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人牙周膜成纤维细胞
Human Periodontal Ligament Fibroblast Cells
货号：CP-H136
规格：5×10⁵Cells/T25培养瓶
价格： ￥5880
培养基货号： CM-H136
产品概述
人牙周膜成纤维细胞
Cat NO.: CP-H136
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产品名称：人牙周膜成纤维细胞
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英文简称：PDLFs
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组织来源：牙周膜组织
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产品规格：5×10⁵Cells/T25培养瓶
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细胞简介：
人牙周膜成纤维细胞分离自牙周膜组织；牙周膜(牙周韧带、牙周间隙)是由致密结缔组织所构成。多数纤维排列成束，纤维的一端埋于牙骨质内，另一端则埋于牙槽窝骨壁里，使牙齿固位于牙槽窝内；牙周膜内有神经、血管、淋巴和上皮细胞等。成纤维细胞(Fibroblast)是疏松结缔组织的主要细胞成分，由胚胎时期的间充质细胞分化而来。成纤维细胞较大，轮廓清楚，多为突起的纺锤形或星形的扁平状结构，其细胞核呈规则的卵圆形，核仁大而明显。成纤维细胞功能活动旺盛，细胞质嗜弱碱性，具明显的蛋白质合成和分泌活动，在一定条件下，它可以实现跟纤维细胞的互相转化；成纤维细胞对不同程度的细胞变性、坏死和组织缺损的修复有着十分重要的作用。刚分离的牙周膜成纤维细胞呈圆形、折光性良好，悬浮于培养基中。30min细胞贴壁，其中部分开始伸出伪足，表现为小的突起；6h后细胞基本贴壁完全，伸展成梭形，胞核清晰，分布较均匀，散在生长，不聚集成团；细胞生长迅速，5-7天即呈融合状态，细胞排列紧密，有的交叉重叠生长，平坦、胞体较大，细胞质透明，细胞核较大，呈椭圆形，颜色淡。细胞融合，并彼此连接成网状；细胞呈突起的纺锤形或星形的扁平分布。牙周膜成纤维细胞(PLFs)作为牙周膜的主体细胞，是牙周膜主要的间质细胞，它不仅具有合成胶原、基质、弹力纤维和糖蛋白的功能，还有吸收胶原吞噬异物的能力，还参与了牙周组织的病变、修复及再生过程。现在，利用牙周膜细胞建立体外模型，已经成为有关员研究牙周组织疾病的重要手段。
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方法简介：
普诺赛实验室分离的人牙周膜成纤维细胞采用胶原酶 - 胰蛋白酶联合消化法制备而来，细胞总量约为5×10⁵cells/瓶。
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质量检测：
普诺赛实验室分离的人牙周膜成纤维细胞经Vimentin免疫荧光鉴定，纯度可达90%以上，且不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等。
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培养信息：
包被条件 培养基 含FBS、生长添加剂、Penicillin、Streptomycin等 产品货号 CM-H136 换液频率 每2-3天换液一次 生长特性 贴壁 细胞形态 成纤维细胞样 传代特性 可传5代左右；3代以内状态最佳 消化液 0.25%胰蛋白酶 人牙周膜成纤维细胞体外培养周期有限；建议使用普诺赛配套的专用生长培养基及正确的操作方法来培养，以此保证该细胞的最佳培养状态。
参考文献
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An injectable and coagulation-independent Tetra-PEG hydrogel bioadhesive for post-extraction hemostasis and alveolar bone regeneration (2024-03-19)
作者：Gang He: 1.Guangdong Provincial Key Laboratory of Advanced Biomaterials, Department of Biomedical Engineering, Southern University of Science and Technology, No. 1088 Xueyuan Avenue, Nanshan District, Shenzhen, Guangdong, 518055, China; Yiwen Xian: 1.Guangdong Provincial Key Laboratory of Advanced Biomaterials, Department of Biomedical Engineering, Southern University of Science and Technology, No. 1088 Xueyuan Avenue, Nanshan District, Shenzhen, Guangdong, 518055, China; Huajun Lin: 1.Department of Maxillofacial Surgery, Shenzhen Hospital, Southern Medical University, No. 1333 New Road, Baoan District, Shenzhen, Guangdong, 518101, China; Chengcheng Yu: 1.Department of Maxillofacial Surgery, Shenzhen Hospital, Southern Medical University, No. 1333 New Road, Baoan District, Shenzhen, Guangdong, 518101, China; Luyuan Chen: 1.Department of Maxillofacial Surgery, Shenzhen Hospital, Southern Medical University, No. 1333 New Road, Baoan District, Shenzhen, Guangdong, 518101, China; Zhihui Ch
期刊：An injectable and coagulation-independent Tetra-PEG hydrogel bioadhesive for post-extraction hemostasis and alveolar bone regeneration
DOI：10.1016/j.bioactmat.2024.03.015
影响因子 ：18.9
引用产品： 人牙周膜成纤维细胞 , 大鼠骨髓间充质干细胞
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Bismuth Quantum Dot (Bi QD)/Polydimethylsiloxane (PDMS) Nanocomposites with Self-Cleaning and Antibacterial Activity for Dental Applications (2022-11-05)
作者：Yingzi Hu:1.Department of Stomatology, Affiliated Hospital of Nantong University, 20 Xisi Road, Nantong 226001, China. ; Zhiliang Xu:1.Department of Stomatology, Affiliated Hospital of Nantong University, 20 Xisi Road, Nantong 226001, China. ; Yi Hu:1.School of Chemistry and Chemical Engineering, Nantong University, Nantong 226019, China. ; Lanping Hu:1.School of Chemistry and Chemical Engineering, Nantong University, Nantong 226019, China. ; You Zi:1.School of Chemistry and Chemical Engineering, Nantong University, Nantong 226019, China. ; Mengke Wang:1.School of Chemistry and Chemical Engineering, Nantong University, Nantong 226019, China. ; Xingmei Feng:1.Department of Stomatology, Affiliated Hospital of Nantong University, 20 Xisi Road, Nantong 226001, China. ; Weichun Huang:1.School of Chemistry and Chemical Engineering, Nantong University, Nantong 226019, China. ;
期刊：Bismuth Quantum Dot (Bi QD)/Polydimethylsiloxane (PDMS) Nanocomposites with Self-Cleaning and Antibacterial Activity for Dental Applications
影响因子 ：5.7
引用产品： 人牙周膜成纤维细胞
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Hsa_circ_0099630 knockdown induces the proliferation and osteogenic differentiation and attenuates the apoptosis of porphyromonas gingivalis lipopolysaccharide-induced human periodontal ligament fibroblasts. (2022-09-01)
作者：Yaru Wei:1.Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Stomatology, Guangzhou, China. ; Zhengjun Peng:1.Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Stomatology, Guangzhou, China. ;
期刊：Hsa_circ_0099630 knockdown induces the proliferation and osteogenic differentiation and attenuates the apoptosis of porphyromonas gingivalis lipopolysaccharide-induced human periodontal ligament fibroblasts.
影响因子 ：3.6
引用产品： 人牙周膜成纤维细胞
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