产品中心Cell Resources
联系我们CONTACT US
400-999-210024小时服务热线
人肺微血管内皮细胞
Human Pulmonary Microvascular Endothelial Cells
货号：CP-H001
规格：5×10⁵Cells/T25培养瓶
价格： ￥7380
培养基货号： CM-H001
产品概述
人肺微血管内皮细胞
Cat NO.: CP-H001
-
产品名称：人肺微血管内皮细胞
-
组织来源：肺组织
-
产品规格：5×10⁵Cells/T25培养瓶
-
细胞简介：
人肺微血管内皮细胞分离自肺组织；肺是机体的呼吸器官，位于胸腔，左右各一，覆盖于心之上。肺有分叶，左二右三，共五叶。肺经肺系(指气管、支气管等)与喉、鼻相连，故称喉为肺之门户，鼻为肺之外窍。微血管内皮细胞密切参与包括再生、发育、伤口愈合等一系列生理及炎症反应。细胞呈梭形或多角形，形成单层后呈鹅卵石样或铺路石样排列。肺微血管内皮细胞构成半选择性屏障，该屏障对于肺气体交换，调节液体和可溶物在血液与肺间质之间的流动具有重要意义。
-
方法简介：
普诺赛实验室分离的人肺微血管内皮细胞采用组织贴块法并结合内皮细胞专用培养基培养筛选制备而来，细胞总量约为5×10⁵cells/瓶。
-
质量检测：
普诺赛实验室分离的人肺微血管内皮细胞经CD31免疫荧光鉴定，纯度可达90%以上，且不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等。
-
培养信息：
包被条件 PLL（0.1mg/ml）或明胶（0.1%） 培养基 含FBS、生长添加剂、Penicillin、Streptomycin等 产品货号 CM-H001 换液频率 每2-3天换液一次 生长特性 贴壁 细胞形态 内皮细胞样 传代特性 可传2-3代 消化液 0.25%胰蛋白酶 人肺微血管内皮细胞体外培养周期有限；建议使用普诺赛配套的专用生长培养基及正确的操作方法来培养，以此保证该细胞的最佳培养状态。
参考文献
-
Carnosol alleviates sepsis-induced pulmonary endothelial barrier dysfunction by targeting nuclear factor erythroid2-related factor 2/sirtuin-3 signaling pathway to attenuate oxidative damage (2024-02-27)
作者：Xingbing Li:1.Department of Cardiology, Chongqing Hospital of Traditional Chinese Medicine, Chongqing, PR China. ; Shuo Wang:1.Department of Cardiology, Daping Hospital, The Third Military Medical University, Chongqing, PR China. ; Minghao Luo:1.Department of Cardiology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, PR China. ; Ming Wang:1.Department of Cardiology, Chongqing Hospital of Traditional Chinese Medicine, Chongqing, PR China. ; Shaoping Wu:1.Department of Cardiology, Chongqing Hospital of Traditional Chinese Medicine, Chongqing, PR China. ; Chang Liu:1.Department of Cardiology, Chongqing Hospital of Traditional Chinese Medicine, Chongqing, PR China. ; Fengxian Wang:1.Department of Cardiology, Daping Hospital, The Third Military Medical University, Chongqing, PR China. ; Yong Li:1.Department of Cardiology, Chongqing Hospital of Traditional Chinese Medicine, Chongqing, PR China. ;
期刊：Carnosol alleviates sepsis-induced pulmonary endothelial barrier dysfunction by targeting nuclear factor erythroid2-related factor 2/sirtuin-3 signaling pathway to attenuate oxidative damage
DOI：10.1002/ptr.8138
影响因子 ：7.2
引用产品： 人肺微血管内皮细胞完全培养基 , 人肺微血管内皮细胞
-
Comprehensive analysis of m6A modification in lipopolysaccharide-induced acute lung injury in mice (2024-01-22)
作者：Chenzhen Xu:1.Department of Thoracic Surgery, Renmin Hospital of Wuhan University, Wuhan, 430060, China. ; Congkuan Song:1.Department of Thoracic Surgery, Renmin Hospital of Wuhan University, Wuhan, 430060, China. ; Wenjie Wang:1.Department of Thoracic Surgery, Renmin Hospital of Wuhan University, Wuhan, 430060, China. ; Bohao Liu:1.Department of Thoracic Surgery, Renmin Hospital of Wuhan University, Wuhan, 430060, China. ; 2.Department of Thoracic Surgery, The First Hospital of Jilin