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  • K7M2 wt [K7M2-WT] (小鼠骨肉瘤成骨细胞) (STR鉴定正确)
  • K7M2 wt [K7M2-WT] (小鼠骨肉瘤成骨细胞) (STR鉴定正确)
  • K7M2 wt [K7M2-WT] (小鼠骨肉瘤成骨细胞) (STR鉴定正确)
  • K7M2 wt [K7M2-WT] (小鼠骨肉瘤成骨细胞) (STR鉴定正确)
  • K7M2 wt [K7M2-WT] (小鼠骨肉瘤成骨细胞) (STR鉴定正确)
  • K7M2 wt [K7M2-WT] (小鼠骨肉瘤成骨细胞) (STR鉴定正确)
  • K7M2 wt [K7M2-WT] (小鼠骨肉瘤成骨细胞) (STR鉴定正确)

K7M2 wt [K7M2-WT] (小鼠骨肉瘤成骨细胞) (STR鉴定正确)

货号:CL-0371

规格:1×10⁶cells/T25培养瓶

价格: ¥1600

生长培养基:DMEM10% FBS1% P/S

数量: - +

  • K7M2 wt [K7M2-WT] (小鼠骨肉瘤成骨细胞) (STR鉴定正确)
  • K7M2 wt [K7M2-WT] (小鼠骨肉瘤成骨细胞) (STR鉴定正确)
  • K7M2 wt [K7M2-WT] (小鼠骨肉瘤成骨细胞) (STR鉴定正确)
  • K7M2 wt [K7M2-WT] (小鼠骨肉瘤成骨细胞) (STR鉴定正确)
  • K7M2 wt [K7M2-WT] (小鼠骨肉瘤成骨细胞) (STR鉴定正确)
  • K7M2 wt [K7M2-WT] (小鼠骨肉瘤成骨细胞) (STR鉴定正确)
  • K7M2 wt [K7M2-WT] (小鼠骨肉瘤成骨细胞) (STR鉴定正确)

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产品概述
产品信息
名称 K7M2 wt [K7M2-WT] (小鼠骨肉瘤成骨细胞) (STR鉴定正确)
别称 K7M2 wt; K7M2
种属 小鼠
生长特性 贴壁细胞
细胞形态 成骨细胞样
冻存条件 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮
培养方案A(默认)
生长培养基:
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
推荐传代比例 1:3-1:6
推荐换液频率 2-3次/周
参考资料(来源文献)
背景描述 K7M2 wt [K7M2-WT]细胞抗原表达:Ⅷ因子、整合唾液酸蛋白(BSP)、二聚糖、饰胶蛋白聚糖、绒毛蛋白。此外,骨唾液酸蛋白、二聚糖、饰胶蛋白聚糖和骨调素的表达显示K7M2 wt [K7M2-WT]细胞骨家族细胞特性。
年龄(性别) 雌性;成年
组织来源 品系:BALB/c;器官:骨;疾病:骨肉瘤;来源转移灶:肺;细胞类型:成骨细胞
细胞类型 肿瘤细胞
肿瘤类型 肉瘤细胞
生物安全等级 BSL-1
倍增时间 ~31 hours
致瘤性 Yes, forms tumors in BALB/c mice with spontaneous metastasis to the lungs in over 90% of mice inoculated.
抗原表达情况 CD31+
基因表达情况 secreted phosphoprotein 1 (osteopontin); factor VIII
保藏机构 ATCC; CRL-2836
STR鉴定
  • STR位点信息
    18-3 18
    6-7 12
    5-5 14
    X-1 25
    1-2 17
    7-1 29
    8-1 13,14
    1-1 16
    19-2 13
    15-3 22.3
    12-1 17
    6-4 18
    4-2 21.3
    3-2 12,13
    2-1 16
    13-1

    16.2

    11-2 18
    17-2 16,17
  • STR鉴定图
参考文献
文献列表
  • A bone-targeting permeable nanomicelle acting as an icebreaker for enhancing chemotherapy of osteosarcoma (2024-03-29)

