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产品概述
大鼠视网膜神经节细胞
Cat NO.: CP-R122
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产品名称：大鼠视网膜神经节细胞
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英文简称：RGCs
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组织来源：视网膜组织
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产品规格：5×10⁵Cells/T25培养瓶
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细胞简介：
大鼠视网膜神经节细胞分离自视网膜组织；视网膜居于眼球壁的内层，是一层透明的薄膜。视网膜由色素上皮层和视网膜感觉层组成，两层间在病理情况下可分开，称为视网膜脱离。色素上皮层与脉络膜紧密相连，由色素上皮细胞组成，它们具有支持和营养光感受器细胞、遮光、散热以及再生和修复等作用。组织学上视网膜分为10层，由外向内分别为：色素上皮层、视锥、视杆细胞层、外界膜、外颗粒层、外丛状层、内颗粒层、内丛状层、神经节细胞层、神经纤维层、内界膜。视网膜内层为衬于血管膜内面的一层薄膜，有感光作用；后部鼻侧有一视神经乳头。视网膜上的感觉层是由三个神经元组成。第一神经元是视细胞层，专司感光，它包括锥细胞和杆细胞。视杆细胞主要在离中心凹较远的视网膜上，而视锥细胞则在中心凹处最多。第二层叫双节细胞，约有10到数百个视细胞通过双节细胞与一个神经节细胞相联系，负责联络作用。第三层叫节细胞层，专管传导。视网膜是一层菲薄的但又非常复杂的结构，它贴于眼球的后壁部，传递来自视网膜感受器冲动的神经纤维跨越视网膜表面，经由视神经到达出口。视网膜的分辨力是不均匀的，在黄斑区，其分辨能力最强。视网膜神经节细胞贴壁后呈单层排列，部分细胞聚集成团，细胞呈圆形或椭圆形，核圆且相对透明，有些细胞膜上伸出短而小的突起；该细胞为高度分化细胞，在体外属于不增殖群。视网膜神经节细胞是青光眼病理性损害的主要细胞，视网膜神经节细胞的死亡可导致视功能不可逆损伤，研究视网膜神经节细胞损害的细胞学和分子生物学机制有利于青光眼发病机制的研究。
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方法简介：
普诺赛实验室分离的大鼠视网膜神经节细胞采用胰蛋白酶-胶原酶联合消化法，结合视网膜神经节细胞专用培养基培养和化学试剂抑制法筛选制备而来，细胞总量约为5×10⁵cells/瓶。
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质量检测：
普诺赛实验室分离的大鼠视网膜神经节细胞经CD90免疫荧光鉴定，纯度可达90%以上，且不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等。
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培养信息：
包被条件 PLL(0.1mg/ml) 培养基 基础培养基，含FBS、B-27 Supplement、Penicillin、Streptomycin等 产品货号 CM-R122 换液频率 每2-3天换液一次 生长特性 贴壁 细胞形态 神经元细胞样 传代特性 属于终末分化细胞；属于不增殖细胞群 消化液 0.25%胰蛋白酶 大鼠视网膜神经节细胞体外培养周期有限；建议使用普诺赛配套的专用生长培养基及正确的操作方法来培养，以此保证该细胞的最佳培养状态。
参考文献
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Tongqiao Mingmu formula alleviates retinal ganglion cell autophagy through PI3K/AKT/mTOR pathway (2022-09-13)
作者：Liyuan Wang:1.Heilongjiang University of Chinese Medicine, Harbin, China. ; 2.Department of Ophthalmology, First Affiliated Hospital of Heilongjiang University of Chinese Medicine, Harbin, China. ; 3.Heilongjiang Academy of Sciences of Traditional Chinese Medicine, Harbin, China. ; Tianyang Yu:1.Heilongjiang University of Chinese Medicine, Harbin, China. ; 2.Department of Acupuncture, Second Affiliated Hospital of Heilongjiang University of Chinese Medicine, Harbin, China. ; Feixue Dong:1.Heilongjiang University of Chinese Medicine, Harbin, China. ; 2.Department of Ophthalmology, First Affiliated Hospital of Heilongjiang University of Chinese Medicine, Harbin, China. ; Jiayu Xu:1.Heilongjiang University of Chinese Medicine, Harbin, China. ; Jin Fu:1.Heilongjiang University of Chinese Medicine, Harbin, China. ; He Sun:1.Heilongjiang University of Chinese Medicine, Harbin, China. ; 2.Department of Ophthalmology, First Affiliated Hospital of Heilongjiang University of Chinese Medicin
期刊：Tongqiao Mingmu formula alleviates retinal ganglion cell autophagy through PI3K/AKT/mTOR pathway
DOI：10.1002/ar.25060
影响因子 ：2.2
引用产品： 大鼠视网膜神经节细胞
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An adherent drug depot for retinal ganglion cell protection and regeneration in rat traumatic optic neuropathy models (2006-01-20)
作者：Lingli Li:1.School of Ophthalmology & Optometry, Affiliated Eye Hospital, Wenzhou Medical University Zhejiang Province P. R. China nankh@163.com. ; 2.State Key Laboratory of Ophthalmology, Optometry and Visual Science Zhejiang Province P. R. China. ; Fen Deng:1.School of Ophthalmology & Optometry, Affiliated Eye Hospital, Wenzhou Medical University Zhejiang Province P. R. China nankh@163.com. ; 2.The 2nd Affiliated Hospital and Yuying Children's Hospital, Wenzhou Medical University Zhejiang Province P. R. China. ; Haijun Qiu:1.School of Ophthalmology & Optometry, Affiliated Eye Hospital, Wenzhou Medical University Zhejiang Province P. R. China nankh@163.com. ; 2.State Key Laboratory of Ophthalmology, Optometry and Visual Science Zhejiang Province P. R. China. ; Yao Li:1.School of Ophthalmology & Optometry, Affiliated Eye Hospital, Wenzhou Medical University Zhejiang Province P. R. China nankh@163.com. ; 2.State Key Laboratory of Ophthalmology, Optometry and Visual Science Zhejiang P
