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产品概述
小鼠肺成纤维细胞
Cat NO.: CP-M006
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产品名称：小鼠肺成纤维细胞
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英文简称：LF
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组织来源：肺组织
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产品规格：5×10⁵Cells/T25培养瓶
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细胞简介：
小鼠肺成纤维细胞分离自肺组织；肺是机体的呼吸器官，位于胸腔，左右各一，覆盖于心之上。肺有分叶，左二右三，共五叶。肺经肺系(指气管、支气管等)与喉、鼻相连，故称喉为肺之门户，鼻为肺之外窍。成纤维细胞(Fibroblast)是疏松结缔组织的主要细胞成分，由胚胎时期的间充质细胞分化而来；成纤维细胞较大，轮廓清楚，多为突起的纺锤形或星形的扁平状结构，其细胞核呈规则的卵圆形，核仁大而明显。成纤维细胞功能活动旺盛，细胞质嗜弱碱性，具明显的蛋白质合成和分泌活动，在一定条件下，它可以实现跟纤维细胞的互相转化；成纤维细胞对不同程度的细胞变性、坏死和组织缺损的修复有着十分重要的作用。刚分离的肺成纤维细胞呈圆形、折光性良好，悬浮于培养基中。30min细胞贴壁，其中部分开始伸出伪足，表现为小的突起；6h后细胞基本贴壁完全，伸展成梭形，胞核清晰，分布较均匀，散在生长，不聚集成团；细胞生长迅速，5-7天即呈融合状态，细胞排列紧密，有的交叉重叠生长，平坦、胞体较大，细胞质透明，细胞核较大，呈椭圆形，颜色淡。细胞融合，并彼此连接成网状；细胞呈突起的纺锤形或星形的扁平分布。肺成纤维细胞在生理条件下的主要功能包括：构造和维持肺器官的正常形态，合成和释放细胞外基质以及组织损伤后及时大量聚集修复损伤组织。
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方法简介：
普诺赛实验室分离的小鼠肺成纤维细胞采用胰蛋白酶 - 胶原酶混合消化法结合差速贴壁法制备而来，细胞总量约为5×10⁵cells/瓶。
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质量检测：
普诺赛实验室分离的小鼠肺成纤维细胞经Vimentin免疫荧光鉴定，纯度可达90%以上，且不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等。
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培养信息：
包被条件 培养基 含FBS、生长添加剂、Penicillin、Streptomycin等 产品货号 CM-M006 换液频率 每2-3天换液一次 生长特性 贴壁 细胞形态 成纤维细胞样 传代特性 可传3-5代 消化液 0.25%胰蛋白酶 小鼠肺成纤维细胞体外培养周期有限；建议使用普诺赛配套的专用生长培养基及正确的操作方法来培养，以此保证该细胞的最佳培养状态。
参考文献
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Anoikis and SPP1 in idiopathic pulmonary fibrosis: integrating bioinformatics, cell, and animal studies to explore prognostic biomarkers and PI3K/AKT signaling regulation (2024-02-09)
作者：Yi Liao:1.Department of Respiratory and Critical Care Medicine, Sichuan Provincial People's Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, China. ; Yan Yang:1.Department of Respiratory and Critical Care Medicine, Sichuan Provincial People's Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, China. ; Guanghong Zhou:1.Department of Respiratory and Critical Care Medicine, Sichuan Provincial People's Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, China. ; Lijuan Chen:1.Department of Respiratory and Critical Care Medicine, Sichuan Provincial People's Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, China. ; Yang Yang:1.Department of Respiratory and Critical Care Medicine, Sichuan Provincial People's Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, C
期刊：Anoikis and SPP1 in idiopathic pulmonary fibrosis: integrating bioinformatics, cell, and animal studies to explore prognostic biomarkers and PI3K/AKT signaling regulation
DOI：10.1080/1744666X.2024.2315218
影响因子 ：4.4
引用产品： 小鼠肺成纤维细胞
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SiO2-induced ferroptosis in macrophages promotes the development of pulmonary fibrosis in silicosis models (2012-01-20)
作者：Taiyang Liu:1.School of Public Health and Management, Ningxia Medical University, No. 1160, Shengli Street, Xingqing District, Yinchuan 75000, Ningxia, China. ; Rui Bao:1.School of Public Health and Management, Ningxia Medical University, No. 1160, Shengli Street, Xingqing District, Yinchuan 75000, Ningxia, China. ; Qiushi Wang:1.School of Public Health and Management, Ningxia Medical University, No. 1160, Shengli Street, Xingqing District, Yinchuan 75000, Ningxia, China. ; Wei Hao:1.School of Public Health and Management, Ningxia Medical University, No. 1160, Shengli Street, Xingqing District, Yinchuan 75000, Ningxia, China. ; Yaoyang Liu:1.School of Public Health and Management, Ningxia Medical University, No. 1160, Shengli Street, Xingqing District, Yinchuan 75000, Ningxia, China. ; Sirong Chang:1.School of Public Health and Management, Ningxia Medical University, No. 1160, Shengli Street, Xingqing District, Yinchuan 75000, Ningxia, China. ; Meng Wang:1.School of Public Health
期刊：SiO2-induced ferroptosis in macrophages promotes the development of pulmonary fibrosis in silicosis models
影响因子 ：2.1
引用产品： 小鼠肺成纤维细胞
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