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大鼠外周血来源内皮祖细胞
Rat Peripheral Blood-Derived Endothelial Progenitor Cells
货号：CP-R134
规格：5×10⁵Cells/T25培养瓶
价格： ￥4880
培养基货号： CM-R134
产品概述
大鼠外周血来源内皮祖细胞
Cat NO.: CP-R134
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产品名称：大鼠外周血来源内皮祖细胞
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组织来源：外周血
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产品规格：5×10⁵Cells/T25培养瓶
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细胞简介：
大鼠外周血来源内皮祖细胞分离自外周血；外周血是除骨髓之外的血液，临床上常用一些方法把骨髓中的造血干细胞释放到血液中，再在从血液中提取分离得到造血干细胞，我们把这样得到的干细胞称为外周血干细胞，在二十一世纪初人类开始的生命方舟计划中首次提出外周血这一新概念。内皮祖细胞是血管内皮细胞的前体细胞，亦称为成血管细胞(Angioblast)，是一群具有游走特性，能进一步增殖分化的幼稚内皮细胞，缺乏成熟内皮细胞的特征性表型，不能形成管腔样结构，其功能主要为参与了出生后缺血组织的血管发生和血管损伤后的修复。研究显示，内皮祖细胞在心脑血管疾病、外周血管疾病、肿瘤血管形成及创伤愈合等方面均发挥重要作用，并为缺血性疾病的研究治疗提供了新思路，EPCs生物学特性和治疗作用的研究成为这一领域新的热点。
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方法简介：
普诺赛实验室分离的大鼠外周血来源内皮祖细胞采用采集外周血、密度梯度离心、差速贴壁法结合内皮细胞专用培养基培养筛选制备而来，细胞总量约为5×10⁵cells/瓶。
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质量检测：
普诺赛实验室分离的大鼠外周血来源内皮祖细胞经CD34免疫荧光鉴定，纯度可达90%以上，且不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等。
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培养信息：
包被条件 PLL（0.1mg/ml）或明胶（0.1%） 培养基 含FBS、生长添加剂、Penicillin、Streptomycin等 产品货号 CM-R134 换液频率 每2-3天换液一次 生长特性 贴壁 细胞形态 内皮细胞样 传代特性 可传1-2代；不建议多次传代 消化液 0.25%胰蛋白酶 大鼠外周血来源内皮祖细胞体外培养周期有限；建议使用普诺赛配套的专用生长培养基及正确的操作方法来培养，以此保证该细胞的最佳培养状态。
参考文献
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Construction of tissue-engineered vascular grafts with enhanced patency by integrating heparin, cell-adhesive peptide, and carbon monoxide nanogenerators into acellular blood vessels (2023-12-28)
作者：Yonghong Fan:1.Department of Anatomy, Engineering Research Center for Organ Intelligent Biological Manufacturing of Chongqing, Key Lab for Biomechanics and Tissue Engineering of Chongqing, Third Military Medical University, Chongqing, 400038, China. ; 2.Laboratory of Basic Medicine, The General Hospital of Western Theater Command, Chengdu, 610083, China. ; 3.Engineering Research Center of Tissue and Organ Regeneration and Manufacturing, Ministry of Education, Chongqing, 400038, China. ; Juan Pei:1.Department of Anatomy, Engineering Research Center for Organ Intelligent Biological Manufacturing of Chongqing, Key Lab for Biomechanics and Tissue Engineering of Chongqing, Third Military Medical University, Chongqing, 400038, China. ; Yinhua Qin:1.Department of Anatomy, Engineering Research Center for Organ Intelligent Biological Manufacturing of Chongqing, Key Lab for Biomechanics and Tissue Engineering of Chongqing, Third Military Medical University, Chongqing, 400038, China. ; Huifang
期刊：Construction of tissue-engineered vascular grafts with enhanced patency by integrating heparin, cell-adhesive peptide, and carbon monoxide nanogenerators into acellular blood vessels
DOI：10.1016/j.bioactmat.2023.12.015
影响因子 ：18.9
引用产品： 大鼠外周血来源内皮祖细胞 , 大鼠外周血来源内皮祖细胞完全培养基
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Nano artificial periosteum PCL/Ta/ZnO accelerates repair of periosteum via antibacterial, promoting vascularization and osteogenesis (2023-09-11)
作者：Wenbin Liu:1.Department of Orthopaedics, The Third Xiangya Hospital, Central South University, 138 Tongzipo Road, Changsha 410008, China. ; Kai Zhang:1.Department of Orthopedic Surgery, National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, China. ; Jiangyu Nan:1.Department of Orthopedic Surgery, National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, China. ; Pengfei Lei:1.Department of Orthopedics, The First Affiliated Hospital, Medical College of Zhejiang University, Hangzhou, China; Hunan Engineering Research Center of Biomedical Metal and Ceramic Implants, Changsha, China. Electronic address: leipengfei@zju.edu.cn. ; Yan Sun:1.Department of Orthopedics, The First Affiliated Hospital, Medical College of Zhejiang University, Hangzhou, China; Hunan Engineering Research Center of Biomedical Metal and Ceramic Implants, Changsha, China. Electronic address: sunyan_n@zju.
期刊：Nano artificial periosteum PCL/Ta/ZnO accelerates repair of periosteum via antibacterial, promoting vascularization and osteogenesis
DOI：10.1016/j.bioadv.2023.213624
影响因子 ：0.0
引用产品： 大鼠骨髓来源内皮祖细胞
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Construction of tissue-engineered vascular grafts with high patency by mimicking immune stealth and blocking TGF-β mediated endothelial-to-mesenchymal transition (2022-12-25)
作者：Yonghong Fan: 1.Department of Anatomy, Third Military Medical University, Chongqing, 400038, China; Juan Pei: 1.State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, 650500, China; Xinxin Li: 1.State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, 650500, China; Yinhua Qin: 1.Department of Anatomy, Third Military Medical University, Chongqing, 400038, China; Youqian Xu: 1.Department of Anatomy, Third Military Medical University, Chongqing, 400038, China; Ming Ke: 1.Department of Anatomy, Third Military Medical University, Chongqing, 400038, China; Jie Zhang: 1.State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, 650500, China; Yong Liu: 1.Department of Anatomy, Third Military Medical Universit
期刊：Construction of tissue-engineered vascular grafts with high patency by mimicking immune stealth and blocking TGF-β mediated endothelial-to-mesenchymal transition
DOI：10.1016/j.compositesb.2022.110487
影响因子 ：11.3
引用产品： 大鼠外周血来源内皮祖细胞
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