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产品概述
RPMI-1640是Moore等人于1967年在美国纽约州法罗市的罗斯韦尔公园纪念研究所（Roswell Park Memorial Institute, RPMI）开发出来的，RPMI是该研究所开发的一类细胞培养基，1640是培养基代号。RPMI-1640是改进型的McCoy's 5A培养基，使用碳酸氢盐缓冲系统，与大多数哺乳动物细胞培养基不同的是其典型的PH8的配方。RPMI-1640培养基最初是为淋巴细胞培养专门设计的，现在已广泛应用于各种正常细胞和癌细胞的培养，尤其是悬浮细胞的培养，是使用最为广泛的培养基之一。ATCC改良的RPMI-1640额外添加了丙酮酸钠和HEPES，增加了糖的含量，降低了碳酸氢钠的含量。
本产品含有多类细胞培养所需的氨基酸、维生素、无机盐等多种成分，但不含蛋白质、脂类或任何生长因子，故此产品需搭配血清或无血清添加物使用。
成分说明
形态 | 液体 |
浓度 | 1× |
规格 | 500mL |
pH | 7.2-7.4 |
L-谷氨酰胺 | 2 mM |
NaHCO3 | 1500 mg/L |
D-葡萄糖 | 4500 mg/L |
丙酮酸钠 | 1 mM |
HEPES缓冲剂 | 10 mM |
酚红指示剂 | 5 mg/L |
储存条件 | 2-8℃，避光 |
运输条件 | 常温 |
有效期 | 18个月 |
注意事项
1、本产品经0.1 μm过滤除菌，使用时应注意无菌操作，避免污染；
2、为保持本产品的最佳使用效果，请勿进行冻融处理；
3、本产品仅用于科研或进一步研究使用，不用于诊断和治疗。
参考文献
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Atherosis-associated lnc_000048 activates PKR to enhance STAT1-mediated polarization of THP-1 macrophages to M1 phenotype (2024-03-08)
作者：Yuanyuan Ding:1.Department of Neurology, The Affiliated Hospital of Qingdao University, Qingdao, Shandong Province, China. ; Yu Sun:1.Department of Neurology, The Affiliated Hospital of Qingdao University, Qingdao, Shandong Province, China. ; Hongyan Wang:1.Qingdao Cadre Health Care Service Center, The Affiliated Hospital of Qingdao University, Qingdao, Shandong Province, China. ; Hongqin Zhao:1.Department of Neurology, The Affiliated Hospital of Qingdao University, Qingdao, Shandong Province, China. ; Ruihua Yin:1.Department of Neurology, The Affiliated Hospital of Qingdao University, Qingdao, Shandong Province, China. ; Meng Zhang:1.Department of Neurology, The Affiliated Hospital of Qingdao University, Qingdao, Shandong Province, China. ; Xudong Pan:1.Department of Neurology, The Affiliated Hospital of Qingdao University, Qingdao, Shandong Province, China. ; Xiaoyan Zhu:1.Department of Critical Care Medicine, The Affiliated Hospital of Qingdao University, Qingdao, Shandong Pr
期刊：Atherosis-associated lnc_000048 activates PKR to enhance STAT1-mediated polarization of THP-1 macrophages to M1 phenotype
DOI：10.4103/NRR.NRR-D-23-01355
影响因子 ：6.1
引用产品： THP-1 细胞 , RPMI-1640 (ATCC改良) 培养基 , 青霉素-链霉素溶液 (双抗)，100× , β-巯基乙醇 (55mM)，1000×
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Stachydrine represses the proliferation and enhances cell cycle arrest and apoptosis of breast cancer cells via PLA2G2A/DCN axis (2024-01-01)
作者：Zhen Zhai:1.Mammary Department, Dongfang Hospital Beijing University of Chinese Medicine, Beijing, China. ; Tianlong Mu:1.Pathology Department, Dongfang Hospital Beijing University of Chinese Medicine, Beijing, China. ; Lina Zhao:1.Mammary Department, Dongfang Hospital Beijing University of Chinese Medicine, Beijing, China. ; Dongsheng Zhu:1.Mammary Department, Dongfang Hospital Beijing University of Chinese Medicine, Beijing, China. ; Xin Zhong:1.Mammary Department, Dongfang Hospital Beijing University of Chinese Medicine, Beijing, China. ; Yiliang Li:1.Mammary Department, Dongfang Hospital Beijing University of Chinese Medicine, Beijing, China. ; Chen Liang:1.Mammary Department, Dongfang Hospital Beijing University of Chinese Medicine, Beijing, China. ; Wei Li:1.Mammary Department, Dongfang Hospital Beijing University of Chinese Medicine, Beijing, China. ; Qingyuan Zhou:1.Mammary Department, Dongfang Hospital Beijing University of Chinese Medicine, Beijing, China. ;
期刊：Stachydrine represses the proliferation and enhances cell cycle arrest and apoptosis of breast cancer cells via PLA2G2A/DCN axis
影响因子 ：3.0
引用产品： MCF 10A 细胞 , MCF7 [MCF-7] 细胞 , MDA-MB-231 细胞 , RPMI-1640 (ATCC改良) 培养基 , Leibovitz's L-15 培养基
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Inhibition of glycolysis represses the growth and alleviates the endoplasmic reticulum stress of breast cancer cells by regulating TMTC3 (2023-04-10)
作者：Xue Hu:1.Department of Breast Surgery, China-Japan Union Hospital of Jilin University, Changchun City, Jilin Province, 130033, China. ; Baoliang Guo:1.Department of General Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin City, Heilongjiang Province, China. ; Tong Sun:1.Department of Breast Surgery, China-Japan Union Hospital of Jilin University, Changchun City, Jilin Province, 130033, China. ; Wan Wang:1.Department of Breast Surgery, China-Japan Union Hospital of Jilin University, No. 126 Xiantai Avenue, Nanguan District, Changchun City, Jilin Province, 130033, China. ;
期刊：Inhibition of glycolysis represses the growth and alleviates the endoplasmic reticulum stress of breast cancer cells by regulating TMTC3
影响因子 ：2.1
引用产品： MCF 10A 细胞 , MCF7 [MCF-7] 细胞 , MDA-MB-231 细胞 , MDA-MB-436 细胞 , SK-BR-3 [SKBR3] 细胞 , DMEM/F12 培养基 , 马血清 , MEM非必需氨基酸溶液 (NEAA)，100× , 青霉素-链霉素溶液 (双抗)，100× , RPMI-1640 (ATCC改良) 培养基 , Leibovitz's L-15 培养基 , McCoy's 5A 培养基 , 特级胎牛血清
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