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产品概述
人真皮成纤维细胞
Cat NO.: CP-H103
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产品名称：人真皮成纤维细胞
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组织来源：皮肤组织
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产品规格：5×10⁵Cells/T25培养瓶
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细胞简介：
人真皮成纤维细胞分离自皮肤组织内的真皮层；皮肤组织来源包括头部、脸部、手、脚、腿、背部、腹部、包皮等体表部位皮肤，可依据需求选用；真皮，位于表皮深层，向下与皮下组织相连，真皮结缔组织的胶原纤维和弹性纤维互相交织在一起，埋于基质内。其内分布着各种结缔组织细胞和大量的胶原纤维弹性纤维，使皮肤既有弹性，又有韧性。其由两层组成——乳头层与网状层。真皮的结构组成是胶原蛋白、弹性纤维以及基质。成纤维细胞(Fibroblast)是疏松结缔组织的主要细胞成分，由胚胎时期的间充质细胞分化而来。成纤维细胞较大，轮廓清楚，多为突起的纺锤形或星形的扁平状结构，其细胞核呈规则的卵圆形，核仁大而明显。成纤维细胞功能活动旺盛，细胞质嗜弱碱性，具明显的蛋白质合成和分泌活动，在一定条件下，它可以实现跟纤维细胞的互相转化；成纤维细胞对不同程度的细胞变性、坏死和组织缺损的修复有着十分重要的作用。刚分离的真皮成纤维细胞呈圆形、折光性良好，悬浮于培养基中。30min细胞贴壁，其中部分开始伸出伪足，表现为小的突起；6h后细胞基本贴壁完全，伸展成梭形，胞核清晰，分布较均匀，散在生长，不聚集成团；细胞生长迅速，5-7天即呈融合状态，细胞排列紧密，有的交叉重叠生长，平坦、胞体较大，细胞质透明，细胞核较大，呈椭圆形，颜色淡。细胞融合，并彼此连接成网状；细胞呈突起的纺锤形或星形的扁平分布。真皮成纤维细胞在生理条件下的主要功能包括：构造和维持组织的正常形态，合成和释放细胞外基质以及组织损伤后及时大量聚集修复损伤组织。
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方法简介：
普诺赛实验室分离的人真皮成纤维细胞采用先中性蛋白酶消化、后胰蛋白酶 - 胶原酶混合消化法结合差速贴壁法制备而来，细胞总量约为5×10⁵cells/瓶。
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质量检测：
普诺赛实验室分离的人真皮成纤维细胞经Vimentin免疫荧光鉴定，纯度可达90%以上，且不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等。
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培养信息：
包被条件 培养基 含FBS、生长添加剂、Penicillin、Streptomycin等 产品货号 CM-H103 换液频率 每2-3天换液一次 生长特性 贴壁 细胞形态 成纤维细胞样 传代特性 可传5代左右；3代以内状态最佳 消化液 0.25%胰蛋白酶 人真皮成纤维细胞体外培养周期有限；建议使用普诺赛配套的专用生长培养基及正确的操作方法来培养，以此保证该细胞的最佳培养状态。
参考文献
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Nanosphere-reinforced polysaccharide self-healing hydrogels for infected wound healing (2024-03-02)
作者：Yikun Ju:1.Department of Plastic and Aesthetic (Burn) Surgery, The Second Xiangya Hospital, Central South University, Changsha 410011, China. ; Huaqian Xue:1.School of Pharmacy, Ningxia Medical University, Ningxia 750004, China. ; Pu Yang:1.Department of Plastic and Aesthetic (Burn) Surgery, The Second Xiangya Hospital, Central South University, Changsha 410011, China. ; Xiangjun Liu:1.Department of Plastic and Aesthetic (Burn) Surgery, The Second Xiangya Hospital, Central South University, Changsha 410011, China. ; Jingjing Sun:1.Hunan University, Changsha 410082, China. ; Mengyi Yu:1.Hunan University, Changsha 410082, China. ; Lanjie Lei:1.Institute of Translational Medicine, Zhejiang Shuren University, Hangzhou 310015, China. Electronic address: leilanjie1988@163.com. ; Bairong Fang:1.Department of Plastic and Aesthetic (Burn) Surgery, The Second Xiangya Hospital, Central South University, Changsha 410011, China. Electronic address: fbrfbr2004@csu.edu.cn. ;
期刊：Nanosphere-reinforced polysaccharide self-healing hydrogels for infected wound healing
DOI：10.1016/j.ijbiomac.2024.130593
影响因子 ：8.2
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Folate-Modified pH and ROS Dual-Responsive Polymeric Nanocarriers for Targeted Anticancer Drug Delivery (2024-03-28)