    作者:Bowen Duan: 1.NMPA Key Laboratory for Research and Evaluation of Pharmaceutical Preparations and Excipients, Department of Pharmaceutics, China Pharmaceutical University 24 Tong Jia Xiang, Nanjing 210009, China; Hongting Liu: 1.NMPA Key Laboratory for Research and Evaluation of Pharmaceutical Preparations and Excipients, Department of Pharmaceutics, China Pharmaceutical University 24 Tong Jia Xiang, Nanjing 210009, China; Xiaoxuan Ji: 1.NMPA Key Laboratory for Research and Evaluation of Pharmaceutical Preparations and Excipients, Department of Pharmaceutics, China Pharmaceutical University 24 Tong Jia Xiang, Nanjing 210009, China; Jingwen Dong: 1.NMPA Key Laboratory for Research and Evaluation of Pharmaceutical Preparations and Excipients, Department of Pharmaceutics, China Pharmaceutical University 24 Tong Jia Xiang, Nanjing 210009, China; Xinping Luo: 1.NMPA Key Laboratory for Research and Evaluation of Pharmaceutical Preparations and Excipients, Department of Pharmaceutics, China Ph

    期刊:A bone-targeting permeable nanomicelle acting as an icebreaker for enhancing chemotherapy of osteosarcoma

    DOI:10.1016/j.cej.2024.150821

    影响因子 :15.1

    引用产品: K7M2 wt [K7M2-WT] 细胞

  • Investigating the synergy of Shikonin and Valproic acid in inducing apoptosis of osteosarcoma cells via ROS-mediated EGR1 expression (2024-02-15)

    作者:Zhuo Chen:1.The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, PR China; Huzhou Central Hospital, Affiliated Huzhou Hospital, Zhejiang University School of Medicine, Huzhou, Zhejiang, PR China. ; Feng-Feng Wu:1.Huzhou Central Hospital, Affiliated Huzhou Hospital, Zhejiang University School of Medicine, Huzhou, Zhejiang, PR China; The Fifth School of Clinical Medicine of Zhejiang Chinese Medical University, Huzhou, Zhejiang, PR China; The Affiliated Central Hospital, Huzhou University, Huzhou, Zhejiang, PR China; Huzhou Basic and Clinical Translation of Orthopaedics Key Laboratory, Huzhou, Zhejiang, PR China. ; Jing Li:1.School of Medicine, Huzhou University, Huzhou, Zhejiang, PR China. ; Jia-Bao Dong:1.The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, PR China; Huzhou Central Hospital, Affiliated Huzhou Hospital, Zhejiang University School of Medicine, Huzhou, Zhejiang, PR China. ; Hong-Yi He:1.Schoo

    期刊:Investigating the synergy of Shikonin and Valproic acid in inducing apoptosis of osteosarcoma cells via ROS-mediated EGR1 expression

    DOI:10.1016/j.phymed.2024.155459

    影响因子 :7.9

    引用产品: HOS 细胞 ,  K7M2 wt [K7M2-WT] 细胞 ,  DMEM高糖 培养基

  • Injectable thermosensitive black phosphorus nanosheet- and doxorubicin-loaded hydrogel for synergistic bone tumor photothermal-chemotherapy and osteogenesis enhancement (2023-03-25)

    作者:Shihuai Li:1.Orthopaedic Medical Center, The Second Hospital of Jilin University, Changchun 130041, People's Republic of China. ; Yunan Qing:1.Orthopaedic Medical Center, The Second Hospital of Jilin University, Changchun 130041, People's Republic of China. ; Yan Lou:1.Department of Nephropathy, The Second Hospital of Jilin University, Changchun 130041, People's Republic of China. ; Ruiyan Li:1.Orthopaedic Medical Center, The Second Hospital of Jilin University, Changchun 130041, People's Republic of China. ; Hao Wang:1.Orthopaedic Medical Center, The Second Hospital of Jilin University, Changchun 130041, People's Republic of China. ; Xingyue Wang:1.Orthopaedic Medical Center, The Second Hospital of Jilin University, Changchun 130041, People's Republic of China. ; Boda Ying:1.Orthopaedic Medical Center, The Second Hospital of Jilin University, Changchun 130041, People's Republic of China. ; Xiongfeng Tang:1.Orthopaedic Medical Center, The Second Hospital of Jilin University, Cha

    期刊:Injectable thermosensitive black phosphorus nanosheet- and doxorubicin-loaded hydrogel for synergistic bone tumor photothermal-chemotherapy and osteogenesis enhancement

