产品中心Cell Resources
联系我们CONTACT US
400-999-210024小时服务热线
人脑微血管内皮细胞
Human Brain Microvascular Endothelial Cells
货号：CP-H124
规格：5×10⁵Cells/T25培养瓶
价格： ￥8880
培养基货号： CM-H124
产品概述
人脑微血管内皮细胞
Cat NO.: CP-H124
-
产品名称：人脑微血管内皮细胞
-
英文简称：BMVECs
-
组织来源：脑组织
-
产品规格：5×10⁵Cells/T25培养瓶
-
细胞简介：
人脑微血管内皮细胞分离自脑组织；脑微血管内皮细胞是血脑屏障的主要组成成分，它是组成脑微血管腔面单层扁平上皮样细胞，能够限制可溶性物质和细胞等从血液进入大脑，大脑微血管内皮细胞与外周内皮细胞相比具有一些相同特性。它所产生和分泌的生物活性物质对维持血管张力、调节血压、抗血栓形成等有重要作用，在脑血管疾病的发病机制中有重要病理生理学意义。与外周内皮细胞相同，大脑微血管内皮细胞表面表达细胞粘附分子，调控白细胞进入大脑。由于微血管内皮细胞的器官特异性，内皮细胞通常取源于疾病研究的相关组织。其主要特征如下：①脑微血管内皮细胞存在许多细胞间紧密连接，产生很高的跨内皮阻抗，延迟细胞旁的通量；②脑微血管内皮细胞缺乏内皮细胞的窗孔结构，其液相物质胞饮水平较低；③脑微血管内皮细胞具有不对称定位酶和载体介导转运系统，从而产生“两极分化”的表现型。
-
方法简介：
普诺赛实验室分离的人脑微血管内皮细胞采用胶原酶-中性蛋白酶混合消化法结合密度梯度离心法、最后通过内皮细胞专用培养基培养筛选制备而来，细胞总量约为5×10⁵cells/瓶。
-
质量检测：
普诺赛实验室分离的人脑微血管内皮细胞经CD31免疫荧光鉴定，纯度可达90%以上，且不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等。
-
培养信息：
包被条件 PLL（0.1mg/ml）或明胶（0.1%） 培养基 含FBS、生长添加剂、Penicillin、Streptomycin等 产品货号 CM-H124 换液频率 每2-3天换液一次 生长特性 贴壁 细胞形态 内皮细胞样 传代特性 可传2-3代 消化液 0.25%胰蛋白酶 人脑微血管内皮细胞体外培养周期有限；建议使用普诺赛配套的专用生长培养基及正确的操作方法来培养，以此保证该细胞的最佳培养状态。
参考文献
-
IFITM3 promotes glioblastoma stem cell-mediated angiogenesis via regulating JAK/STAT3/bFGF signaling pathway (2024-01-13)
作者：Zhangsheng Xiong:1.Department of Neuro-oncological Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou, 510060, PR China. ; 2.Key Laboratory of Neurosurgery in Guangdong Province, Southern Medical University, Guangzhou, 510060, PR China. ; Xiangdong Xu:1.Department of Neuro-oncological Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou, 510060, PR China. ; 2.Key Laboratory of Neurosurgery in Guangdong Province, Southern Medical University, Guangzhou, 510060, PR China. ; Yuxuan Zhang:1.Department of Neuro-oncological Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou, 510060, PR China. ; 2.Key Laboratory of Neurosurgery in Guangdong Province, Southern Medical University, Guangzhou, 510060, PR China. ; 3.Department of Neurosurgery, Institute of Neuroscience, The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510260, PR China. ; Chengcheng Ma:1.Department of Neuro-oncological Surgery, Zhujiang Hospital, Southern Med
期刊：IFITM3 promotes glioblastoma stem cell-mediated angiogenesis via regulating JAK/STAT3/bFGF signaling pathway
DOI：10.1038/s41419-023-06416-5
影响因子 ：9.0
引用产品： 人脑微血管内皮细胞
-
Brain microvascular endothelial cell-derived exosomes transmitting circ_0000495 promote microglial M1-polarization and endothelial cell injury under hypoxia condition (2024-01-09)
作者：Xiao-Li Min:1.Department of Cerebrovascular Diseases, The Second Affiliated Hospital of Kunming Medical University, Kunming, China. ; Wen-Ji Jia:1.Department of Neurology, The Second Affiliated Hospital of Kunming Medical University, Kunming, China. ; Li Guo:1.Department of Radiology, The Second Affiliated Hospital of Kunming Medical University, Kunming, China. ; Rui Jing:1.Department of Cerebrovascular Diseases, The Second Affiliated Hospital of Kunming Medical University, Kunming, China. ; Xiao-Hong Zhao:1.Department of Cerebrovascular Diseases, The Second Affiliated Hospital of Kunming Medical University, Kunming, China. ; Jia-Yi Hu:1.Department of Cerebrovascular Diseases, The Second Affiliated Hospital of Kunming Medical University, Kunming, China. ; Xu-Hui Li:1.Department of Neurosurgery, The Second Affiliated Hospital of Kunming Medical University, Kunming, China. ; Wei Liu:1.Department of Anesthesiology, Huashan Hospital, Fudan University, Shanghai, China. ; Tao Wang:1.
