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产品概述
DMEM/F12培养基是在DMEM培养基的基础上，添加F12培养基中更为丰富的营养成分，含有多种微量元素，广泛应用于多种哺乳类细胞的培养。同时，DMEM/F12培养基常作为开发无血清培养基的基础，也适用于低血清含量下哺乳动物细胞的培养以及克隆密度培养。
HEPES是一种优良的生物缓冲剂，对细胞无毒性作用，添加HEPES的培养基能够较长时间保持恒定的pH范围，可以有效的防止培养液pH波动较大对细胞生长产生不利的影响。
本产品含有多类细胞培养所需的氨基酸、维生素、无机盐等多种成分，但不含蛋白质、脂类或任何生长因子，故此产品需搭配血清或无血清添加物使用。
成分说明
形态 | 液体 |
浓度 | 1× |
规格 | 500mL |
pH | 7.2-7.4 |
L-谷氨酰胺 | 2.5 mM |
NaHCO3 | 1200 mg/L |
D-葡萄糖 | 3151 mg/L |
丙酮酸钠 | 0.5 mM |
HEPES缓冲剂 | 15 mM |
酚红指示剂 | 8.1 mg/L |
储存条件 | 2-8℃，避光 |
运输条件 | 常温 |
有效期 | 18个月 |
注意事项
1、本产品经0.1 μm过滤除菌，使用时应注意无菌操作，避免污染；
2、为保持本产品的最佳使用效果，请勿进行冻融处理；
3、本产品仅用于科研或进一步研究使用，不用于诊断和治疗。
参考文献
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Circumventing Drug Resistance in Gastric Cancer: A Spatial Multi-omics Exploration of Chemo and Immuno-Therapeutic Response Dynamics (2024-03-19)
作者：Gang Che:1.Department of Surgical Oncology, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310016, China. ; Jie Yin:1.Department of Colorectal Medicine, Zhejiang Cancer Hospital, Hangzhou Institute of Medicine (HIM), Chinese Academy of Sciences, Hangzhou, Zhejiang 310022, China. ; Wankun Wang:1.Department of Surgical Oncology, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310016, China. ; Yandong Luo:1.Zhejiang Provincial Key Laboratory of Pancreatic Disease, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310016, China. ; Yiran Chen:1.Department of Surgical Oncology, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310016, China. ; Xiongfei Yu:1.Department of Surgical Oncology, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310016, China. ; Haiyong Wang:1.Department of Surgic
期刊：Circumventing Drug Resistance in Gastric Cancer: A Spatial Multi-omics Exploration of Chemo and Immuno-Therapeutic Response Dynamics
DOI：10.1016/j.drup.2024.101080
影响因子 ：24.3
引用产品： DMEM/F12 (含HEPES) 培养基
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Bioinformatics-Based Screening of Key LncRNAs for Modulating the Transcriptome Associated with Glaucoma in Human Trabecular Meshwork Cells (2024-02-29)
作者：Junhong Guo:1.Shenzhen Eye Hospital, Jinan University, Shenzhen Eye Institute, 518040 Shenzhen, Guangdong, China. ; Yunfei Wu:1.Center for Genome Analysis, Ruixing Biotechnology Co., Ltd., 430075 Wuhan, Hubei, China. ; Yue Sun:1.Center for Genome Analysis, Ruixing Biotechnology Co., Ltd., 430075 Wuhan, Hubei, China. ; Dong Chen:1.Center for Genome Analysis, Ruixing Biotechnology Co., Ltd., 430075 Wuhan, Hubei, China. ; Yijia Huang:1.Shenzhen Eye Hospital, Jinan University, Shenzhen Eye Institute, 518040 Shenzhen, Guangdong, China. ; Xiaoli Shen:1.Shenzhen Eye Hospital, Jinan University, Shenzhen Eye Institute, 518040 Shenzhen, Guangdong, China. ; Zhichao Yan:1.Shenzhen Eye Hospital, Jinan University, Shenzhen Eye Institute, 518040 Shenzhen, Guangdong, China. ; Jiantao Wang:1.Shenzhen Eye Hospital, Jinan University, Shenzhen Eye Institute, 518040 Shenzhen, Guangdong, China. ;
期刊：Bioinformatics-Based Screening of Key LncRNAs for Modulating the Transcriptome Associated with Glaucoma in Human Trabecular Meshwork Cells
影响因子 ：3.1
引用产品： DMEM/F12 (含HEPES) 培养基
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Glioma angiogenesis is boosted by ELK3 activating the HIF-1 $$\alpha$$ /VEGF-A signaling axis (2023-07-14)