University, Changchun, 130021, China. ; Guorui Li:1.Department of Thoracic Surgery, Renmin Hospital of Wuhan University, Wuhan, 430060, China. ; Tinglv Fu:1.Department of Thoracic Surgery, Renmin Hospital of Wuhan University, Wuhan, 430060, China. ; Bo Hao:1.Department of Thoracic Surgery, Renmin Hospital of Wuhan University, Wuhan, 430060, China. ; Ning Li:1.Department of Thoracic Surgery, Renmin Hospital of Wuhan University, Wuhan, 430060, China. md.lining@whu.edu.cn. ; Qing Geng:1.Department of
期刊：Comprehensive analysis of m6A modification in lipopolysaccharide-induced acute lung injury in mice
DOI：10.1186/s10020-024-00782-2
影响因子 ：5.7
引用产品： 人肺微血管内皮细胞 , 人肺微血管内皮细胞完全培养基
-
Heme-Inducing Endothelial Pyroptosis Plays a Key Role in Radiofrequency Ablation of Hepatic Hemangioma Leading to Systemic Inflammatory Response Syndrome (2024-01-17)
作者：Changyu Yao:1.Department of Hepatobiliary Surgery, Beijing Chaoyang Hospital, Capital Medical University, Beijing, 100043, People's Republic of China. ; Jian Kong:1.Department of Hepatobiliary Surgery, Beijing Chaoyang Hospital, Capital Medical University, Beijing, 100043, People's Republic of China. ; Fei Xu:1.Department of Hepatobiliary Surgery, Beijing Chaoyang Hospital, Capital Medical University, Beijing, 100043, People's Republic of China. ; Shaohong Wang:1.Department of Hepatobiliary Surgery, Beijing Chaoyang Hospital, Capital Medical University, Beijing, 100043, People's Republic of China. ; Shilun Wu:1.Department of Hepatobiliary Surgery, Beijing Chaoyang Hospital, Capital Medical University, Beijing, 100043, People's Republic of China. ; Wenbing Sun:1.Department of Hepatobiliary Surgery, Beijing Chaoyang Hospital, Capital Medical University, Beijing, 100043, People's Republic of China. ; Jun Gao:1.Department of Hepatobiliary Surgery, Beijing Chaoyang Hospital, Capital M
期刊：Heme-Inducing Endothelial Pyroptosis Plays a Key Role in Radiofrequency Ablation of Hepatic Hemangioma Leading to Systemic Inflammatory Response Syndrome
影响因子 ：4.5
引用产品： 人肺微血管内皮细胞
-
Mesenchymal stem cells-derived exosomes ameliorate high glucose and lipopolysaccharide-induced HPMECs injury through the Nrf2/HO-1 pathway (2023-12-31)
作者：Hongyan Sun:1.Department of Endocrine and Metabolic Diseases, Yantaishan Hospital, Yantai, China. ; Shitao Lu:1.Department of Emergency, Yantaishan Hospital, Yantai, China. ; Gaowei Qu:1.Department of Emergency, Yantaishan Hospital, Yantai, China. ; Junli Li:1.Department of Endocrine and Metabolic Diseases, Yantaishan Hospital, Yantai, China. ; Bo Song:1.Department of Emergency, Yantaishan Hospital, Yantai, China. ;
期刊：Mesenchymal stem cells-derived exosomes ameliorate high glucose and lipopolysaccharide-induced HPMECs injury through the Nrf2/HO-1 pathway
DOI：10.1080/08916934.2023.2290357
影响因子 ：3.5
引用产品： 人肺微血管内皮细胞 , 人肺微血管内皮细胞完全培养基 , 人骨髓间充质干细胞 , 人骨髓间充质干细胞完全培养基
-
