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产品概述
名称 | RF/6A (猴脉络膜-视网膜内皮细胞) (种属鉴定正确) |
种属 | 猴 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养方案A(默认) |
生长培养基:
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
|
推荐传代比例 | 1:2-1:4 |
推荐换液频率 | 2-3次/周 |
背景描述 | RF/6A细胞在早期自发转化并已传代540次以上;电镜下观察到Weibel-Palade颗粒。高代次的RF/6A细胞用作滋养层以增强贴壁,转接能力和供养眼色素层黑素细胞。 |
年龄(性别) | 胚胎 |
组织来源 | 脉络膜;视网膜;内皮细胞;正常 |
细胞类型 | 自发永生化细胞 |
生物安全等级 | BSL-1 |
基因表达情况 | factor VIII; fibronectin |
保藏机构 | ATCC; CRL-1780 |
种属鉴定
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种属鉴定图
参考文献
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Syringaresinol Alleviates Early Diabetic Retinopathy by Downregulating HIF-1α/VEGF via Activating Nrf2 Antioxidant Pathway (2024-02-14)
作者:Chang Liu:1.Department of Molecular Pharmacology, School of Medicine, Nankai University Tianjin, Tianjin, 300071, China. ; 2.Nankai University Eye Institute, Tianjin, 300071, China. ; Tianwei Cheng:1.Department of Molecular Pharmacology, School of Medicine, Nankai University Tianjin, Tianjin, 300071, China. ; Yufei Wang:1.Department of Molecular Pharmacology, School of Medicine, Nankai University Tianjin, Tianjin, 300071, China. ; Guangru Li:1.Department of Molecular Pharmacology, School of Medicine, Nankai University Tianjin, Tianjin, 300071, China. ; Yachen Wang:1.Tianjin Key Lab of Ophthalmology and Visual Science, Tianjin Eye Hospital, Tianjin Eye Institute, Nankai University Affiliated Eye Hospital, Clinical College of Ophthalmology Tianjin Medical University, Tianjin, 300020, China. ; Wencong Tian:1.Department of General Surgery, Tianjin Union Medical Center, Tianjin, 300122, China. ; Lifeng Feng:1.Department of Molecular Pharmacology, School of Medicine, Nankai University
期刊:Syringaresinol Alleviates Early Diabetic Retinopathy by Downregulating HIF-1α/VEGF via Activating Nrf2 Antioxidant Pathway
影响因子 :5.2
引用产品: RF/6A 细胞 , RF/6A细胞专用培养基
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Fe-curcumin nanozyme-mediated immunosuppression and anti-inflammation in experimental autoimmune uveitis (2023-12-12)
作者:Zhengxuan Jiang:1.Department of Ophthalmology, The Second Affiliated Hospital, Anhui Medical University, Hefei, 230601, Anhui, P. R. China. ; Kun Liang:1.Department of Ophthalmology, The Second Affiliated Hospital, Anhui Medical University, Hefei, 230601, Anhui, P. R. China. ; Xiang Gao:1.Department of Ophthalmology, The Second Affiliated Hospital, Anhui Medical University, Hefei, 230601, Anhui, P. R. China. ; Fan Cao:1.Department of Ophthalmology, The Second Affiliated Hospital, Anhui Medical University, Hefei, 230601, Anhui, P. R. China. ; Guangqi An:1.The First Affiliated Hospital of Zhengzhou University, Academy of Medical Sciences of Zhengzhou University, Zhengzhou, 450052, Henan, P. R. China. ; Siyu Gui:1.Department of Ophthalmology, The Second Affiliated Hospital, Anhui Medical University, Hefei, 230601, Anhui, P. R. China. ; Weiwei Tang:1.Department of Ophthalmology, The Second Affiliated Hospital, Anhui Medical University, Hefei, 230601, Anhui, P. R. China. ; Liping Du:
期刊:Fe-curcumin nanozyme-mediated immunosuppression and anti-inflammation in experimental autoimmune uveitis
DOI:10.1186/s40824-023-00451-1
影响因子 :11.3
引用产品: DMEM高糖 培养基 , RF/6A细胞专用培养基 , 磷酸盐缓冲液 (PBS) 1× , RF/6A 细胞
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IL1R2 promotes retinal angiogenesis to participate in retinopathy of prematurity by activating the HIF1α/PFKFB3 pathway (2023-12-13)
作者:Na Zhou:1.Department of Ophthalmolog, Chenzhou First People's Hospital, 102 Luojiajing, Beihu District, Chenzhou, 423000, China. ; Li Liu:1.Department of Ophthalmolog, Chenzhou First People's Hospital, 102 Luojiajing, Beihu District, Chenzhou, 423000, China. ; Qiaolian Li:1.Department of Ophthalmolog, Chenzhou First People's Hospital, 102 Luojiajing, Beihu District, Chenzhou, 423000, China. Electronic address: 18173506927@163.com. ;
