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产品概述
M199全称Medium 199,即培养基199,是Morgan等在1950年设计的,最初用于鸡胚成纤维细胞的营养研究,现已广泛应用于各种动物细胞的培养,包括一些非哺乳类动物细胞。M199特别适用于非转化细胞的培养,也常用于病毒学、疫苗生产,以及大鼠胰腺上皮细胞和小鼠晶状体组织的培养。与其他基础培养基相比,M199含有独特的成分,包括腺嘌呤、腺苷、次黄嘌呤、胸腺嘧啶、以及其他的维生素。M199有两种平衡盐成分,Earle's盐成分常用于CO2环境,Hank's盐成分用于非CO2的环境。
本产品含有多类细胞培养所需的氨基酸、维生素、无机盐等多种成分,但不含蛋白质、脂类或任何生长因子,故此产品需搭配血清或无血清添加物使用。
成分说明
形态 | 液体 |
浓度 | 1× |
规格 | 500mL |
pH | 7.2-7.4 |
平衡盐 | Earle's 盐 |
L-谷氨酰胺 | 100 mg/L |
NaHCO3 | 2200 mg/L |
D-葡萄糖 | 1000 mg/L |
HEPES缓冲剂 | 无 |
酚红指示剂 | 20 mg/L |
储存条件 | 2-8℃,避光 |
运输条件 | 常温 |
有效期 | 18个月 |
注意事项
1、本产品经0.1 μm过滤除菌,使用时应注意无菌操作,避免污染;
2、为保持本产品的最佳使用效果,请勿进行冻融处理;
3、本产品仅用于科研或进一步研究使用,不用于诊断和治疗。
参考文献
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Characterization and function of PTEN-induced putative kinase 1 (PINK1) in process of Zinc alleviates hepatic lipid deposition of yellow catfish (Pelteobagrus fulvidraco) (2024-03-26)
作者:Lanfei Xiao:1.College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China; Nansha-South China Agricultural University Fishery Research Institute, Guangzhou 510642, China. ; Baojia Chen:1.Nansha-South China Agricultural University Fishery Research Institute, Guangzhou 510642, China. ; Chuan Chen:1.College of Marine Sciences, South China Agricultural University, Guangzhou 510642, China. ; Fei Xiao:1.College of Marine Sciences, South China Agricultural University, Guangzhou 510642, China. ; Mingzi Li:1.College of Marine Sciences, South China Agricultural University, Guangzhou 510642, China. ; Minjia Zhuang:1.College of Marine Sciences, South China Agricultural University, Guangzhou 510642, China. ; Yishuang Dai:1.College of Marine Sciences, South China Agricultural University, Guangzhou 510642, China. ; Kun Wu:1.Nansha-South China Agricultural University Fishery Research Institute, Guangzhou 510642, China; College of Marine Sciences, South China Agr
期刊:Characterization and function of PTEN-induced putative kinase 1 (PINK1) in process of Zinc alleviates hepatic lipid deposition of yellow catfish (Pelteobagrus fulvidraco)
DOI:10.1016/j.ijbiomac.2024.131156
影响因子 :8.2
引用产品: M199 培养基
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BDE-47-induced damage prevented by melatonin in grass carp hepatocytes (L8824) (2024-03-16)
作者:Jianhua Shan:1.College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People's Republic of China. ; Haoyue Guan:1.College of Animal Science and Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China. ; Zhuoqi Zhang:1.College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People's Republic of China. ; Wenxue Ma:1.College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People's Republic of China. ; Jingzeng Cai:1.College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People's Republic of China. ; 2.Key Laboratory of the Provincial Education Department of Heilongjiang for Common Animal Disease Prevention and Treatment, Harbin, 150030, People's Republic of China. ; Ge Gao:1.College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People's Republic of China. ; Ziwei Zhang:1.College of Veterinary Medicine, Northeast Agricultural
期刊:BDE-47-induced damage prevented by melatonin in grass carp hepatocytes (L8824)
DOI:10.1007/s11356-024-32856-3
影响因子 :5.8
引用产品: M199 培养基
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Ammonia‐‑nitrogen stress affects immune regulation via TNFα in yellow catfish (Pelteobagrus fulvidraco) (2024-01-18)
作者:Lanfei Xiao: 1.College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China;2.University Joint Laboratory of Guangdong Province, Hong Kong and Macao Region on Marine Bioresource Conservation and Exploitation, Guangzhou 510642, China; Chuan Chen: 1.College of Marine Sciences, South China Agricultural University, Guangzhou 510642, China;2.University Joint Laboratory of Guangdong Province, Hong Kong and Macao Region on Marine Bioresource Conservation and Exploitation, Guangzhou 510642, China; Yongjun Liang: 1.Guangdong Provincial Key Laboratory of Marine Biotechnology, Institute of Marine Sciences, Shantou University, Shantou 515063, China; Kun Wu: 1.College of Marine Sciences, South China Agricultural University, Guangzhou 510642, China;2.University Joint Laboratory of Guangdong Province, Hong Kong and Macao Region on Marine Bioresource Conservation and Exploitation, Guangzhou 510642, China; Xiaobo Wen: 1.College of Marine Sciences, South China Agricultura
