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产品概述
人软骨细胞
Cat NO.: CP-H107
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产品名称:人软骨细胞
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组织来源:软骨组织
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产品规格:5×10⁵Cells/T25培养瓶
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细胞简介:
人软骨细胞分离自软骨组织;软骨组织由软骨细胞、基质及纤维构成。软骨组织再生能力强,这些增生的幼稚细胞形似纤维母细胞,以后逐渐变为软骨母细胞,并形成软骨基质,细胞被埋在软骨陷窝内而变为静止的软骨细胞。根据软骨组织内所含纤维成分的不同,可将软骨分为透明软骨、弹性软骨和纤维软骨三种,其中以透明软骨的分布较广,结构也较典型。软骨细胞(chondrocyte)位于软骨陷窝内。幼稚的软骨细胞位于软骨组织的表层,单个分布,体积较小,呈椭圆形,长轴与软骨表面平行,越向深层的软骨细胞体积之间增大呈圆形,细胞核圆形或卵圆形,染色浅,细胞质弱嗜碱性,常见数量不一的脂滴。成熟的软骨细胞多2-8个成群分布于软骨陷窝内,这些软骨细胞由同一个母细胞分裂增殖而成,称为同源细胞群。电镜下,软骨细胞有突起和皱褶,细胞质内有大量的粗面内质网和发达的高尔基复合体及少量的线粒体。在组织切片中,软骨细胞收缩为不规则形,在软骨囊和细胞之间出现较大的腔隙。体外培养的软骨细胞对于研究其生理功能、药物作用以及各种致病因素作用下的病理生理改变具重要意义。
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方法简介:
普诺赛实验室分离的人软骨细胞采用胶原酶 - 中性蛋白酶联合消化制备而来,细胞总量约为5×10⁵cells/瓶。
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质量检测:
普诺赛实验室分离的人软骨细胞经Ⅱ型胶原蛋白免疫荧光鉴定,纯度可达90%以上,且不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等。
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培养信息:
包被条件 培养基 含FBS、生长添加剂、Penicillin、Streptomycin等 产品货号 CM-H107 换液频率 每2-3天换液一次 生长特性 贴壁 细胞形态 梭形、多角形 传代特性 可传5代左右;3代以内状态最佳 消化液 0.25%胰蛋白酶 人软骨细胞体外培养周期有限;建议使用普诺赛配套的专用生长培养基及正确的操作方法来培养,以此保证该细胞的最佳培养状态。
参考文献
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Arctiin alleviates the progression of osteoarthritis by regulating the cholesterol metabolic pathway (2025-01-02)
期刊:Scientific Reports
DOI:10.1038/s41598-024-83993-1
影响因子 :3.8
引用产品: 人软骨细胞
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Investigating Novel Therapeutic Approaches for Idiopathic Short Stature: Targeting siRNA and Growth Hormone Delivery to the Growth Plate Using Exosome Nanoparticles (2024-04-19)
期刊:Advanced Science
影响因子 :14.3
引用产品: 人软骨细胞
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HWJMSC-EVs promote cartilage regeneration and repair via the ITGB1/TGF-β/Smad2/3 axis mediated by microfractures (2024-04-12)
期刊:JOURNAL OF NANOBIOTECHNOLOGY
DOI:10.1186/s12951-024-02451-2
影响因子 :10.2
引用产品: 人软骨细胞 , 人骨髓间充质干细胞成软骨诱导分化培养基 , 人骨髓间充质干细胞
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High paracrine activity of hADSCs cartilage microtissues inhibits extracellular matrix degradation and promotes cartilage regeneration (2024-11-29)
期刊:Materials Today Bio
DOI:10.1016/j.mtbio.2024.101372
影响因子 :8.7
引用产品: 人软骨细胞
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Garlic-derived Exosomes Alleviate Osteoarthritis Through Inhibiting the MAPK Signaling Pathway (2024-08-27)
期刊:APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
DOI:10.1007/s12010-024-05047-6
影响因子 :3.1
引用产品: 人软骨细胞
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S100A12 is involved in the pathology of osteoarthritis by promoting M1 macrophage polarization via the NF-κB pathway (2024-03-16)
期刊:CONNECTIVE TISSUE RESEARCH
DOI:10.1080/03008207.2024.2310852
影响因子 :2.9
引用产品: 人软骨细胞
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SRGN promotes macrophage recruitment through CCL3 in osteoarthritis (2024-07-27)
期刊:CONNECTIVE TISSUE RESEARCH
DOI:10.1080/03008207.2024.2380313
影响因子 :2.8
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A Novel Dinuclear Complex-PLC Composite Material as Fluorescent Switch in Treatment on Osteoarthritis (2024-06-11)
期刊:JOURNAL OF FLUORESCENCE
DOI:10.1007/s10895-024-03787-6
影响因子 :2.6
引用产品: 人软骨细胞
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Long Non-coding RNA DLEU1 Promotes Progression of Osteoarthritis via miR-492/TLR8 Axis (2024-12-01)
期刊:CURRENT PHARMACEUTICAL BIOTECHNOLOGY
DOI:10.2174/0113892010275579240116061104
影响因子 :2.2
引用产品: 人软骨细胞
