产品中心Cell Resources
联系我们CONTACT US
400-999-210024小时服务热线
产品概述
RPMI-1640是Moore等人于1967年在美国纽约州法罗市的罗斯韦尔公园纪念研究所（Roswell Park Memorial Institute, RPMI）开发出来的，RPMI是该研究所开发的一类细胞培养基，1640是培养基代号。RPMI-1640是改进型的McCoy's 5A培养基，使用碳酸氢盐缓冲系统，与大多数哺乳动物细胞培养基不同的是其典型的PH8的配方。RPMI-1640培养基最初是为淋巴细胞培养专门设计的，现在已广泛应用于各种正常细胞和癌细胞的培养，尤其是悬浮细胞的培养，是使用最为广泛的培养基之一。
酚红在培养基中被用来作为pH值的指示剂，用以持续监测培养液的酸碱度，在低pH值时酚红使培养液呈黄色，而较高的pH值时使培养液呈紫色，pH值7.2-7.4时为红色，最适合细胞培养。但酚红也有一些缺点，研究表明酚红可以模拟固醇类激素（特别是雌激素）的作用，因此在用到雌激素敏感的细胞时（如乳腺组织），最好使用不含酚红的培养基。酚红会干扰流式细胞分析时候的检测。此外，一些无血清培养基的配方中若存在酚红会干扰钠-钾平衡。
本产品含有多类细胞培养所需的氨基酸、维生素、无机盐等多种成分；但不含蛋白质、脂类或任何生长因子；故此产品需搭配血清或无血清添加物使用。
成分说明
形态 | 液体 |
浓度 | 1× |
规格 | 500mL |
pH | 7.2-7.4 |
L-谷氨酰胺 | 2 mM |
NaHCO3 | 2000 mg/L |
D-葡萄糖 | 2000 mg/L |
HEPES缓冲剂 | 无 |
酚红指示剂 | 无 |
储存条件 | 2-8℃，避光 |
运输条件 | 常温 |
有效期 | 18个月 |
注意事项
1、本产品经0.1μm过滤除菌，使用时应注意无菌操作，避免污染；
2、为保持本产品的最佳使用效果，请勿进行冻融处理；
3、本产品仅用于科研或进一步研究使用，不用于诊断和治疗。
参考文献
-
A fluorescence-based assay for Trichomonas vaginalis drug screening (2023-09-18)
作者：Qianqian Chen:1.NHC Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100005, People's Republic of China. ; Jingzhong Li:1.NHC Key Laboratory of Echinococcosis, Prevention and Control, Tibet Autonomous Region Center for Disease Control and Prevention, 21# Linkuo North Road, Chengguan District, Lhasa, 850000, People's Republic of China. ; Zhensheng Wang:1.Department of Microbiology and Parasitology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and School of Basic Medicine, Peking Union Medical College, Beijing, 100005, People's Republic of China. ; Wei Meng:1.NHC Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100005, People's Republic of China. ; Heng Wang:1.Department of Microbiology and Parasitology, Institute of Basic Medical Sciences, C
期刊：A fluorescence-based assay for Trichomonas vaginalis drug screening
DOI：10.1186/s13071-023-05919-6
影响因子 ：3.2
引用产品： RPMI-1640 (不含酚红) 培养基
-
Neonicotinoid insecticides promote breast cancer progression via G protein-coupled estrogen receptor: In vivo, in vitro and in silico studies (2022-10-06)
作者：Xin Li:1.College of Resources and Environment, Hunan Agricultural University, 1, Nongda Road, Furong District, Changsha 410128, China. ; Sen He:1.College of Resources and Environment, Hunan Agricultural University, 1, Nongda Road, Furong District, Changsha 410128, China. ; Han Xiao:1.State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou 350002, China. ; Ting-Ting He:1.College of Resources and Environment, Hunan Agricultural University, 1, Nongda Road, Furong District, Changsha 410128, China. ; Jia-Da Zhang:1.College of Resources and Environment, Hunan Agricultural University, 1, Nongda Road, Furong District, Changsha 410128, China. ; Zi-Rui Luo:1.College of Resources and Environment, Hunan Agricultural University, 1, Nongda Road, Furong District, Changsha 410128, China. ; Jie-Zhi Ma:1.Department of Obstetrics and Gynecology, Xiangya Third Hospital, Central South University, Changsha 410013, China
期刊：Neonicotinoid insecticides promote breast cancer progression via G protein-coupled estrogen receptor: In vivo, in vitro and in silico studies
DOI：10.1016/j.envint.2022.107568
影响因子 ：13.4
引用产品： 4T1 细胞 , RPMI-1640 培养基 , 青霉素-链霉素溶液 (双抗)，100× , RPMI-1640 (不含酚红) 培养基
-
Hugan Qingzhi medication ameliorates free fatty acid-induced L02 hepatocyte endoplasmic reticulum stress by regulating the activation of PKC-δ (2012-00-20)
作者：Miaoting Yang:1.Department of Pharmacy, People's Hospital of Longhua, Shenzhen, 518109, Guangdong, China. ; Zhijuan Chen:1.Department of Pharmacy, The Seventh Affiliated Hospital of Sun Yat-sen University, Shenzhen, 518107, Guangdong, China. ; Shijian Xiang:1.Department of Pharmacy, The Seventh Affiliated Hospital of Sun Yat-sen University, Shenzhen, 518107, Guangdong, China. ; Fan Xia:1.Department of Pharmacy, The Seventh Affiliated Hospital of Sun Yat-sen University, Shenzhen, 518107, Guangdong, China. ; Waijiao Tang:1.Department of Pharmacy, Zhujiang Hospital, Southern Medical University, Guangzhou, 510282, Guangdong, China. ; Xiaorui Yao:1.Department of Pharmacy, Shantou Central Hospital, Affiliated Shantou Hospital of Sun Yat-sen University, Shantou, 515041, Guangdong, China. ; Benjie Zhou:1.Department of Pharmacy, The Seventh Affiliated Hospital of Sun Yat-sen University, Shenzhen, 518107, Guangdong, China. zhoubj163@163.com. ;
期刊：Hugan Qingzhi medication ameliorates free fatty acid-induced L02 hepatocyte endoplasmic reticulum stress by regulating the activation of PKC-δ
DOI：10.1186/s12906-020-03164-3
影响因子 ：3.9
引用产品： RPMI-1640 (不含酚红) 培养基
FAQs
Q：{{item.question}}
A：
产品资料
识别码示意图
