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产品概述
人关节软骨细胞
Cat NO.: CP-H096
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产品名称：人关节软骨细胞
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组织来源：关节软骨组织
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产品规格：5×10⁵Cells/T25培养瓶
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细胞简介：
人关节软骨细胞分离自关节软骨组织；关节软骨属于透明软骨，表面光滑、呈淡蓝色、有光泽，它是由一种特殊的叫做致密结缔组织的胶原纤维构成的基本框架，这种框架呈半环形，类似拱形球门，其底端紧紧附着在下面的骨质上，上端朝向关节面，这种结构使关节软骨紧紧与骨结合起来而不会掉下来，同时当受到压力时候，还可以有少许的变形，起到缓冲压力的作用。在这些纤维之间，散在分布着软骨细胞，软骨细胞由浅层向深层逐渐由扁平样至椭圆或圆形的细胞组成，这些软骨细胞维持关节软骨的正常代谢。关节软骨没有神经支配，也没有血管，其营养成分必须从关节液中取得，而其代谢废物也必须排至关节液中，关节软骨的这种营养代谢必须通过关节运动，使关节软骨不断的受到压力刺激才行，所以关节运动对于维持关节软骨的正常结构起重要的作用。关节软骨细胞位于关节软骨陷窝内。幼稚的关节软骨细胞位于关节软骨组织的表层，单个分布、体积较小、呈椭圆形，长轴与关节软骨表面平行，越向深层的关节软骨细胞体积之间增大呈圆形，细胞核圆形或卵圆形、染色浅，细胞质弱嗜碱性，常见数量不一的脂滴。成熟的关节软骨细胞多2-8个成群分布于关节软骨陷窝内，这些关节软骨细胞由同一个母细胞分裂增殖而成，称为同源细胞群。电镜下，关节软骨细胞有突起和皱褶，细胞质内有大量的粗面内质网和发达的高尔基复合体及少量的线粒体。在组织切片中，关节软骨细胞收缩为不规则形，在软骨囊和细胞之间出现较大的腔隙。体外培养的关节软骨细胞对于研究其生理功能、药物作用以及各种致病因素作用下的病理生理改变具重要意义。
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方法简介：
普诺赛实验室分离的人关节软骨细胞采用胶原酶联合中性蛋白酶消化制备而来，细胞总量约为5×10⁵cells/瓶。
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质量检测：
普诺赛实验室分离的人关节软骨细胞经Ⅱ型胶原蛋白免疫荧光鉴定，纯度可达90%以上，且不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等。
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培养信息：
包被条件 培养基 含FBS、生长添加剂、Penicillin、Streptomycin等 产品货号 CM-H096 换液频率 每3-4天换液一次 生长特性 贴壁 细胞形态 梭形、多角形 传代特性 可传5代左右；3代以内状态最佳 消化液 0.25%胰蛋白酶 人关节软骨细胞体外培养周期有限；建议使用普诺赛配套的专用生长培养基及正确的操作方法来培养，以此保证该细胞的最佳培养状态。
参考文献
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1,25-Dihydroxyvitamin D Deficiency Accelerates Aging-related Osteoarthritis via Downregulation of Sirt1 in Mice (2023-01-01)
作者：Jie Chen:1.The Research Center for Bone and Stem Cells, Department of Anatomy, Histology and Embryology, Nanjing Medical University, Nanjing, China. ; 2.Center for Experimental Medicine, The Third Xiangya Hospital, Central South University, Changsha, China. ; Jiao Zhang:1.Department of Plastic Surgery, Affiliated Friendship Plastic Surgery Hospital of Nanjing Medical University, Nanjing Medical University, Nanjing, China. ; 2.The Research Center for Bone and Stem Cells, Department of Anatomy, Histology and Embryology, Nanjing Medical University, Nanjing, China. ; Jie Li:1.The Research Center for Bone and Stem Cells, Department of Anatomy, Histology and Embryology, Nanjing Medical University, Nanjing, China. ; Ran Qin:1.The Research Center for Bone and Stem Cells, Department of Anatomy, Histology and Embryology, Nanjing Medical University, Nanjing, China. ; Na Lu:1.The Research Center for Bone and Stem Cells, Department of Anatomy, Histology and Embryology, Nanjing Medical Universit
期刊：1,25-Dihydroxyvitamin D Deficiency Accelerates Aging-related Osteoarthritis via Downregulation of Sirt1 in Mice
影响因子 ：9.2
引用产品： 人关节软骨细胞
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RNF125, transcriptionally regulated by NFATC2, alleviates osteoarthritis via inhibiting the Wnt/β-catenin signaling pathway through degrading TRIM14 (2023-11-09)
作者：Runxiao Lv:1.Department of Rehabilitation Medicine, Shengjing Hospital of China Medical University, Shenyang, Liaoning Province, PR China. ; Lili Du:1.Department of Pathophysiology, College of Basic Medical Science, China Medical University, Shenyang, Liaoning Province, PR China. ; Lunhao Bai:1.Department of Rehabilitation Medicine, Shengjing Hospital of China Medical University, Shenyang, Liaoning Province, PR China. Electronic address: blh_ortho@163.com. ;
