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小鼠心脏微血管内皮细胞
Mouse Cardiac Microvascular Endothelial Cells
货号：CP-M129
规格：5×10⁵Cells/T25培养瓶
价格： ￥4380
培养基货号： CM-M129
产品概述
小鼠心脏微血管内皮细胞
Cat NO.: CP-M129
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产品名称：小鼠心脏微血管内皮细胞
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英文简称：CMEC,CMECs
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组织来源：心脏组织
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产品规格：5×10⁵Cells/T25培养瓶
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细胞简介：
小鼠心脏微血管内皮细胞分离自心脏组织；心脏是脊椎动物身体中最重要的一个器官，主要功能是为血液流动提供压力，把血液运行至身体各个部分。心脏由心肌构成，左心房、左心室、右心房、右心室四个腔组成。左右心房之间和左右心室之间均由间隔隔开，故互不相通，心房与心室之间有瓣膜(房室瓣)，这些瓣膜使血液只能由心房流入心室，而不能倒流。心脏的作用是推动血液流动，向器官、组织提供充足的血流量，以供应氧和各种营养物质，并带走代谢的终产物(如二氧化碳、无机盐、尿素和尿酸等)，使细胞维持正常的代谢和功能。心脏微血管内皮细胞是组成心脏微血管腔面单层扁平上皮样细胞，它所产生和分泌的生物活性物质对维持血管张力、调节血压、抗血栓形成等有重要作用，在心脏血管疾病的发病机制中有重要病理生理学意义。近年来大量研究表明，心肌微血管内皮细胞的功能和病理改变直接影响心肌细胞功能，也是诸多毒素、炎症因子及病毒等重要靶位，其作为体外研究的细胞模型在心肌缺血- 再灌注发病机制和细胞间旁分泌细胞生长因子研究中起着重要作用。
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方法简介：
普诺赛实验室分离的小鼠心脏微血管内皮细胞采用胶原酶-中性蛋白酶混合消化法结合密度梯度离心法、最后通过内皮细胞专用培养基培养筛选制备而来，细胞总量约为5×10⁵cells/瓶。
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质量检测：
普诺赛实验室分离的小鼠心脏微血管内皮细胞经CD31免疫荧光鉴定，纯度可达90%以上，且不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等。
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培养信息：
包被条件 PLL（0.1mg/ml）或明胶（0.1%） 培养基 含FBS、生长添加剂、Penicillin、Streptomycin等 产品货号 CM-M129 换液频率 每2-3天换液一次 生长特性 贴壁 细胞形态 内皮细胞样 传代特性 可传2-3代 消化液 0.25%胰蛋白酶 小鼠心脏微血管内皮细胞体外培养周期有限；建议使用普诺赛配套的专用生长培养基及正确的操作方法来培养，以此保证该细胞的最佳培养状态。
参考文献
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Paracrine effects of mir-210-3p on angiogenesis in hypoxia-treated c-kit-positive cardiac cells (2023-07-22)
作者：Louyi Shen:1.Department of Cardiology, The First Affiliated Hospital of Guangxi Medical University, Nanning, China. ; 2.Guangxi Key Laboratory Base of Precision Medicine in Cardiocerebrovascular Diseases Control and Prevention, Nanning, China. ; 3.Guangxi Clinical Research Center for Cardiocerebrovascular Diseases, Nanning, China. ; Guan Fan:1.Department of Cardiology, The First Affiliated Hospital of Guangxi Medical University, Nanning, China. ; 2.Guangxi Key Laboratory Base of Precision Medicine in Cardiocerebrovascular Diseases Control and Prevention, Nanning, China. ; 3.Guangxi Clinical Research Center for Cardiocerebrovascular Diseases, Nanning, China. ; Guoliang Yang:1.Department of Cardiology, The First Affiliated Hospital of Guangxi Medical University, Nanning, China. ; 2.Guangxi Key Laboratory Base of Precision Medicine in Cardiocerebrovascular Diseases Control and Prevention, Nanning, China. ; 3.Guangxi Clinical Research Center for Cardiocerebrovascular Diseases, Nanning, C
期刊：Paracrine effects of mir-210-3p on angiogenesis in hypoxia-treated c-kit-positive cardiac cells
DOI：10.1080/07853890.2023.2237690
影响因子 ：4.4
引用产品： 小鼠心脏微血管内皮细胞
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Protective effects of melatonin on myocardial microvascular endothelial cell injury under hypertensive state by regulating Mst1 (2023-04-01)
