产品中心Cell Resources
联系我们CONTACT US
400-999-210024小时服务热线
产品概述
人骨骼肌细胞
Cat NO.: CP-H095
-
产品名称：人骨骼肌细胞
-
组织来源：骨骼肌组织
-
产品规格：5×10⁵Cells/T25培养瓶
-
细胞简介：
人骨骼肌细胞分离自四肢肌肉组织；骨骼肌又称横纹肌，肌肉中的一种，约占全身重量的40%。骨骼肌纤维为长柱形的多核细胞，肌膜的外面有基膜紧密贴附。属于横纹肌，横纹肌还包括心肌与内脏横纹肌，其中骨骼肌主要分布于四肢。每块肌肉都是具有一定形态、结构和功能的器官，有丰富的血管、淋巴分布，在躯体神经支配下收缩或舒张，进行随意运动。肌肉可根据共形状、大小、位置、起止点、纤维方向和作用等命名。依形态命名的如斜方肌、菱形肌、三角肌、梨状肌等。骨骼肌细胞呈纤维状，不分支，有明显横纹，核很多，且都位于细胞膜下方。肌细胞内有许多沿细胞长轴平行排列的细丝状肌原纤维。每一肌原纤维都有相间排列的明带(Ⅰ带)及暗带(A带)。明带染色较浅，而暗带染色较深。暗带中间有一条较明亮的线称H线。H线的中部有一M线。明带中间，有一条较暗的线称为Z线。两个Z线之间的区段，叫做一个肌节。骨骼肌细胞也称横纹肌细胞，细胞呈纤维状，不分支，有明显横纹，核很多，且都位于细胞膜下方，细胞多呈梭行，具有一定的方向性。骨骼肌细胞原代分离培养3天后，可见细胞贴壁伸展，细胞形态大小不一，呈梭形、不规则形、三角形或扇形，核卵圆形、居中；2周后细胞汇合，多数细胞伸展呈长梭形，胞浆丰富，有分枝状突起，细胞平行排列成单层或部分区域多层重叠生长，高低起伏；细胞密度低时，常交织成网状；密度高时，则排列为旋涡状或栅栏状。传代后细胞生长较快，4-6天即可汇合，并保持上述形态学特征和生长特点。
-
方法简介：
普诺赛实验室分离的人骨骼肌细胞采用胶原酶消化法制备而来，细胞总量约为5×10⁵cells/瓶。
-
质量检测：
普诺赛实验室分离的人骨骼肌细胞经α-Sarcomeric actin免疫荧光鉴定，纯度可达90%以上，且不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等。
-
培养信息：
包被条件 PLL(0.1mg/ml) 培养基 含FBS、生长添加剂、Penicillin、Streptomycin等 产品货号 CM-H095 换液频率 每2-3天换液一次 生长特性 贴壁 细胞形态 成纤维细胞样 传代特性 可传3代左右 消化液 0.25%胰蛋白酶 人骨骼肌细胞体外培养周期有限；建议使用普诺赛配套的专用生长培养基及正确的操作方法来培养，以此保证该细胞的最佳培养状态。
参考文献
-
Antibodies against SARS-CoV-2 non-structural protein 3 cross-react with human muscle cells and neuroglial cells (2024-01-27)
作者：Xin-Yue Yang:1.College of Resources, Sichuan Agricultural University, Chengdu 611130, China. ; Ting Liu:1.Sichuan Kelun Pharmaceutical Comp. Ltd., Chengdu 610071, China. ; Si-Cong Jiang:1.Haisco Pharmaceutical Group Comp. Ltd., Chengdu 611138, China. ; Zhong-Wei Zhang:1.College of Resources, Sichuan Agricultural University, Chengdu 611130, China. ; Yu-Fan Fu:1.College of Resources, Sichuan Agricultural University, Chengdu 611130, China. ; Zi-Lin Li:1.Department of Cardiovascular Surgery, Xijing Hospital, Medical University of the Air Force, Xi'an 710032, China. ; Jing Hu:1.School of Medicine, Northwest University, Xi'an 710069, China. ; Shu Yuan:1.College of Resources, Sichuan Agricultural University, Chengdu 611130, China. Electronic address: roundtree318@hotmail.com. ;
期刊：Antibodies against SARS-CoV-2 non-structural protein 3 cross-react with human muscle cells and neuroglial cells
DOI：10.1016/j.vaccine.2024.01.074
影响因子 ：5.5
引用产品： 293T [HEK-293T] 细胞 , 人骨骼肌细胞 , 人神经星形胶质细胞
-
KIF15 upregulation promotes leiomyosarcoma cell growth via promoting USP15-mediated DEK deubiquitylation (2007-01-20)