期刊：RNF125, transcriptionally regulated by NFATC2, alleviates osteoarthritis via inhibiting the Wnt/β-catenin signaling pathway through degrading TRIM14
DOI：10.1016/j.intimp.2023.111191
影响因子 ：5.6
引用产品： 人关节软骨细胞 , 人关节软骨细胞完全培养基
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Analysis of the mechanism of curcumin against osteoarthritis using metabolomics and transcriptomics (2023-11-08)
作者：Wenxiang Deng:1.College of Integrated Traditional Chinese and Western Medicine, Hunan University of Chinese Medicine, Changsha, 410208, Hunan, China. ; Qinghu He:1.Department of Rehabilitation and Healthcare, Hunan University of Medicine, Huaihua, 418000, Hunan, China. hqh19651111@dingtalk.com. ; 2.College of Integrated Traditional Chinese and Western Medicine, Hunan University of Chinese Medicine, Changsha, 410208, Hunan, China. hqh19651111@dingtalk.com. ; Wenan Zhang:1.College of Integrated Traditional Chinese and Western Medicine, Hunan University of Chinese Medicine, Changsha, 410208, Hunan, China. ;
期刊：Analysis of the mechanism of curcumin against osteoarthritis using metabolomics and transcriptomics
DOI：10.1007/s00210-023-02785-y
影响因子 ：3.6
引用产品： 人关节软骨细胞 , 人关节软骨细胞完全培养基 , 0.25%胰蛋白酶溶液 (溶于PBS)
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Study on the mechanism of puerarin against osteoarthritis from ferroptosis based on network pharmacology and bioinformatics (2023-08-07)
作者：Wenxiang Deng:1.College of Integrated Traditional Chinese and Western Medicine, Hunan University of Chinese Medicine, Changsha, 410208, Hunan, China. ; Wenan Zhang:1.College of Integrated Traditional Chinese and Western Medicine, Hunan University of Chinese Medicine, Changsha, 410208, Hunan, China. ; Qinghu He:1.College of Integrated Traditional Chinese and Western Medicine, Hunan University of Chinese Medicine, Changsha, 410208, Hunan, China. hqh19651111@dingtalk.com. ; 2.Department of Rehabilitation and Healthcare, Hunan University of Medicine, Huaihua, 418000, Hunan, China. hqh19651111@dingtalk.com. ;
期刊：Study on the mechanism of puerarin against osteoarthritis from ferroptosis based on network pharmacology and bioinformatics
DOI：10.1007/s00210-023-02653-9
影响因子 ：3.6
引用产品： 人关节软骨细胞 , 人关节软骨细胞完全培养基 , 0.25%胰蛋白酶溶液 (含EDTA，溶于PBS) , 磷酸盐缓冲液 (PBS) 1×
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Adipose mesenchymal stem cells-derived exosomes alleviate osteoarthritis by transporting microRNA -376c-3p and targeting the WNT-beta-catenin signaling axis (2022-11-17)
作者：Feng Li:1.Department of Orthopedics, The First Affiliated Hospital of Zhengzhou University, No. 1, Jianshe East Road, Zhengzhou, 450052, Henan, People's Republic of China. Fengli_jea@163.com. ; Zhiming Xu:1.Department of Orthopedics, The First Affiliated Hospital of Zhengzhou University, No. 1, Jianshe East Road, Zhengzhou, 450052, Henan, People's Republic of China. ; Zheng Xie:1.Department of Orthopedics, The First Affiliated Hospital of Zhengzhou University, No. 1, Jianshe East Road, Zhengzhou, 450052, Henan, People's Republic of China. ; Xing Sun:1.Department of Orthopedics, The First Affiliated Hospital of Zhengzhou University, No. 1, Jianshe East Road, Zhengzhou, 450052, Henan, People's Republic of China. ; Chengxiang Li:1.Department of Orthopedics, The First Affiliated Hospital of Zhengzhou University, No. 1, Jianshe East Road, Zhengzhou, 450052, Henan, People's Republic of China. ; Yangyang Chen:1.Department of Orthopedics, The First Affiliated Hospital of Zhengzhou Univers
