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人脑动脉血管平滑肌细胞
Human Brain Artery Vascular Smooth Muscle Cells
货号：CP-H116
规格：5×10⁵Cells/T25培养瓶
价格： ￥8880
培养基货号： CM-H116
产品概述
人脑动脉血管平滑肌细胞
Cat NO.: CP-H116
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产品名称：人脑动脉血管平滑肌细胞
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组织来源：脑动脉组织
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产品规格：5×10⁵Cells/T25培养瓶
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细胞简介：
人脑动脉血管平滑肌细胞分离自脑动脉组织；脑动脉有成对的颈内动脉和椎动脉互相衔接成动脉循环；静脉系多不与同名动脉拌行，所收集的静脉血先进入静脉窦再汇入颈内静脉；各级静脉都没有瓣膜。它包括脑的动脉系统和脑的静脉系统。脑动脉平滑肌细胞原代分离培养3天后，可见细胞贴壁伸展，细胞形态大小不一，呈梭形、不规则形、三角形或扇形，核卵圆形、居中；2周后细胞汇合，多数细胞伸展呈长梭形，胞浆丰富，有分枝状突起，细胞平行排列成单层或部分区域多层重叠生长，高低起伏；细胞密度低时，常交织成网状；密度高时，则排列为旋涡状或栅栏状。传代后细胞生长较快，4-6天即可汇合，并保持上述形态学特征和生长特点。
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方法简介：
普诺赛实验室分离的人脑动脉平滑肌细胞采用胰蛋白酶 - 胶原酶联合消化法结合差速贴壁法制备而来，细胞总量约为5×10⁵cells/瓶。
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质量检测：
普诺赛实验室分离的人脑动脉血管平滑肌细胞经α-SMA免疫荧光鉴定，纯度可达90%以上，且不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等。
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培养信息：
包被条件 培养基 含FBS、生长添加剂、Penicillin、Streptomycin等 产品货号 CM-H116 换液频率 每2-3天换液一次 生长特性 贴壁 细胞形态 成纤维细胞样 传代特性 可传5代左右；3代以内状态最佳 消化液 0.25%胰蛋白酶 人脑动脉血管平滑肌细胞体外培养周期有限；建议使用普诺赛配套的专用生长培养基及正确的操作方法来培养，以此保证该细胞的最佳培养状态。
参考文献
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Artesunate inhibits macrophage-like phenotype switching of vascular smooth muscle cells and attenuates vascular inflammatory injury in atherosclerosis via NLRP3 (2024-02-06)
作者：Ping Liu:1.Key Laboratory of Basic Pharmacology of Ministry of Education and Joint International Research Laboratory of Ethnomedicine of Ministry of Education, Zunyi Medical University, Zunyi 563000, China. ; Yuqi Wang:1.Key Laboratory of Basic Pharmacology of Ministry of Education and Joint International Research Laboratory of Ethnomedicine of Ministry of Education, Zunyi Medical University, Zunyi 563000, China. ; Keke Tian:1.Key Laboratory of Basic Pharmacology of Ministry of Education and Joint International Research Laboratory of Ethnomedicine of Ministry of Education, Zunyi Medical University, Zunyi 563000, China. ; Xinyu Bai:1.Key Laboratory of Basic Pharmacology of Ministry of Education and Joint International Research Laboratory of Ethnomedicine of Ministry of Education, Zunyi Medical University, Zunyi 563000, China. ; Yaowen Wang:1.Department of Cardiology, the Second Affiliated Hospital of Chongqing Medical University, Chongqing Cardiac Arrhythmias Therapeutic Service Cen
期刊：Artesunate inhibits macrophage-like phenotype switching of vascular smooth muscle cells and attenuates vascular inflammatory injury in atherosclerosis via NLRP3
DOI：10.1016/j.biopha.2024.116255
影响因子 ：7.5
引用产品： 人脑动脉血管平滑肌细胞
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PRMT3 methylates HIF-1α to enhance the vascular calcification induced by chronic kidney disease (2024-01-10)
