产品中心Cell Resources
联系我们CONTACT US
400-999-210024小时服务热线
人脐动脉平滑肌细胞
Human Umbilical Artery Smooth Muscle Cells
货号：CP-H085
规格：5×10⁵Cells/T25培养瓶
价格： ￥7380
培养基货号： CM-H085
产品概述
人脐动脉平滑肌细胞
Cat NO.: CP-H085
-
产品名称：人脐动脉平滑肌细胞
-
英文简称：UASMCs
-
组织来源：脐带组织
-
产品规格：5×10⁵Cells/T25培养瓶
-
细胞简介：
人脐动脉平滑肌细胞分离自脐带组织；它是脐动脉的重要结构组成细胞之一，在机体的正常生理过程中发挥着重要作用。脐带是哺乳类的连接胎儿和胎盘的管状结构，脐带中通过尿膜的血管即脐动脉和脐静脉，卵黄囊的血管即脐肠系膜动脉及脐肠系膜静脉。在子宫中，子宫动脉在胎盘的母体部分出的毛细血管，与胎盘的子体部胎儿毛细血管靠近，在此处母体和胎儿的血液间进行CO2和O2，代谢产物即代谢废物和营养物质的交换。脐动脉将胎儿产生的废物运送至胎盘，脐静脉将O2和营养物质从胎盘运送给胎儿。由于脐带是分娩过程中的废弃物，同时从脐带中分离脐静动平滑肌细胞方法相对成熟，使得体外培养的脐动脉平滑肌细胞作为研究血管的模型细胞。脐动脉平滑肌细胞原代分离培养3天后，可见细胞贴壁伸展，细胞形态大小不一，呈梭形、不规则形、三角形或扇形，核卵圆形、居中；2周后细胞汇合，多数细胞伸展呈长梭形，胞浆丰富，有分枝状突起，细胞平行排列成单层或部分区域多层重叠生长，高低起伏；细胞密度低时，常交织成网状；密度高时，则排列为旋涡状或栅栏状。传代后细胞生长较快，4-6天即可汇合，并保持上述形态学特征和生长特点。
-
方法简介：
普诺赛实验室分离的人脐动脉平滑肌细胞采用胰蛋白酶 - 胶原酶联合消化法结合差速贴壁法制备而来，细胞总量约为5×10⁵cells/瓶。
-
质量检测：
普诺赛实验室分离的人脐动脉平滑肌细胞经α-SMA免疫荧光鉴定，纯度可达90%以上，且不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等。
-
培养信息：
包被条件 培养基 含FBS、生长添加剂、Penicillin、Streptomycin等 产品货号 CM-H085 换液频率 每2-3天换液一次 生长特性 贴壁 细胞形态 成纤维细胞样 传代特性 可传5代左右；3代以内状态最佳 消化液 0.25%胰蛋白酶 人脐动脉平滑肌细胞体外培养周期有限；建议使用普诺赛配套的专用生长培养基及正确的操作方法来培养，以此保证该细胞的最佳培养状态。
参考文献
-
Dual-sized hollow particle incorporated fibroin thermal insulating coatings on catheter for cerebral therapeutic hypothermia (2023-02-24)
作者：Ming Li:1.China-America Institute of Neuroscience and Beijing Institute of Geriatrics, Xuanwu Hospital, Capital Medical University, Beijing, 100053, China. ; Yuan Gao:1.School of Instrumentation and Optoelectronic Engineering, Beihang University, Beijing, 100191, China. ; Miaowen Jiang:1.Beijing Institute for Brain Disorders, Capital Medical University, Beijing, 100069, China. ; Hongkang Zhang:1.School of Materials Science and Engineering, Peking University, Beijing, 100871, China. ; Yang Zhang:1.China-America Institute of Neuroscience and Beijing Institute of Geriatrics, Xuanwu Hospital, Capital Medical University, Beijing, 100053, China. ; Yan Wu:1.China-America Institute of Neuroscience and Beijing Institute of Geriatrics, Xuanwu Hospital, Capital Medical University, Beijing, 100053, China. ; Wenhao Zhou:1.Shaanxi Key Laboratory of Biomedical Metallic Materials, Northwest Institute for Nonferrous Metal Research, Xi'an, 710016, China. ; Di Wu:1.China-America Institute of Neuro
期刊：Dual-sized hollow particle incorporated fibroin thermal insulating coatings on catheter for cerebral therapeutic hypothermia
DOI：10.1016/j.bioactmat.2023.02.022
影响因子 ：18.9
引用产品： 人脐静脉内皮细胞 , 人脐动脉平滑肌细胞 , DMEM高糖 培养基 , RAW 264.7 细胞
-
Downregulation of connexin 43-based gap junctions underlies propofol-induced excessive relaxation in hypertensive vascular smooth muscle cells (2023-06-28)
作者：Weiqi Zeng:1.Department of Anesthesiology, Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, 510630, Guangdong, China. ; Zhizhao Deng:1.Department of Anesthesiology, Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, 510630, Guangdong, China. ; Yingxin Gao:1.Department of Anesthesiology, Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, 510630, Guangdong, China. ; Guoliang Sun:1.Department of Anesthesiology, Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, 510630, Guangdong, China. sunglsysu@qq.com. ; Xianlong Li:1.Department of Anesthesiology, Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, 510630, Guangdong, China. 286510848@qq.com. ; Dongdong Yuan:1.Department of Anesthesiology, Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, 510630, Guangdong, China. yuandongdong123@126.com. ;
期刊：Downregulation of connexin 43-based gap junctions underlies propofol-induced excessive relaxation in hypertensive vascular smooth muscle cells
DOI：10.1186/s12964-023-01176-3
影响因子 ：8.4
引用产品： 人脐动脉平滑肌细胞
-
Function of connexin 43 and RhoA/LIMK2/Cofilin signaling pathway in transient changes of contraction and dilation of human umbilical arterial smooth muscle cells (2022-10-28)
作者：Zhizhao Deng:1.Department of Anesthesiology, Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, Guangdong 510630, China. ; Yanling Zhang:1.Department of Anesthesiology, Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, Guangdong 510630, China. ; Qian Zhang:1.Department of Anesthesiology, Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, Guangdong 510630, China. ; Xianlong Li:1.Department of Anesthesiology, Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, Guangdong 510630, China. ; Weiqi Zeng:1.Department of Anesthesiology, Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, Guangdong 510630, China. ; Cai Jun:1.Department of Anesthesiology, Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, Guangdong 510630, China. Electronic address: gzcaijun@hotmail.com. ; Dongdong Yuan:1.Department of Anesthesiology, Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, Guangdong 510630, China. Electronic
期刊：Function of connexin 43 and RhoA/LIMK2/Cofilin signaling pathway in transient changes of contraction and dilation of human umbilical arterial smooth muscle cells
DOI：10.1016/j.biocel.2022.106326
影响因子 ：5.7
引用产品： 人脐动脉平滑肌细胞
FAQs
Q：{{item.question}}
A：
产品资料
识别码示意图
