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产品概述
M199全称Medium 199,即培养基199,是Morgan等在1950年设计的,最初用于鸡胚成纤维细胞的营养研究,现已广泛应用于各种动物细胞的培养,包括一些非哺乳类动物细胞。M199特别适用于非转化细胞的培养,也常用于病毒学、疫苗生产,以及大鼠胰腺上皮细胞和小鼠晶状体组织的培养。与其他基础培养基相比,M199含有独特的成分,包括腺嘌呤、腺苷、次黄嘌呤、胸腺嘧啶、以及其他的维生素。M199有两种平衡盐成分,Earle's盐成分常用于CO2环境,Hank's盐成分用于非CO2的环境。
本产品含有多类细胞培养所需的氨基酸、维生素、无机盐等多种成分,但不含蛋白质、脂类或任何生长因子,故此产品需搭配血清或无血清添加物使用。
成分说明
形态 | 液体 |
浓度 | 1× |
规格 | 500mL |
pH | 7.2-7.4 |
平衡盐 | Earle's 盐 |
L-谷氨酰胺 | 100 mg/L |
NaHCO3 | 2200 mg/L |
D-葡萄糖 | 1000 mg/L |
HEPES缓冲剂 | 无 |
酚红指示剂 | 20 mg/L |
储存条件 | 2-8℃,避光 |
运输条件 | 常温 |
有效期 | 18个月 |
注意事项
1、本产品经0.1 μm过滤除菌,使用时应注意无菌操作,避免污染;
2、为保持本产品的最佳使用效果,请勿进行冻融处理;
3、本产品仅用于科研或进一步研究使用,不用于诊断和治疗。
参考文献
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Effects of Chlorogenic Acid on In Vitro Maturation and Vitrification Cryopreservation of Sheep Oocytes (2025-01-16)
期刊:Veterinary Sciences
影响因子 :2.0
引用产品: M199 培养基
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Characterization and function of PTEN-induced putative kinase 1 (PINK1) in process of Zinc alleviates hepatic lipid deposition of yellow catfish (Pelteobagrus fulvidraco) (2024-03-26)
期刊:INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
DOI:10.1016/j.ijbiomac.2024.131156
影响因子 :8.2
引用产品: M199 培养基
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GFPT2: A novel biomarker in mesothelioma for diagnosis and prognosis and its molecular mechanism in malignant progression (2024-09-24)
期刊:BRITISH JOURNAL OF CANCER
DOI:10.1038/s41416-024-02830-4
影响因子 :6.4
引用产品: M199 培养基
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BDE-47-induced damage prevented by melatonin in grass carp hepatocytes (L8824) (2024-03-16)
期刊:ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
DOI:10.1007/s11356-024-32856-3
影响因子 :5.8
引用产品: M199 培养基
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Ammonia‐?nitrogen stress affects immune regulation via TNFα in yellow catfish (Pelteobagrus fulvidraco) (2024-01-18)
期刊:AQUACULTURE
DOI:10.1016/j.aquaculture.2024.740593
影响因子 :4.5
引用产品: M199 培养基
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The Nrf2/ARE pathway as a potential target to ameliorate atrazine-induced endocrine disruption in granulosa cells (2024-04-03)
期刊:POULTRY SCIENCE
影响因子 :4.4
引用产品: M199 培养基
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Mitochondrial damage and activation of NLRP3 induced by Yersinia ruckeri infection play an important role in the occurrence of diffuse acute inflammation of channel catfish (Ictalurus punctatus) (2024-04-12)
期刊:AQUACULTURE
DOI:10.1016/j.aquaculture.2024.740940
影响因子 :3.9
引用产品: M199 培养基
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PI3KR1 and AKT1 in largemouth bass (Micropterus salmoides): molecular cloning, characterization, and its involvement in the alleviation of hepatic glycogen deposition caused by insulin inclusion in vitro (2024-08-16)
期刊:FISH PHYSIOLOGY AND BIOCHEMISTRY
DOI:10.1007/s10695-024-01379-6
影响因子 :2.5
引用产品: M199 培养基 , Leibovitz's L-15 培养基
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Investigation of the Antiviral Mechanism of Curcumin Analog EF-24 against Siniperca?cachuatsi Rhabdovirus (2024-05-15)
期刊:Fishes
影响因子 :2.3
引用产品: M199 培养基
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Genetic and Pathogenic Characterization of an Iridovirus from the Cultured Largemouth Bass Micropterus salmoides (2024-08-08)
期刊:Fishes
影响因子 :2.1
引用产品: M199 培养基
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Salidroside Reduced Ca2+-CaM-CAMKII-Dependent eNOS/NO Activation to Decrease Endothelial Cell Injury Induced by Cold Combined with Hypoxia (2024-07-17)
