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产品概述
名称 | K7M2 wt [K7M2-WT] (小鼠骨肉瘤成骨细胞) (STR鉴定正确) |
别称 | K7M2 wt; K7M2 |
种属 | 小鼠 |
生长特性 | 贴壁细胞 |
细胞形态 | 成骨细胞样 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养方案A(默认) |
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
|
推荐传代比例 | 1:3-1:6 |
推荐换液频率 | 2-3次/周 |
背景描述 | K7M2 wt [K7M2-WT]细胞抗原表达:Ⅷ因子、整合唾液酸蛋白(BSP)、二聚糖、饰胶蛋白聚糖、绒毛蛋白。此外,骨唾液酸蛋白、二聚糖、饰胶蛋白聚糖和骨调素的表达显示K7M2 wt [K7M2-WT]细胞骨家族细胞特性。 |
年龄(性别) | 雌性;成年 |
组织来源 | 品系:BALB/c;器官:骨;疾病:骨肉瘤;来源转移灶:肺;细胞类型:成骨细胞 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 肉瘤细胞 |
生物安全等级 | BSL-1 |
倍增时间 | ~31 hours |
致瘤性 | Yes, forms tumors in BALB/c mice with spontaneous metastasis to the lungs in over 90% of mice inoculated. |
抗原表达情况 | CD31+ |
基因表达情况 | secreted phosphoprotein 1 (osteopontin); factor VIII |
保藏机构 | ATCC; CRL-2836 |
STR鉴定
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STR位点信息
18-3 18 6-7 12 5-5 14 X-1 25 1-2 17 7-1 29 8-1 13,14 1-1 16 19-2 13 15-3 22.3 12-1 17 6-4 18 4-2 21.3 3-2 12,13 2-1 16 13-1 16.2
11-2 18 17-2 16,17 -
STR鉴定图
参考文献
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A bone-targeting permeable nanomicelle acting as an icebreaker for enhancing chemotherapy of osteosarcoma (2024-03-29)
作者:Bowen Duan: 1.NMPA Key Laboratory for Research and Evaluation of Pharmaceutical Preparations and Excipients, Department of Pharmaceutics, China Pharmaceutical University 24 Tong Jia Xiang, Nanjing 210009, China; Hongting Liu: 1.NMPA Key Laboratory for Research and Evaluation of Pharmaceutical Preparations and Excipients, Department of Pharmaceutics, China Pharmaceutical University 24 Tong Jia Xiang, Nanjing 210009, China; Xiaoxuan Ji: 1.NMPA Key Laboratory for Research and Evaluation of Pharmaceutical Preparations and Excipients, Department of Pharmaceutics, China Pharmaceutical University 24 Tong Jia Xiang, Nanjing 210009, China; Jingwen Dong: 1.NMPA Key Laboratory for Research and Evaluation of Pharmaceutical Preparations and Excipients, Department of Pharmaceutics, China Pharmaceutical University 24 Tong Jia Xiang, Nanjing 210009, China; Xinping Luo: 1.NMPA Key Laboratory for Research and Evaluation of Pharmaceutical Preparations and Excipients, Department of Pharmaceutics, China Ph
期刊:A bone-targeting permeable nanomicelle acting as an icebreaker for enhancing chemotherapy of osteosarcoma
影响因子 :15.1
引用产品: K7M2 wt [K7M2-WT] 细胞
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Investigating the synergy of Shikonin and Valproic acid in inducing apoptosis of osteosarcoma cells via ROS-mediated EGR1 expression (2024-02-15)
作者:Zhuo Chen:1.The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, PR China; Huzhou Central Hospital, Affiliated Huzhou Hospital, Zhejiang University School of Medicine, Huzhou, Zhejiang, PR China. ; Feng-Feng Wu:1.Huzhou Central Hospital, Affiliated Huzhou Hospital, Zhejiang University School of Medicine, Huzhou, Zhejiang, PR China; The Fifth School of Clinical Medicine of Zhejiang Chinese Medical University, Huzhou, Zhejiang, PR China; The Affiliated Central Hospital, Huzhou University, Huzhou, Zhejiang, PR China; Huzhou Basic and Clinical Translation of Orthopaedics Key Laboratory, Huzhou, Zhejiang, PR China. ; Jing Li:1.School of Medicine, Huzhou University, Huzhou, Zhejiang, PR China. ; Jia-Bao Dong:1.The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, PR China; Huzhou Central Hospital, Affiliated Huzhou Hospital, Zhejiang University School of Medicine, Huzhou, Zhejiang, PR China. ; Hong-Yi He:1.Schoo
期刊:Investigating the synergy of Shikonin and Valproic acid in inducing apoptosis of osteosarcoma cells via ROS-mediated EGR1 expression
DOI:10.1016/j.phymed.2024.155459
影响因子 :7.9
引用产品: HOS 细胞 , K7M2 wt [K7M2-WT] 细胞 , DMEM高糖 培养基
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Injectable thermosensitive black phosphorus nanosheet- and doxorubicin-loaded hydrogel for synergistic bone tumor photothermal-chemotherapy and osteogenesis enhancement (2023-03-25)
