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人表皮角质形成细胞
Human Epidermal Keratinocyte Cells
货号：CP-H113
规格：5×10⁵Cells/T25培养瓶
价格： ￥7880
培养基货号： CM-H113
产品概述
人表皮角质形成细胞
Cat NO.: CP-H113
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产品名称：人表皮角质形成细胞
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组织来源：皮肤组织
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产品规格：5×10⁵Cells/T25培养瓶
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细胞简介：
人表皮角质形成细胞分离自皮肤组织；表皮位于动物皮肤的外层，由胚胎时期外胚层形成，具有抗摩擦和抗损伤的作用。表皮是皮肤的浅层结构，由复层扁平上皮构成。从基底层到表面可分为五层，即基底层、棘层、颗粒层、透明层和角质层。表皮角质形成细胞是一种能合成角质蛋白的上皮细胞，此类细胞为表皮的主体，由表皮深层始逐渐增殖、分化，并在成为角化的角质细胞中，细胞核与细胞器完全消失，细胞亦失去生理功能而脱落。未脱落部分对机体尚有保护作用，故不必在洗擦身体时用力搓擦，使其过早脱失。表皮角质形成细胞主要分布于表皮基底层，在上皮程序性死亡后，细胞产生角化并移向表皮。体外培养的表皮角化细胞容易导致终末分化，不能充分增殖。角质形成细胞最终产生角质蛋白，在其向角质细胞演变过程中，一般可以分为四层，即基底层、棘层、颗粒层以及角质层。有人把前三层或前二层称为生发层或马尔匹基层。此外，在某些部位，特别在掌跖部位，角质层下方还可见到透明层。
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方法简介：
普诺赛实验室分离的人表皮角质形成层细胞先中性蛋白酶消化、后胰蛋白酶 - 胶原酶混合消化法制备而来，细胞总量约为5×10⁵cells/瓶。
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质量检测：
普诺赛实验室分离的人表皮角质形成细胞经PCK免疫荧光鉴定，纯度可达90%以上，且不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等。
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培养信息：
包被条件 鼠尾胶原Ⅰ(2-5μg/cm2) 培养基 含FBS、生长添加剂、Penicillin、Streptomycin等 产品货号 CM-H113 换液频率 每2-3天换液一次 生长特性 贴壁 细胞形态 上皮细胞样 传代特性 可传2-3代 消化液 0.25%胰蛋白酶 人表皮角质形成细胞体外培养周期有限；建议使用普诺赛配套的专用生长培养基及正确的操作方法来培养，以此保证该细胞的最佳培养状态。
参考文献
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Accelerating Wound Closure with Metrnl in Normal and Diabetic Mouse Skin (2023-09-08)
作者：Lingyu Song:1.Department of Pathophysiology, Guizhou Medical University, Guiyang, Guizhou, China. ; 2.Department of Gastroenterology, Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou, China. ; Xuebing Chang:1.Department of Pathophysiology, Guizhou Medical University, Guiyang, Guizhou, China. ; Laying Hu:1.Department of Pathophysiology, Guizhou Medical University, Guiyang, Guizhou, China. ; Lu Liu:1.Department of Pathophysiology, Guizhou Medical University, Guiyang, Guizhou, China. ; Guifang Wang:1.Department of Pathophysiology, Guizhou Medical University, Guiyang, Guizhou, China. ; Yali Huang:1.Department of Pathophysiology, Guizhou Medical University, Guiyang, Guizhou, China. ; Lifen Xu:1.Department of Pathology, Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou, China. ; Bangming Jin:1.Guizhou Province Talent Base of Research on the Pathogenesis and Drug Prevention and Treatment for Common Major Diseases, Guizhou Medical University, Guiyang
期刊：Accelerating Wound Closure with Metrnl in Normal and Diabetic Mouse Skin
影响因子 ：7.7
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The miR-155/GATA3/IL37 axis modulates the production of proinflammatory cytokines upon TNF‐α stimulation to affect psoriasis development (2007-00-20)
作者：Haizhen Wang:1.Department of Dermatology, The Second Affiliated Hospital, Hunan University of Chinese Medicine, Changsha, China. ; Yujin Zhang:1.Department of Dermatology, The Second Affiliated Hospital, Hunan University of Chinese Medicine, Changsha, China. ; Junzi Luomei:1.Department of Dermatology, The Second Affiliated Hospital, Hunan University of Chinese Medicine, Changsha, China. ; Pan Huang:1.Department of Dermatology, The Second Affiliated Hospital, Hunan University of Chinese Medicine, Changsha, China. ; Rong Zhou:1.Department of Dermatology, The Second Affiliated Hospital, Hunan University of Chinese Medicine, Changsha, China. ; Youhua Peng:1.Department of Dermatology, The Second Affiliated Hospital, Hunan University of Chinese Medicine, Changsha, China. ;
期刊：The miR-155/GATA3/IL37 axis modulates the production of proinflammatory cytokines upon TNF‐α stimulation to affect psoriasis development
影响因子 ：3.6
引用产品： Hacat 细胞 , MEM (含NEAA) 培养基 , 特级胎牛血清 , 青霉素-链霉素溶液 (双抗)，100× , 人表皮角质形成细胞 , 人表皮角质形成细胞完全培养基
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Magnetic nanoparticles with low Curie temperature and high heating efficiency for self-regulating temperature hyperthermia (2005-09-20)
作者：Wei Zhang: 1.State Key Laboratory of Structure Analysis for Industrial Equipment, Department of Engineering Mechanics, Dalian University of Technology, Dalian 116024, China; Xiaogang Yu: 1.State Key Laboratory of Structure Analysis for Industrial Equipment, Department of Engineering Mechanics, Dalian University of Technology, Dalian 116024, China; Heng Li: 1.State Key Laboratory of Structure Analysis for Industrial Equipment, Department of Engineering Mechanics, Dalian University of Technology, Dalian 116024, China; Dili Dong: 1.State Key Laboratory of Structure Analysis for Industrial Equipment, Department of Engineering Mechanics, Dalian University of Technology, Dalian 116024, China; Xudong Zuo: 1.State Key Laboratory of Structure Analysis for Industrial Equipment, Department of Engineering Mechanics, Dalian University of Technology, Dalian 116024, China; Cheng-wei Wu: 1.State Key Laboratory of Structure Analysis for Industrial Equipment, Department of Engineering Mechanics, Dalian
期刊：Magnetic nanoparticles with low Curie temperature and high heating efficiency for self-regulating temperature hyperthermia
DOI：10.1016/j.jmmm.2019.165382
影响因子 ：2.7
引用产品： Hacat 细胞 , 人表皮角质形成细胞
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