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产品概述
MEM培养基（Minimum Essential Medium）也称最低必需培养基、最小基本培养基或低限量Eagle培养基，由Harry Eagle在Eagle基本培养基（BEM）的基础上发展而来的，是一种最基本、适用范围最广的培养基，是动物细胞培养中最常用的培养基之一。MEM培养基仅含有12种必需氨基酸、谷氨酰胺和8种维生素，成分简单，主要用于贴壁细胞的培养，配方修改后也可用于其他类型细胞培养。ATCC改良的MEM培养基含非必须氨基酸、丙酮酸钠，并降低了碳酸氢钠的用量。
本产品含有多类细胞培养所需的氨基酸、维生素、无机盐等多种成分，但不含蛋白质、脂类或任何生长因子，故此产品需搭配血清或无血清添加物使用。
成分说明
形态 | 液体 |
浓度 | 1× |
规格 | 500mL |
pH | 7.2-7.4 |
平衡盐 | Earle’s 盐 |
NEAA | 含 |
L-谷氨酰胺 | 2 mM |
丙酮酸钠 | 1 mM |
NaHCO3 | 1500 mg/L |
D-葡萄糖 | 1000 mg/L |
HEPES缓冲剂 | 无 |
酚红指示剂 | 10 mg/L |
储存条件 | 2-8℃，避光 |
运输条件 | 常温 |
有效期 | 18个月 |
注意事项
1、本产品经0.1 μm过滤除菌，使用时应注意无菌操作，避免污染；
2、为保持本产品的最佳使用效果，请勿进行冻融处理；
3、本产品仅用于科研或进一步研究使用，不用于诊断和治疗。
参考文献
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Discovery of Novel Coumarin-quinolinium Derivatives as Pan-KRAS Translation Inhibitors by Targeting 5′-UTR RNA G-Quadruplexes (2024-01-25)
作者：Mao-Lin Li:1.School of Pharmaceutical Sciences, Guangdong Provincial Key Laboratory of New Drug Design and Evaluation, Sun Yat-sen University, Guangzhou, Guangdong 510006, China. ; Le-Tian Dai:1.School of Pharmaceutical Sciences, Guangdong Provincial Key Laboratory of New Drug Design and Evaluation, Sun Yat-sen University, Guangzhou, Guangdong 510006, China. ; Zhuo-Yu Gao:1.School of Pharmaceutical Sciences, Guangdong Provincial Key Laboratory of New Drug Design and Evaluation, Sun Yat-sen University, Guangzhou, Guangdong 510006, China. ; Jia-Tong Yan:1.School of Pharmaceutical Sciences, Guangdong Provincial Key Laboratory of New Drug Design and Evaluation, Sun Yat-sen University, Guangzhou, Guangdong 510006, China. ; Shu-Min Xu:1.School of Pharmaceutical Sciences, Guangdong Provincial Key Laboratory of New Drug Design and Evaluation, Sun Yat-sen University, Guangzhou, Guangdong 510006, China. ; Jia-Heng Tan:1.School of Pharmaceutical Sciences, Guangdong Provincial Key Laboratory
期刊：Discovery of Novel Coumarin-quinolinium Derivatives as Pan-KRAS Translation Inhibitors by Targeting 5′-UTR RNA G-Quadruplexes
DOI：10.1021/acs.jmedchem.3c01773
影响因子 ：7.3
引用产品： MIA PaCa-2 细胞 , DMEM高糖 培养基 , 293 [HEK-293] 细胞 , HPAF-II 细胞 , U-87 MG 细胞 , MEM (ATCC改良) 培养基 , PANC-1 细胞 , HCT 116 细胞 , HFL1 细胞
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SPI1 Mediates N-Myristoyltransferase 1 to Advance Gastric Cancer Progression via PI3K/AKT/mTOR Pathway (2023-03-17)
作者：Ping Qiu:1.Department of Gastroenterology, Jiangxi Pingxiang People's Hospital, Pingxiang, Jiangxi, China. ; Xing Li:1.Department of Gastroenterology, Jiangxi Pingxiang People's Hospital, Pingxiang, Jiangxi, China. ; Min Gong:1.Department of Gastroenterology, Jiangxi Pingxiang People's Hospital, Pingxiang, Jiangxi, China. ; Ping Wen:1.Department of Gastroenterology, Jiangxi Pingxiang People's Hospital, Pingxiang, Jiangxi, China. ; Jianbo Wen:1.Department of Gastroenterology, Jiangxi Pingxiang People's Hospital, Pingxiang, Jiangxi, China. ; Linfang Xu:1.Department of Gastroenterology, Jiangxi Pingxiang People's Hospital, Pingxiang, Jiangxi, China. ; Guiliang Wang:1.Department of Gastroenterology, Jiangxi Pingxiang People's Hospital, Pingxiang, Jiangxi, China. ;
期刊：SPI1 Mediates N-Myristoyltransferase 1 to Advance Gastric Cancer Progression via PI3K/AKT/mTOR Pathway
影响因子 ：2.7
引用产品： GES-1 细胞 , AGS 细胞 , HGC-27 细胞 , SNU-5 细胞 , MKN-45 细胞 , RPMI-1640 培养基 , 特级胎牛血清 , 青霉素-链霉素溶液 (双抗)，100× , Ham's F-12 培养基 , IMDM 培养基 , 293 [HEK-293] 细胞 , MEM (ATCC改良) 培养基
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Proinflammatory Bone Marrow Mesenchymal Stem Cell-Derived Exosomal miR-150-3p Suppresses Proinflammatory Polarization of Alveolar Macrophages in Sepsis by Targeting Inhibin Subunit Beta A (2023-10-11)
作者：Guojin Liang:1.Department of Anesthesiology, Ningbo First Hospital, Ningbo, China. ; Yueying Feng:1.Department of Pediatrics, Ningbo Women & Children's Hospital, Ningbo, China. ; Wan Tang:1.Department of Anesthesiology, Ningbo First Hospital, Ningbo, China. ; Lifeng Yao:1.Department of Anesthesiology, Ningbo First Hospital, Ningbo, China. ; Changshun Huang:1.Department of Anesthesiology, Ningbo First Hospital, Ningbo, China. ; Yijun Chen:1.Department of Anesthesiology, Ningbo First Hospital, Ningbo, China. ;