期刊:Ammonia‐‑nitrogen stress affects immune regulation via TNFα in yellow catfish (Pelteobagrus fulvidraco)
DOI:10.1016/j.aquaculture.2024.740593
影响因子 :4.5
引用产品: M199 培养基
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Effects of Leonurine on oocyte maturation and parthenogenetic embryo development in sheep (2024-03-05)
作者:Panpan Wei:1.College of Animal Science and Technology, Shihezi University, Shihezi, China. ; Jing Wang:1.College of Animal Science and Technology, Shihezi University, Shihezi, China. ; Hengbin Yu:1.College of Animal Science and Technology, Shihezi University, Shihezi, China. ; Yan Chen:1.College of Animal Science and Technology, Shihezi University, Shihezi, China. ; Chang Liu:1.College of Animal Science and Technology, Shihezi University, Shihezi, China. ; Yue Zhang:1.College of Animal Science and Technology, Shihezi University, Shihezi, China. ; Weibin Zeng:1.College of Animal Science and Technology, Shihezi University, Shihezi, China. ; Guangdong Hu:1.College of Animal Science and Technology, Shihezi University, Shihezi, China. ;
期刊:Effects of Leonurine on oocyte maturation and parthenogenetic embryo development in sheep
影响因子 :1.7
引用产品: M199 培养基
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SARM suppresses TRIF, TRAF3, and IRF3/7 mediated antiviral signaling in large yellow croaker Larimichthys crocea (2023-01-13)
作者:Jia Xi Zhang:1.Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture and Rural Affairs, Ornamental Aquarium Engineering Research Centre in University of Fujian Province, Fisheries College, Jimei University, Xiamen, Fujian, China. ; Ying Li:1.Key Laboratory of Estuarine Ecological Security and Environmental Health, Tan Kah Kee College, Xiamen University, Zhangzhou, Fujian, China. ; Jun Chun Tang:1.Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture and Rural Affairs, Ornamental Aquarium Engineering Research Centre in University of Fujian Province, Fisheries College, Jimei University, Xiamen, Fujian, China. ; Kai Qing Li:1.College of the Environment and Ecology, Xiamen University, Xiamen, China. ; Juan Juan Shen:1.Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture and Rural Affairs, Ornamental Aquarium Engineering Research Centre in University of Fujian Province, Fisheries College, Ji
期刊:SARM suppresses TRIF, TRAF3, and IRF3/7 mediated antiviral signaling in large yellow croaker Larimichthys crocea
DOI:10.3389/fimmu.2022.1021443
影响因子 :7.3
引用产品: M199 培养基
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Monoclonal antibody targeting a novel linear epitope on nucleoprotein confers pan-reactivity to influenza A virus (2023-02-23)
作者:Min Gu:1.Animal Infectious Diseases Laboratory, College of Veterinary Medicine, Yangzhou University, 48 East Wenhui Road, Yangzhou, 225009, Jiangsu, China. ; 2.Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009, Jiangsu, China. ; 3.Jiangsu Key Laboratory of Zoonoses, Yangzhou University, Yangzhou, 225009, Jiangsu, China. ; Jun Jiao:1.Animal Infectious Diseases Laboratory, College of Veterinary Medicine, Yangzhou University, 48 East Wenhui Road, Yangzhou, 225009, Jiangsu, China. ; Suhan Liu:1.Animal Infectious Diseases Laboratory, College of Veterinary Medicine, Yangzhou University, 48 East Wenhui Road, Yangzhou, 225009, Jiangsu, China. ; Wanchen Zhao:1.Animal Infectious Diseases Laboratory, College of Veterinary Medicine, Yangzhou University, 48 East Wenhui Road, Yangzhou, 225009, Jiangsu, China. ; Zhichuang Ge:1.Animal Infectious Diseases Laboratory, College of Veterinary Medicine, Yangzhou University, 48
