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产品概述
Leibovitz's L-15培养基的缓冲系统由磷酸盐和游离碱基氨基酸组成，代替了传统的碳酸氢钠缓冲系统，同时，用半乳糖和丙酮酸钠代替葡萄糖可以防止酸性代谢副产物的形成，有助于维持培养液PH的稳定，适合用于非CO2平衡环境的细胞培养。Leibovitz's L-15培养基适用于猴肾细胞和HEP-2的培养、原代（如胚胎和成人组织）细胞分离。
酚红在培养基中被用来作为pH值的指示剂，用以持续监测培养液的酸碱度，在低pH值时酚红使培养液呈黄色，而较高的pH值时使培养液呈紫色，pH值7.2-7.4时为红色，最适合细胞培养。但酚红也有一些缺点，研究表明酚红可以模拟固醇类激素（特别是雌激素）的作用，因此在用到雌激素敏感的细胞时（如乳腺组织），最好使用不含酚红的培养基。酚红会干扰流式细胞分析时候的检测。此外，一些无血清培养基的配方中若存在酚红会干扰钠-钾平衡。
本产品含有多类细胞培养所需的氨基酸、维生素、无机盐等多种成分，但不含蛋白质、脂类或任何生长因子，故此产品需搭配血清或无血清添加物使用。
成分说明
形态 | 液体 |
浓度 | 1× |
规格 | 500mL |
pH | 7.1-7.8 |
缓冲系统 | 磷酸盐、氨基酸缓冲系统 |
L-谷氨酰胺 | 300 mg/L |
NaHCO3 | 无 |
D-葡萄糖 | 无 |
D-半乳糖 | 900 mg/L |
丙酮酸钠 | 550 mg/L |
HEPES缓冲剂 | 无 |
酚红指示剂 | 无 |
储存条件 | 2-8℃，避光 |
运输条件 | 常温 |
有效期 | 18个月 |
注意事项
1、本产品经0.1 μm过滤除菌，使用时应注意无菌操作，避免污染；
2、本品不适用于CO2环境，CO2会导致培养液PH降低（培养液变黄），严重影响细胞生长；
3、为保持本产品的最佳使用效果，请勿进行冻融处理；
4、本产品仅用于科研或进一步研究使用，不用于诊断和治疗。
参考文献
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The perturbation of parabens on the neuroendocrine system in zebrafish larvae (2023-04-21)
作者：Jiefeng Liang:1.State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; Sino-Danish College, University of Chinese Academy of Sciences, Beijing 100049, China; Shandong Key Laboratory of Environmental Processes and Health, School of Environmental Science and Engineering, Shandong University, Qingdao, 266237, China. ; Xiaoxi Yang:1.State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China. Electronic address: xxyang@rcees.ac.cn. ; Tongtong Xiang:1.State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; College of Science, Northeastern University, Shenyang 110004, China. ; Xuanyue Chen:1.State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research
期刊：The perturbation of parabens on the neuroendocrine system in zebrafish larvae
DOI：10.1016/j.scitotenv.2023.163593
影响因子 ：9.8
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3-tert-Butyl-4-hydroxyanisole perturbs renal lipid metabolism in vitro by targeting androgen receptor-regulated de novo lipogenesis (2023-05-05)
作者：Xiaoyun Wang:1.State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; College of Resources and Environment, University of Chinese Academy of Sciences, Beijing 100049, China. ; Zhendong Sun:1.State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; School of Environment, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou 310024, China. ; Yurou Gao:1.State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; Sino-Danish College, University of Chinese Academy of Sciences, Beijing 100049, China. ; Qian S Liu:1.State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences,
期刊：3-tert-Butyl-4-hydroxyanisole perturbs renal lipid metabolism in vitro by targeting androgen receptor-regulated de novo lipogenesis
DOI：10.1016/j.ecoenv.2023.114979
影响因子 ：6.8
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EMS1/DLL4-Notch Signaling Axis Augments Cell Cycle-Mediated Tumorigenesis and Progress in Human Adrenocortical Carcinoma (2011-01-20)
作者：Yu-Gang Huang:1.Department of Pathology, Taihe Hospital, Hubei University of Medicine, Shiyan, China. ; Ya Wang:1.Department of Immunology, Nankai University School of Medicine, Tianjin, China. ; Rui-Juan Zhu:1.Department of Pathology, Taihe Hospital, Hubei University of Medicine, Shiyan, China. ; Kai Tang:1.Department of Pediatric, Taihe Hospital, Hubei University of Medicine, Shiyan, China. ; Xian-Bin Tang:1.Department of Pathology, Taihe Hospital, Hubei University of Medicine, Shiyan, China. ; Xiao-Min Su:1.Department of Immunology, Nankai University School of Medicine, Tianjin, China. ;
期刊：EMS1/DLL4-Notch Signaling Axis Augments Cell Cycle-Mediated Tumorigenesis and Progress in Human Adrenocortical Carcinoma
影响因子 ：4.7
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