期刊：Proinflammatory Bone Marrow Mesenchymal Stem Cell-Derived Exosomal miR-150-3p Suppresses Proinflammatory Polarization of Alveolar Macrophages in Sepsis by Targeting Inhibin Subunit Beta A
影响因子 ：2.3
引用产品： 293 [HEK-293] 细胞 , MEM (ATCC改良) 培养基
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KLF5-mediated aquaporin 3 activated autophagy to facilitate cisplatin resistance of gastric cancer (2022-09-20)
作者：Xudong Dai:1.Department of General Surgery, Lianshui People's Hospital Affiliated to Kangda College of Nanjing Medical University, Nanjing, China. ; Yong Chen:1.Division of Gastrointestinal Surgery, Department of General Surgery, The Affiliated Huai'an Hospital of Xuzhou Medical University, Huai'an, China. ; Ning Chen:1.Division of Gastrointestinal Surgery, Department of General Surgery, The Affiliated Huai'an Hospital of Xuzhou Medical University, Huai'an, China. ; Jin Dou:1.Division of Gastrointestinal Surgery, Department of General Surgery, The Affiliated Huai'an Hospital of Xuzhou Medical University, Huai'an, China. ; Haiwen Zhuang:1.Division of Gastrointestinal Surgery, Department of General Surgery, The Affiliated Huai'an Hospital of Xuzhou Medical University, Huai'an, China. ; Jian Wang:1.Division of Gastrointestinal Surgery, Department of General Surgery, The Affiliated Huai'an Hospital of Xuzhou Medical University, Huai'an, China. ; Xin Zhao:1.Division of Gastrointestina
期刊：KLF5-mediated aquaporin 3 activated autophagy to facilitate cisplatin resistance of gastric cancer
DOI：10.1080/08923973.2022.2122498
影响因子 ：3.7
引用产品： 293 [HEK-293] 细胞 , 青霉素-链霉素溶液 (双抗)，100× , MEM (ATCC改良) 培养基
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Cancer stem cell-like cells-derived exosomal lncRNA CDKN2B-AS1 promotes biological characteristics in thyroid cancer via miR-122-5p/P4HA1 axis (2022-12-14)
作者：Qinghua Wu:1.Department of General Surgery, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, China. ; Yonggang He:1.Department of General Surgery, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, China. ; Xin Liu:1.Department of General Surgery, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, China. ; Fangxiu Luo:1.Department of General Surgery, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, China. ; Yimei Jiang:1.Department of General Surgery, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, China. ; Ming Xiang:1.Department of General Surgery, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, China. ; Ren Zhao:1.Department of General Surgery, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, China. ;
期刊：Cancer stem cell-like cells-derived exosomal lncRNA CDKN2B-AS1 promotes biological characteristics in thyroid cancer via miR-122-5p/P4HA1 axis
DOI：10.1016/j.reth.2022.11.005
影响因子 ：3.7
引用产品： RPMI-1640 培养基 , 特级胎牛血清 , 293 [HEK-293] 细胞 , MEM (ATCC改良) 培养基
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The anticancer effect of EGFR-targeting artificial microRNA controlled by SLPI promoter in nasopharyngeal carcinoma (2022-10-25)
作者：Feilin Dong:1.Medical College, Soochow University, Suzhou, China. ; 2.Department of Otolaryngology, Otolaryngology & Head and Neck Center, Cancer Center, Zhejiang Provincial People's Hospital (Affiliated People's Hospital, Hangzhou Medical College), Hangzhou, China. ; Lizhong Su:1.Department of Otolaryngology, Otolaryngology & Head and Neck Center, Cancer Center, Zhejiang Provincial People's Hospital (Affiliated People's Hospital, Hangzhou Medical College), Hangzhou, China. ; Jun Tan:1.Department of Otolaryngology, Otolaryngology & Head and Neck Center, Cancer Center, Zhejiang Provincial People's Hospital (Affiliated People's Hospital, Hangzhou Medical College), Hangzhou, China. ; Hui Luo:1.Department of Otolaryngology, Otolaryngology & Head and Neck Center, Cancer Center, Zhejiang Provincial People's Hospital (Affiliated People's Hospital, Hangzhou Medical College), Hangzhou, China. ;
期刊：The anticancer effect of EGFR-targeting artificial microRNA controlled by SLPI promoter in nasopharyngeal carcinoma
影响因子 ：3.1
引用产品： 293 [HEK-293] 细胞 , 特级胎牛血清 , 青霉素-链霉素溶液 (双抗)，100× , RPMI-1640 培养基 , DMEM高糖 培养基 , MEM (ATCC改良) 培养基
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