作者：Weiming Ge:1.Foot and Ankle Surgery, Luoyang Orthopedic Hospital of Henan Province, Orthopedic Hospital of Henan Province, Luoyang, Henan, China. ; Yuxuan Chen:1.Center of Traumatic Orthopedics, People's Liberation Army 990 Hospital, Xinyang, Henan, China. ; Yusheng Guo:1.Foot and Ankle Surgery, Luoyang Orthopedic Hospital of Henan Province, Orthopedic Hospital of Henan Province, Luoyang, Henan, China. ; Dawei Zhao:1.Foot and Ankle Surgery, Luoyang Orthopedic Hospital of Henan Province, Orthopedic Hospital of Henan Province, Luoyang, Henan, China. ; Ling Mu:1.Foot and Ankle Surgery, Luoyang Orthopedic Hospital of Henan Province, Orthopedic Hospital of Henan Province, Luoyang, Henan, China. ; Kun Zhang:1.Foot and Ankle Surgery, Luoyang Orthopedic Hospital of Henan Province, Orthopedic Hospital of Henan Province, Luoyang, Henan, China. ; Wenkun Zhuo:1.Department of Orthopedics and Traumatology, 960 Hospital of PLA, Jinan, Shandong, China. Electronic address: wenkunzhuo@21cn.com. ;
期刊：KIF15 upregulation promotes leiomyosarcoma cell growth via promoting USP15-mediated DEK deubiquitylation
DOI：10.1016/j.bbrc.2021.07.042
影响因子 ：3.1
引用产品： 人骨骼肌细胞
-
USF1 promotes the development of knee osteoarthritis by activating the NF‑κB signaling pathway (2008-08-20)
作者：Xiandong Song:1.Department of Orthopedics, Hongqi Hospital Affiliated with Mudanjiang Medical University, Mudanjiang, Heilongjiang 157011, P.R. China. ; Min Zhu:1.Department of Radiology, Hongqi Hospital Affiliated with Mudanjiang Medical University, Mudanjiang, Heilongjiang 157011, P.R. China. ; Hao Li:1.Department of Radiology, Hongqi Hospital Affiliated with Mudanjiang Medical University, Mudanjiang, Heilongjiang 157011, P.R. China. ; Bo Liu:1.Department of Orthopedics, Hongqi Hospital Affiliated with Mudanjiang Medical University, Mudanjiang, Heilongjiang 157011, P.R. China. ; Zhaowei Yan:1.Department of Orthopedics, Hongqi Hospital Affiliated with Mudanjiang Medical University, Mudanjiang, Heilongjiang 157011, P.R. China. ; Weican Wang:1.Department of Orthopedics, Hongqi Hospital Affiliated with Mudanjiang Medical University, Mudanjiang, Heilongjiang 157011, P.R. China. ; Hongyi Li:1.Department of Radiology, Hongqi Hospital Affiliated with Mudanjiang Medical University, Muda
期刊：USF1 promotes the development of knee osteoarthritis by activating the NF‑κB signaling pathway
影响因子 ：2.7
引用产品： 人骨骼肌细胞 , 人骨骼肌细胞完全培养基
FAQs
Q：{{item.question}}
A：
产品资料
识别码示意图
