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产品概述
人滑膜细胞
Cat NO.: CP-H094
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产品名称：人滑膜细胞
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组织来源：滑膜组织
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产品规格：5×10⁵Cells/T25培养瓶
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细胞简介：
人滑膜细胞分离自滑膜组织；滑膜组织是位于关节腔内面的内衬结构，各种关节内疾病均会累及滑膜。而滑膜细胞是维持关节正常功能的重要组织结构，同时在各种关节疾患中也是主要病变部位。骨关节炎(OA)以关节软骨退行性变为特征，其病理改变累及关节的各个组成部分，但绝不仅局限于软骨，还包括软骨下骨、滑膜、半月板和韧带。各组成部分的病理改变相互影响，相互作用，共同加速关节的退变。滑膜细胞是构成滑膜层的最大细胞群体，是维持关节正常功能的重要组织结构，它包埋在颗粒状无定性的基质中，基质内有分散的纤维分布。滑膜由A型(巨噬样滑膜细胞)、B型(成纤维样滑膜细胞)以及C型(树突细胞样滑膜细胞)细胞组成。滑膜细胞主要功能：①滑膜细胞产生润滑液成分，并且与关节腔的吸收和血液/润滑液交换有关；②滑膜细胞增生，表现为不依赖于支持物生长，并且分泌大量的效应分子来促进炎症和关节损坏；③是自身自分泌和旁分泌网络中效应因子的一部分。
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方法简介：
普诺赛实验室分离的人滑膜细胞采用混合酶消化法制备而来，细胞总量约为5×10⁵cells/瓶。
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质量检测：
普诺赛实验室分离的人滑膜细胞经Vimentin免疫荧光鉴定，纯度可达90%以上，且不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等。
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培养信息：
包被条件 培养基 含FBS、生长添加剂、Penicillin、Streptomycin等 产品货号 CM-H094 换液频率 每2-3天换液一次 生长特性 贴壁 细胞形态 成纤维细胞样 传代特性 可传3-5代左右 消化液 0.25%胰蛋白酶 人滑膜细胞体外培养周期有限；建议使用普诺赛配套的专用生长培养基及正确的操作方法来培养，以此保证该细胞的最佳培养状态。
参考文献
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Verification of cuproptosis-related diagnostic model associated with immune infiltration in rheumatoid arthritis (2023-07-20)
作者：Mingyang Jiang:1.Department of Bone and Joint Surgery, Guangxi Medical University First Affiliated Hospital, Nanning, China. ; Kaicheng Liu:1.Department of Bone and Joint Surgery, Guangxi Medical University First Affiliated Hospital, Nanning, China. ; Shenyi Lu:1.Department of Rehabilitation, The Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, China. ; Yue Qiu:1.Department of Bone and Joint Surgery, Guangxi Medical University First Affiliated Hospital, Nanning, China. ; Xiaochong Zou:1.Department of Bone and Joint Surgery, Guangxi Medical University First Affiliated Hospital, Nanning, China. ; Ke Zhang:1.Department of Bone and Joint Surgery, Guangxi Medical University First Affiliated Hospital, Nanning, China. ; Chuanliang Chen:1.Department of Bone and Joint Surgery, Guangxi Medical University First Affiliated Hospital, Nanning, China. ; Yiji Jike:1.Department of Bone and Joint Surgery, Guangxi Medical University First Affiliated Hospital, Nanning, C
期刊：Verification of cuproptosis-related diagnostic model associated with immune infiltration in rheumatoid arthritis
DOI：10.3389/fendo.2023.1204926
影响因子 ：5.2
引用产品： 人滑膜成纤维细胞 (类风湿关节炎) , 人滑膜细胞
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Programmed cell death 10 can be used as a potential biomarker for ankylosing spondylitis diagnosis and treatment (2023-12-29)
作者：Wen-Juan Ni:1.Key Laboratory of Prevention and Treatment of Cardiovascular and Cerebrovascular of Ministry of Education, Gannan Medical University, Ganzhou, 341000, Jiangxi, People's Republic of China. ; 2.School of Basic Medicine, Gannan Medical University, Ganzhou, 341000, Jiangxi, People's Republic of China. ; Xiao-Min Leng:1.Key Laboratory of Prevention and Treatment of Cardiovascular and Cerebrovascular of Ministry of Education, Gannan Medical University, Ganzhou, 341000, Jiangxi, People's Republic of China. lengxm@mail2.sysu.edu.cn. ; 2.School of Basic Medicine, Gannan Medical University, Ganzhou, 341000, Jiangxi, People's Republic of China. lengxm@mail2.sysu.edu.cn. ; 3.The First Affiliated Hospital of Xinxiang Medical University, Xinxiang, 453100, Henan, People's Republic of China. lengxm@mail2.sysu.edu.cn. ;
期刊：Programmed cell death 10 can be used as a potential biomarker for ankylosing spondylitis diagnosis and treatment
DOI：10.1038/s41393-023-00952-9
影响因子 ：2.2
引用产品： 人滑膜细胞 , 人滑膜细胞完全培养基