作者：Mou Yueyang:1.College of Life Sciences, Northwest University, Xi'an, China. ; 2.Departments of Neurosurgery, Tangdu Hospital, Air Force Medical University, Xi'an, China. ; Hu Yaqin:1.College of Life Sciences, Northwest University, Xi'an, China. ; Xue Guolian:1.College of Life Sciences, Northwest University, Xi'an, China. ; Zhao Wenjian:1.Departments of Neurosurgery, Tangdu Hospital, Air Force Medical University, Xi'an, China. ; Jiao Yang:1.Departments of Neurosurgery, Tangdu Hospital, Air Force Medical University, Xi'an, China. ; Li Chen:1.Departments of Neurosurgery, Tangdu Hospital, Air Force Medical University, Xi'an, China. ; Cao Haiyan:1.Departments of Neurosurgery, Tangdu Hospital, Air Force Medical University, Xi'an, China. ; Chao Min:1.Departments of Neurosurgery, Tangdu Hospital, Air Force Medical University, Xi'an, China. ; Deng Jianping:1.Departments of Neurosurgery, Tangdu Hospital, Air Force Medical University, Xi'an, China. ; Dai Penggao:1.College of Life Science
期刊：Glioma angiogenesis is boosted by ELK3 activating the HIF-1 $$\alpha$$ /VEGF-A signaling axis
DOI：10.1186/s12885-023-11069-w
影响因子 ：3.8
引用产品： DMEM/F12 (含HEPES) 培养基
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Melatonin Alleviates the Oxygen–Glucose Deprivation/Reperfusion-Induced Pyroptosis of HEI-OC1 Cells and Cochlear Hair Cells via MT-1,2/Nrf2 (NFE2L2)/ROS/NLRP3 Pathway (2022-11-05)
作者：Yu Zheng:1.Department of Otorhinolaryngology, Eye Ear Nose and Throat Hospital, Fudan University, Shanghai, 200031, China. ; 2.Shanghai Clinical Medical Center of Hearing Medicine, Shanghai, 200031, China. ; 3.NHC Key Laboratory of Hearing Medicine, Fudan University, Shanghai, 20031, People's Republic of China. ; 4.Research Institute of Otorhinolaryngology, Fudan University, Shanghai, 200031, China. ; Na Gao:1.Department of Otorhinolaryngology, Eye Ear Nose and Throat Hospital, Fudan University, Shanghai, 200031, China. ; 2.Shanghai Clinical Medical Center of Hearing Medicine, Shanghai, 200031, China. ; 3.NHC Key Laboratory of Hearing Medicine, Fudan University, Shanghai, 20031, People's Republic of China. ; 4.Research Institute of Otorhinolaryngology, Fudan University, Shanghai, 200031, China. ; Weixun Zhang:1.Department of Otorhinolaryngology, Eye Ear Nose and Throat Hospital, Fudan University, Shanghai, 200031, China. ; 2.Shanghai Clinical Medical Center of Hearing Medicine, Shang
期刊：Melatonin Alleviates the Oxygen–Glucose Deprivation/Reperfusion-Induced Pyroptosis of HEI-OC1 Cells and Cochlear Hair Cells via MT-1,2/Nrf2 (NFE2L2)/ROS/NLRP3 Pathway
DOI：10.1007/s12035-022-03077-x
影响因子 ：5.7
引用产品： DMEM/F12 (含HEPES) 培养基 , DMEM高糖 培养基 , DMEM无糖 (含L-丙氨酰-L-谷氨酰胺、HEPES) 培养基 , DMEM/F12无糖 (含HEPES) 培养基
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Copper Tannic Acid-Coordinated Metal–Organic Nanosheets for Synergistic Antimicrobial and Antifouling Coatings (2002-01-20)
作者：Jia Li:1.Key Laboratory of Marine Materials and Related Technologies, Zhejiang Key Laboratory of Marine Materials and Protective Technologies, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo 315201, China. ; 2.Nano Science and Technology Institute, University of Science and Technology of China, Suzhou 215123, China. ; 3.Innovation Academy of South China Sea Ecology and Environmental Engineering, Chinese Academy of Sciences, Guangzhou 510301, China. ; Jingyu Li:1.Key Laboratory of Marine Materials and Related Technologies, Zhejiang Key Laboratory of Marine Materials and Protective Technologies, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo 315201, China. ; Jiayu Wei:1.Key Laboratory of Marine Materials and Related Technologies, Zhejiang Key Laboratory of Marine Materials and Protective Technologies, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo
期刊：Copper Tannic Acid-Coordinated Metal–Organic Nanosheets for Synergistic Antimicrobial and Antifouling Coatings
影响因子 ：9.5
引用产品： MCF 10A细胞专用培养基 , DMEM/F12 (含HEPES) 培养基 , 马血清 , 重组人胰岛素溶液 (10mg/mL)
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Inhibition of heat shock protein family A member 8 attenuates spinal cord ischemia–reperfusion injury via astrocyte NF-κB/NLRP3 inflammasome pathway (2021-08-06)