期刊：Adipose mesenchymal stem cells-derived exosomes alleviate osteoarthritis by transporting microRNA -376c-3p and targeting the WNT-beta-catenin signaling axis
DOI：10.1007/s10495-022-01787-0
影响因子 ：5.6
引用产品： 人关节软骨细胞 , 人关节软骨细胞完全培养基
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ERα/β/DMP1 axis promotes trans-differentiation of chondrocytes to bone cells through GSK-3β/β-catenin pathway (2001-02-20)
作者：Xue Yan:1.Department of Pediatrics, The Second Hospital of Hebei Medical University, Shijiazhuang, China. ; Deng-Yun Fan:1.Department of Pediatrics, The Second Hospital of Hebei Medical University, Shijiazhuang, China. ; Ya-Lei Pi:1.Department of Pediatrics, The Second Hospital of Hebei Medical University, Shijiazhuang, China. ; Ya-Nan Zhang:1.Department of Pediatrics, The Second Hospital of Hebei Medical University, Shijiazhuang, China. ; Peng-Jiu Fu:1.Department of Pediatrics, The Second Hospital of Hebei Medical University, Shijiazhuang, China. ; Hui-Feng Zhang:1.Department of Pediatrics, The Second Hospital of Hebei Medical University, Shijiazhuang, China. ;
期刊：ERα/β/DMP1 axis promotes trans-differentiation of chondrocytes to bone cells through GSK-3β/β-catenin pathway
影响因子 ：2.9
引用产品： 人关节软骨细胞
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Effect of DNA methylation on gene transcription is associated with the distribution of methylation sites across the genome in osteoarthritis (2003-01-20)
作者：Peng Yi:1.Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China. ; Xiongfeng Xu:1.Department of Emergency, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China. ; Jiawei Yao:1.Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China. ; Bo Qiu:1.Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China. ;
期刊：Effect of DNA methylation on gene transcription is associated with the distribution of methylation sites across the genome in osteoarthritis
影响因子 ：2.7
引用产品： 人关节软骨细胞
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The use of mechano growth factor to prevent cartilage degeneration in knee osteoarthritis (2006-07-20)
作者：Yang Song:1.111 Project Laboratory of Biomechanics and Tissue Repair, Bioengineering College, Chongqing University, Chongqing, China. ; 2.Biosystems and Biomaterials Division, National Institute of Standards and Technology, Gaithersburg, MD, USA. ; Kang Xu:1.111 Project Laboratory of Biomechanics and Tissue Repair, Bioengineering College, Chongqing University, Chongqing, China. ; 2.Department of Bioengineering, University of California, Berkeley, Berkeley, CA, USA. ; Can Yu:1.111 Project Laboratory of Biomechanics and Tissue Repair, Bioengineering College, Chongqing University, Chongqing, China. ; Lili Dong:1.111 Project Laboratory of Biomechanics and Tissue Repair, Bioengineering College, Chongqing University, Chongqing, China. ; Peixing Chen:1.111 Project Laboratory of Biomechanics and Tissue Repair, Bioengineering College, Chongqing University, Chongqing, China. ; Yonggang Lv:1.111 Project Laboratory of Biomechanics and Tissue Repair, Bioengineering College, Chongqing Universit
期刊：The use of mechano growth factor to prevent cartilage degeneration in knee osteoarthritis
影响因子 ：3.3
引用产品： 人关节软骨细胞
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