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人滑膜成纤维细胞(类风湿关节炎)
Human Synovial Fibroblast Cells (rheumatoid arthritis)
货号：CP-H248
规格：5×10⁵Cells/T25培养瓶
价格： ￥7380
培养基货号： CM-H248
产品概述
人滑膜成纤维细胞(类风湿关节炎)
Cat NO.: CP-H248
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产品名称：人滑膜成纤维细胞(类风湿关节炎)
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组织来源：滑膜组织
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产品规格：5×10⁵Cells/T25培养瓶
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细胞简介：
人滑膜成纤维细胞(类风湿关节炎)分离自滑膜组织；滑膜组织是位于关节腔内面的内衬结构，各种关节内疾病均会累及滑膜。而滑膜细胞是维持关节正常功能的重要组织结构，同时在各种关节疾患中也是主要病变部位。骨关节炎(OA)以关节软骨退行性变为特征，其病理改变累及关节的各个组成部分，但绝不仅局限于软骨，还包括软骨下骨、滑膜、半月板和韧带。各组成部分的病理改变相互影响，相互作用，共同加速关节的退变。滑膜细胞是构成滑膜层的最大细胞群体，是维持关节正常功能的重要组织结构，它包埋在颗粒状无定性的基质中，基质内有分散的纤维分布。滑膜由A型(巨噬样滑膜细胞)、B型(成纤维样滑膜细胞)以及C型(树突细胞样滑膜细胞)细胞组成。类风湿关节炎(RA)是一种病因未明的慢性、以炎性滑膜炎为主的系统性疾病。其特征是手、足小关节的多关节、对称性、侵袭性关节炎症，经常伴有关节外器官受累及血清类风湿因子阳性，可以导致关节畸形及功能丧失。RA的发病可能与遗传、感染、性激素等有关。RA关节炎的病理主要有滑膜衬里细胞增生、间质大量炎性细胞浸润，以及微血管的新生、血管翳的形成及软骨和骨组织的破坏等。体外培养人滑膜成纤维细胞(类风湿关节炎)对于研究类风湿关节炎疾病的发生机理、治疗具有重要意义。
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方法简介：
普诺赛实验室分离的人滑膜成纤维细胞(类风湿关节炎)采用混合酶消化结合差速贴壁法制备而来，细胞总量约为5×10⁵cells/瓶。
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质量检测：
普诺赛实验室分离的人滑膜成纤维细胞(类风湿关节炎)经Vimentin免疫荧光鉴定，纯度可达90%以上，且不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等。
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培养信息：
包被条件 培养基 含FBS、生长添加剂、Penicillin、Streptomycin等 产品货号 CM-H248 换液频率 每2-3天换液一次 生长特性 贴壁 细胞形态 成纤维细胞样 传代特性 可传3-5代左右 消化液 0.25%胰蛋白酶 人滑膜成纤维细胞(类风湿关节炎)体外培养周期有限；建议使用普诺赛配套的专用生长培养基及正确的操作方法来培养，以此保证该细胞的最佳培养状态。
参考文献
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USP2 Promotes the Proliferation and Inflammation of Fibroblast-Like Synovial Cells in Rheumatoid Arthritis Through Deubiquitination of TRAF2 (2024-03-13)
作者：Xiuchan Liu:1.Department of Rheumatology and Immunology, Tianjin Medical University General Hospital, Tianjin, China. ; 2.Department of Infectious Diseases, Tianjin University Tianjin Hospital, Tianjin, China. ; Geng Zhang:1.Department of Infectious Diseases, Tianjin Medical University Baodi Clinical College, Tianjin, China. ; Lei Liu:1.Department of Infectious Diseases, Tianjin Medical University Baodi Clinical College, Tianjin, China. ; Guangyi Xiong:1.Department of Pathology, Tianjin University Tianjin Hospital, Tianjin, China. ; Jun Liu:1.Department of Joint Surgery, Tianjin University Tianjin Hospital, Tianjin, China. tjyyliujun@163.com. ; Wei Wei:1.Department of Rheumatology and Immunology, Tianjin Medical University General Hospital, Tianjin, China. tjweiwei2003@163.com. ;
期刊：USP2 Promotes the Proliferation and Inflammation of Fibroblast-Like Synovial Cells in Rheumatoid Arthritis Through Deubiquitination of TRAF2
DOI：10.1007/s10528-024-10737-1
影响因子 ：2.4
引用产品： 人滑膜成纤维细胞 (类风湿关节炎)
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Verification of cuproptosis-related diagnostic model associated with immune infiltration in rheumatoid arthritis (2023-07-20)
