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TNFSF2 (N-6His), Human, Recombinant
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货号：PCK052

价格：¥118034801288018880

规格：

10µg

10µg
50µg
500µg
1mg

数量： - +

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产品概述

产品信息

别名	Tumor Necrosis Factor; Cachectin; TNF-Alpha; Tumor Necrosis Factor Ligand Superfamily Member 2; TNF-a; TNF; TNFA; TNFSF2
物种	Human
表达宿主	E.coli
序列信息	Gly57-Leu233
检索号	P01375
分子量	21.8 kDa
表观分子量	18 kDa
标签	N-6His
生物活性	Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 30-150 pg/ml.

产品特性

纯度	>95% as determined by reducing SDS-PAGE.
内毒素	<1.0 EU per µg as determined by LAL test.
保存	Lyophilized protein should be stored at -5~-20℃, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at -5~-20℃ for 3 months.
运输	Ambient temperature or ice pack.
制剂	Lyophilized from a 0.2 μm filtered solution of 20mM PB,100mM NaCl, pH 7.2.
复融	Always centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

背景介绍

Tumor Necrosis Factor-α (TNF-α) is secreted by macrophages, monocytes, neutrophils, T-cells, and NK-cells following stimulation by bacterial LPS. Cells expressing CD4 secrete TNF-α while cells that express CD8 secrete little or no TNF-α. Synthesis of TNF-α can be induced by many different stimuli including interferons, IL2, and GM-CSF. The clinical use of the potent anti-tumor activity of TNF-α has been limited by the proinflammatory side effects such as fever, dose-limiting hypotension, hepatotoxicity, intravascular thrombosis, and hemorrhage. Designing clinically applicable TNF-α mutants with low systemic toxicity has been of intense pharmacological interest. Human TNF-α that binds to murine TNF-R55 but not murine TNF-R7, exhibits retained anti-tumor activity and reduced systemic toxicity in mice compared with murine TNF-α, which binds to both murine TNF Receptors. Based on these results, many TNF-α mutants that selectively bind to TNF-R55 have been designed. These mutants displayed cytotoxic activities on tumor cell lines in vitro and have exhibited lower systemic toxicity in vivo. Recombinant Human TNF-α High Active Mutant differs from the wild-type by amino acid subsitution of amino acids 1-7 with Arg8, Lys9, Arg10 and Phe157. This mutant form has been shown to have increased activity with less inflammatory side effects in vivo.

参考文献

文献列表

A microfluidic platform integrating dynamic cell culture and dielectrophoretic manipulation for in situ assessment of endothelial cell mechanics (2023-06-30)

作者：Hao Yang:1.Robotics and Microsystems Center, College of Mechanical and Electrical Engineering, Soochow University, Suzhou 215000, China. yhao@suda.edu.cn. ; Tao Chen:1.Robotics and Microsystems Center, College of Mechanical and Electrical Engineering, Soochow University, Suzhou 215000, China. yhao@suda.edu.cn. ; Yichong Hu:1.Robotics and Microsystems Center, College of Mechanical and Electrical Engineering, Soochow University, Suzhou 215000, China. yhao@suda.edu.cn. ; Fuzhou Niu:1.School of Mechanical Engineering, Suzhou University of Science and Technology, Suzhou 215000, China. ; Xinyu Zheng:1.Suzhou Medical College of Soochow University, Suzhou, Jiangsu, China. ; Haizhen Sun:1.Robotics and Microsystems Center, College of Mechanical and Electrical Engineering, Soochow University, Suzhou 215000, China. yhao@suda.edu.cn. ; Liang Cheng:1.Institute of Functional Nano & Soft Materials (FUNSOM), Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University

期刊：A microfluidic platform integrating dynamic cell culture and dielectrophoretic manipulation for in situ assessment of endothelial cell mechanics

DOI：10.1039/D3LC00363A

影响因子：6.1

引用产品：人脐静脉内皮细胞 , TNF-α/ TNFA/ TNFSF2, Human, Recombinant , Calcein-AM/PI活死细胞双染试剂盒