作者：Jingyi Mi:1.Department of Sports Medicine, Wuxi 9Th Affiliated Hospital of Soochow University, Wuxi, 214000, Jiangsu, China. ; Yang Yang:1.Department of Neurosurgery, Central Hospital of Jinzhou, Jinzhou, 121001, Liaoning, China. ; Hao Yao:1.Department of ICU, Wuxi 9Th Affiliated Hospital of Soochow University, Wuxi, 214000, Jiangsu, China. ; Zhirong Huan:1.Department of ICU, Wuxi 9Th Affiliated Hospital of Soochow University, Wuxi, 214000, Jiangsu, China. ; Ce Xu:1.Department of ICU, Wuxi 9Th Affiliated Hospital of Soochow University, Wuxi, 214000, Jiangsu, China. ; Zhiheng Ren:1.Department of Anatomy, School of Medicine, Sun Yat-Sen University, Guangzhou, 510080, Guangdong, China. ; Wenfu Li:1.Department of Anatomy, School of Medicine, Sun Yat-Sen University, Guangzhou, 510080, Guangdong, China. ; Ying Tang:1.Department of Microbiology, Biochemistry, & Molecular Genetics, New Jersey Medical School, Rutgers, The State University of New Jersey, Newark, 07103, NJ, USA. ; 2.Brain
期刊：Inhibition of heat shock protein family A member 8 attenuates spinal cord ischemia–reperfusion injury via astrocyte NF-κB/NLRP3 inflammasome pathway
DOI：10.1186/s12974-021-02220-0
影响因子 ：9.3
引用产品： DMEM/F12 (含HEPES) 培养基
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miR-146a aggravates cognitive impairment and Alzheimer disease-like pathology by triggering oxidative stress through MAPK signaling (2003-01-20)
作者：H Zhan-Qiang:1.Department of General medicine, Affiliated Hospital of Chengde Medical College, Chengde 067000, China. ; Q Hai-Hua:1.Department of Dermatology, Affiliated Hospital of Chengde Medical College, Chengde 067000, China. ; Z Chi:1.Department of Neurology, Affilicated Hospital of Chengde Medical College, Chengde 067000, China. ; W Miao:1.Department of General medicine, Affiliated Hospital of Chengde Medical College, Chengde 067000, China. ; Z Cui:1.Department of General medicine, Affiliated Hospital of Chengde Medical College, Chengde 067000, China. ; L Zi-Yin:1.Department of General medicine, Affiliated Hospital of Chengde Medical College, Chengde 067000, China. ; H Jing:1.Department of General medicine, Affiliated Hospital of Chengde Medical College, Chengde 067000, China. ; W Yi-Wei:1.Department of General medicine, Affiliated Hospital of Chengde Medical College, Chengde 067000, China. Electronic address: wangyiwei9511_cmc@163.com. ;
期刊：miR-146a aggravates cognitive impairment and Alzheimer disease-like pathology by triggering oxidative stress through MAPK signaling
影响因子 ：3.9
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Anti-human fibroblast growth factor-21 monoclonal antibody preparation, characterization and analysis of in vitro bioactivity (2009-06-20)
作者：Liangjun Ding:1.Biopharmaceutical Department, College of Life Science, Northeast Agricultural University, Harbin, Heilongjiang 150030, P.R. China. ; Zhichao Hao:1.State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, Heilongjiang 150069, P.R. China. ; Qingyan Yuan:1.Biopharmaceutical Department, College of Life Science, Northeast Agricultural University, Harbin, Heilongjiang 150030, P.R. China. ; Pengfei Xu:1.Biopharmaceutical Department, College of Life Science, Northeast Agricultural University, Harbin, Heilongjiang 150030, P.R. China. ; Yinhang Yu:1.Biopharmaceutical Department, College of Life Science, Northeast Agricultural University, Harbin, Heilongjiang 150030, P.R. China. ; Deshan Li:1.Biopharmaceutical Department, College of Life Science, Northeast Agricultural University, Harbin, Heilongjiang 150030, P.R. China. ;
期刊：Anti-human fibroblast growth factor-21 monoclonal antibody preparation, characterization and analysis of in vitro bioactivity
影响因子 ：2.7
引用产品： DMEM/F12 (含HEPES) 培养基
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