作者：Mingyang Jiang:1.Department of Bone and Joint Surgery, Guangxi Medical University First Affiliated Hospital, Nanning, China. ; Kaicheng Liu:1.Department of Bone and Joint Surgery, Guangxi Medical University First Affiliated Hospital, Nanning, China. ; Shenyi Lu:1.Department of Rehabilitation, The Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, China. ; Yue Qiu:1.Department of Bone and Joint Surgery, Guangxi Medical University First Affiliated Hospital, Nanning, China. ; Xiaochong Zou:1.Department of Bone and Joint Surgery, Guangxi Medical University First Affiliated Hospital, Nanning, China. ; Ke Zhang:1.Department of Bone and Joint Surgery, Guangxi Medical University First Affiliated Hospital, Nanning, China. ; Chuanliang Chen:1.Department of Bone and Joint Surgery, Guangxi Medical University First Affiliated Hospital, Nanning, China. ; Yiji Jike:1.Department of Bone and Joint Surgery, Guangxi Medical University First Affiliated Hospital, Nanning, C
期刊：Verification of cuproptosis-related diagnostic model associated with immune infiltration in rheumatoid arthritis
DOI：10.3389/fendo.2023.1204926
影响因子 ：5.2
引用产品： 人滑膜成纤维细胞 (类风湿关节炎) , 人滑膜细胞
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FNDC4 reduces inflammation, proliferation, invasion and migration of rheumatoid synovial cells by inhibiting CCL2/ERK signaling (2023-12-23)
作者：Changjuan Xiao:1.Nephrology Department, Hunan Provincial People's Hospital (The First Affiliated Hospital of Hunan Normal University), Changsha 410000, Hunan, P.R. China. Electronic address: Xiaochange870425@163.com. ; Shuoshan Xie:1.Nephrology Department, Hunan Provincial People's Hospital (The First Affiliated Hospital of Hunan Normal University), Changsha 410000, Hunan, P.R. China. ; Shaxi Ouyang:1.Nephrology Department, Hunan Provincial People's Hospital (The First Affiliated Hospital of Hunan Normal University), Changsha 410000, Hunan, P.R. China. ;
期刊：FNDC4 reduces inflammation, proliferation, invasion and migration of rheumatoid synovial cells by inhibiting CCL2/ERK signaling
DOI：10.1016/j.tice.2023.102294
影响因子 ：2.6
引用产品： 人滑膜成纤维细胞 (类风湿关节炎) , 人滑膜成纤维细胞
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Circ_0000479 promotes proliferation, invasion, migration and inflammation and inhibits apoptosis of rheumatoid arthritis fibroblast-like synoviocytes via miR-766/FKBP5 axis (2023-03-21)
作者：Peng Zhao:1.Department of Rheumatology Immunology, 3201 Hospital, 783 Tianhan Avenue, Hantai District, Hanzhong, 723000, China. pengzhao3201@163.com. ; Guobin Ma:1.Department of Rheumatology Immunology, 3201 Hospital, 783 Tianhan Avenue, Hantai District, Hanzhong, 723000, China. ; Lintong Ma:1.Department of Hematology, 3201 Hospital, Hanzhong, China. ;
期刊：Circ_0000479 promotes proliferation, invasion, migration and inflammation and inhibits apoptosis of rheumatoid arthritis fibroblast-like synoviocytes via miR-766/FKBP5 axis
DOI：10.1186/s13018-023-03700-0
影响因子 ：2.6
引用产品： 人滑膜成纤维细胞 (类风湿关节炎) , 人滑膜成纤维细胞 (类风湿关节炎)
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Silence of S1PR4 Represses the Activation of Fibroblast-like Synoviocytes by Regulating IL-17/STAT3 Signaling Pathway (2022-09-06)
作者：Pengyu Zhang:1.Department of Nephropathy and Rheumatism, Tongde Hospital of Zhejiang Province, Hangzhou, 310007, Zhejiang, China. ; Qiang Zhang:1.Department of Rheumatology and Immunology, The 962Nd Hospital of The PLA Joint Logistic Support Force, Harbin, Heilongjiang, 150080, China. ; Zhenxia Shao:1.Department of Gynaecology, Shaoxing Second Hospital, No. 123 Yan'an Road, Shaoxing, 312000, Zhejiang, China. shaozhenxia01@163.com. ;
期刊：Silence of S1PR4 Represses the Activation of Fibroblast-like Synoviocytes by Regulating IL-17/STAT3 Signaling Pathway
DOI：10.1007/s10753-022-01728-8
影响因子 ：4.7
引用产品： 人滑膜成纤维细胞 , 人滑膜成纤维细胞 (类风湿关节炎)
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