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产品概述
大鼠海绵体内皮细胞
Cat NO.: CP-R133
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产品名称：大鼠海绵体内皮细胞
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组织来源：海绵体组织
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产品规格：5×10⁵Cells/T25培养瓶
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细胞简介：
大鼠海绵体内皮细胞分离自海绵体组织；阴茎海绵体是由海绵状的小梁和腔隙构成的血窦结构，它主要包括海绵体内皮细胞、平滑肌细胞和成纤维细胞3种细胞成分。其中平滑肌细胞和成纤维细胞镶嵌于海绵体细胞外基质的胶原中，而海绵体内皮细胞则覆盖于海绵体血窦的腔面，仅占海绵体组织的2.8%。在消化海绵体组织时，胶原酶的作用主要是松解海绵体内皮细胞与基膜的联系，破坏海绵体内皮细胞间的紧密连接。如果消化时间延长或胶原酶浓度过大，平滑肌细胞和成纤维细胞也将被消化下来，从而影响海绵体内皮细胞的纯度。因此，比较筛选合适的胶原酶浓度和消化时间是成功分离海绵体内皮细胞的关键。
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方法简介：
普诺赛实验室分离的大鼠海绵体内皮细胞采用混合酶消化法结合机械分离法、并用内皮细胞专用培养基培养筛选制备而来，细胞总量约为5×10⁵cells/瓶。
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质量检测：
普诺赛实验室分离的大鼠海绵体内皮细胞经CD31免疫荧光鉴定，纯度可达90%以上，且不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等。
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培养信息：
包被条件 PLL（0.1mg/ml）或明胶（0.1%） 培养基 含FBS、生长添加剂、Penicillin、Streptomycin等 产品货号 CM-R133 换液频率 每2-3天换液一次 生长特性 贴壁 细胞形态 内皮细胞样 传代特性 可传2-3代 消化液 0.25%胰蛋白酶 大鼠海绵体内皮细胞体外培养周期有限；建议使用普诺赛配套的专用生长培养基及正确的操作方法来培养，以此保证该细胞的最佳培养状态。
参考文献
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3-Hydroxy-3-methylglutaryl-CoA synthase 2 facilitates erectile dysfunction via inhibiting autophagy by enhancing the mammalian target of rapamycin pathway in type 1 diabetic mellitus rats (2024-01-25)
作者：Zhiqiang Dai:1.Department of Urology, China and Sichuan Provincial Clinical Research Center for Kidney Diseases, the Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan Province, China. ; Yang Zeng:1.Department of Urology, China and Sichuan Provincial Clinical Research Center for Kidney Diseases, the Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan Province, China. ; Xiao Tan:1.Department of Urology, China and Sichuan Provincial Clinical Research Center for Kidney Diseases, the Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan Province, China. ; Tao Zhou:1.Department of Urology, China and Sichuan Provincial Clinical Research Center for Kidney Diseases, the Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan Province, China. ; Xiong Li:1.Department of Urology, China and Sichuan Provincial Clinical Research Center for Kidney Diseases, the Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan Provi
期刊：3-Hydroxy-3-methylglutaryl-CoA synthase 2 facilitates erectile dysfunction via inhibiting autophagy by enhancing the mammalian target of rapamycin pathway in type 1 diabetic mellitus rats
影响因子 ：4.5
引用产品： 大鼠海绵体内皮细胞
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Combining transcriptomic analysis and network pharmacology to explore the mechanism by which Shaofu Zhuyu decoction improves diabetes mellitus erectile dysfunction (2023-08-06)
作者：Yinhui Mao:1.Changchun University of Chinese Medicine, Changchun 130117, China. ; Juntao Sun:1.Changchun University of Chinese Medicine, Changchun 130117, China. ; Zhuo Wang:1.Changchun University of Chinese Medicine, Changchun 130117, China. ; Yang Liu:1.Changchun University of Chinese Medicine, Changchun 130117, China. ; Jilei Sun:1.Department of Urology, The Affiliated Hospital of Changchun University of Chinese Medicine, Changchun 130021, China. ; Zhitao Wei:1.Department of Urology, The Affiliated Hospital of Changchun University of Chinese Medicine, Changchun 130021, China. ; Mingxing Wang:1.The Affiliated Hospital of Changchun University of Chinese Medicine, Changchun 130021, China. Electronic address: cc_wmx@163.com. ; Yong Yang:1.Changchun University of Chinese Medicine, Changchun 130117, China; Department of Urology, The Affiliated Hospital of Changchun University of Chinese Medicine, Changchun 130021, China. Electronic address: yongyang301@163.com. ;
期刊：Combining transcriptomic analysis and network pharmacology to explore the mechanism by which Shaofu Zhuyu decoction improves diabetes mellitus erectile dysfunction
DOI：10.1016/j.phymed.2023.155006
影响因子 ：7.9
引用产品： 大鼠海绵体内皮细胞
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Transcriptome analysis highlights the role of ferroptosis in palmitic acid–induced endothelial dysfunction (2023-01-24)
