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产品概述
人微血管周细胞
Cat NO.: CP-H169
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产品名称:人微血管周细胞
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组织来源:微血管组织
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产品规格:5×10⁵Cells/T25培养瓶
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细胞简介:
人微血管周细胞分离自微血管组织;周细胞(pericyte)又称Rouget细胞和壁细胞,是一种包围全身毛细血管和静脉中内皮细胞的细胞,可以收缩。周细胞嵌入毛细血管内皮细胞的基膜中,通过物理接触和旁分泌信号与内皮细胞进行细胞通讯,监视和稳定内皮细胞的成熟过程。此外,周细胞还具有调控毛细血管血流量、细胞碎屑清除和吞噬以及血脑屏障渗透性的作用,其多功能性是目前研究的热点之一,所以对血管周细胞的生物学特征、标记物、细胞功能等的研究都为相关研究提供基础资料。周细胞产生手指状的外延以调控毛细血管的血流量。周细胞和内皮细胞之间共同拥有一个基膜,基膜上有多种细胞连接,包括多种整合素、神经钙黏素、纤连蛋白以及接合素。它还参与毛细血管直径的双向调控;毛细血管受损时,周细胞还可增殖,分化为内皮细胞和成纤维细胞。
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方法简介:
普诺赛实验室分离的人微血管周细胞采用胶原酶-中性蛋白酶联合消化法及不锈钢网筛过滤法结合密度梯度离心法制备而来,细胞总量约为5×10⁵cells/瓶。
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质量检测:
普诺赛实验室分离的人微血管周细胞经alpha-SMA免疫荧光鉴定,纯度可达90%以上,且不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等。
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培养信息:
包被条件 培养基 含FBS、生长添加剂、Penicillin、Streptomycin等 产品货号 CM-H169 换液频率 每2-3天换液一次 生长特性 贴壁 细胞形态 成纤维细胞样 传代特性 可传5代左右;3代以内状态最佳 消化液 0.25%胰蛋白酶 人微血管周细胞体外培养周期有限;建议使用普诺赛配套的专用生长培养基及正确的操作方法来培养,以此保证该细胞的最佳培养状态。
参考文献
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Hypoxia-induced P4HA1 overexpression promotes post-ischemic angiogenesis by enhancing endothelial glycolysis through downregulating FBP1 (2024-01-18)
作者:Yating Xu:1.Clinic Center of Human Gene Research, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1277 Jiefang Ave, Wuhan, 430022, China. ; 2.Department of Cardiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. ; Di Xia:1.Clinic Center of Human Gene Research, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1277 Jiefang Ave, Wuhan, 430022, China. ; 2.Hubei Key Laboratory of Metabolic Abnormalities and Vascular Aging, Huazhong University of Science and Technology, Wuhan, China. ; Kai Huang:1.Clinic Center of Human Gene Research, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1277 Jiefang Ave, Wuhan, 430022, China. huangkai1@hust.edu.cn. ; 2.Hubei Key Laboratory of Metabolic Abnormalities and Vascular Aging, Huazhong University of Science and Technology, Wuhan, China. huangkai1@hust.edu.cn. ; 3.Hubei Clinical Rese
期刊:Hypoxia-induced P4HA1 overexpression promotes post-ischemic angiogenesis by enhancing endothelial glycolysis through downregulating FBP1
DOI:10.1186/s12967-024-04872-x
影响因子 :7.4
引用产品: 人微血管周细胞 , 人微血管周细胞完全培养基
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Protocatechuic aldehyde increases pericyte coverage and mitigates pericyte damage to enhance the atherosclerotic plaque stability (2023-10-21)
作者:Lei Zhang:1.College of Traditional Chinese Medicine, Shandong University of Traditional Chinese Medicine, Jinan 250355, China. ; Yuan Li:1.Innovation Research Institute of Traditional Chinese Medicine, Shandong University of Traditional Chinese Medicine, Jinan 250355, China. ; Wenqing Yang:1.Innovation Research Institute of Traditional Chinese Medicine, Shandong University of Traditional Chinese Medicine, Jinan 250355, China. ; Lin Lin:1.Innovation Research Institute of Traditional Chinese Medicine, Shandong University of Traditional Chinese Medicine, Jinan 250355, China. ; Jie Li:1.Innovation Research Institute of Traditional Chinese Medicine, Shandong University of Traditional Chinese Medicine, Jinan 250355, China. ; Dekun Liu:1.Shool of Acupuncture-Moxibustion and Tuina, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China. ; Chao Li:1.Innovation Research Institute of Traditional Chinese Medicine, Shandong University of Traditional Chinese Medicine, Jinan
期刊:Protocatechuic aldehyde increases pericyte coverage and mitigates pericyte damage to enhance the atherosclerotic plaque stability
DOI:10.1016/j.biopha.2023.115742
影响因子 :7.5
引用产品: 人微血管周细胞
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