期刊:Monoclonal antibody targeting a novel linear epitope on nucleoprotein confers pan-reactivity to influenza A virus
DOI:10.1007/s00253-023-12433-3
影响因子 :5.0
引用产品: DMEM高糖 培养基 , M199 培养基
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Effect of RNA interference with HIF-1α on the growth of pulmonary artery endothelial cells in broiler chickens: JXAU-LP-HIF-1α (2023-12-18)
作者:Wen Peng:1.Jiangxi Provincial Key Laboratory for Animal Health, Institute of Animal Population Health, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, PR China. ; Weile Fang:1.Jiangxi Provincial Key Laboratory for Animal Health, Institute of Animal Population Health, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, PR China. ; Xiaona Gao:1.Jiangxi Provincial Key Laboratory for Animal Health, Institute of Animal Population Health, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, PR China. ; Xiaoquan Guo:1.Jiangxi Provincial Key Laboratory for Animal Health, Institute of Animal Population Health, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, PR China. ; Guyue Li:1.Jiangxi Provincial Key Laboratory for Animal Health, Institute of Animal Population Health, College of Animal Science and Technology, Jiangxi Agricul
期刊:Effect of RNA interference with HIF-1α on the growth of pulmonary artery endothelial cells in broiler chickens: JXAU-LP-HIF-1α
影响因子 :4.4
引用产品: M199 培养基
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Teleost TRAF7, a protein functions in the host antiviral responses via NF-κB and IRF3/7 mediated signaling (2023-02-06)
作者:Peng Tian Li: 1.Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture and Rural Affairs, Ornamental Aquarium Engineering Research Centre in University of Fujian Province, Fisheries College, Jimei University, Xiamen, Fujian, China; Ying Li: 1.Key Laboratory of Estuarine Ecological Security and Environmental Health, Tan Kah Kee College, Xiamen University, Zhangzhou, Fujian, China; Ying Chen: 1.Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture and Rural Affairs, Ornamental Aquarium Engineering Research Centre in University of Fujian Province, Fisheries College, Jimei University, Xiamen, Fujian, China; Zi Hao Luo: 1.Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture and Rural Affairs, Ornamental Aquarium Engineering Research Centre in University of Fujian Province, Fisheries College, Jimei University, Xiamen, Fujian, China; Yi Lei Wang: 1.Key Laboratory of Healthy Mariculture for the East
期刊:Teleost TRAF7, a protein functions in the host antiviral responses via NF-κB and IRF3/7 mediated signaling
DOI:10.3389/fmars.2023.1092732
影响因子 :3.7
引用产品: M199 培养基
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Putrescine alleviates the oxidative damage of cumulus-oocyte complex via improving fatty acid oxidation (2023-10-17)
作者:Hongyan Jia:1.State Key Laboratory of Reproductive Medicine, Clinical Center of Reproductive Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, China; Lianyungang Maternal and Child Health Hospital, Kangda College of Nanjing Medical University, Lianyungang City, 222000, China. ; Huaiyun Tang:1.Lianyungang Maternal and Child Health Hospital, Kangda College of Nanjing Medical University, Lianyungang City, 222000, China. ; Wei Wu:1.State Key Laboratory of Reproductive Medicine, Clinical Center of Reproductive Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, China. ; Zhengjie Yan:1.State Key Laboratory of Reproductive Medicine, Clinical Center of Reproductive Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, China. ; Chao Gao:1.State Key Laboratory of Reproductive Medicine, Clinical Center of Reproductive Medicine, The First Affiliated Hospital of Nanjing Medical University
期刊:Putrescine alleviates the oxidative damage of cumulus-oocyte complex via improving fatty acid oxidation
DOI:10.1016/j.bbrc.2023.149127
影响因子 :3.1
引用产品: M199 培养基