Polydatin alleviates sepsis‑induced acute lung injury via downregulation of Spi‑B (2023-10-26)
作者：Qingwu Liao:1.Shanghai Key Laboratory of Perioperative Stress and Protection, Department of Anesthesia, Zhongshan Hospital, Fudan University, Shanghai 200032, P.R. China. ; Fang Li:1.Department of Geriatrics, Xiamen Branch, Zhongshan Hospital, Fudan University, Xiamen, Fujian 361015, P.R. China. ; Mingming Xue:1.Department of Emergency, Zhongshan Hospital, Fudan University, Shanghai 200032, P.R. China. ; Wenan Chen:1.Department of Emergency, Zhongshan Hospital, Fudan University, Shanghai 200032, P.R. China. ; Zhengang Tao:1.Department of Emergency, Zhongshan Hospital, Fudan University, Shanghai 200032, P.R. China. ; Yuejiao Song:1.Department of Anesthesia, Xiamen Branch, Zhongshan Hospital, Fudan University, Xiamen, Fujian 361015, P.R. China. ; Ying Yuan:1.Department of Geriatrics, Zhongshan Hospital, Fudan University, Shanghai 200032, P.R. China. ;
期刊：Polydatin alleviates sepsis‑induced acute lung injury via downregulation of Spi‑B
影响因子 ：2.3
引用产品： 人肺微血管内皮细胞
-
CircWDR33 alleviates human pulmonary microvascular endothelial cell injury in sepsis-associated acute lung injury by targeting miR-217-5p/SERP1 axis (2022-11-20)
作者：Ting You:1.The First Affiliated Hospital, Department of Emergency, Hengyang Medical School, University of South China, Hengyang City, Hunan Province 421001, China. ; Bihong Zhang:1.The First Affiliated Hospital, Department of Emergency, Hengyang Medical School, University of South China, Hengyang City, Hunan Province 421001, China. Electronic address: hrpwstk@163.com. ;
期刊：CircWDR33 alleviates human pulmonary microvascular endothelial cell injury in sepsis-associated acute lung injury by targeting miR-217-5p/SERP1 axis
DOI：10.1016/j.intimp.2022.109440
影响因子 ：5.7
-
Silencing long non-coding RNA SNHG3 repairs the dysfunction of pulmonary microvascular endothelial barrier by regulating miR-186-5p/Wnt axis (2022-11-24)
作者：Yanli Wu:1.Department of Critical Care Medicine, Ningxia Medical University, Yinchuan, 750004, Ningxia, PR China; Department of Critical Care Medicine, General Hospital of Ningxia Medical University, Yinchuan, 750004, Ningxia, PR China. ; Mei Li:1.Department of Critical Care Medicine, Ningxia Medical University, Yinchuan, 750004, Ningxia, PR China. ; Jijia Bai:1.Department of Critical Care Medicine, General Hospital of Ningxia Medical University, Yinchuan, 750004, Ningxia, PR China. ; Xigang Ma:1.Department of Critical Care Medicine, General Hospital of Ningxia Medical University, Yinchuan, 750004, Ningxia, PR China. Electronic address: nyfyicu@163.com. ;
期刊：Silencing long non-coding RNA SNHG3 repairs the dysfunction of pulmonary microvascular endothelial barrier by regulating miR-186-5p/Wnt axis
DOI：10.1016/j.bbrc.2022.11.067
影响因子 ：3.3
引用产品： 人肺微血管内皮细胞
FAQs
Q：{{item.question}}
A：
产品资料
识别码示意图
