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人骨髓间充质干细胞
Human Bone Marrow Mesenchymal Stem Cells
货号:CP-H166
规格:5×10⁵Cells/T25培养瓶
价格: ¥7380
培养基货号: CM-H166
产品概述
人骨髓间充质干细胞
Cat NO.: CP-H166
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产品名称:人骨髓间充质干细胞
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英文简称:BMSCs,MSCs
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组织来源:骨髓
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产品规格:5×10⁵Cells/T25培养瓶
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细胞简介:
人骨髓间充质干细胞(BMSC)分离自骨髓;骨髓是机体的造血组织,位于身体的许多骨骼内。成年动物的骨髓分两种:红骨髓和黄骨髓。红骨髓能制造红细胞、血小板和各种白细胞。血小板有止血作用,白细胞能杀灭与抑制各种病原体,包括细菌、病毒等;某些淋巴细胞能制造抗体。因此,骨髓不但是造血器官,它还是重要的免疫器官。骨髓是存在于长骨(如肱骨、股骨)的骨髓腔和扁平骨(如髂骨)的稀松骨质间的网眼中,是一种海绵状的组织,能产生血细胞的骨髓略呈红色,称为红骨髓。出生时,红骨髓充满全身骨髓腔,随着年龄增大,脂肪细胞增多,相当部分红骨髓被黄骨髓取代,最后几乎只有扁平骨骨髓腔中有红骨髓。骨髓基质系统内存在的骨髓间充质干细胞是一种除造血干细胞以外的、具有高度自我更新能力和多向分化潜能的干细胞,可以向骨、软骨、肌组织、皮肤、脂肪、神经等多种组织分化,因此可以作为组织工程中的种子细胞。在骨髓中,BMSC占骨髓有核细胞总数的0.001%-0.1%,含量极低。骨髓间充质干细胞的体外培养条件要求较高,在培养过程中,受贴壁时间、种植密度、血清含量、培养温度和培养基pH值等条件的影响。
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方法简介:
普诺赛实验室分离的人骨髓间充质干细胞采用冲洗骨髓、密度梯度离心、差速贴壁法结合培养基筛选制备而来,细胞总量约为5×10⁵cells/瓶。
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质量检测:
普诺赛实验室分离的人骨髓间充质干细胞经CD29或CD90免疫荧光鉴定,纯度可达90%以上,且不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等。
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培养信息:
包被条件 培养基 含FBS、生长添加剂、Penicillin、Streptomycin等 产品货号 CM-H166 换液频率 每2-3天换液一次 生长特性 贴壁 细胞形态 成纤维细胞样 传代特性 可传3-5代左右;3代以内状态最佳 消化液 0.25%胰蛋白酶 人骨髓间充质干细胞体外培养周期有限;建议使用普诺赛配套的专用生长培养基及正确的操作方法来培养,以此保证该细胞的最佳培养状态。
参考文献
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pH-triggered chitosan-sodium caseinate nanocarriers with charge-switching property: Characterization and applications in dental care (2024-02-23)
期刊:FOOD HYDROCOLLOIDS
DOI:10.1016/j.foodhyd.2024.109919
影响因子 :10.7
引用产品: 人骨髓间充质干细胞
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Bioinformatic analysis of related immune cell infiltration and key genes in the progression of osteonecrosis of the femoral head (2024-01-11)
期刊:Frontiers in Immunology
DOI:10.3389/fimmu.2023.1340446
影响因子 :7.3
引用产品: 人骨髓间充质干细胞
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Trifluoperazine activates AMPK / mTOR / ULK1 signaling pathway to induce mitophagy in osteosarcoma cells (2024-02-13)
期刊:CHEMICO-BIOLOGICAL INTERACTIONS
影响因子 :5.1
引用产品: 143B 细胞 , U-2 OS [U2OS] 细胞 , 人骨髓间充质干细胞
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Bone-targeting exosome nanoparticles activate Keap1 / Nrf2 / GPX4 signaling pathway to induce ferroptosis in osteosarcoma cells (2023-09-30)
期刊:JOURNAL OF NANOBIOTECHNOLOGY
DOI:10.1186/s12951-023-02129-1
影响因子 :10.2
引用产品: 143B 细胞 , MG-63 细胞 , Saos-2 细胞 , U-2 OS [U2OS] 细胞 , 人骨髓间充质干细胞
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Human bone marrow mesenchymal stem cell-derived extracellular vesicles reduce inflammation and pyroptosis in acute kidney injury via miR-223-3p/HDAC2/SNRK (2023-01-14)
期刊:INFLAMMATION RESEARCH
DOI:10.1007/s00011-022-01653-4
影响因子 :6.7