作者：Guangyu Zhou:1.Department of Nephrology, Shengjing Hospital of China Medical University, 36# Sanhao Street, Shenyang, China. ; Chen Zhang:1.Department of Nephrology, Shengjing Hospital of China Medical University, 36# Sanhao Street, Shenyang, China. ; Hui Peng:1.Department of Nephrology, Shengjing Hospital of China Medical University, 36# Sanhao Street, Shenyang, China. ; Xuesong Su:1.Department of Nephrology, Shengjing Hospital of China Medical University, 36# Sanhao Street, Shenyang, China. ; Qun Huang:1.Department of Nephrology, Shengjing Hospital of China Medical University, 36# Sanhao Street, Shenyang, China. ; Zixia Zhao:1.Department of Nephrology, Shengjing Hospital of China Medical University, 36# Sanhao Street, Shenyang, China. ; Guangyi Zhao:1.Department of Anesthesiology, Shengjing Hospital of China Medical University, 36# Sanhao Street, Shenyang, 110004, China. zhaoguangyi110004@163.com. ;
期刊：PRMT3 methylates HIF-1α to enhance the vascular calcification induced by chronic kidney disease
DOI：10.1186/s10020-023-00759-7
影响因子 ：5.7
引用产品： 人脑动脉血管平滑肌细胞
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MTMR7 suppresses the phenotypic switching of vascular smooth muscle cell and vascular intimal hyperplasia after injury via regulating p62/mTORC1-mediated glucose metabolism (2024-02-01)
作者：Xiongshan Sun:1.Department of Cardiology, The General Hospital of Western Theater Command, Chengdu, Sichuan, China. ; Yao Yang:1.From the Department of Pharmacy, The General Hospital of Western Theater Command, Chengdu, Sichuan, China. ; Weiwei Zhao:1.Department of Cardiology, The General Hospital of Western Theater Command, Chengdu, Sichuan, China. ; Mingliang Wang:1.Department of Cardiology, The General Hospital of Western Theater Command, Chengdu, Sichuan, China. ; Yingmei Chen:1.Department of Cardiology, The General Hospital of Western Theater Command, Chengdu, Sichuan, China. ; Jia Wang:1.Department of Cardiology, The General Hospital of Western Theater Command, Chengdu, Sichuan, China. ; Dachun Yang:1.Department of Cardiology, The General Hospital of Western Theater Command, Chengdu, Sichuan, China. Electronic address: yangdc71@126.com. ; Yongjian Yang:1.Department of Cardiology, The General Hospital of Western Theater Command, Chengdu, Sichuan, China. Electronic address:
期刊：MTMR7 suppresses the phenotypic switching of vascular smooth muscle cell and vascular intimal hyperplasia after injury via regulating p62/mTORC1-mediated glucose metabolism
DOI：10.1016/j.atherosclerosis.2024.117470
影响因子 ：5.3
引用产品： 人脑动脉血管平滑肌细胞
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Effects of KLF11 on Vascular Smooth Muscle Cells and its Underlying Mechanisms in Intracranial Aneurysm (2024-02-18)
作者：Ke Yan:1.Department of Neurosurgery, Clinical Medical College of Yangzhou University, Northern Jiangsu People's Hospital, No. 98 Nantong West Road, Yangzhou, 225001, China. ; Jiarong Bian:1.Department of Respiratory Medicine, Clinical Medical College of Yangzhou University, Northern Jiangsu People's Hospital, Yangzhou, 225001, China. ; Liang He:1.Department of Neurosurgery, Clinical Medical College of Yangzhou University, Northern Jiangsu People's Hospital, No. 98 Nantong West Road, Yangzhou, 225001, China. ; Bingwei Song:1.Department of Neurosurgery, Clinical Medical College of Yangzhou University, Northern Jiangsu People's Hospital, No. 98 Nantong West Road, Yangzhou, 225001, China. ; Linhai Shen:1.Department of Neurosurgery, Clinical Medical College of Yangzhou University, Northern Jiangsu People's Hospital, No. 98 Nantong West Road, Yangzhou, 225001, China. ; Yong Zhen:1.Department of Neurosurgery, Clinical Medical College of Yangzhou University, Northern Jiangsu People's Hosp
期刊：Effects of KLF11 on Vascular Smooth Muscle Cells and its Underlying Mechanisms in Intracranial Aneurysm