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Knocking-down long non-coding RNA LINC01094 prohibits chondrocyte apoptosis via regulating microRNA-577/metal-regulatory transcription factor 1 axis (2024-05-17)
期刊:Journal of Orthopaedic Surgery
影响因子 :1.6
引用产品: 人软骨细胞
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Andrographolide suppresses fibrogenic phenotype of chondrocytes and ameliorates osteoarthritis by regulating miR-137/BMP7 axis (2024-11-26)
期刊:Journal of Orthopaedics
影响因子 :1.5
引用产品: 人软骨细胞完全培养基 , 人软骨细胞
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Lubricating Micro-Interface Assisted General Strategy for Preparing dECM-Microparticle–Based Heterogeneous Granular Inks toward 3D Printing (2023-12-11)
期刊:ADVANCED FUNCTIONAL MATERIALS
影响因子 :19.0
引用产品: 人软骨细胞
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Plasma exosome miRNA-26b-3p derived from idiopathic short stature impairs longitudinal bone growth via the AKAP2/ERK1/2 axis (2023-03-16)
期刊:JOURNAL OF NANOBIOTECHNOLOGY
DOI:10.1186/s12951-023-01849-8
影响因子 :10.2
引用产品: 人软骨细胞
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Inhibition of miR-182-5p Targets FGF9 to Alleviate Osteoarthritis (2023-03-29)
期刊:Analytical Cellular Pathology
影响因子 :3.2
引用产品: 人软骨细胞
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Prognostic-related genes for pancreatic cancer typing and immunotherapy response prediction based on single-cell sequencing data and bulk sequencing data (2023-07-21)
期刊:ONCOLOGY RESEARCH
影响因子 :3.1
引用产品: 人软骨细胞
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Acrylamide induces human chondrocyte cell death by initiating autophagy?dependent ferroptosis (2023-04-12)
期刊:Experimental and Therapeutic Medicine
影响因子 :2.7
引用产品: 人软骨细胞 , 人软骨细胞完全培养基
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Ferroptosis-related genes LPCAT3 and PGD are potential diagnostic biomarkers for osteoarthritis (2023-09-19)
期刊:Journal of Orthopaedic Surgery and Research
DOI:10.1186/s13018-023-04128-2
影响因子 :2.6
引用产品: 人软骨细胞
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Downregulation of lncRNA NEAT1 interacts with miR-374b-5p/PGAP1 axis to aggravate the development of osteoarthritis (2023-09-11)
期刊:Journal of Orthopaedic Surgery and Research
DOI:10.1186/s13018-023-04147-z
影响因子 :2.6
引用产品: 人软骨细胞
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LCN2 is a new diagnostic biomarker and potential therapeutic target in idiopathic short stature (2022-05-24)
期刊:JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
影响因子 :5.3
引用产品: 人软骨细胞
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m6A Regulator-Mediated RNA Methylation Modification Patterns Regulate the Immune Microenvironment in Osteoarthritis (2022-06-23)
期刊:Frontiers in Genetics
影响因子 :4.8
引用产品: 人软骨细胞
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Downregulation of miR-3680–3p inhibits the progression of osteoarthritis via targeting OGG1 (2022-01-21)
期刊:Archives of Gerontology and Geriatrics
DOI:10.1016/j.archger.2022.104626
影响因子 :4.0
引用产品: 人软骨细胞
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Regulation of circADAMTS6-miR-324-5p-PIK3R3 ceRNA pathway may be a novel mechanism of IL-1β-induced osteoarthritic chondrocytes (2022-03-25)
期刊:Journal Of Bone And Mineral Metabolism
DOI:10.1007/s00774-021-01308-0
影响因子 :3.3
引用产品: 人软骨细胞 , 293T [HEK-293T] 细胞
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Circ_0136474 promotes the progression of osteoarthritis by sponging mir-140-3p and upregulating MECP2 (2022-11-26)
期刊:JOURNAL OF MOLECULAR HISTOLOGY
DOI:10.1007/s10735-022-10100-x
影响因子 :3.2
引用产品: 人软骨细胞