作者:Shihuai Li:1.Orthopaedic Medical Center, The Second Hospital of Jilin University, Changchun 130041, People's Republic of China. ; Yunan Qing:1.Orthopaedic Medical Center, The Second Hospital of Jilin University, Changchun 130041, People's Republic of China. ; Yan Lou:1.Department of Nephropathy, The Second Hospital of Jilin University, Changchun 130041, People's Republic of China. ; Ruiyan Li:1.Orthopaedic Medical Center, The Second Hospital of Jilin University, Changchun 130041, People's Republic of China. ; Hao Wang:1.Orthopaedic Medical Center, The Second Hospital of Jilin University, Changchun 130041, People's Republic of China. ; Xingyue Wang:1.Orthopaedic Medical Center, The Second Hospital of Jilin University, Changchun 130041, People's Republic of China. ; Boda Ying:1.Orthopaedic Medical Center, The Second Hospital of Jilin University, Changchun 130041, People's Republic of China. ; Xiongfeng Tang:1.Orthopaedic Medical Center, The Second Hospital of Jilin University, Cha
期刊:Injectable thermosensitive black phosphorus nanosheet- and doxorubicin-loaded hydrogel for synergistic bone tumor photothermal-chemotherapy and osteogenesis enhancement
DOI:10.1016/j.ijbiomac.2023.124209
影响因子 :8.2
引用产品: MC3T3-E1 Subclone 24 细胞 , 人脐静脉内皮细胞 , NCTC clone 929 [L 细胞, L-929] 细胞 , K7M2 wt [K7M2-WT] 细胞
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Sulfatinib, a novel multi-targeted tyrosine kinase inhibitor of FGFR1, CSF1R, and VEGFR1–3, suppresses osteosarcoma proliferation and invasion via dual role in tumor cells and tumor microenvironment (2023-06-08)
作者:Song Liao:1.Medical School of Chinese PLA, Beijing, China. ; 2.Senior Department of Orthopedics, The Fourth Medical Center, Chinese PLA General Hospital, Beijing, China. ; Jianxiong Li:1.Medical School of Chinese PLA, Beijing, China. ; 2.Senior Department of Orthopedics, The Fourth Medical Center, Chinese PLA General Hospital, Beijing, China. ; Song Gao:1.Senior Department of Orthopedics, The Fourth Medical Center, Chinese PLA General Hospital, Beijing, China. ; Yuchen Han:1.Medical School of Chinese PLA, Beijing, China. ; 2.Senior Department of Orthopedics, The Fourth Medical Center, Chinese PLA General Hospital, Beijing, China. ; Xinli Han:1.Senior Department of Orthopedics, The Fourth Medical Center, Chinese PLA General Hospital, Beijing, China. ; Yanan Wu:1.Medical School of Chinese PLA, Beijing, China. ; 2.Senior Department of Orthopedics, The Fourth Medical Center, Chinese PLA General Hospital, Beijing, China. ; Jingyou Bi:1.Medical School of Chinese PLA, Beijing, China. ;
期刊:Sulfatinib, a novel multi-targeted tyrosine kinase inhibitor of FGFR1, CSF1R, and VEGFR1–3, suppresses osteosarcoma proliferation and invasion via dual role in tumor cells and tumor microenvironment
影响因子 :4.7
引用产品: MG-63 细胞 , U-2 OS [U2OS] 细胞 , HOS 细胞 , K7M2 wt [K7M2-WT] 细胞 , RAW 264.7 细胞
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LPS combined with CD47mAb enhances the anti‑osteosarcoma ability of macrophages (2023-03-28)
作者:Peng Yuan:1.Department of Orthopedics, Fuyang Hospital of Anhui Medical University, Fuyang, Anhui 236000, P.R. China. ; 2.Department of Orthopedics, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230000, P.R. China. ; Yukang Que:1.Department of Orthopedics, Fuyang Hospital of Anhui Medical University, Fuyang, Anhui 236000, P.R. China. ; Yulei Liu:1.Department of Orthopedics, Fuyang Hospital of Anhui Medical University, Fuyang, Anhui 236000, P.R. China. ; 2.Department of Orthopedics, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230000, P.R. China. ; Peng He:1.Department of Orthopedics, Fuyang Hospital of Anhui Medical University, Fuyang, Anhui 236000, P.R. China. ; Zehao Guo:1.Department of Orthopedics, Fuyang Hospital of Anhui Medical University, Fuyang, Anhui 236000, P.R. China. ; Yong Hu:1.Department of Orthopedics, Fuyang Hospital of Anhui Medical University, Fuyang, Anhui 236000, P.R. China. ; 2.Department of Orthopedics, The
期刊:LPS combined with CD47mAb enhances the anti‑osteosarcoma ability of macrophages
影响因子 :2.9
引用产品: RAW 264.7 细胞 , K7M2 wt [K7M2-WT] 细胞 , U-2 OS [U2OS] 细胞 , MG-63 细胞
