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产品概述
大鼠主动脉平滑肌细胞
Cat NO.: CP-R076
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产品名称：大鼠主动脉平滑肌细胞
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组织来源：主动脉组织
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产品规格：5×10⁵Cells/T25培养瓶
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细胞简介：
大鼠主动脉平滑肌细胞分离自主动脉组织；主动脉是体循环的动脉主干。其运行路径为：升主动脉起于左心室，至右侧第2胸肋关节高度移行为主动脉弓，弓行向左后至第4胸椎体下缘移行为降主动脉；在第12胸椎体高度穿膈的主动脉裂孔移行为腹主动脉，以上为胸主动脉，至第4腰椎体下缘分为左、右髂总动脉；髂总动脉在骶髂关节高度分为髂内、外动脉。主动脉平滑肌细胞原代分离培养3天后，可见细胞贴壁伸展，细胞形态大小不一，呈梭形、不规则形、三角形或扇形，核卵圆形、居中；2周后细胞汇合，多数细胞伸展呈长梭形，胞浆丰富，有分枝状突起，细胞平行排列成单层或部分区域多层重叠生长，高低起伏；细胞密度低时，常交织成网状；密度高时，则排列为旋涡状或栅栏状。传代后细胞生长较快，4-6天即可汇合，并保持上述形态学特征和生长特点。主动脉平滑肌细胞在心血管疾病发生、发展中具有重要作用，以主动脉平滑肌细胞为实验研究对象，探讨心血管疾病相关发病机制是目前研究的热点；体外培养的主动脉平滑肌细胞对于研究其生理功能、药物作用以及各种致病因素作用下的病理生理改变具重要意义。
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方法简介：
普诺赛实验室分离的大鼠主动脉平滑肌细胞采用胰蛋白酶 - 胶原酶联合消化法结合差速贴壁法制备而来，细胞总量约为5×10⁵cells/瓶。
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质量检测：
普诺赛实验室分离的大鼠主动脉平滑肌细胞经α-SMA免疫荧光鉴定，纯度可达90%以上，且不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等。
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培养信息：
包被条件 培养基 含FBS、生长添加剂、Penicillin、Streptomycin等 产品货号 CM-R076 换液频率 每2-3天换液一次 生长特性 贴壁 细胞形态 成纤维细胞样 传代特性 可传5代左右；3代以内状态最佳 消化液 0.25%胰蛋白酶 大鼠主动脉平滑肌细胞体外培养周期有限；建议使用普诺赛配套的专用生长培养基及正确的操作方法来培养，以此保证该细胞的最佳培养状态。
参考文献
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Investigating the anti-atherosclerotic effects and potential mechanism of Dalbergia odorifera in ApoE-deficient mice using network pharmacology combined with metabolomics (2024-02-07)
作者：Kang Sun:1.School of Pharmacy, Key Laboratory of Molecular Pharmacology and Drug Evaluation (Yantai University), Ministry of Education, Collaborative Innovation Center of Advanced Drug Delivery System and Biotech Drugs in Universities of Shandong, Yantai University, Yantai, China. ; Zongchao Li:1.School of Pharmacy, Key Laboratory of Molecular Pharmacology and Drug Evaluation (Yantai University), Ministry of Education, Collaborative Innovation Center of Advanced Drug Delivery System and Biotech Drugs in Universities of Shandong, Yantai University, Yantai, China. ; Wenjing Li:1.School of Pharmacy, Key Laboratory of Molecular Pharmacology and Drug Evaluation (Yantai University), Ministry of Education, Collaborative Innovation Center of Advanced Drug Delivery System and Biotech Drugs in Universities of Shandong, Yantai University, Yantai, China. ; Chenglin Chi:1.School of Pharmacy, Key Laboratory of Molecular Pharmacology and Drug Evaluation (Yantai University), Ministry of Education,
期刊：Investigating the anti-atherosclerotic effects and potential mechanism of Dalbergia odorifera in ApoE-deficient mice using network pharmacology combined with metabolomics
DOI：10.1016/j.jpba.2024.116017
影响因子 ：3.4
引用产品： 大鼠主动脉平滑肌细胞
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CDKN2B-AS1 mediates proliferation and migration of vascular smooth muscle cells induced by insulin (2023-11-01)
作者：Hao-Jie Jin:1.Department of Vascular Surgery, the Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325015, China. ; Zi-Heng Wu:1.Department of Vascular Surgery, School of Medicine, the First Affiliated Hospital, Zhejiang University, Hangzhou, 310003, China. ; Bao-Fu Zhang:1.Department of Vascular Surgery, the Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325015, China. ; Jie Deng:1.Department of Vascular Surgery, the Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325015, China. ; Yin-Dong Xu:1.Department of Vascular Surgery, the Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325015, China. ; Xin-Yu Wang:1.Institute of Ischemia-Reperfusion Injury, Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China. ; Zheng-Yang Song:1.Institute of Ischemia-Reperfusion Injury, Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China. ; Xin-Wu Lu:1.Department of Vascu
期刊：CDKN2B-AS1 mediates proliferation and migration of vascular smooth muscle cells induced by insulin
DOI：10.1007/s00441-023-03836-9
影响因子 ：3.6
引用产品： 大鼠主动脉平滑肌细胞
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