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DZIP1 expressed in fibroblasts and tumor cells may affect immunosuppression and metastatic potential in gastric cancer (2023-02-17)
期刊:INTERNATIONAL IMMUNOPHARMACOLOGY
DOI:10.1016/j.intimp.2023.109886
影响因子 :5.6
引用产品: 人脐带血间充质干细胞
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Inhibitory role of bone marrow mesenchymal stem cells-derived exosome in non-small-cell lung cancer: microRNA-30b-5p, EZH2 and PI3K/AKT pathway (2023-09-12)
期刊:JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
影响因子 :5.3
引用产品: 人骨髓间充质干细胞 , 人骨髓间充质干细胞完全培养基 , 293T [HEK-293T] 细胞 , DMEM高糖 培养基 , 特级胎牛血清 , 青霉素-链霉素溶液 (双抗),100× , A549 [A-549] 细胞 , NCI-H1299 细胞 , Ham's F-12K 培养基
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Injectable EC-BMSC hydrogel with prolonged VEGF action for enhanced angiogenesis (2023-06-21)
期刊:POLYMER TESTING
DOI:10.1016/j.polymertesting.2023.108109
影响因子 :5.1
引用产品: 人骨髓间充质干细胞
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MSC-Derived Exosomes Ameliorate Intervertebral Disc Degeneration By Regulating the Keap1/Nrf2 Axis (2023-08-01)
期刊:Stem Cell Reviews and Reports
DOI:10.1007/s12015-023-10570-w
影响因子 :4.8
引用产品: 人骨髓间充质干细胞
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Incorporating GSE4 peptide in PEG/hyaluronic acid hydrogels to promote the alveolar epithelial differentiation of mesenchymal stem cells (2023-03-09)
期刊:POLYMER
DOI:10.1016/j.polymer.2023.125861
影响因子 :4.6
引用产品: 青霉素-链霉素溶液 (双抗),100× , 人骨髓间充质干细胞 , 人小气道上皮细胞完全培养基
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Mesenchymal stem cell-derived exosomal miR-548x-3p inhibits pyroptosis of vascular endothelial cells through HMGB1 in heat stroke (2023-09-25)
期刊:GENOMICS
DOI:10.1016/j.ygeno.2023.110719
影响因子 :4.4
引用产品: 人骨髓间充质干细胞 , 人骨髓间充质干细胞完全培养基
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Extracellular vesicles derived from hypoxia-preconditioned bone marrow mesenchymal stem cells ameliorate lower limb ischemia by delivering miR-34c (2023-02-02)
期刊:MOLECULAR AND CELLULAR BIOCHEMISTRY
DOI:10.1007/s11010-023-04666-7
影响因子 :4.3
引用产品: 人骨髓间充质干细胞 , THP-1 细胞 , PUMC-HUVEC-T1 细胞 , PUMC-HUVEC-T1细胞专用培养基
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Exosome-mediated transfer of circRNA563 promoting hepatocellular carcinoma by targeting the microRNA148a-3p/metal-regulatory transcription factor-1 pathway (2023-12-14)
期刊:WORLD JOURNAL OF GASTROENTEROLOGY
影响因子 :4.3
引用产品: SNU387 细胞 , 人骨髓间充质干细胞
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GelMA/κ-carrageenan double-network hydrogels with superior mechanics and biocompatibility (2023-01-06)
期刊:RSC Advances
影响因子 :3.9
引用产品: 人骨髓间充质干细胞
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Analysis of the potential biological mechanisms of Danyu Gukang Pill against osteonecrosis of the femoral head based on network pharmacology (2023-01-31)
期刊:BMC Complementary Medicine and Therapies
DOI:10.1186/s12906-023-03843-x
影响因子 :3.9
引用产品: 人骨髓间充质干细胞
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Mesenchymal stem cells-derived exosomes ameliorate high glucose and lipopolysaccharide-induced HPMECs injury through the Nrf2/HO-1 pathway (2023-12-31)
