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产品概述
名称 | 293 [HEK-293] (人胚肾细胞) (STR鉴定正确) |
别称 | Hek293; HEK-293; HEK 293; HEK:293; 293; 293 HEK; Human Embryonic Kidney 293 |
种属 | 人 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养方案A(默认) |
生长培养基:
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
|
推荐传代比例 | 1:3-1:4 |
推荐换液频率 | 2-3次/周 |
注意事项 | 该细胞贴壁松散,操作时请尽量轻柔;换液时需预热培养基;收货如有大块脱落的细胞团,为正常现象,请按照收货注意事项处理。 |
背景描述 | 293 [HEK-293]细胞是剪切过的人腺病毒5(Ad5)转染的人胚肾细胞形成的永生化细胞,293 [HEK-293]细胞包含并表达转染的Ad5基因。早期报道中指出,293 [HEK-293]细胞基因组中含有腺病毒5(Ad5)基因组的左侧端和右侧端的DNA,但是现在明确了只存在其左侧端的DNA。经过对Ad5的插入点的克隆测序发现,Ad5的1-4344位线性核苷酸整合入293 [HEK-293]细胞19号染色体(19q13.2)。293 [HEK-293]细胞为人类腺病毒载体扩增的宿主,可表达异常的玻连蛋白的细胞表面受体,由整合素β1亚单位和玻连蛋白受体α-v亚单位组成。 |
年龄(性别) | 胎儿 |
组织来源 | 肾;以腺病毒5DNA进行转化 |
细胞类型 | 转化细胞系 |
生物安全等级 | BSL-2 |
倍增时间 | ~24-30 hours |
致瘤性 | Yes, forms tumors in nude mice. |
受体表达情况 | vitronectin |
保藏机构 | ATCC; CRL-1573 ATCC: PTA-4488 DSMZ: ACC-305 ECACC: 85120602 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 12 D2S1338 19 D3S1358 15,17 D5S818 8 D7S820 11,12 D8S1179 12,14 D13S317 12,14 D16S539 9,13 D18S51 17,18 D19S433 15,18 D21S11 28,30.2 FGA 23 PentaD 9,10 PentaE 7,15 TH01 7,9.3 TPOX 11 vWA 16,19 D6S1043 11 D12S391 19,21 D2S441 11,15 -
STR鉴定图
参考文献
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Landscape of pathogenic mutations in premature ovarian insufficiency(2023/02/02)
作者:Hanni Ke, Shuyan Tang, Ting Guo
期刊:NATURE MEDICINE
DOI:10.1038/s41591-022-02194-3
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Simulation guided intramolecular orthogonal reporters for dissecting cellular oxidative stress and response(2022/08/05)
作者:Yecan Pan, Rui Weng, Linghao Zhang
期刊:Nano Today
DOI:10.1016/j.nantod.2022.101573
影响因子 :17.400
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ANGPTL8 negatively regulates NF-κB activation byfacilitating selective autophagic degradation ofIKKγ(2017/12/18)
作者:Yu Zhang, Xian Guo, Wanyao Yan
期刊:Nature Communications
DOI:10.1038/s41467-017-02355-w
影响因子 :16.600
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Gelatin-Encapsulated Tetrahedral DNA Nanostructure Enhances Cellular Internalization for Treating Noise-Induced Hearing Loss(2024/02/08)
作者:Ke Xu, Yiwei Du, Baoying Xu
期刊:Small
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作者:Yuandong Xu, Yuexiao Hu, Heng Xia
期刊:Advanced Healthcare Materials
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Rational synthesis of carbon dots with phosphate ester group for direct mapping of endogenous alkaline phosphatase and polarity monitoring in living cells(2023/02/28)
作者:Yanzhi Xing, Yulong Xu, Yanna Lin
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DOI:10.1016/j.jcis.2023.02.133
影响因子 :9.900
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The novel protein complex FAPα/ITGA5 is involved in the bone destruction of apical periodontitis(2023/11/17)
作者:Shuo Liu, QianYang Chen, Lili Wang
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DOI:10.1016/j.ijbiomac.2023.128200
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影响因子 :8.000
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作者:Dong Yang, Yu Fan, Mingrui Xiong
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影响因子 :7.700
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DOI:10.1021/acs.analchem.2c04852
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作者:Fan Zhang, Yuanyuan Zhang, Yasai Sun
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影响因子 :6.100
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影响因子 :5.763
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DOI:10.1152/ajpcell.00037.2018
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Migration of Human Renal Tubular Epithelial Cells in Response to Physiological Electric Signals(2021/09/14)