期刊：An adherent drug depot for retinal ganglion cell protection and regeneration in rat traumatic optic neuropathy models
影响因子 ：3.9
引用产品： 大鼠视网膜神经节细胞
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Localized co-delivery of CNTF and FK506 using a thermosensitive hydrogel for retina ganglion cells protection after traumatic optic nerve injury (2004-00-20)
作者：Dongmei Wang:1.School of Ophthalmology & Optometry and Eye Hospital, Wenzhou Medical University, Wenzhou, China. ; Mengmeng Luo:1.School of Ophthalmology & Optometry and Eye Hospital, Wenzhou Medical University, Wenzhou, China. ; 2.Wenzhou Institute, University of Chinese Academy of Science, Wenzhou, China. ; Baoshan Huang:1.School of Ophthalmology & Optometry and Eye Hospital, Wenzhou Medical University, Wenzhou, China. ; 2.Wenzhou Institute, University of Chinese Academy of Science, Wenzhou, China. ; Wa Gao:1.School of Ophthalmology & Optometry and Eye Hospital, Wenzhou Medical University, Wenzhou, China. ; Yan Jiang:1.School of Ophthalmology & Optometry and Eye Hospital, Wenzhou Medical University, Wenzhou, China. ; Qing Li:1.School of Ophthalmology & Optometry and Eye Hospital, Wenzhou Medical University, Wenzhou, China. ; 2.State Key Laboratory of Ophthalmology, Optometry and Visual Science, Wenzhou Medical University, Wenzhou, China. ; Kaihui Nan:1.School of Ophthalmology &
期刊：Localized co-delivery of CNTF and FK506 using a thermosensitive hydrogel for retina ganglion cells protection after traumatic optic nerve injury
DOI：10.1080/10717544.2020.1748759
影响因子 ：6.0
引用产品： 大鼠视网膜神经节细胞
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Sestrin2 overexpression alleviates hydrogen peroxide-induced apoptosis and oxidative stress in retinal ganglion cells by enhancing Nrf2 activation via Keap1 downregulation (2004-00-20)
作者：Yazhi Fan:1.Department of Ophthalmology, The Second Affiliated Hospital of Xi'an Jiaotong University, 157 Xiwu Road, Xi'an, Shaanxi, 710004, China. Electronic address: fanyazhi8227@163.com. ; Yao Xing:1.Department of Ophthalmology, The Second Affiliated Hospital of Xi'an Jiaotong University, 157 Xiwu Road, Xi'an, Shaanxi, 710004, China. ; Lei Xiong:1.Department of Ophthalmology, The Second Affiliated Hospital of Xi'an Jiaotong University, 157 Xiwu Road, Xi'an, Shaanxi, 710004, China. ; Jianming Wang:1.Department of Ophthalmology, The Second Affiliated Hospital of Xi'an Jiaotong University, 157 Xiwu Road, Xi'an, Shaanxi, 710004, China. ;
期刊：Sestrin2 overexpression alleviates hydrogen peroxide-induced apoptosis and oxidative stress in retinal ganglion cells by enhancing Nrf2 activation via Keap1 downregulation
影响因子 ：5.1
引用产品： 大鼠视网膜神经节细胞
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Silencing of GAS5 Alleviates Glaucoma in Rat Models by Reducing Retinal Ganglion Cell Apoptosis (2011-09-20)
作者：Rong-Rong Zhou:1.Department of Oncology, Xiangya Hospital, Central South University, Changsha, China. ; Hai-Bo Li:1.Department of Ophthalmology, Xiangya Hospital, Central South University, Changsha, China. ; Qi-Sheng You:1.Casey Eye Institute, Oregon Health Science University, Portland, Oregon. ; Rong Rong:1.Department of Ophthalmology, Xiangya Hospital, Central South University, Changsha, China. ; Meng-Ling You:1.Department of Ophthalmology, Xiangya Hospital, Central South University, Changsha, China. ; Kun Xiong:1.Department of Anatomy and Neurobiology, School of Basic Medical Sciences, Central South University, Changsha, China. ; Ju-Fang Huang:1.Department of Anatomy and Neurobiology, School of Basic Medical Sciences, Central South University, Changsha, China. ; Xiao-Bo Xia:1.Department of Ophthalmology, Xiangya Hospital, Central South University, Changsha, China. ; Dan Ji:1.Department of Ophthalmology, Xiangya Hospital, Central South University, Changsha, China. ;
期刊：Silencing of GAS5 Alleviates Glaucoma in Rat Models by Reducing Retinal Ganglion Cell Apoptosis
影响因子 ：4.2
引用产品： 大鼠视网膜神经节细胞
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