期刊:CELL BIOCHEMISTRY AND BIOPHYSICS
DOI:10.1007/s12013-024-01434-2
影响因子 :1.8
引用产品: M199 培养基
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Effects of Leonurine on oocyte maturation and parthenogenetic embryo development in sheep (2024-03-05)
期刊:REPRODUCTION IN DOMESTIC ANIMALS
影响因子 :1.7
引用产品: M199 培养基
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Basic study on cryopreservation of rat calvarial osteoblasts with different cryoprotectants (2024-07-08)
期刊:CELL AND TISSUE BANKING
DOI:10.1007/s10561-024-10142-3
影响因子 :1.4
引用产品: M199 培养基
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SARM suppresses TRIF, TRAF3, and IRF3/7 mediated antiviral signaling in large yellow croaker Larimichthys crocea (2023-01-13)
期刊:Frontiers in Immunology
DOI:10.3389/fimmu.2022.1021443
影响因子 :7.3
引用产品: M199 培养基
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Monoclonal antibody targeting a novel linear epitope on nucleoprotein confers pan-reactivity to influenza A virus (2023-02-23)
期刊:APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
DOI:10.1007/s00253-023-12433-3
影响因子 :5.0
引用产品: DMEM高糖 培养基 , M199 培养基
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TBBPA and lead co-exposure induces grass carp liver cells apoptosis via ROS/JAK2/STAT3 signaling axis (2023-10-02)
期刊:FISH & SHELLFISH IMMUNOLOGY
影响因子 :4.7
引用产品: M199 培养基
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Effect of RNA interference with HIF-1α on the growth of pulmonary artery endothelial cells in broiler chickens: JXAU-LP-HIF-1α (2023-12-18)
期刊:POULTRY SCIENCE
影响因子 :4.4
引用产品: M199 培养基
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Teleost TRAF7, a protein functions in the host antiviral responses via NF-κB and IRF3/7 mediated signaling (2023-02-06)
期刊:Frontiers in Marine Science
DOI:10.3389/fmars.2023.1092732
影响因子 :3.7
引用产品: M199 培养基
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Putrescine alleviates the oxidative damage of cumulus-oocyte complex via improving fatty acid oxidation (2023-10-17)
期刊:BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
DOI:10.1016/j.bbrc.2023.149127
影响因子 :3.1
引用产品: M199 培养基
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Investigating the therapeutic effects and mechanisms of Roxadustat on peritoneal fibrosis Based on the TGF-β/Smad pathway (2023-12-14)
期刊:BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
DOI:10.1016/j.bbrc.2023.149387
影响因子 :3.1
引用产品: MET-5A 细胞 , MET-5A细胞专用培养基 , M199 培养基
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G-Quadruplex DNAzyme-Substrated CRISPR/Cas12 Assay for Label-Free Detection of Single-Celled Parasitic Infection (2022-10-07)
期刊:ACS Sensors
DOI:10.1021/acssensors.2c01104
影响因子 :9.6
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Melatonin relieves 2,2,4,4-tetrabromodiphenyl ether (BDE-47)-induced apoptosis and mitochondrial dysfunction through the AMPK-Sirt1-PGC-1α axis in fish kidney cells (CIK) (2022-02-02)
期刊:Ecotoxicology And Environmental Safety
DOI:10.1016/j.ecoenv.2022.113276
影响因子 :6.8
引用产品: M199 培养基
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Autophagy Delays Apoptotic Cell Death Induced by Siniperca chuatsi Rhabdovirus in Epithelioma Papulosum Cyprinid Cells (2021-08-06)
期刊:Viruses-Basel
影响因子 :5.0
引用产品: M199 培养基
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Small RNA sequencing reveals miRNAs important for hypoxic adaptation in the Tibetan chicken (2020-07-24)
期刊:British Poultry Science
DOI:10.1080/00071668.2020.1792835
影响因子 :2.0
引用产品: M199 培养基
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