期刊:AUTOIMMUNITY
DOI:10.1080/08916934.2023.2290357
影响因子 :3.5
引用产品: 人肺微血管内皮细胞 , 人肺微血管内皮细胞完全培养基 , 人骨髓间充质干细胞 , 人骨髓间充质干细胞完全培养基
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High glucose levels delay the senescence of stem cells from human exfoliated deciduous teeth by suppressing autophagy (2023-11-17)
期刊:ARCHIVES OF ORAL BIOLOGY
DOI:10.1016/j.archoralbio.2023.105851
影响因子 :3.0
引用产品: 人骨髓间充质干细胞 , DMEM高糖 培养基 , 特级胎牛血清
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Notoginsenoside R1 promotes osteogenic differentiation of human bone marrow mesenchymal stem cells via ERα/GSK-3β/β-catenin signalling pathway (2023-10-30)
期刊:INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY
影响因子 :3.0
引用产品: 人骨髓间充质干细胞
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LncRNA HOTAIRM1 promotes osteogenic differentiation of human bone marrow-derived mesenchymal stem cells by targeting miR-152-3p/ETS1 axis (2023-05-12)
期刊:MOLECULAR BIOLOGY REPORTS
DOI:10.1007/s11033-023-08466-6
影响因子 :2.8
引用产品: 人骨髓间充质干细胞 , 人骨髓间充质干细胞完全培养基
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LINC01234 Sponging of the miR-513a-5p/AOX1 Axis is Upregulated in Osteoporosis and Regulates Osteogenic Differentiation of Bone Marrow Mesenchymal Stem Cells (2023-03-24)
期刊:MOLECULAR BIOTECHNOLOGY
DOI:10.1007/s12033-023-00712-3
影响因子 :2.6
引用产品: 人骨髓间充质干细胞 , 人骨髓间充质干细胞完全培养基
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Ubiquitination of ASCL1 mediates CD47 transcriptional activation of the AKT signaling pathway, and glycolysis promotes osteogenic differentiation of hBMSCs (2023-10-02)
期刊:IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
DOI:10.1007/s11626-023-00811-0
影响因子 :2.1
引用产品: 人骨髓间充质干细胞
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Mesenchymal Stem Cells and Osteoblast Function: Investigating the Involvement of circGLIS2 (2023-12-10)
期刊:RUSSIAN JOURNAL OF GENETICS
影响因子 :0.6
引用产品: 人骨髓间充质干细胞
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Activation of Dynamin-Related Protein 1 and Induction of Mitochondrial Apoptosis by Exosome-Rifampicin Nanoparticles Exerts Anti-Osteosarcoma Effect (2022-11-17)
期刊:International Journal of Nanomedicine
影响因子 :7.0
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CircRNA AFF4 induced by KDM1A promotes osteogenic differentiation through FNDC5/Irisin pathway (2022-11-18)
期刊:MOLECULAR MEDICINE
DOI:10.1186/s10020-022-00557-7
影响因子 :6.4
引用产品: 人骨髓间充质干细胞
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Osteogenesis-Related Long Noncoding RNA GAS5 as a Novel Biomarker for Osteonecrosis of Femoral Head. (2003-02-20)
期刊:Frontiers in Cell and Developmental Biology
影响因子 :5.5
引用产品: 人骨髓间充质干细胞
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Exosomal microRNA-618 derived from mesenchymal stem cells attenuate the progression of hepatic fibrosis by targeting Smad4 (2002-02-20)
期刊:Bioengineered
DOI:10.1080/21655979.2021.2023799
影响因子 :4.9
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Hypoxia pretreatment improves the therapeutic potential of bone marrow mesenchymal stem cells in hindlimb ischemia via upregulation of NRG-1 (2001-02-20)
