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小鼠肺大动脉内皮细胞
Mouse Pulmonary Great Artery Endothelial Cells
货号：CP-M011
规格：5×10⁵Cells/T25培养瓶
价格： ￥4380
培养基货号： CM-M011
产品概述
小鼠肺大动脉内皮细胞
Cat NO.: CP-M011
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产品名称：小鼠肺大动脉内皮细胞
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组织来源：肺动脉组织
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产品规格：5×10⁵Cells/T25培养瓶
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细胞简介：
小鼠肺大动脉内皮细胞分离自肺大动脉组织；肺大动脉亦称肺动脉干。在呼吸空气的脊椎动物中，把静脉血由心脏导向肺脏的动脉。肺大动脉起于右心室，在主动脉之前向左上后方斜行，在主动脉弓下方分为左、右肺动脉，经肺门入肺。肺动脉干位于心包内，为一粗短的动脉干。起自右心室，在升主动脉前方向左后上方斜行，至主动脉弓下方分为左、右肺动脉。左肺动脉较短，在左主支气管前方横行，分二支进入左肺上、下叶。右肺动脉较长而粗，经升主动脉和上腔静脉后方向右横行，至右肺门处分为三支进入右肺上、中、下叶。细胞呈单层多角形铺路石状分布；该细胞在维持血管内外的动态平衡、合成和分泌细胞因子和介质、维持凝血和纤溶的动态平衡中起重要作用。肺大动脉内皮细胞呈单层多角形铺路石状分布，该细胞在维持血管内外的动态平衡、合成和分泌细胞因子和介质、维持凝血和纤溶的动态平衡中起重要作用。
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方法简介：
普诺赛实验室分离的小鼠肺大动脉内皮细胞采用胰蛋白酶-胶原酶联合消化法结合差速贴壁法、并通过内皮细胞专用培养基培养筛选制备而来，细胞总量约为5×10⁵cells/瓶。
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质量检测：
普诺赛实验室分离的小鼠肺大动脉内皮细胞经CD31免疫荧光鉴定，纯度可达90%以上，且不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等。
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培养信息：
包被条件 PLL（0.1mg/ml）或明胶（0.1%） 培养基 含FBS、生长添加剂、Penicillin、Streptomycin等 产品货号 CM-M011 换液频率 每2-3天换液一次 生长特性 贴壁 细胞形态 内皮细胞样 传代特性 可传2-3代 消化液 0.25%胰蛋白酶 小鼠肺大动脉内皮细胞体外培养周期有限；建议使用普诺赛配套的专用生长培养基及正确的操作方法来培养，以此保证该细胞的最佳培养状态。
参考文献
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CD137 Signaling Mediates Pulmonary Artery Endothelial Cell Proliferation Under Hypoxia By Regulating Mitochondrial Dynamics (2024-02-12)
作者：Hao Xia:1.Department of Cardiology, Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu, China. ; Junying Duan:1.Department of Cardiology, Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu, China. ; Mei Li:1.Department of Pathology, Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu, China. ; Nan Chen:1.Department of Cardiology, Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu, China. ; Wei Zhong:1.Department of Cardiology, Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu, China. ; Ye Zhou:1.Department of Cardiology, Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu, China. ; Rui Chen:1.Department of Cardiology, Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu, China. chenruicardiology@outlook.com. ; Wei Yuan:1.Department of Cardiology, Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu, China. yuanwei1@medmail.com.cn. ;
期刊：CD137 Signaling Mediates Pulmonary Artery Endothelial Cell Proliferation Under Hypoxia By Regulating Mitochondrial Dynamics
DOI：10.1007/s12265-024-10493-y
影响因子 ：3.4
引用产品： 小鼠肺大动脉内皮细胞
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Super Enhancer-Associated Circular RNA-CircKrt4 Regulates Hypoxic Pulmonary Artery Endothelial Cell Dysfunction in Mice (2023-05-04)
作者：Cui Ma:1.Central Laboratory of Harbin Medical University (Daqing), PR China (C.M., X.W., L.Z., X. Zhu, J.B., S.H., J.M., X.G., D.Z.). ; 2.College of Medical Laboratory Science and Technology (C.M., L.Z., X. Zhu, J.M., X. Zheng), Harbin Medical University (Daqing), PR China. ; Xiaoying Wang:1.Central Laboratory of Harbin Medical University (Daqing), PR China (C.M., X.W., L.Z., X. Zhu, J.B., S.H., J.M., X.G., D.Z.). ; 2.College of Pharmacy, Harbin Medical University, PR China (X.W., J.B., S.H., X.G., D.Z.). ; Lixin Zhang:1.Central Laboratory of Harbin Medical University (Daqing), PR China (C.M., X.W., L.Z., X. Zhu, J.B., S.H., J.M., X.G., D.Z.). ; 2.College of Medical Laboratory Science and Technology (C.M., L.Z., X. Zhu, J.M., X. Zheng), Harbin Medical University (Daqing), PR China. ; Xiangrui Zhu:1.Central Laboratory of Harbin Medical University (Daqing), PR China (C.M., X.W., L.Z., X. Zhu, J.B., S.H., J.M., X.G., D.Z.). ; 2.College of Medical Laboratory Science and Technology (C.M.