    DOI:10.1016/j.ijbiomac.2023.124209

    影响因子 :8.2

    引用产品: MC3T3-E1 Subclone 24 细胞 ,  人脐静脉内皮细胞 ,  NCTC clone 929 [L 细胞, L-929] 细胞 ,  K7M2 wt [K7M2-WT] 细胞

  • Sulfatinib, a novel multi-targeted tyrosine kinase inhibitor of FGFR1, CSF1R, and VEGFR1–3, suppresses osteosarcoma proliferation and invasion via dual role in tumor cells and tumor microenvironment (2023-06-08)

    作者:Song Liao:1.Medical School of Chinese PLA, Beijing, China. ; 2.Senior Department of Orthopedics, The Fourth Medical Center, Chinese PLA General Hospital, Beijing, China. ; Jianxiong Li:1.Medical School of Chinese PLA, Beijing, China. ; 2.Senior Department of Orthopedics, The Fourth Medical Center, Chinese PLA General Hospital, Beijing, China. ; Song Gao:1.Senior Department of Orthopedics, The Fourth Medical Center, Chinese PLA General Hospital, Beijing, China. ; Yuchen Han:1.Medical School of Chinese PLA, Beijing, China. ; 2.Senior Department of Orthopedics, The Fourth Medical Center, Chinese PLA General Hospital, Beijing, China. ; Xinli Han:1.Senior Department of Orthopedics, The Fourth Medical Center, Chinese PLA General Hospital, Beijing, China. ; Yanan Wu:1.Medical School of Chinese PLA, Beijing, China. ; 2.Senior Department of Orthopedics, The Fourth Medical Center, Chinese PLA General Hospital, Beijing, China. ; Jingyou Bi:1.Medical School of Chinese PLA, Beijing, China. ;

    期刊:Sulfatinib, a novel multi-targeted tyrosine kinase inhibitor of FGFR1, CSF1R, and VEGFR1–3, suppresses osteosarcoma proliferation and invasion via dual role in tumor cells and tumor microenvironment

    DOI:10.3389/fonc.2023.1158857

    影响因子 :4.7

    引用产品: MG-63 细胞 ,  U-2 OS [U2OS] 细胞 ,  HOS 细胞 ,  K7M2 wt [K7M2-WT] 细胞 ,  RAW 264.7 细胞

  • LPS combined with CD47mAb enhances the anti‑osteosarcoma ability of macrophages (2023-03-28)

    作者:Peng Yuan:1.Department of Orthopedics, Fuyang Hospital of Anhui Medical University, Fuyang, Anhui 236000, P.R. China. ; 2.Department of Orthopedics, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230000, P.R. China. ; Yukang Que:1.Department of Orthopedics, Fuyang Hospital of Anhui Medical University, Fuyang, Anhui 236000, P.R. China. ; Yulei Liu:1.Department of Orthopedics, Fuyang Hospital of Anhui Medical University, Fuyang, Anhui 236000, P.R. China. ; 2.Department of Orthopedics, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230000, P.R. China. ; Peng He:1.Department of Orthopedics, Fuyang Hospital of Anhui Medical University, Fuyang, Anhui 236000, P.R. China. ; Zehao Guo:1.Department of Orthopedics, Fuyang Hospital of Anhui Medical University, Fuyang, Anhui 236000, P.R. China. ; Yong Hu:1.Department of Orthopedics, Fuyang Hospital of Anhui Medical University, Fuyang, Anhui 236000, P.R. China. ; 2.Department of Orthopedics, The

    期刊:LPS combined with CD47mAb enhances the anti‑osteosarcoma ability of macrophages

    DOI:10.3892/ol.2023.13777

    影响因子 :2.9

    引用产品: RAW 264.7 细胞 ,  K7M2 wt [K7M2-WT] 细胞 ,  U-2 OS [U2OS] 细胞 ,  MG-63 细胞

  • Nanoengineering a metal–organic framework for osteosarcoma chemo-immunotherapy by modulating indoleamine-2,3-dioxygenase and myeloid-derived suppressor cells (2005-02-20)