作者：Xiao-Hui Tan:1.Department of Urology, Peking University First Hospital, Beijing 100034, PR China. ; 2.Institute of Urology, Peking University, Beijing 100034, PR China. ; 3.Beijing Key Laboratory of Urogenital Diseases (male) Molecular Diagnosis and Treatment Center, Beijing 100034, PR China. ; Yang-Yang Gu:1.Institute of Urology, Peking University, Beijing 100034, PR China. ; 2.Beijing Key Laboratory of Urogenital Diseases (male) Molecular Diagnosis and Treatment Center, Beijing 100034, PR China. ; 3.Department of Radiation Medicine, Institute of Systems Biomedicine, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, PR China. ; Wen-Peng Song:1.Institute of Urology, Peking University, Beijing 100034, PR China. ; 2.Beijing Key Laboratory of Urogenital Diseases (male) Molecular Diagnosis and Treatment Center, Beijing 100034, PR China. ; 3.Department of Stomatology, Beijing Tiantan Hospital, Capital Medical University, Beijing 100070, PR China. ;
期刊：Transcriptome analysis highlights the role of ferroptosis in palmitic acid–induced endothelial dysfunction
影响因子 ：2.6
引用产品： 大鼠海绵体内皮细胞
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Curcumin Attenuates Ferroptosis and Ameliorates Erectile Function in Diabetic Rats by Activating Nrf2/HO-1 Pathway (2023-11-15)
作者：Department of Andrology, Nanjing Drum Tower Hospital Clinical College of Nanjing University of Chinese Medicine, 321 Zhongshan Road, Nanjing, Jiangsu 210008, China
期刊：Curcumin Attenuates Ferroptosis and Ameliorates Erectile Function in Diabetic Rats by Activating Nrf2/HO-1 Pathway
影响因子 ：2.4
引用产品： 大鼠海绵体内皮细胞
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Elevated pigment epithelium-derived factor induces diabetic erectile dysfunction via interruption of the Akt/Hsp90β/eNOS complex (2005-00-20)
作者：Di Che:1.Program of Molecular Medicine, Affiliated Guangzhou Women and Children's Hospital, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China. ; 2.Department of Biochemistry, Zhongshan School of Medicine, Sun Yat-sen University, 74 Zhongshan Road II, Guangzhou, 510080, China. ; 3.Department of Clinical Biological Resource Bank, Guangzhou Institute of Pediatrics, Guangzhou Women and Children's Medical Center, Guangzhou, China. ; Zhenzhen Fang:1.Department of Biochemistry, Zhongshan School of Medicine, Sun Yat-sen University, 74 Zhongshan Road II, Guangzhou, 510080, China. ; Li Yan:1.Department of Endocrinology, Second Affiliated Hospital, Sun Yat-sen University, Guangzhou, China. ; Jieping Du:1.Department of Endocrinology, Second Affiliated Hospital, Sun Yat-sen University, Guangzhou, China. ; Fangping Li:1.Department of Endocrinology, Second Affiliated Hospital, Sun Yat-sen University, Guangzhou, China. ; Jinye Xie:1.Department of Biochemistry, Zhongshan Schoo
期刊：Elevated pigment epithelium-derived factor induces diabetic erectile dysfunction via interruption of the Akt/Hsp90β/eNOS complex
DOI：10.1007/s00125-020-05147-y
影响因子 ：8.2
引用产品： 大鼠海绵体内皮细胞
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Transplantation of Human Urine-Derived Stem Cells Ameliorates Erectile Function and Cavernosal Endothelial Function by Promoting Autophagy of Corpus Cavernosal Endothelial Cells in Diabetic Erectile Dysfunction Rats (2009-09-20)
作者：Chi Zhang:1.Department of Andrology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China. ; 2.Reproductive Medicine Research Center, The Sixth Affiliated Hospital of Sun Yat-sen University, Guangzhou 510000, China. ; Daosheng Luo:1.Department of Urology, Dongguan People's Hospital, Dongguan 523000, China. ; Tingting Li:1.Reproductive Medicine Research Center, The Sixth Affiliated Hospital of Sun Yat-sen University, Guangzhou 510000, China. ; Qiyun Yang:1.Department of Andrology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China. ; Yun Xie:1.Department of Andrology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China. ; 2.Guangdong Provincial Key Laboratory of Orthopedics and Traumatology, Guangzhou 510000, China. ; Haicheng Chen:1.Department of Andrology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China. ; Linyan Lv:1.Reproductive Medicine Research Center, The Sixth Affiliated Hospital of
期刊：Transplantation of Human Urine-Derived Stem Cells Ameliorates Erectile Function and Cavernosal Endothelial Function by Promoting Autophagy of Corpus Cavernosal Endothelial Cells in Diabetic Erectile Dysfunction Rats
影响因子 ：4.3
引用产品： 大鼠海绵体内皮细胞
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