作者：Fanjia Dai: 1.School of Materials Science and Chemical Engineering, Ningbo University, Ningbo 315211, China; Fengjiao Chen: 1.School of Materials Science and Chemical Engineering, Ningbo University, Ningbo 315211, China; Jiaying Zhang: 1.School of Materials Science and Chemical Engineering, Ningbo University, Ningbo 315211, China; Xianwu Chen: 1.The Affiliated Hospital of Medical School, Ningbo University, Ningbo 315211, China; Hongze Liang: 1.School of Materials Science and Chemical Engineering, Ningbo University, Ningbo 315211, China; Zhenjiang Liang: 1.Center for Child Care and Mental Health, Shenzhen Children’s Hospital Affiliated to Shantou University Medical College, Shenzhen 518026, China; Shun Zhang: 1.Key Laboratory of Diagnosis and Treatment of Digestive System Tumors of Zhejiang Province, Ningbo Huamei Hospital, University of Chinese Academy of Sciences, Ningbo 315000, China; Hui Tan: 1.Center for Child Care and Mental Health, Shenzhen Children’s Hospital Affiliated to Shant
期刊：Folate-Modified pH and ROS Dual-Responsive Polymeric Nanocarriers for Targeted Anticancer Drug Delivery
影响因子 ：5.9
引用产品： Hep G2 细胞 , 人真皮成纤维细胞 , MEM (含NEAA) 培养基 , 青霉素-链霉素溶液 (双抗)，100×
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Sustained release of magnesium and zinc ions synergistically accelerates wound healing (2023-02-24)
作者：Fan Yang:1.Department of Prosthodontics, Peking University Hospital of Stomatology, Beijing, 100081, China. ; 2.National Center for Stomatology, National Clinical Research Center for Oral Diseases, National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, Beijing Key Laboratory of Digital Stomatology, Research Center of Engineering and Technology for Computerized Dentistry Ministry of Health, NMPA Key Laboratory for Dental Materials, Beijing, 100081, China. ; Yijia Xue:1.Department of Prosthodontics, Peking University Hospital of Stomatology, Beijing, 100081, China. ; 2.National Center for Stomatology, National Clinical Research Center for Oral Diseases, National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, Beijing Key Laboratory of Digital Stomatology, Research Center of Engineering and Technology for Computerized Dentistry Ministry of Health, NMPA Key Laboratory for Dental Materials, Beijing, 100081, China. ; Feilong Wan
期刊：Sustained release of magnesium and zinc ions synergistically accelerates wound healing
DOI：10.1016/j.bioactmat.2023.02.019
影响因子 ：18.9
引用产品： 人真皮成纤维细胞 , Hacat 细胞
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Placental mesenchymal stem cells-secreted proenkephalin suppresses the p38 MAPK signaling to block hyperproliferation of keloid fibroblasts (2023-09-11)
作者：Di Wu:1.Department of Dermtology, Jilin Central Hospital, Jilin 132001, Jilin, PR China; Department of Dermtology, Affiliated Hospital of Yanbian University, Yanji 133000, Jilin, PR China. ; Xiao Liu:1.Department of Dermtology, Jilin Central Hospital, Jilin 132001, Jilin, PR China. ; Zhehu Jin:1.Department of Dermtology, Affiliated Hospital of Yanbian University, Yanji 133000, Jilin, PR China. Electronic address: Jinzh_47@163.com. ;
期刊：Placental mesenchymal stem cells-secreted proenkephalin suppresses the p38 MAPK signaling to block hyperproliferation of keloid fibroblasts
DOI：10.1016/j.tice.2023.102218
影响因子 ：2.6
引用产品： 人瘢痕疙瘩成纤维细胞 , 人真皮成纤维细胞 , 人胎盘间充质干细胞 , 人瘢痕疙瘩成纤维细胞完全培养基 , 人真皮成纤维细胞完全培养基 , 人胎盘间充质干细胞完全培养基
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Photocatalytic glucose depletion and hydrogen generation for diabetic wound healing (2022-09-27)