期刊：Super Enhancer-Associated Circular RNA-CircKrt4 Regulates Hypoxic Pulmonary Artery Endothelial Cell Dysfunction in Mice
DOI：10.1161/ATVBAHA.122.318842
影响因子 ：8.7
引用产品： 小鼠肺大动脉内皮细胞
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FUNDC1/USP15/Drp1 ameliorated TNF-α–induced pulmonary artery endothelial cell proliferation by regulating mitochondrial dynamics (2023-10-21)
作者：Chong Xu:1.Department of Cardiology, Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu, China; Department of Cardiology, Yancheng Third People's Hospital, Yancheng, Jiangsu, China. ; Bin Wang:1.Department of Geriatrics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. ; Mei Li:1.Department of Pathology, Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu, China. ; ZhiFeng Dong:1.Department of Cardiology, Yancheng Third People's Hospital, Yancheng, Jiangsu, China. ; Nan Chen:1.Department of Cardiology, Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu, China. ; Junying Duan:1.Department of Cardiology, Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu, China. ; Ye Zhou:1.Department of Cardiology, Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu, China. ; Mingfeng Jin:1.Department of Cardiology, Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu, China. ; Rui C
期刊：FUNDC1/USP15/Drp1 ameliorated TNF-α–induced pulmonary artery endothelial cell proliferation by regulating mitochondrial dynamics
DOI：10.1016/j.cellsig.2023.110939
影响因子 ：4.8
引用产品： 小鼠肺大动脉内皮细胞
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Long noncoding RNA Hoxaas3 contributes to hypoxia-induced pulmonary artery smooth muscle cell proliferation (2003-09-20)
作者：Hongyue Zhang:1.College of Pharmacy, Harbin Medical University, No.157, Baojian Road, Harbin, Heilongjiang, China. ; 2.Central Laboratory of Harbin Medical University-Daqing, No.38, Xinyang Road, Daqing, Heilongjiang, China. ; Ying Liu:1.College of Pharmacy, Harbin Medical University, No.157, Baojian Road, Harbin, Heilongjiang, China. ; 2.Central Laboratory of Harbin Medical University-Daqing, No.38, Xinyang Road, Daqing, Heilongjiang, China. ; Lixin Yan:1.College of Pharmacy, Harbin Medical University, No.157, Baojian Road, Harbin, Heilongjiang, China. ; 2.Central Laboratory of Harbin Medical University-Daqing, No.38, Xinyang Road, Daqing, Heilongjiang, China. ; Siqi Wang:1.College of Pharmacy, Harbin University of Commerce, No.138, Tongda Road, Harbin, Heilongjiang, China. ; Min Zhang:1.College of Pharmacy, Harbin Medical University, No.157, Baojian Road, Harbin, Heilongjiang, China. ; 2.Central Laboratory of Harbin Medical University-Daqing, No.38, Xinyang Road, Daqing, Heilongj
期刊：Long noncoding RNA Hoxaas3 contributes to hypoxia-induced pulmonary artery smooth muscle cell proliferation
影响因子 ：10.8
引用产品： 小鼠肺大动脉平滑肌细胞 , 小鼠肺大动脉内皮细胞
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