期刊:IL1R2 promotes retinal angiogenesis to participate in retinopathy of prematurity by activating the HIF1α/PFKFB3 pathway
DOI:10.1016/j.exer.2023.109750
影响因子 :3.4
引用产品: RF/6A 细胞
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Autophagy is required for the promoting effect of angiogenic factor with G patch domain and forkhead-associated domain 1 (AGGF1) in retinal angiogenesis (2007-01-20)
作者:Rong Li:1.Department of Ophthalmology, The First Affiliated Hospital of Xi'an Medical University, No.48 West Fenghao Road, Xi'an, 710077, Shaanxi, China. Electronic address: rechelrong198222@163.com. ; Guomin Yao:1.Department of Ophthalmology, The First Affiliated Hospital of Xi'an Medical University, No.48 West Fenghao Road, Xi'an, 710077, Shaanxi, China. ; Lingxiao Zhou:1.Department of Ophthalmology, The First Affiliated Hospital of Xi'an Medical University, No.48 West Fenghao Road, Xi'an, 710077, Shaanxi, China. ; Min Zhang:1.Department of Endocrinology, The First Affiliated Hospital of Xi'an Medical University, No.48 West Fenghao Road, Xi'an, 710077, Shaanxi, China. ; Jin Yan:1.College of Medical Technology of Xi'an Medical University, No.1 Xinwang Road, Xi'an, 710021, Shaanxi, China. ; Xiaodi Wang:1.Department of Ophthalmology, The First Affiliated Hospital of Xi'an Medical University, No.48 West Fenghao Road, Xi'an, 710077, Shaanxi, China. ; Ya Li:1.Department of Endocrinol
期刊:Autophagy is required for the promoting effect of angiogenic factor with G patch domain and forkhead-associated domain 1 (AGGF1) in retinal angiogenesis
影响因子 :3.1
引用产品: RF/6A 细胞
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Angiogenic factor with G patch and FHA domains 1 protects retinal vascular endothelial cells under hyperoxia by inhibiting autophagy (2007-00-20)
作者:Guomin Yao:1.Department of Ophthalmology, The First Affiliated Hospital, Xi'an Medical University, Xi'an, China. ; Rong Li:1.Department of Ophthalmology, The First Affiliated Hospital, Xi'an Medical University, Xi'an, China. ; Junhui Du:1.Department of Ophthalmology, Xi'an Ninth Hospital Affiliated to Medical College of Xi'an Jiaotong University, Xi'an, China. ; Yang Yao:1.Department of Central laboratory, The First Affiliated Hospital, Xi'an Medical University, Xi'an, China. ;
期刊:Angiogenic factor with G patch and FHA domains 1 protects retinal vascular endothelial cells under hyperoxia by inhibiting autophagy
影响因子 :3.6
引用产品: RF/6A 细胞
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Adiponectin inhibits high glucose‐induced angiogenesis via inhibiting autophagy in RF/6A cells (2004-09-20)
作者:Rong Li:1.Department of Ophthalmology, The First Affiliated Hospital of Xi'an Medical University, Xi'an, Shaanxi, People's Republic of China. ; Junhui Du:1.Department of Ophthalmology, Xi'an Ninth Hospital Affiliated to Medical College of Xi'an Jiaotong University, Xi'an, Shaanxi, People's Republic of China. ; Yang Yao:1.Department of Central Laboratory, The First Affiliated Hospital of Xi'an Medical University, Xi'an, Shaanxi, People's Republic of China. ; Guomin Yao:1.Department of Ophthalmology, The First Affiliated Hospital of Xi'an Medical University, Xi'an, Shaanxi, People's Republic of China. ; Xiaodi Wang:1.Department of Ophthalmology, The First Affiliated Hospital of Xi'an Medical University, Xi'an, Shaanxi, People's Republic of China. ;
期刊:Adiponectin inhibits high glucose‐induced angiogenesis via inhibiting autophagy in RF/6A cells
影响因子 :5.6
引用产品: RF/6A 细胞
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