作者：Shengqiang Chen:1.Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, School of Biomedical Engineering, Health Science Center, Shenzhen University, Shenzhen, China. ; Yanxia Zhu:1.Department of Cell Biology and Medical Genetics, School of Basic Medical Sciences, Shenzhen University Health Science Center, Shenzhen, China. ; Qingqing Xu:1.Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, School of Biomedical Engineering, Health Science Center, Shenzhen University, Shenzhen, China. ; Qi Jiang:1.Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, School of Biomedical Engineering, Health Science Center, Shenzhen University, Shenzhen, China. ; Danyang Chen:1.Shanghai Key Laboratory of Hydrogen Science & Shanghai Key Laboratory of Hydrogen Science & Center of Hydrogen Science, School of Materials Science and Engineering, Shanghai Jiao Tong University, Shanghai, China. ; Ting Chen:1.Guangdong Key Laboratory for B
期刊：Photocatalytic glucose depletion and hydrogen generation for diabetic wound healing
DOI：10.1038/s41467-022-33475-7
影响因子 ：17.7
引用产品： Hacat 细胞 , 人真皮成纤维细胞 , HMEC-1 细胞 , HMEC-1细胞专用培养基
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Two-Dimensional Mg2Si Nanosheet-Enabled Sustained Hydrogen Generation for Improved Repair and Regeneration of Deeply Burned Skin (2022-12-22)
作者：Yanxia Zhu:1.School of Biomedical Engineering, Health Science Center, Shenzhen University, Shenzhen, Guangdong, 518060, P. R. China. ; 2.Department of Cell Biology and Medical Genetics, School of Basic Medical Sciences, Shenzhen University Health Science Center, Shenzhen, 518060, P. R. China. ; Qi Jiang:1.School of Biomedical Engineering, Health Science Center, Shenzhen University, Shenzhen, Guangdong, 518060, P. R. China. ; Zhaokui Jin:1.School of Biomedical Engineering, Health Science Center, Shenzhen University, Shenzhen, Guangdong, 518060, P. R. China. ; Danyang Chen:1.School of Biomedical Engineering, Health Science Center, Shenzhen University, Shenzhen, Guangdong, 518060, P. R. China. ; 2.Shanghai Key Laboratory of Hydrogen Science and Center of Hydrogen Science, Shanghai Jiao Tong University, Shanghai, 200240, P. R. China. ; 3.Shenzhen Research Institute, Shanghai Jiao Tong University, Shenzhen, 518057, P. R. China. ; Qingqing Xu:1.School of Biomedical Engineering, Health Sc
期刊：Two-Dimensional Mg2Si Nanosheet-Enabled Sustained Hydrogen Generation for Improved Repair and Regeneration of Deeply Burned Skin
影响因子 ：11.1
引用产品： Hacat 细胞 , 人真皮成纤维细胞 , HMEC-1 细胞 , HMEC-1细胞专用培养基
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Platelet-rich plasma promotes diabetic ulcer repair through inhibition of ferroptosis (2022-10-01)
作者：Li Chen:1.Department of Immunology, School of Basic Medical Sciences, Anhui Medical University, Hefei, China. ; 2.Department of Blood Transfusion, The First Affiliated Hospital Bengbu Medical College, Bengbu, China. ; Daoai Wu:1.Department of Endocrinology, The First Affiliated Hospital Bengbu Medical College, Bengbu, China. ; Lili Zhou:1.Department of Immunology, School of Basic Medical Sciences, Anhui Medical University, Hefei, China. ; 2.Department of Hematology, The First Affiliated Hospital Bengbu Medical College, Bengbu, China. ; Yan Ye:1.Department of Immunology, School of Basic Medical Sciences, Anhui Medical University, Hefei, China. ;
期刊：Platelet-rich plasma promotes diabetic ulcer repair through inhibition of ferroptosis
影响因子 ：3.6
引用产品： 人真皮成纤维细胞 , EA.hy926 细胞
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Jade material in vitro and in vivo: A study on the anti-inflammatory and repair efficacy of jade material on the skin (2022-12-01)
