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产品概述
产品信息
| 别名 | Erythropoietin; Epoetin; EPO |
| 物种 | Human |
| 表达宿主 | Human Cells |
| 序列信息 | Ala28-Arg193 |
| 检索号 | P01588 |
| 分子量 | 19.2 kDa |
| 表观分子量 | 30-40 kDa |
| 生物活性 | Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells.The ED50 for this effect is 80-250 pg/ml. |
产品特性
| 纯度 | >95% as determined by reducing SDS-PAGE. |
| 内毒素 | <1.0 EU per µg as determined by LAL test. |
| 保存 | Lyophilized protein should be stored at -5~-20℃, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at -5~-20℃ for 3 months. |
| 运输 | Ambient temperature or ice pack. |
| 制剂 | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
| 复融 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
背景介绍
Erythropoietin (EPO) is a glyco Protein hormone that is principally known for its role in erythropoiesis,where it is responsible for stimulating proliferation and differentiation of erythroid progenitor cells.Erythropoietin is a member of the EPO/ TPO family. It is a secreted, glycosylated Cytokine composed of fouralpha helical bundles. The differentiation of CFU-E (Colony Forming Unit-Erythroid) cells into erythrocytescan only be accomplished in the presence of EPO. Physiological levels of EPO in adult mammals aremaintained primarily by the kidneys, whereas levels in fetal or neonatal mammals are maintained by theliver. EPO also can exert various non-hematopoietic activities, including vascularization and proliferation ofsmooth muscle, neural protection during hypoxia, and stimulation of certain B cells. Genetic variation inerythropoietin is associated with susceptbility to microvascular complications of diabetes type 2. These arepathological conditions that develop in numerous tissues and organs as a consequence of diabetesmellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, anddiabetic neuropathy.
FAQs
Q：收到细胞因子产品用什么溶剂复融？
A：1、细胞因子产品大部分为冻干形式发货，少部分液体形式发货； 2、液体形式因子产品不需要复溶，具体浓度详见产品标签，按照实验需要用含载体蛋白(如0.1% BSA，10%FBS，5%HSA 或 5% 海藻糖)的溶液进一步稀释后分装冷冻保存即可； 3、冻干粉状态的因子产品需指定溶剂复溶，请参考产品说明书【复融】方法，按照要求使用对应溶剂溶解，部分需要无菌水以外的特殊溶剂溶解的产品则随货会赠送相应的复融试剂；冻干粉溶解后如您需要进一步稀释分装，需使用含载体蛋白(如0.1% BSA，10%FBS，5%HSA 或 5% 海藻糖)的溶液进一步稀释后分装冷冻保存。
Q：细胞因子离心前需要放置室温吗？
A：不用提前放置室温.
Q：细胞因子加无菌水溶解后需要再次离心吗？
A：加了无菌水后，如果没有挂壁现象是不用离心的，如果您放置了一段时间，再次使用时是可以使用离心机离心的
Q：细胞因子用无菌水配成了100ug/mL母液，用于实验时，需要的终浓度为10ng/mL，可以用培养基直接稀释？还是需要通过PBS（含5%海藻糖）进一步稀释才行呢？
A：如果是直接做实验，可以直接添加培养基进行稀释配置。 如果是需要长时间保存的话，需要使用含载体蛋白(如0.1%BSA，10% FBS，5%HSA或5%海藻糖)的溶液进一步释后分装保存于-5~-20℃或者-80℃冰箱
Q：官网的因子都是不含动物成分的么？
A：我们公司的因子都是重组的，是不含动物源成分的。
产品资料
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