作者：Lingpeng Wang:1.Department of Cardiology, the First Affiliated Hospital, Xinjiang Medical University, Urumqi, 830000, China. ; Wei Wang:1.Department of Internal Medicine, The First Affiliated Hospital, Xinjiang Medical University, No. 137, Liyushan South Road, Urumqi, Xinjiang, 830000, China. ; Ruimei Han:1.Department of Cardiology, Shanghai Xuhui Central Hospital, Shanghai, 200031, China. ; Yang Liu:1.Department of Internal Medicine, The First Affiliated Hospital, Xinjiang Medical University, No. 137, Liyushan South Road, Urumqi, Xinjiang, 830000, China. ; Bin Wu:1.Department of Geriatrics, Xinjiang Military General Hospital, 359 Youhao North Street, Urumqi, Xinjiang, 830000, China. wubin0917@163.com. ; Jian Luo:1.Department of Internal Medicine, The First Affiliated Hospital, Xinjiang Medical University, No. 137, Liyushan South Road, Urumqi, Xinjiang, 830000, China. docsummer@163.com. ;
期刊：Protective effects of melatonin on myocardial microvascular endothelial cell injury under hypertensive state by regulating Mst1
DOI：10.1186/s12872-023-03159-1
影响因子 ：2.1
引用产品： 小鼠心脏微血管内皮细胞 , 小鼠心脏微血管内皮细胞完全培养基
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TLN1 synergizes with ITGA5 to ameliorate cardiac microvascular endothelial cell dysfunction (2023-04-20)
作者：Xianfeng Wang:1.Emergency Department, Qinghai Cardio-Cerebrovascular Specialty Hospital, Qinghai High Altitude Medical Research Institute, Xining, Qinghai, P.R. China. ; Wenkai Mao:1.Medical Department, Qinghai Cardio-Cerebrovascular Specialty Hospital, Qinghai High Altitude Medical Research Institute, Xining, Qinghai, P.R. China. ; Xiaofeng Ma:1.Personnel Department, Qinghai Cardio-Cerebrovascular Specialty Hospital, Qinghai High Altitude Medical Research Institute, Xining, Qinghai, P.R. China. Maxiaofeng921113@163.com. ;
期刊：TLN1 synergizes with ITGA5 to ameliorate cardiac microvascular endothelial cell dysfunction
影响因子 ：1.2
引用产品： 小鼠心脏微血管内皮细胞
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miR‑223‑3p reduces high glucose and high fat‑induced endothelial cell injury in diabetic mice by regulating NLRP3 expression (2006-00-20)
作者：Bo Deng:1.Department of Endocrinology, The Third Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330008, P.R. China. ; Ying Hu:1.Department of Endocrinology, The Third Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330008, P.R. China. ; Xia Sheng:1.Department of Endocrinology, The Third Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330008, P.R. China. ; Huijun Zeng:1.Department of Pharmacy, The Third Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330008, P.R. China. ; Yanan Huo:1.Department of Endocrinology, Jiangxi Provincial People's Hospital, Nanchang, Jiangxi 330006, P.R. China. ;
期刊：miR‑223‑3p reduces high glucose and high fat‑induced endothelial cell injury in diabetic mice by regulating NLRP3 expression
影响因子 ：2.7
引用产品： 小鼠心脏微血管内皮细胞
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