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产品概述
RPMI-1640是Moore等人于1967年在美国纽约州法罗市的罗斯韦尔公园纪念研究所（Roswell Park Memorial Institute, RPMI）开发出来的，RPMI是该研究所开发的一类细胞培养基，1640是培养基代号。RPMI-1640是改进型的McCoy's 5A培养基，使用碳酸氢盐缓冲系统，与大多数哺乳动物细胞培养基不同的是其典型的PH8的配方。RPMI-1640培养基最初是为淋巴细胞培养专门设计的，现在已广泛应用于各种正常细胞和癌细胞的培养，尤其是悬浮细胞的培养，是使用最为广泛的培养基之一。不含葡萄糖的RPMI-1640培养基可以根据研究需要，随意调节葡萄糖的浓度，方便快捷。
本产品含有多类细胞培养所需的氨基酸、维生素、无机盐等多种成分，但不含蛋白质、脂类或任何生长因子，故此产品需搭配血清或无血清添加物使用。
成分说明
形态 | 液体 |
浓度 | 1× |
规格 | 500mL |
pH | 7.2-7.4 |
L-谷氨酰胺 | 2 mM |
NaHCO3 | 2000 mg/L |
D-葡萄糖 | 无 |
HEPES缓冲剂 | 无 |
酚红指示剂 | 5 mg/L |
储存条件 | 2-8℃，避光 |
运输条件 | 常温 |
有效期 | 18个月 |
注意事项
1、本产品经0.1μm过滤除菌，使用时应注意无菌操作，避免污染；
2、为保持本产品的最佳使用效果，请勿进行冻融处理；
3、本产品仅用于科研或进一步研究使用，不用于诊断和治疗。
参考文献
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Glucose restriction induces AMPK-SIRT1-mediated circadian clock gene Per expression and delays NSCLC progression (2023-09-30)
作者：Bohan Li:1.Department of Nutrition and Food Hygiene, Key Laboratory of Toxicology, School of Public Health, Nanjing Medical University, Nanjing, Jiangsu, China. ; Qianfeng Chen:1.Department of Nutrition and Food Hygiene, Key Laboratory of Toxicology, School of Public Health, Nanjing Medical University, Nanjing, Jiangsu, China. ; Yucong Feng:1.Department of Nutrition and Food Hygiene, Key Laboratory of Toxicology, School of Public Health, Nanjing Medical University, Nanjing, Jiangsu, China. ; Tao Wei:1.Department of Nutrition and Food Hygiene, Key Laboratory of Toxicology, School of Public Health, Nanjing Medical University, Nanjing, Jiangsu, China. ; Yuxia Zhong:1.Department of Nutrition and Food Hygiene, Key Laboratory of Toxicology, School of Public Health, Nanjing Medical University, Nanjing, Jiangsu, China. ; Yuandie Zhang:1.Department of Nutrition and Food Hygiene, Key Laboratory of Toxicology, School of Public Health, Nanjing Medical University, Nanjing, Jiangsu, China. ; Q
期刊：Glucose restriction induces AMPK-SIRT1-mediated circadian clock gene Per expression and delays NSCLC progression
DOI：10.1016/j.canlet.2023.216424
影响因子 ：9.7
引用产品： RPMI-1640 培养基 , RPMI-1640无糖 培养基
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1,25-Dihydroxyvitamin D3 Provides Benefits in Vitiligo Based on Modulation of CD8+ T Cell Glycolysis and Function (2023-11-06)
作者：Yujia Wei:1.Department of Dermatology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. ; Tingmei Wang:1.Department of Dermatology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. ; Xiaoqi Nie:1.Department of Dermatology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. ; Zeqi Shi:1.Department of Dermatology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. ; Zhong Liu:1.Department of Dermatology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. ; Ying Zeng:1.Department of Dermatology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. ; Ronghua Pan:1.Department of Dermatology, Tongji Hospital, Tongji Medical College, Huazhong
期刊：1,25-Dihydroxyvitamin D3 Provides Benefits in Vitiligo Based on Modulation of CD8+ T Cell Glycolysis and Function
影响因子 ：5.9
引用产品： RPMI-1640无糖 培养基
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Hyperglycemia Aggravates Periodontitis via Autophagy Impairment and ROS-Inflammasome-Mediated Macrophage Pyroptosis (2023-03-27)
作者：Zhenxing Zhao:1.College of Stomatology, Chongqing Medical University, Chongqing 401147, China. ; 2.Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing 401147, China. ; 3.Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing 401147, China. ; Ye Ming:1.College of Stomatology, Chongqing Medical University, Chongqing 401147, China. ; 2.Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing 401147, China. ; 3.Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing 401147, China. ; Xiang Li:1.College of Stomatology, Chongqing Medical University, Chongqing 401147, China. ; 2.Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing 401147, China. ; 3.Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing 401147, China. ; Hao Tan:1.College of Stomatology, Chongqing Medical University, Chongqing 401147,