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Circ_0043947 contributes to interleukin 1β-induced injury in chondrocytes by sponging miR-671-5p to up-regulate RTN3 expression in osteoarthritis pathology (2022-03-24)
期刊:Journal of Orthopaedic Surgery and Research
DOI:10.1186/s13018-022-02970-4
影响因子 :2.6
引用产品: 人软骨细胞 , 人软骨细胞完全培养基
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Circ_SPG11 plays contributing effects on IL-1β-induced chondrocyte apoptosis and ECM degradation via miR-665 inhibition-mediated GREM1 upregulation (2021-11-17)
期刊:Clinical Immunology
DOI:10.1016/j.clim.2021.108889
影响因子 :8.6
引用产品: 人软骨细胞
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Activation of microglial GLP-1R in the trigeminal nucleus caudalis suppresses central sensitization of chronic migraine after recurrent nitroglycerin stimulation (2021-07-29)
期刊:JOURNAL OF HEADACHE AND PAIN
DOI:10.1186/s10194-021-01302-x
影响因子 :7.4
引用产品: 人软骨细胞
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Circular RNA circANAPC2 mediates the impairment of endochondral ossification by miR‐874‐3p/SMAD3 signalling pathway in idiopathic short stature (2021-03-13)
期刊:Journal Of Cellular And Molecular Medicine
影响因子 :5.3
引用产品: 人软骨细胞
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A Novel Diagnostic Predictive Model for Idiopathic Short Stature in Children (2021-09-07)
期刊:Frontiers in Endocrinology
影响因子 :5.2
引用产品: 人软骨细胞
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Hsa_circ_0134111 promotes osteoarthritis progression by regulating miR-224-5p/CCL1 interaction (2021-08-19)
期刊:Aging-US
影响因子 :5.2
引用产品: 人软骨细胞
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Valproic Acid Protects Chondrocytes from LPS-Stimulated Damage via Regulating miR-302d-3p/ITGB4 Axis and Mediating the PI3K-AKT Signaling Pathway (2021-04-22)
期刊:Frontiers in Molecular Biosciences
影响因子 :5.0
引用产品: 人软骨细胞
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miR-590-5p affects chondrocyte proliferation, apoptosis, and inflammation by targeting FGF18 in osteoarthritis (2021-08-15)
期刊:American Journal of Translational Research
DOI:PMID:34539990
影响因子 :3.9
引用产品: 人软骨细胞
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Downregulation of miR-1-3p expression inhibits the hypertrophy and mineralization of chondrocytes in DDH (2021-08-18)
期刊:Journal of Orthopaedic Surgery and Research
DOI:10.1186/s13018-021-02666-1
影响因子 :2.6
引用产品: 人软骨细胞
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Fabrication of chondroitin sulfate calcium complex and its chondrocyte proliferation in vitro (2020-10-22)
期刊:Carbohydrate Polymers
DOI:10.1016/j.carbpol.2020.117282
影响因子 :11.2
引用产品: 人软骨细胞
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Hsa_circ_0005567 Activates Autophagy and Suppresses IL-1β-Induced Chondrocyte Apoptosis by Regulating miR-495 (2020-08-25)
期刊:Frontiers in Molecular Biosciences
影响因子 :5.0
引用产品: 人软骨细胞
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Hsa_circularRNA_0079201 suppresses chondrocyte proliferation and endochondral ossification by regulating the microRNA‑140‑3p/SMAD2 signaling pathway in idiopathic short stature (2020-09-25)
期刊:INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
影响因子 :3.1
引用产品: 人软骨细胞
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Chondrocyte suppression is mediated by miR‐129‐5p via GDF11/SMAD3 signaling in developmental dysplasia of the hip (2020-05-12)
期刊:Journal of Orthopaedic Surgery and Research
影响因子 :2.6
引用产品: 人软骨细胞
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Silver nanoparticles inhibit the function of hypoxia-inducible factor-1 and target genes: insight into the cytotoxicity and antiangiogenesis (2016-12-08)
期刊:International Journal of Nanomedicine
影响因子 :8.0
引用产品: 人软骨细胞
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