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产品概述
| 名称 | SW948 (人结肠腺癌细胞) (STR鉴定正确) |
| 别称 | SW-948; SW 948 |
| 种属 | 人类 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 上皮细胞样 |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO 温度：液氮 |
| 培养方案A（默认） |
生长培养基:
培养条件:
气相：空气，100%, 温度：37℃
|
| 培养方案B（可选） |
培养条件:
气相：空气，95%；CO2，5%, 温度：37℃
|
| 推荐传代比例 | 1:2-1:3 |
| 推荐换液频率 | 2-3次/周 |
| 注意事项 | 1.该细胞推荐使用Leibovitz's L-15培养基进行培养，Leibovitz's L-15不可以通入二氧化碳，会产生细胞毒性；2.如您没有无二氧化碳的培养箱，可使用DMEM替代Leibovitz's L-15，使用DMEM培养基时即可正常通入5%二氧化碳；3.配套专用培养基默认Leibovitz's L-15配置，如需DMEM配方，请联系销售下单备注更改。 |
| 背景描述 | Established from the colon tumor of a 81-year-old Caucasian woman with colon adenocarcinoma (Duke C) |
| 年龄（性别） | 女性；81岁 |
| 组织来源 | 结肠 |
| 细胞类型 | 肿瘤细胞 |
| 肿瘤类型 | 结肠腺癌细胞 |
| 生物安全等级 | BSL-1 |
| 倍增时间 | ~120 hours,(PubMed=1000501); 2-4 d (DSMZ) |
| 致瘤性 | Yes, in nude miceTumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 107 cells. |
| 基因表达情况 | carcinoembryonic antigen (CEA) 7 ng/10^6 cells/10 days; colon specific antigen (CSAp) 750 units in 0.5 mL cell sonicate; keratin |
| 保藏机构 | ATCC; CCL-237DSMZ; ACC-326ECACC; 91030714 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 12 D2S1338 17,24 D3S1358 16 D5S818 11 D7S820 9,11 D8S1179 12,14 D13S317 10,11 D16S539 11,12 D18S51 19 D19S433 13,15 D21S11 25.2,29 FGA 24 PentaD 12 PentaE 13 TH01 6,9.3 TPOX 8,11 vWA 16,18 D6S1043 11,12 D12S391 16,18 D2S441 10,11 -
STR鉴定图
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参考文献
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c-Met-Targeting 19F MRI Nanoparticles with Ultralong Tumor Retention for Precisely Detecting Small or Ill-Defined Colorectal Liver Metastases (2023-04-25)
作者：Daoshuang Li:1.Department of Nuclear Medicine, the Fourth Hospital of Harbin Medical University, Harbin, Heilongjiang, People's Republic of China. ; 2.NHC Key Laboratory of Molecular Probe and Targeted Diagnosis and Therapy, Molecular Imaging Research Center (MIRC) of Harbin Medical University, Harbin, Heilongjiang, People's Republic of China. ; Jie Yang:1.Department of Nuclear Medicine, the Fourth Hospital of Harbin Medical University, Harbin, Heilongjiang, People's Republic of China. ; 2.NHC Key Laboratory of Molecular Probe and Targeted Diagnosis and Therapy, Molecular Imaging Research Center (MIRC) of Harbin Medical University, Harbin, Heilongjiang, People's Republic of China. ; Zuoyu Xu:1.Department of Nuclear Medicine, the Fourth Hospital of Harbin Medical University, Harbin, Heilongjiang, People's Republic of China. ; 2.NHC Key Laboratory of Molecular Probe and Targeted Diagnosis and Therapy, Molecular Imaging Research Center (MIRC) of Harbin Medical University, Harbin, Heilon
期刊：c-Met-Targeting 19F MRI Nanoparticles with Ultralong Tumor Retention for Precisely Detecting Small or Ill-Defined Colorectal Liver Metastases
影响因子 ：8.0
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MORC4 plays a tumor-promoting role in colorectal cancer via regulating PCGF1/CDKN1A axis in vitro and in vivo (2023-03-17)
作者：Yichao Liang:1.Department of General Surgery, Shengjing Hospital of China Medical University, Shenyang, 110004, Liaoning, China. ; Di Wu:1.Department of General Surgery, Shengjing Hospital of China Medical University, Shenyang, 110004, Liaoning, China. ; Qiao Qu:1.Department of General Surgery, Shengjing Hospital of China Medical University, Shenyang, 110004, Liaoning, China. ; Zhilong Li:1.Department of General Surgery, Shengjing Hospital of China Medical University, Shenyang, 110004, Liaoning, China. ; Hongzhuan Yin:1.Department of General Surgery, Shengjing Hospital of China Medical University, Shenyang, 110004, Liaoning, China. hzyin@cmu.edu.cn. ;
期刊：MORC4 plays a tumor-promoting role in colorectal cancer via regulating PCGF1/CDKN1A axis in vitro and in vivo
DOI：10.1038/s41417-023-00605-2
影响因子 ：6.4
引用产品： SW948 细胞
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Karyopherin Subunit Alpha 1 Enhances the Malignant Behaviors of Colon Cancer Cells via Promoting Nuclear Factor-κB p65 Nuclear Translocation (2023-04-10)
作者：Lianrong Zhao:1.Department of Infectious Diseases, Shengjing Hospital of China Medical University, Shenyang, 110004, Liaoning, China. ; Di Wu:1.Department of General Surgery, Shengjing Hospital of China Medical University, No. 36, Sanhao Street, Heping District, Shenyang, 110004, Liaoning, China. ; Qiao Qu:1.Department of General Surgery, Shengjing Hospital of China Medical University, No. 36, Sanhao Street, Heping District, Shenyang, 110004, Liaoning, China. ; Zhilong Li:1.Department of General Surgery, Shengjing Hospital of China Medical University, No. 36, Sanhao Street, Heping District, Shenyang, 110004, Liaoning, China. ; Hongzhuan Yin:1.Department of General Surgery, Shengjing Hospital of China Medical University, No. 36, Sanhao Street, Heping District, Shenyang, 110004, Liaoning, China. hzyin@cmu.edu.cn. ;
期刊：Karyopherin Subunit Alpha 1 Enhances the Malignant Behaviors of Colon Cancer Cells via Promoting Nuclear Factor-κB p65 Nuclear Translocation
DOI：10.1007/s10620-023-07936-y
影响因子 ：3.1
引用产品： SW948 细胞 , Leibovitz's L-15 培养基
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![NCI-H2452 [H2452] (人间皮瘤细胞) (STR鉴定正确)](/Public/upfile/pic/xbx/CL-0398-1.jpg)