DOI：10.1007/s10528-024-10681-0
影响因子 ：2.4
引用产品： 人脑动脉血管平滑肌细胞 , DMEM高糖 培养基 , 特级胎牛血清 , 青霉素-链霉素溶液 (双抗)，100×
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MiR-3646 accelerates inflammatory response of Ang II-induced hVSMCs via CYP2J2/EETs axis in hypertension model (2023-02-07)
作者：Runzhi Liu:1.Department of Geriatrics, The Third Hospital of Changsha City, Changsha, Hunan Province, P.R. China. ; Liying Zhong:1.Department of Geriatrics, The Third Hospital of Changsha City, Changsha, Hunan Province, P.R. China. ; Cong Wang:1.Department of Geriatrics, The Third Hospital of Changsha City, Changsha, Hunan Province, P.R. China. ; Yehai Sun:1.Department of Geriatrics, The Third Hospital of Changsha City, Changsha, Hunan Province, P.R. China. ; Wunjuan Ru:1.Department of Geriatrics, The Third Hospital of Changsha City, Changsha, Hunan Province, P.R. China. ; Wei Dai:1.Department of Geriatrics, The Third Hospital of Changsha City, Changsha, Hunan Province, P.R. China. ; Shengnan Yang:1.Department of Geriatrics, The Third Hospital of Changsha City, Changsha, Hunan Province, P.R. China. ; Aimin Zhong:1.Department of Geriatrics, The Third Hospital of Changsha City, Changsha, Hunan Province, P.R. China. ; XiuMei Xie:1.Department of Geriatrics, Xiangya Hospital, Centra
期刊：MiR-3646 accelerates inflammatory response of Ang II-induced hVSMCs via CYP2J2/EETs axis in hypertension model
DOI：10.1080/10641963.2023.2166948
影响因子 ：12.3
引用产品： 人脑动脉血管平滑肌细胞
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JMJD3 is Involved in Intracranial Aneurysm Development by Regulating DLX2 Expression through H3K27me3 Modification (2023-09-20)
作者：Xuan Wang:1.Department of Clinical Laboratory, Laohekou No.1 Hospital. ; Nan Li:1.Department of Pharmacy, Xiangyang Central Hospita, Affiliated Hospital of Hubei University of Arts and Science. ; Tong Li:1.Department of Neurology, Xiangyang Central Hospital, Affiliated Hospital of Hubei University of Arts and Science. ;
期刊：JMJD3 is Involved in Intracranial Aneurysm Development by Regulating DLX2 Expression through H3K27me3 Modification
影响因子 ：2.2
引用产品： 人脑动脉血管平滑肌细胞
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CircRNA DOCK1 Regulates miR-409-3p/MCL1 Axis to Modulate Proliferation and Apoptosis of Human Brain Vascular Smooth Muscle Cells (2005-01-20)
作者：Xinmin Ding:1.Department of Neurosurgery, Shanxi Bethune Hospital, The Third Hospital of Shanxi Medical University, Taiyuan, China. ; Xiaolong Wang:1.Department of Neurosurgery, Shanxi Bethune Hospital, The Third Hospital of Shanxi Medical University, Taiyuan, China. ; Li Han:1.Department of Neurosurgery, Shanxi Bethune Hospital, The Third Hospital of Shanxi Medical University, Taiyuan, China. ; Zhiyu Zhao:1.Department of Neurosurgery, Shanxi Bethune Hospital, The Third Hospital of Shanxi Medical University, Taiyuan, China. ; Shuai Jia:1.Department of Neurosurgery, Shanxi Bethune Hospital, The Third Hospital of Shanxi Medical University, Taiyuan, China. ; Yuanzhao Tuo:1.Department of Neurosurgery, Shanxi Bethune Hospital, The Third Hospital of Shanxi Medical University, Taiyuan, China. ;
期刊：CircRNA DOCK1 Regulates miR-409-3p/MCL1 Axis to Modulate Proliferation and Apoptosis of Human Brain Vascular Smooth Muscle Cells
影响因子 ：5.5
引用产品： 人脑动脉血管平滑肌细胞
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