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产品概述
大鼠主动脉内皮细胞
Cat NO.: CP-R075
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产品名称：大鼠主动脉内皮细胞
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组织来源：主动脉组织
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产品规格：5×10⁵Cells/T25培养瓶
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细胞简介：
大鼠主动脉内皮细胞分离自主动脉组织；主动脉是体循环的动脉主干。其运行路径为：升主动脉起于左心室，至右侧第2胸肋关节高度移行为主动脉弓，弓行向左后至第4胸椎体下缘移行为降主动脉；在第12胸椎体高度穿膈的主动脉裂孔移行为腹主动脉，以上为胸主动脉，至第4腰椎体下缘分为左、右髂总动脉；髂总动脉在骶髂关节高度分为髂内、外动脉。主动脉内皮细胞是覆盖在主动脉内面的单层细胞，可分泌一系列血管活性物质而保持血管稳态，当其受到炎症或其它因素刺激后稳态被破坏而导致一些心血管疾病的发生。因此，主动脉内皮细胞已成为研究心血管疾病发病机制及治疗药物不可缺少的工具。内皮细胞或血管内皮是一薄层的专门上皮细胞，由一层扁平细胞所组成。它形成血管的内壁，是血管管腔内血液及其他血管壁(单层鳞状上皮)的接口。内皮细胞是沿着整个循环系统，由心脏直至最小的微血管。
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方法简介：
普诺赛实验室分离的大鼠主动脉内皮细胞采用胰蛋白酶-胶原酶联合消化法结合差速贴壁法、并通过内皮细胞专用培养基培养筛选制备而来，细胞总量约为5×10⁵cells/瓶。
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质量检测：
普诺赛实验室分离的大鼠主动脉内皮细胞经CD31免疫荧光鉴定，纯度可达90%以上，且不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等。
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培养信息：
包被条件 PLL（0.1mg/ml）或明胶（0.1%） 培养基 含FBS、生长添加剂、Penicillin、Streptomycin等 产品货号 CM-R075 换液频率 每2-3天换液一次 生长特性 贴壁 细胞形态 内皮细胞样 传代特性 可传2-3代 消化液 0.25%胰蛋白酶 大鼠主动脉内皮细胞体外培养周期有限；建议使用普诺赛配套的专用生长培养基及正确的操作方法来培养，以此保证该细胞的最佳培养状态。
参考文献
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Engineering highly-aligned three-dimensional (3D) cardiac constructs for enhanced myocardial infarction repair (2022-10-27)
作者：Kang Han:1.State Key Laboratory for Manufacturing Systems Engineering, Xi'an Jiaotong University, Xi'an 710049, People's Republic of China. ; 2.NMPA Key Laboratory for Research and Evaluation of Additive Manufacturing Medical Devices, Xi'an Jiaotong University, Xi'an 710049, People's Republic of China. ; Jiankang He:1.State Key Laboratory for Manufacturing Systems Engineering, Xi'an Jiaotong University, Xi'an 710049, People's Republic of China. ; 2.NMPA Key Laboratory for Research and Evaluation of Additive Manufacturing Medical Devices, Xi'an Jiaotong University, Xi'an 710049, People's Republic of China. ; Liyan Fu:1.Department of Physiology and Pathophysiology, Xi'an Jiaotong University School of Basic Medical Sciences, Shaanxi Engineering and Research Center of Vaccine, Key Laboratory of Environment and Genes Related to Diseases of Education Ministry of China, Xi'an 710061, People's Republic of China. ; Mao Mao:1.State Key Laboratory for Manufacturing Systems Engineering, Xi'an J
期刊：Engineering highly-aligned three-dimensional (3D) cardiac constructs for enhanced myocardial infarction repair
影响因子 ：11.1
引用产品： 大鼠主动脉内皮细胞
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Low-Intensity Focused Ultrasound-Responsive Ferrite-Encapsulated Nanoparticles for Atherosclerotic Plaque Neovascularization Theranostics (2008-01-20)
作者：Jianting Yao:1.Department of Ultrasound, Chongqing Key Laboratory of Ultrasound Molecular Imaging, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, 400010, P. R. China. ; Zhuowen Yang:1.Department of Cardiology, The First Affiliated Hospital, Cardiovascular Institute, Harbin Medical University, Harbin, 150001, P. R. China. ; Liandi Huang:1.State Key Laboratory of Ultrasound in Medicine and Engineering, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, 400010, P. R. China. ; Chao Yang:1.Department of Radiology, Chongqing General Hospital, University of Chinese Academy of Sciences, Chongqing, 400014, P. R. China. ; Jianxin Wang:1.Department of Ultrasound, The First Affiliated Hospital of Harbin Medical University, Harbin, 150001, P. R. China. ; Yang Cao:1.Department of Ultrasound, Chongqing Key Laboratory of Ultrasound Molecular Imaging, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, 400010, P. R. China.
期刊：Low-Intensity Focused Ultrasound-Responsive Ferrite-Encapsulated Nanoparticles for Atherosclerotic Plaque Neovascularization Theranostics
影响因子 ：15.1
引用产品： 大鼠主动脉内皮细胞
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miR‐448‐3p alleviates diabetic vascular dysfunction by inhibiting endothelial–mesenchymal transition through DPP‐4 dysregulation (2006-00-20)
作者：Guo-Ying Guan:1.Department of Geriatrics, The First Affiliated Hospital of Harbin Medical University, Harbin, China. ; Nan Wei:1.Department of Geriatrics, The First Affiliated Hospital of Harbin Medical University, Harbin, China. ; Tao Song:1.Department of Geriatrics, The First Affiliated Hospital of Harbin Medical University, Harbin, China. ; Chao Zhao:1.Department of Geriatrics, The First Affiliated Hospital of Harbin Medical University, Harbin, China. ; Yang Sun:1.Department of Geriatrics, The First Affiliated Hospital of Harbin Medical University, Harbin, China. ; Ru-Xin Pan:1.Department of Geriatrics, The First Affiliated Hospital of Harbin Medical University, Harbin, China. ; Lu-Lu Zhang:1.Department of Geriatrics, The First Affiliated Hospital of Harbin Medical University, Harbin, China. ; Ying-Ying Xu:1.Department of Geriatrics, The First Affiliated Hospital of Harbin Medical University, Harbin, China. ; Ya-Mei Dai:1.Physical Examination Center, The First Affiliated Hos
期刊：miR‐448‐3p alleviates diabetic vascular dysfunction by inhibiting endothelial–mesenchymal transition through DPP‐4 dysregulation
影响因子 ：5.6
引用产品： 大鼠主动脉内皮细胞
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