epithelial innate immune responses and suppresses the growth of HPV-positive human cervical cancer cells (2023-06-16)
作者：Qiong Gao:1.Department of Gynecology, The Eighth Affiliated Hospital of Sun Yat-sen University, Shenzhen 518000, China. ; Tao Fan:1.Department of Obstetrics, The Eighth Affiliated Hospital of Sun Yat-sen University, Shenzhen 518003, China. ; Siying Luo:1.Department of Gynecology, The Eighth Affiliated Hospital of Sun Yat-sen University, Shenzhen 518000, China. ; Jieting Zheng:1.School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China. ; Lin Zhang:1.School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China. ; Longbing Cao:1.Department of Laboratory Medicine, The Seventh Affiliated Hospital of Southern Medical University, Foshan, Guangdong 528244, China. ; Zikang Zhang:1.Department of Laboratory Medicine, The Seventh Affiliated Hospital of Southern Medical University, Foshan, Guangdong 528244, China. ; Li Li:1.Department of Gynecology, University of Chinese Academy of Sciences Shenzhen Hospital (Guangming), She
期刊：Lactobacillus gasseri LGV03 isolated from the cervico-vagina of HPV-cleared women modulates epithelial innate immune responses and suppresses the growth of HPV-positive human cervical cancer cells
DOI：10.1016/j.tranon.2023.101714
影响因子 ：5.0
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TLR3 activation by Clonorchis sinensis infection alleviates the fluke-induced liver fibrosis (2023-05-11)
作者：Yuru Wang:1.State Key Laboratory for Zoonotic Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, and College of Veterinary Medicine, Jilin University, Changchun, China. ; Pengtao Gong:1.State Key Laboratory for Zoonotic Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, and College of Veterinary Medicine, Jilin University, Changchun, China. ; Xuancheng Zhang:1.State Key Laboratory for Zoonotic Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, and College of Veterinary Medicine, Jilin University, Changchun, China. ; Xiaocen Wang:1.State Key Laboratory for Zoonotic Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, and College of Veterinary Medicine, Jilin University, Changchun, China. ; Xu Zhang:1.State Key Laboratory for Zoonotic Diseases, Key Laboratory for Zoonosis Research of the Minis
期刊：TLR3 activation by Clonorchis sinensis infection alleviates the fluke-induced liver fibrosis
DOI：10.1371/journal.pntd.0011325
影响因子 ：3.8
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Single-cell RNA-seq reveals altered NK cell subsets and reduced levels of cytotoxic molecules in patients with ankylosing spondylitis (2001-02-20)
作者：Conglin Ren:1.The Third Clinical Medical College of Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China. ; Mingshuang Li:1.The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China. ; Yang Zheng:1.The Third Clinical Medical College of Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China. ; Bingbing Cai:1.Research Institute of Orthopedics, The Affiliated Jiangnan Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China. ; 2.Department of Orthopedics, Hangzhou Xiaoshan Hospital of Traditional Chinese Medicine, Hangzhou, Zhejiang, China. ; Weibin Du:1.Research Institute of Orthopedics, The Affiliated Jiangnan Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China. ; 2.Department of Orthopedics, Hangzhou Xiaoshan Hospital of Traditional Chinese Medicine, Hangzhou, Zhejiang, China. ; Helou Zhang:1.The Third Clinical Medical College of Zhejiang Chinese Medical University, Hangzhou, Zhejiang,
期刊：Single-cell RNA-seq reveals altered NK cell subsets and reduced levels of cytotoxic molecules in patients with ankylosing spondylitis
影响因子 ：5.3
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