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产品概述
大鼠真皮成纤维细胞
Cat NO.: CP-R086
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产品名称：大鼠真皮成纤维细胞
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组织来源：皮肤组织
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产品规格：5×10⁵Cells/T25培养瓶
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细胞简介：
大鼠真皮成纤维细胞分离自真皮组织；皮肤组织来源包括头部、脸部、手、脚、腿、背部、腹部、包皮等体表部位皮肤，可依据需求选用；真皮，位于表皮深层，向下与皮下组织相连，真皮结缔组织的胶原纤维和弹性纤维互相交织在一起，埋于基质内。其内分布着各种结缔组织细胞和大量的胶原纤维弹性纤维，使皮肤既有弹性，又有韧性。其由两层组成——乳头层与网状层。真皮的结构组成是胶原蛋白、弹性纤维以及基质。成纤维细胞(Fibroblast)是疏松结缔组织的主要细胞成分，由胚胎时期的间充质细胞分化而来。成纤维细胞较大，轮廓清楚，多为突起的纺锤形或星形的扁平状结构，其细胞核呈规则的卵圆形，核仁大而明显。成纤维细胞功能活动旺盛，细胞质嗜弱碱性，具明显的蛋白质合成和分泌活动，在一定条件下，它可以实现跟纤维细胞的互相转化；成纤维细胞对不同程度的细胞变性、坏死和组织缺损的修复有着十分重要的作用。刚分离的真皮成纤维细胞呈圆形、折光性良好，悬浮于培养基中。30min细胞贴壁，其中部分开始伸出伪足，表现为小的突起；6h后细胞基本贴壁完全，伸展成梭形，胞核清晰，分布较均匀，散在生长，不聚集成团；细胞生长迅速，5-7天即呈融合状态，细胞排列紧密，有的交叉重叠生长，平坦、胞体较大，细胞质透明，细胞核较大，呈椭圆形，颜色淡。细胞融合，并彼此连接成网状；细胞呈突起的纺锤形或星形的扁平分布。真皮成纤维细胞在生理条件下的主要功能包括：构造和维持组织的正常形态，合成和释放细胞外基质以及组织损伤后及时大量聚集修复损伤组织。
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方法简介：
普诺赛实验室分离的大鼠真皮成纤维细胞采用先中性蛋白酶消化、后胰蛋白酶-胶原酶混合消化法结合差速贴壁法制备而来，细胞总量约为5×10⁵cells/瓶。
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质量检测：
普诺赛实验室分离的大鼠真皮成纤维细胞经Vimentin免疫荧光鉴定，纯度可达90%以上，且不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等。
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培养信息：
包被条件 培养基 含FBS、生长添加剂、Penicillin、Streptomycin等 产品货号 CM-R086 换液频率 每2-3天换液一次 生长特性 贴壁 细胞形态 成纤维细胞样 传代特性 可传5代左右；3代以内状态最佳 消化液 0.25%胰蛋白酶 大鼠真皮成纤维细胞体外培养周期有限；建议使用普诺赛配套的专用生长培养基及正确的操作方法来培养，以此保证该细胞的最佳培养状态。
参考文献
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Silencing of topical proline hydroxylase domain 2 promotes the healing of rat diabetic wounds by phosphorylating AMPK (2023-12-01)
作者：Defu Xie:1.Southwest Medical University, No. 1 Section 1, Xianglin Road, Luzhou City, Sichuan Province, 646000, China. ; 2.National Key Clinical Construction Specialty, Wound Repair & Regeneration Laboratory, Department of Plastic & Burn Surgery, Affiliated Hospital of Southwest Medical University, No. 25 Taiping Street, Jiangyang District, Luzhou,Sichuan Province, 646000, China. ; Mengchang Liu:1.Southwest Medical University, No. 1 Section 1, Xianglin Road, Luzhou City, Sichuan Province, 646000, China. ; 2.National Key Clinical Construction Specialty, Wound Repair & Regeneration Laboratory, Department of Plastic & Burn Surgery, Affiliated Hospital of Southwest Medical University, No. 25 Taiping Street, Jiangyang District, Luzhou,Sichuan Province, 646000, China. ; Yingxi Lin:1.Southwest Medical University, No. 1 Section 1, Xianglin Road, Luzhou City, Sichuan Province, 646000, China. ; 2.National Key Clinical Construction Specialty, Wound Repair & Regeneration Laboratory, Department o
期刊：Silencing of topical proline hydroxylase domain 2 promotes the healing of rat diabetic wounds by phosphorylating AMPK
DOI：10.1371/journal.pone.0294566
影响因子 ：3.7
引用产品： 大鼠真皮成纤维细胞
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Rapamycin incorporating hydrogel improves the progression of osteoarthritis by inducing synovial macrophages polarization and reducing intra-articular inflammation (2022-12-24)
作者：Bo Chen: 1.Rehabilitation Medicine Department, Northern Jiangsu People’s Hospital, Yangzhou, Jiangsu 225001, China; Jibing Wang: 1.Rehabilitation Medicine Department, Northern Jiangsu People’s Hospital, Yangzhou, Jiangsu 225001, China; Xing Jin: 1.Rehabilitation Medicine Department, Northern Jiangsu People’s Hospital, Yangzhou, Jiangsu 225001, China; Xin Wang: 1.Rehabilitation Medicine Department, Northern Jiangsu People’s Hospital, Yangzhou, Jiangsu 225001, China; Zhangling Fan: 1.Rehabilitation Medicine Department, Northern Jiangsu People’s Hospital, Yangzhou, Jiangsu 225001, China; Yuxin Zhang: 1.Department of Oral Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology, Shanghai 200011, China; Xuehan Sang: 1.Rehabilitation Medicine Department, Northern Jiangsu People’s
期刊：Rapamycin incorporating hydrogel improves the progression of osteoarthritis by inducing synovial macrophages polarization and reducing intra-articular inflammation
DOI：10.1016/j.matdes.2022.111542
影响因子 ：9.4
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