期刊：Hyperglycemia Aggravates Periodontitis via Autophagy Impairment and ROS-Inflammasome-Mediated Macrophage Pyroptosis
影响因子 ：5.6
引用产品： RPMI-1640 培养基 , RPMI-1640无糖 培养基
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Type 2 diabetes affects arsenic metabolism via transporters in arsenic trioxide treated acute promyelocytic leukemia patients (2023-05-03)
作者：Mengliang Wu:1.Department of Pharmacy, First Affiliated Hospital of Harbin Medical University, 23 YouZheng Str, Nangang District, Harbin, China; Heilongjiang University of Chinese Medicine, 24 Heping Road, Xiangfang District, Harbin 150040, China. ; Chunrong Pang:1.Department of Pharmacy, First Affiliated Hospital of Harbin Medical University, 23 YouZheng Str, Nangang District, Harbin, China. ; Shengwen Lu:1.Heilongjiang University of Chinese Medicine, 24 Heping Road, Xiangfang District, Harbin 150040, China. ; Thomas H Hostetter:1.School of Medicine, University of North Carolina, Chapel Hill, NC, United States of America. ; Xin Hai:1.Department of Pharmacy, First Affiliated Hospital of Harbin Medical University, 23 YouZheng Str, Nangang District, Harbin, China. Electronic address: hai_xin@163.com. ;
期刊：Type 2 diabetes affects arsenic metabolism via transporters in arsenic trioxide treated acute promyelocytic leukemia patients
DOI：10.1016/j.etap.2023.104142
影响因子 ：4.3
引用产品： RPMI-1640无糖 培养基
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Neuroprotective effects of neural stem cells pretreated with neuregulin1β on PC12 cells exposed to oxygen-glucose deprivation/reoxygenation (2022-08-02)
作者：Qiu-Yue Zhai:1.Institute of Cerebrovascular Diseases, Medical Research Center, The Affiliated Hospital of Qingdao University, Taishan Scholars Construction Project Excellent Innovative Team of Shandong Province, Qingdao, Shandong Province, China. ; Yuan-Hua Ye:1.Department of Obstetrics, The Affiliated Hospital of Qingdao University, Qingdao, Shandong Province, China. ; Yu-Qian Ren:1.Institute of Cerebrovascular Diseases, Medical Research Center, The Affiliated Hospital of Qingdao University, Taishan Scholars Construction Project Excellent Innovative Team of Shandong Province, Qingdao, Shandong Province, China. ; Zhen-Hua Song:1.Institute of Cerebrovascular Diseases, Medical Research Center, The Affiliated Hospital of Qingdao University, Taishan Scholars Construction Project Excellent Innovative Team of Shandong Province, Qingdao, Shandong Province, China. ; Ke-Li Ge:1.Institute of Cerebrovascular Diseases, Medical Research Center, The Affiliated Hospital of Qingdao University, Tai
期刊：Neuroprotective effects of neural stem cells pretreated with neuregulin1β on PC12 cells exposed to oxygen-glucose deprivation/reoxygenation
影响因子 ：6.1
引用产品： RPMI-1640无糖 培养基
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Neuroprotective effects of methylcobalamin in cerebral ischemia/reperfusion injury through activation of the ERK1/2 signaling pathway (2008-01-20)
作者：Yunong Li:1.Department of Neurology, the Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China. ; Jiaolin Zheng:1.Department of Neurology, the Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China. ; Yanmei Zhu:1.Department of Neurology, the Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China. ; Youyang Qu:1.Department of Neurology, the Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China. ; Rui Suo:1.Department of Neurology, the Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China. ; Yulan Zhu:1.Department of Neurology, the Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China. Electronic address: z_yulan@outlook.com. ;
期刊：Neuroprotective effects of methylcobalamin in cerebral ischemia/reperfusion injury through activation of the ERK1/2 signaling pathway
DOI：10.1016/j.intimp.2021.108040
影响因子 ：5.6
引用产品： RPMI-1640无糖 培养基
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