作者：Xiaohong Shu:1.Center of Cosmetic Evaluation, West China Hospital, Sichuan University, Chengdu, China. ; Ling Wang:1.Sichuan Santai Pharmaceutical Technology Co. LTD, Chengdu, China. ; Ruina Qu:1.Sichuan Santai Pharmaceutical Technology Co. LTD, Chengdu, China. ; Li Li:1.Center of Cosmetic Evaluation, West China Hospital, Sichuan University, Chengdu, China. ; 2.Department of Dermatology, West China Hospital, Sichuan University, Chengdu, China. ; Xi Wang:1.Center of Cosmetic Evaluation, West China Hospital, Sichuan University, Chengdu, China. ; 2.Department of Dermatology, West China Hospital, Sichuan University, Chengdu, China. ;
期刊：Jade material in vitro and in vivo: A study on the anti-inflammatory and repair efficacy of jade material on the skin
影响因子 ：2.4
引用产品： Hacat 细胞 , 人真皮成纤维细胞 , 人真皮成纤维细胞完全培养基
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Transdermal peptide conjugated to human connective tissue growth factor with enhanced cell proliferation and hyaluronic acid synthesis activities produced by a silkworm silk gland bioreactor (2010-00-20)
作者：Yuancheng Wang:1.Biological Science Research Center, Southwest University, Chongqing, 400715, People's Republic of China. ; 2.Chongqing Key Laboratory of Sericultural Science, Southwest University, Chongqing, 400715, People's Republic of China. ; Feng Wang:1.Biological Science Research Center, Southwest University, Chongqing, 400715, People's Republic of China. ; 2.Chongqing Key Laboratory of Sericultural Science, Southwest University, Chongqing, 400715, People's Republic of China. ; 3.Chongqing Engineering and Technology Research Center for Novel Silk Materials, Southwest University, Chongqing, 400715, People's Republic of China. ; Sheng Xu:1.Biological Science Research Center, Southwest University, Chongqing, 400715, People's Republic of China. ; 2.Chongqing Key Laboratory of Sericultural Science, Southwest University, Chongqing, 400715, People's Republic of China. ; Riyuan Wang:1.Biological Science Research Center, Southwest University, Chongqing, 400715, People's Republic of Chi
期刊：Transdermal peptide conjugated to human connective tissue growth factor with enhanced cell proliferation and hyaluronic acid synthesis activities produced by a silkworm silk gland bioreactor
DOI：10.1007/s00253-020-10836-0
影响因子 ：5.0
引用产品： 人真皮成纤维细胞 , 人真皮成纤维细胞完全培养基
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5-hydroxymethylfurfural-embedded poly (vinyl alcohol)/sodium alginate hybrid hydrogels accelerate wound healing (2007-09-20)
作者：Fanhui Kong:1.School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, Guangdong 510006, China. ; Conghui Fan:1.School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, Guangdong 510006, China. ; Yulian Yang:1.School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, Guangdong 510006, China. ; Bae Hoon Lee:1.Wenzhou Institute of Biomaterials and Engineering, University of CAS, Wenzhou, Zhejiang 325011, China; School of Ophthalmology & Optometry, School of Biomedical Engineering, Wenzhou Medical University, Wenzhou, Zhejiang 325027, China. Electronic address: bhlee@wibe.ac.cn. ; Kun Wei:1.School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, Guangdong 510006, China; Wenzhou Institute of Biomaterials and Engineering, University of CAS, Wenzhou, Zhejiang 325011, China. Electronic address: weikun@scut.edu.cn. ;
期刊：5-hydroxymethylfurfural-embedded poly (vinyl alcohol)/sodium alginate hybrid hydrogels accelerate wound healing
DOI：10.1016/j.ijbiomac.2019.07.152
影响因子 ：8.2
引用产品： 人真皮成纤维细胞 , RAW 264.7 细胞 , Hacat 细胞
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