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产品概述
DMEM/F12培养基是在DMEM培养基的基础上，添加F12培养基中更为丰富的营养成分，含有多种微量元素，广泛应用于多种哺乳类细胞的培养。同时，DMEM/F12培养基常作为开发无血清培养基的基础，也适用于低血清含量下哺乳动物细胞的培养以及克隆密度培养。不含葡萄糖的DMEM/F12培养基可以根据研究需要，随意调节葡萄糖的浓度，方便快捷。
HEPES是一种优良的生物缓冲剂，对细胞无毒性作用，添加HEPES的培养基能够较长时间保持恒定的pH范围，可以有效的防止培养液pH波动较大对细胞生长产生不利的影响。
本产品含有多类细胞培养所需的氨基酸、维生素、无机盐等多种成分，但不含蛋白质、脂类或任何生长因子，故此产品需搭配血清或无血清添加物使用。
成分说明
形态 | 液体 |
浓度 | 1× |
规格 | 500mL |
pH | 7.2-7.4 |
L-谷氨酰胺 | 2.5 mM |
NaHCO3 | 1200 mg/L |
D-葡萄糖 | 无 |
丙酮酸钠 | 0.5 mM |
HEPES缓冲剂 | 15 mM |
酚红指示剂 | 8.1 mg/L |
储存条件 | 2-8℃，避光 |
运输条件 | 常温 |
有效期 | 18个月 |
注意事项
1、本产品经0.1 μm过滤除菌，使用时应注意无菌操作，避免污染；
2、为保持本产品的最佳使用效果，请勿进行冻融处理；
3、本产品仅用于科研或进一步研究使用，不用于诊断和治疗。
参考文献
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Psychological stress-induced microbial metabolite indole-3-acetate disrupts intestinal cell lineage commitment (2024-01-23)
作者：Wei Wei:1.State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 211198, China; Laboratory of Metabolic Regulation and Drug Target Discovery, School of Pharmacy, China Pharmaceutical University, Nanjing 210009, China. ; Yali Liu:1.State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 211198, China; Laboratory of Metabolic Regulation and Drug Target Discovery, School of Pharmacy, China Pharmaceutical University, Nanjing 210009, China; State Key Laboratory of Digestive Diseases, The Chinese University of Hong Kong, Hong Kong 999077, China. ; Yuanlong Hou:1.State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 211198, China; Laboratory of Metabolic Regulation and Drug Target Discovery, School of Pharmacy, China Pharmaceutical University, Nanjing 210009, China; Department of Pharmacy, Shenzhen Luohu People's Hospital, Shenzhen 518005, China. ; Shuqi Cao:1.State Key Laboratory of Natural Medicines, C
期刊：Psychological stress-induced microbial metabolite indole-3-acetate disrupts intestinal cell lineage commitment
DOI：10.1016/j.cmet.2023.12.026
影响因子 ：29.0
引用产品： DMEM/F12无糖 (含HEPES) 培养基
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Melatonin Alleviates the Oxygen–Glucose Deprivation/Reperfusion-Induced Pyroptosis of HEI-OC1 Cells and Cochlear Hair Cells via MT-1,2/Nrf2 (NFE2L2)/ROS/NLRP3 Pathway (2022-11-05)
作者：Yu Zheng:1.Department of Otorhinolaryngology, Eye Ear Nose and Throat Hospital, Fudan University, Shanghai, 200031, China. ; 2.Shanghai Clinical Medical Center of Hearing Medicine, Shanghai, 200031, China. ; 3.NHC Key Laboratory of Hearing Medicine, Fudan University, Shanghai, 20031, People's Republic of China. ; 4.Research Institute of Otorhinolaryngology, Fudan University, Shanghai, 200031, China. ; Na Gao:1.Department of Otorhinolaryngology, Eye Ear Nose and Throat Hospital, Fudan University, Shanghai, 200031, China. ; 2.Shanghai Clinical Medical Center of Hearing Medicine, Shanghai, 200031, China. ; 3.NHC Key Laboratory of Hearing Medicine, Fudan University, Shanghai, 20031, People's Republic of China. ; 4.Research Institute of Otorhinolaryngology, Fudan University, Shanghai, 200031, China. ; Weixun Zhang:1.Department of Otorhinolaryngology, Eye Ear Nose and Throat Hospital, Fudan University, Shanghai, 200031, China. ; 2.Shanghai Clinical Medical Center of Hearing Medicine, Shang
期刊：Melatonin Alleviates the Oxygen–Glucose Deprivation/Reperfusion-Induced Pyroptosis of HEI-OC1 Cells and Cochlear Hair Cells via MT-1,2/Nrf2 (NFE2L2)/ROS/NLRP3 Pathway
DOI：10.1007/s12035-022-03077-x
影响因子 ：5.7
引用产品： DMEM/F12 (含HEPES) 培养基 , DMEM高糖 培养基 , DMEM无糖 (含L-丙氨酰-L-谷氨酰胺、HEPES) 培养基 , DMEM/F12无糖 (含HEPES) 培养基
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