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Investigating the therapeutic effects and mechanisms of Roxadustat on peritoneal fibrosis Based on the TGF-β/Smad pathway (2023-12-14)
作者:Lingyun Wang:1.Department of Pharmacy, Nanjing Drum Tower Hospital Clinical College of Nanjing University of Chinese Medicine, China. ; Jiangqing Fan:1.Department of Pharmacy, China Pharmaceutical University Nanjing Drum Tower Hospital, China. ; Ting Yang:1.Department of Pharmacy, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University, China. ; Jizhong Shen:1.Department of Pharmacy, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University, China. Electronic address: sjzxlf@njglyy.com. ; Lulu Wang:1.Department of Pharmacy, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University, China; Hunan Provincial Key Laboratory of the Research and Development of Novel Pharmaceutical Preparations, The "Double-First Class" Application Characteristic Discipline of Hunan Province (Pharmaceutical Science), Changsha Medical University, Changsha, 410219, China. Electronic address: wanglulu2018@njglyy.com. ;
期刊:Investigating the therapeutic effects and mechanisms of Roxadustat on peritoneal fibrosis Based on the TGF-β/Smad pathway
DOI:10.1016/j.bbrc.2023.149387
影响因子 :3.1
引用产品: MET-5A 细胞 , MET-5A细胞专用培养基 , M199 培养基
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Melatonin relieves 2,2,4,4-tetrabromodiphenyl ether (BDE-47)-induced apoptosis and mitochondrial dysfunction through the AMPK-Sirt1-PGC-1α axis in fish kidney cells (CIK) (2002-02-20)
作者:Peixian Luan:1.Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 0150070, PR China; Key Laboratory of Freshwater Aquatic Biotechnology and Breeding, Ministry of Agriculture and Rural Affairs, Harbin 150070, PR China. ; Haoran Zhang:1.College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR China. ; Xiaoming Chen:1.College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR China. ; Yue Zhu:1.College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR China. ; Guo Hu:1.Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 0150070, PR China; Key Laboratory of Freshwater Aquatic Biotechnology and Breeding, Ministry of Agriculture and Rural Affairs, Harbin 150070, PR China. Electronic address: huguo@hrfri.ac.cn. ; Jingzeng Cai:1.College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR China. Electron
期刊:Melatonin relieves 2,2,4,4-tetrabromodiphenyl ether (BDE-47)-induced apoptosis and mitochondrial dysfunction through the AMPK-Sirt1-PGC-1α axis in fish kidney cells (CIK)
DOI:10.1016/j.ecoenv.2022.113276
影响因子 :6.8
引用产品: M199 培养基
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Small RNA sequencing reveals miRNAs important for hypoxic adaptation in the Tibetan chicken (2007-00-20)
作者:Z Zhang:1.Poultry Research Institute, Sichuan Animal Science Academy , Chengdu, Sichuan, China. ; 2.Poultry Research Institute, Animal Breeding and Genetics Key Laboratory of Sichuan Province , Chengdu, Sichuan, China. ; M Qiu:1.Poultry Research Institute, Sichuan Animal Science Academy , Chengdu, Sichuan, China. ; H Du:1.Poultry Research Institute, Sichuan Animal Science Academy , Chengdu, Sichuan, China. ; Q Li:1.Poultry Research Institute, Sichuan Animal Science Academy , Chengdu, Sichuan, China. ; C Yu:1.Poultry Research Institute, Sichuan Animal Science Academy , Chengdu, Sichuan, China. ; W Gan:1.Poultry Research Institute, Shanghai Ying Biotechnology Company , Shanghai, China. ; H Peng:1.Poultry Research Institute, Sichuan Animal Science Academy , Chengdu, Sichuan, China. ; B Xia:1.Poultry Research Institute, Sichuan Animal Science Academy , Chengdu, Sichuan, China. ; X Xiong:1.Poultry Research Institute, Sichuan Animal Science Academy , Chengdu, Sichuan, China. ; X Son
期刊:Small RNA sequencing reveals miRNAs important for hypoxic adaptation in the Tibetan chicken
DOI:10.1080/00071668.2020.1792835
影响因子 :2.0
引用产品: M199 培养基
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