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LncRNA TM1-3P Regulates Proliferation, Apoptosis and Inflammation of Fibroblasts in Osteoarthritis through miR-144-3p/ONECUT2 Axis (2022-09-30)
作者：Yangfei Yi:1.Department of Clinical Medicine, School of Medicine, Hunan Normal University, Changsha, China. ; Ningyin Yang:1.Department of Clinical Medicine, School of Medicine, Hunan Normal University, Changsha, China. ; Zirui Yang:1.Department of Clinical Medicine, School of Medicine, Hunan Normal University, Changsha, China. ; Xiaojun Tao:1.Key Laboratory of Study and Discovery of Small Targeted Molecules of Hunan Province, School of Medicine, Hunan Normal University, Changsha, China. ; Yufei Li:1.Department of Clinical Medicine, School of Medicine, Hunan Normal University, Changsha, China. ;
期刊：LncRNA TM1-3P Regulates Proliferation, Apoptosis and Inflammation of Fibroblasts in Osteoarthritis through miR-144-3p/ONECUT2 Axis
DOI：10.1111/os.13530
影响因子 ：2.3
引用产品： 人滑膜细胞
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Targeting FSTL1 for Multiple Fibrotic and Systemic Autoimmune Diseases (2009-00-20)
作者：Xiaohe Li:1.State Key Laboratory of Medicinal Chemical Biology, College of Life Sciences, College of Pharmacy, Tianjin Key Laboratory of Protein Sciences, Nankai University, Tianjin 300071, China. ; Yinshan Fang:1.State Key Laboratory of Medicinal Chemical Biology, College of Life Sciences, College of Pharmacy, Tianjin Key Laboratory of Protein Sciences, Nankai University, Tianjin 300071, China. ; Dingyuan Jiang:1.Department of Pulmonary and Critical Care Medicine, China-Japan Friendship Hospital, Beijing 100029, China. ; Yingying Dong:1.State Key Laboratory of Medicinal Chemical Biology, College of Life Sciences, College of Pharmacy, Tianjin Key Laboratory of Protein Sciences, Nankai University, Tianjin 300071, China. ; Yingying Liu:1.State Key Laboratory of Medicinal Chemical Biology, College of Life Sciences, College of Pharmacy, Tianjin Key Laboratory of Protein Sciences, Nankai University, Tianjin 300071, China. ; Si Zhang:1.State Key Laboratory of Medicinal Chemical Biology,
期刊：Targeting FSTL1 for Multiple Fibrotic and Systemic Autoimmune Diseases
DOI：10.1016/j.ymthe.2020.09.031
影响因子 ：12.4
引用产品： 人滑膜细胞
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Preventive effect of dioscin against monosodium urate-mediated gouty arthritis through inhibiting inflammasome NLRP3 and TLR4/NF-κB signaling pathway activation: an in vivo and in vitro study (2008-00-20)
作者：Jieru Han:1.Department of Synopsis of the Golden Chamber, School of Basic Medical Sciences, Heilongjiang University of Chinese Medicine, 24 Heping Road, Harbin, 150040, Heilongjiang, People's Republic of China. ; Guangyu Shi:1.Department of Ultrasound Medicine, First Affiliated Hospital, Heilongjiang University of Chinese Medicine, Harbin, 150040, Heilongjiang, People's Republic of China. ; Wenhao Li:1.Department of Synopsis of the Golden Chamber, School of Basic Medical Sciences, Heilongjiang University of Chinese Medicine, 24 Heping Road, Harbin, 150040, Heilongjiang, People's Republic of China. ; Ying Xie:1.Department of Synopsis of the Golden Chamber, School of Basic Medical Sciences, Heilongjiang University of Chinese Medicine, 24 Heping Road, Harbin, 150040, Heilongjiang, People's Republic of China. ; Fuzhen Li:1.Department of Synopsis of the Golden Chamber, School of Basic Medical Sciences, Heilongjiang University of Chinese Medicine, 24 Heping Road, Harbin, 150040, Heilongj
期刊：Preventive effect of dioscin against monosodium urate-mediated gouty arthritis through inhibiting inflammasome NLRP3 and TLR4/NF-κB signaling pathway activation: an in vivo and in vitro study
DOI：10.1007/s11418-020-01440-7
影响因子 ：3.3
引用产品： 人滑膜细胞
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