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DOI:10.1152/ajpcell.00249.2023
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Integrated miRNA and mRNA omics reveal dioscin suppresses migration and invasion via MEK/ERK and JNK signaling pathways in human endometrial carcinoma in vivo and in vitro(2022/12/09)
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Antibacterial and Antifungal Properties of a Novel Antimicrobial Peptide GK-19 and Its Application in Skin and Soft Tissue Infections Induced by MRSA or Candida albicans(2022/09/13)
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DOI:10.3390/pharmaceutics14091937
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影响因子 :5.314
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Characterization of the metabolites of Eucommiae Cortex in rats provides a further insight into its estrogen-like effective substances(2023/12/20)
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期刊:BIOORGANIC CHEMISTRY
DOI:10.1016/j.bioorg.2023.107052
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DOI:10.1080/21655979.2022.2029108
影响因子 :4.900
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Ubiquitin ligase Triad1 promotes neurite outgrowth by inhibiting MDM2-mediated ubiquitination of the neuroprotective factor pleiotropin(2022/08/31)
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NCK1-AS1 Increases Drug Resistance of Glioma Cells to Temozolomide by Modulating miR-137/TRIM24(2020/03/05)
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LINC00839 Regulates Proliferation, Migration, Invasion, Apoptosis and Glycolysis in Neuroblastoma Cells Through miR-338-3p/GLUT1 Axis(2022/12/19)
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Circ_0116061 regulated the proliferation, apoptosis, and inflammation of osteoarthritis chondrocytes through regulating the miR-200b-3p/SMURF2 axis(2021/4/13)
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期刊:Journal of Orthopaedic Surgery and Research
DOI:10.1186/s13018-021-02391-9
影响因子 :2.600
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IRF1 promotes the chondrogenesis of human adipose-derived stem cells through regulating HILPDA(2023/02/21)
作者:Yujun Zhao, Xiaotie Wang, Keke Nie
期刊:TISSUE & CELL
DOI:10.1016/j.tice.2023.102046
影响因子 :2.600
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Proinflammatory Bone Marrow Mesenchymal Stem Cell-Derived Exosomal miR-150-3p Suppresses Proinflammatory Polarization of Alveolar Macrophages in Sepsis by Targeting Inhibin Subunit Beta A(2023/10/11)
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期刊:JOURNAL OF INTERFERON AND CYTOKINE RESEARCH
影响因子 :2.300
引用产品:CL-0001
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Elevated histone deacetylase 10 expression promotes the progression of clear cell renal cell carcinoma by Notch-1-PTEN signaling axis(2024/05/11)
作者:Bin Zheng, Xue Jiang, Yaqing Liu
期刊:Discover Oncology
DOI:10.1007/s12672-024-01018-9
影响因子 :2.200
引用产品:CL-0001
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Long Non-Coding RNA CASC2 Overexpression Ameliorates Sepsis-Associated Acute Kidney Injury by Regulating MiR-545-3p/PPARA axis(2021/05/03)
作者:Qionghua Hu, Weiwei Zen, Ming Zhang
期刊:Journal of Surgical Research
影响因子 :2.200
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LPS-induced PTGS2 manipulates the inflammatory response through trophoblast invasion in preeclampsia via NF-κB pathway(2022/10/31)
作者:Ruolan Li, Jirong Xie, Wei Xu
期刊:Reproductive Biology
DOI:10.1016/j.repbio.2022.100696
影响因子 :2.100
引用产品:CL-0001
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