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产品概述
产品信息
| 别名 | Interleukin-2; IL-2; T-cell Growth Factor; TCGF; Aldesleukin |
| 物种 | Human |
| 表达宿主 | E.coli |
| 序列信息 | Pro22-Thr153(Cys145Ser) |
| 检索号 | P60568 |
| 分子量 | 15.5 kDa |
| 表观分子量 | 14 kDa |
| 生物活性 | Measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells.The ED50 for this effect is 90-270 pg/ml. |
产品特性
| 纯度 | >95% as determined by reducing SDS-PAGE. |
| 内毒素 | <1.0 EU per µg as determined by LAL test. |
| 保存 | Lyophilized protein should be stored at -5~-20℃, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at -5~-20℃ for 3 months. |
| 运输 | Ambient temperature or ice pack. |
| 制剂 | Lyophilized from a 0.2 μm filtered solution of 10mM Acetata-Na, 5% Trehaiose, pH 4.5. |
| 复融 | Always centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
背景介绍
Recombinant Human Interleukin-2 is a highly purified protein with a molecular weight of approximately 15,300 Daltons. The chemical name is des-alanyl-1, serine-145 Human Interleukin-2. It is produced by recombinant DNA technology using a genetically engineered E. coli strain containing an analog of the human interleukin-2 gene. Genetic engineering techniques were used to modify the Human IL-2 gene, and the resulting expression clone encodes a modified Human IL-2. This recombinant form differs from native Interleukin-2 in following ways: it is not glycosylated; the molecule has serine substituted for cysteine at amino acid position 145; the aggregation state of molecule is likely to be different from that of native IL-2.
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