期刊:Cell And Tissue Research
DOI:10.1007/s00441-021-03562-0
影响因子 :3.6
引用产品: 人骨髓间充质干细胞
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Cimigenol depresses acute myeloid leukemia cells protected by breaking bone marrow stromal cells via CXCR4/SDF‑1α (2022-12-30)
期刊:Experimental and Therapeutic Medicine
影响因子 :2.8
引用产品: 人骨髓间充质干细胞
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Human bone marrow mesenchymal stem cells-derived exosomes protect against nerve injury via regulating immune microenvironment in neonatal hypoxic-ischemic brain damage model (2001-02-20)
期刊:Immunobiology
DOI:10.1016/j.imbio.2022.152178
影响因子 :2.8
引用产品: 人骨髓间充质干细胞
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MiR-145 inhibits the differentiation and proliferation of bone marrow stromal mesenchymal stem cells by GABARAPL1 in steroid-induced femoral head necrosis (2022-11-26)
期刊:BMC MUSCULOSKELETAL DISORDERS
DOI:10.1186/s12891-022-05928-z
影响因子 :2.6
引用产品: 人骨髓间充质干细胞
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LncRNA TUG regulates osteogenic differentiation of bone marrow mesenchymal stem cells via miRNA-204/SIRT 1 (2022-09-01)
期刊:JOURNAL OF MUSCULOSKELETAL & NEURONAL INTERACTIONS
DOI:PMID:36046997
影响因子 :1.9
引用产品: 人骨髓间充质干细胞 , 人骨髓间充质干细胞完全培养基
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Long non-coding RNA DANCR modulates osteogenic differentiation by regulating the miR-1301-3p/PROX1 axis (2002-01-20)
期刊:Molecular And Cellular Biochemistry
DOI:10.1007/s11010-021-04074-9
影响因子 :4.3
引用产品: 人骨髓间充质干细胞 , 293T [HEK-293T] 细胞 , 人骨髓间充质干细胞完全培养基
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Mesenchymal stromal cells can repair radiation-induced pulmonary fibrosis via a DKK-1-mediated Wnt/β-catenin pathway (2001-01-20)
期刊:Cell And Tissue Research
DOI:10.1007/s00441-020-03325-3
影响因子 :3.6
引用产品: 人骨髓间充质干细胞
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Mesenchymal stem cell exosomal tsRNA-21109 alleviate systemic lupus erythematosus by inhibiting macrophage M1 polarization (2008-01-20)
期刊:Molecular Immunology
DOI:10.1016/j.molimm.2021.08.015
影响因子 :3.6
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Long non-coding RNA taurine upregulated gene 1 is downregulated in osteoporosis and influences the osteogenic differentiation of bone marrow mesenchymal stem cells (2004-01-20)
期刊:PeerJ
影响因子 :2.7
引用产品: 人骨髓间充质干细胞 , 人骨髓间充质干细胞完全培养基
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Exosomes derived from TSG-6 modified mesenchymal stromal cells attenuate scar formation during wound healing (2008-00-20)
期刊:Biochimie
DOI:10.1016/j.biochi.2020.08.003
影响因子 :3.9
引用产品: 人骨髓间充质干细胞 , 人脑动脉血管平滑肌细胞完全培养基
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MicroRNA-4461 derived from bone marrow mesenchymal stem cell exosomes inhibits tumorigenesis by downregulating COPB2 expression in colorectal cancer (2010-09-20)
期刊:Bioscience Biotechnology And Biochemistry
DOI:10.1080/09168451.2019.1677452
影响因子 :1.6
引用产品: 人骨髓间充质干细胞
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