    作者:Qingxin Fan:1.Hospital of Chengdu Office of People's Government of Tibetan Autonomous Region (Hospital.C.T.), Sichuan University, Chengdu, 610041, China. ; 2.State Key Laboratory of Biotherapy and Cancer Center, Collaborative Innovation Center for Biotherapy, Sichuan University, Chengdu, 610041, China. ; 3.Department of Orthopedics, Hospital of Chengdu Office of People's Government of TibetanAutonomous Region (Hospital.C.T.), Orthopedic Research Institute, West China Hospital, Sichuan University, Chengdu, 610041, China. ; Jing Zuo:1.Hospital of Chengdu Office of People's Government of Tibetan Autonomous Region (Hospital.C.T.), Sichuan University, Chengdu, 610041, China. ; 2.State Key Laboratory of Biotherapy and Cancer Center, Collaborative Innovation Center for Biotherapy, Sichuan University, Chengdu, 610041, China. ; Hailong Tian:1.Hospital of Chengdu Office of People's Government of Tibetan Autonomous Region (Hospital.C.T.), Sichuan University, Chengdu, 610041, China. ; 2.State Ke

    期刊:Nanoengineering a metal–organic framework for osteosarcoma chemo-immunotherapy by modulating indoleamine-2,3-dioxygenase and myeloid-derived suppressor cells

    DOI:10.1186/s13046-022-02372-8

    影响因子 :11.3

    引用产品: K7M2 wt [K7M2-WT] 细胞

  • MerTK-mediated efferocytosis promotes immune tolerance and tumor progression in osteosarcoma through enhancing M2 polarization and PD-L1 expression (2001-02-20)

    作者:Jinti Lin:1.Department of Orthopedics, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, PR China. ; 2.Orthopedics Research Institute, Zhejiang University, Hangzhou, PR China. ; Ankai Xu:1.Department of Orthopedics, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, PR China. ; 2.Orthopedics Research Institute, Zhejiang University, Hangzhou, PR China. ; Jiakang Jin:1.Department of Orthopedics, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, PR China. ; 2.Orthopedics Research Institute, Zhejiang University, Hangzhou, PR China. ; Man Zhang:1.Department of Orthopedics, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, PR China. ; 2.Orthopedics Research Institute, Zhejiang University, Hangzhou, PR China. ; Jianan Lou:1.Department of Orthopedics, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, PR China. ; 2.Orthopedi

    期刊:MerTK-mediated efferocytosis promotes immune tolerance and tumor progression in osteosarcoma through enhancing M2 polarization and PD-L1 expression

    DOI:10.1080/2162402X.2021.2024941

    影响因子 :7.2

    引用产品: RAW 264.7 细胞 ,  K7M2 wt [K7M2-WT] 细胞 ,  NIH/3T3 细胞 ,  293T [HEK-293T] 细胞

  • Pharmacodynamics and pharmacokinetics of PLGA-based doxorubicin-loaded implants for tumor therapy (2022-02-11)

    作者:Peng He:1.Department of Orthopedics, First Affiliated Hospital of Anhui Medical University, Hefei, China. ; Shenglin Xu:1.Department of Orthopedics, First Affiliated Hospital of Anhui Medical University, Hefei, China. ; Zehao Guo:1.Department of Orthopedics, First Affiliated Hospital of Anhui Medical University, Hefei, China. ; Peng Yuan:1.Department of Orthopedics, Fuyang Hospital of Anhui Medical University, Fuyang, China. ; Yulei Liu:1.Department of Orthopedics, Fuyang Hospital of Anhui Medical University, Fuyang, China. ; Yu Chen:1.Department of Pharmacy, Anqing Medical College, Anqing, China. ; Tiantian Zhang:1.Laboratory of Pharmaceutical Research, Anhui Zhongren Science and Technology Co., Ltd, Hefei, China. ; Yukang Que:1.Department of Orthopedics, First Affiliated Hospital of Anhui Medical University, Hefei, China. ; Yong Hu:1.Department of Orthopedics, First Affiliated Hospital of Anhui Medical University, Hefei, China. ;

    期刊:Pharmacodynamics and pharmacokinetics of PLGA-based doxorubicin-loaded implants for tumor therapy

    DOI:10.1080/10717544.2022.2032878

    影响因子 :6.0

    引用产品: K7M2 wt [K7M2-WT] 细胞

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