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产品概述
名称 | E0771 (小鼠髓样乳腺癌细胞) (STR鉴定正确) |
别称 | Eo771; E0771; EO 771 |
种属 | 小鼠 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
冻存条件 | 冻存液:55% 基础培养基+40% FBS+5% DMSO 温度:液氮 |
培养方案A(默认) |
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
|
推荐传代比例 | 1:4-1:6 |
推荐换液频率 | 2-3次/周 |
注意事项 | 1.细胞生长速度较快,请合理控制传代比例;2.该细胞换液极易收缩,需预热培养基;收货如有细胞收缩为正常现象,消化离心重新1:1传代。 |
背景描述 | EO771 is an epithelial-like cell that was isolated from the mammary gland of a patient with carcinoma. The cells display an elongated morphology. Depositor note: cells are genetically unstable in continuous culture. The EO771 mammary tumor was first reported in 1948 as a spontaneous mammary carcinoma arising in a C57Bl/6 mouse. |
年龄(性别) | 雌性 |
组织来源 | 乳房;乳腺 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 乳腺癌细胞 |
生物安全等级 | BSL-1 |
倍增时间 | 24 hours |
应用领域 | 该细胞系可用于分析调节乳腺癌转移的基因和/或可能减少免疫活性C57Bl/6小鼠自发转移的药物。 |
保藏机构 | ATCC; CRL-3461 |
STR鉴定
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STR位点信息
18-3 16,17,18 6-7 15,16 5-5 17,21 X-1 26,29 1-2 19,20,22 7-1 25.2,26.2 8-1 16 1-1 17,18,19 19-2 13 15-3 22.3,23.3,25.3 12-1 17,18 6-4 18,19,20 4-2 21.3,22.3,24.3 3-2 14,15 2-1 16,18 13-1 17.1,18.1
11-2 15.3,16.3 17-2 15,16 -
STR鉴定图
参考文献
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Ginsenoside Rh1, a novel casein kinase II subunit alpha (CK2α) inhibitor, retards metastasis via disrupting HHEX/CCL20 signaling cascade involved in tumor cell extravasation across endothelial barrier (2023-11-07)
作者:Weiwei Zheng:1.Jiangsu Key Laboratory for Pharmacology and Safety Evaluation of Chinese Materia Medica, School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China; Department of Biochemistry and Molecular Biology, School of Medicine & Holistic Integrative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China. ; Peiliang Shen:1.Jiangsu Key Laboratory for Pharmacology and Safety Evaluation of Chinese Materia Medica, School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China; Department of Biochemistry and Molecular Biology, School of Medicine & Holistic Integrative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China. ; Chang Yu:1.Department of Biochemistry and Molecular Biology, School of Medicine & Holistic Integrative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China. ; Yu Tang:1.Jiangsu Key Laboratory for Pharmacology and Safety Evaluation of Chinese Materia Medica, School of P
期刊:Ginsenoside Rh1, a novel casein kinase II subunit alpha (CK2α) inhibitor, retards metastasis via disrupting HHEX/CCL20 signaling cascade involved in tumor cell extravasation across endothelial barrier
DOI:10.1016/j.phrs.2023.106986
影响因子 :9.3
引用产品: E0771 细胞 , MDA-MB-231 细胞 , MS1 细胞
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Cyclic diguanylate analogues: Facile synthesis, STING binding mode and anti-tumor immunity delivered by cytidinyl/cationic lipid (2022-12-26)
作者:Xudong Sun:1.State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, 100191, China. ; Xiaotong Yu:1.State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, 100191, China. ; Yaqi Zhao:1.State Key Laboratory of Chemical Resource Engineering, Institute of Materia Medica, College of Science, Beijing University of Chemical Technology, Beijing, 100029, China. ; Lei Xing:1.State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, 100191, China. ; Luxin Na:1.State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, 100191, China. ; Zhuo Chen:1.State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, 100191, China. ; Zhangping Xiao:1.State Key Laboratory of Natural and Biomimetic Drugs, School
期刊:Cyclic diguanylate analogues: Facile synthesis, STING binding mode and anti-tumor immunity delivered by cytidinyl/cationic lipid
DOI:10.1016/j.ejmech.2022.115053
影响因子 :7.1
引用产品: E0771 细胞 , RAW 264.7 细胞
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