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Nanoengineering a metal–organic framework for osteosarcoma chemo-immunotherapy by modulating indoleamine-2,3-dioxygenase and myeloid-derived suppressor cells (2005-02-20)
作者:Qingxin Fan:1.Hospital of Chengdu Office of People's Government of Tibetan Autonomous Region (Hospital.C.T.), Sichuan University, Chengdu, 610041, China. ; 2.State Key Laboratory of Biotherapy and Cancer Center, Collaborative Innovation Center for Biotherapy, Sichuan University, Chengdu, 610041, China. ; 3.Department of Orthopedics, Hospital of Chengdu Office of People's Government of TibetanAutonomous Region (Hospital.C.T.), Orthopedic Research Institute, West China Hospital, Sichuan University, Chengdu, 610041, China. ; Jing Zuo:1.Hospital of Chengdu Office of People's Government of Tibetan Autonomous Region (Hospital.C.T.), Sichuan University, Chengdu, 610041, China. ; 2.State Key Laboratory of Biotherapy and Cancer Center, Collaborative Innovation Center for Biotherapy, Sichuan University, Chengdu, 610041, China. ; Hailong Tian:1.Hospital of Chengdu Office of People's Government of Tibetan Autonomous Region (Hospital.C.T.), Sichuan University, Chengdu, 610041, China. ; 2.State Ke
期刊:Nanoengineering a metal–organic framework for osteosarcoma chemo-immunotherapy by modulating indoleamine-2,3-dioxygenase and myeloid-derived suppressor cells
DOI:10.1186/s13046-022-02372-8
影响因子 :11.3
引用产品: K7M2 wt [K7M2-WT] 细胞
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MerTK-mediated efferocytosis promotes immune tolerance and tumor progression in osteosarcoma through enhancing M2 polarization and PD-L1 expression (2001-02-20)
作者:Jinti Lin:1.Department of Orthopedics, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, PR China. ; 2.Orthopedics Research Institute, Zhejiang University, Hangzhou, PR China. ; Ankai Xu:1.Department of Orthopedics, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, PR China. ; 2.Orthopedics Research Institute, Zhejiang University, Hangzhou, PR China. ; Jiakang Jin:1.Department of Orthopedics, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, PR China. ; 2.Orthopedics Research Institute, Zhejiang University, Hangzhou, PR China. ; Man Zhang:1.Department of Orthopedics, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, PR China. ; 2.Orthopedics Research Institute, Zhejiang University, Hangzhou, PR China. ; Jianan Lou:1.Department of Orthopedics, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, PR China. ; 2.Orthopedi
期刊:MerTK-mediated efferocytosis promotes immune tolerance and tumor progression in osteosarcoma through enhancing M2 polarization and PD-L1 expression
DOI:10.1080/2162402X.2021.2024941
影响因子 :7.2
引用产品: RAW 264.7 细胞 , K7M2 wt [K7M2-WT] 细胞 , NIH/3T3 细胞 , 293T [HEK-293T] 细胞
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Pharmacodynamics and pharmacokinetics of PLGA-based doxorubicin-loaded implants for tumor therapy (2022-02-11)
作者:Peng He:1.Department of Orthopedics, First Affiliated Hospital of Anhui Medical University, Hefei, China. ; Shenglin Xu:1.Department of Orthopedics, First Affiliated Hospital of Anhui Medical University, Hefei, China. ; Zehao Guo:1.Department of Orthopedics, First Affiliated Hospital of Anhui Medical University, Hefei, China. ; Peng Yuan:1.Department of Orthopedics, Fuyang Hospital of Anhui Medical University, Fuyang, China. ; Yulei Liu:1.Department of Orthopedics, Fuyang Hospital of Anhui Medical University, Fuyang, China. ; Yu Chen:1.Department of Pharmacy, Anqing Medical College, Anqing, China. ; Tiantian Zhang:1.Laboratory of Pharmaceutical Research, Anhui Zhongren Science and Technology Co., Ltd, Hefei, China. ; Yukang Que:1.Department of Orthopedics, First Affiliated Hospital of Anhui Medical University, Hefei, China. ; Yong Hu:1.Department of Orthopedics, First Affiliated Hospital of Anhui Medical University, Hefei, China. ;
期刊:Pharmacodynamics and pharmacokinetics of PLGA-based doxorubicin-loaded implants for tumor therapy
DOI:10.1080/10717544.2022.2032878
影响因子 :6.0
引用产品: K7M2 wt [K7M2-WT] 细胞
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