期刊：Brain microvascular endothelial cell-derived exosomes transmitting circ_0000495 promote microglial M1-polarization and endothelial cell injury under hypoxia condition
影响因子 ：4.8
引用产品： 人脑微血管内皮细胞
-
Exosomal Mir-3613-3p derived from oxygen–glucose deprivation-treated brain microvascular endothelial cell promotes microglial M1 polarization (2023-03-05)
作者：Mengqi Zhang:1.Department of Neurology, Xiangya Hospital, Central South University, 87 Xiangya Road, Changsha, 410008, Hunan, China. zhangmengqi8912@163.com. ; 2.National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, 410008, China. zhangmengqi8912@163.com. ; Qian Wu:1.Department of Neurology, First Affiliated Hospital, Kunming Medical University, Kunming, 650032, China. ; Mimi Tang:1.Department of Pharmacy, Xiangya Hospital, Central South University, Changsha, 410008, China. ; 2.Institute of Hospital Pharmacy, Xiangya Hospital, Central South University, Changsha, 410008, China. ; Zhuohui Chen:1.Department of Neurology, Xiangya Hospital, Central South University, 87 Xiangya Road, Changsha, 410008, Hunan, China. ; 2.National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, 410008, China. ; Haiyue Wu:1.Department of Neurology, Xiangya Hospital, Central South University, 87
期刊：Exosomal Mir-3613-3p derived from oxygen–glucose deprivation-treated brain microvascular endothelial cell promotes microglial M1 polarization
DOI：10.1186/s11658-023-00432-1
影响因子 ：8.3
引用产品： 人脑微血管内皮细胞 , 人皮层神经元细胞 , 人皮层神经元细胞完全培养基
-
Stigmasterol protects human brain microvessel endothelial cells against ischemia-reperfusion injury through suppressing EPHA2 phosphorylation (2023-03-05)
作者：Suping Li:1.Department of Neurology, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, Chengdu 610072, China. ; Fei Xu:1.Department of Neurology, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, Chengdu 610072, China. ; Liang Yu:1.Department of Neurology, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, Chengdu 610072, China. ; Qian Yu:1.Department of Rehabilitation, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, Chengdu 610072, China. ; Nengwei Yu:1.Department of Neurology, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, Chengdu 610072, China. ; Jing Fu:1.Department of Rehabilitation, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, Chengdu 610072, China. Electronic address: 2091145921@qq.com. ;
期刊：Stigmasterol protects human brain microvessel endothelial cells against ischemia-reperfusion injury through suppressing EPHA2 phosphorylation
DOI：10.1016/S1875-5364(23)60390-5
影响因子 ：4.6
引用产品： 人脑微血管内皮细胞 , 人脑微血管内皮细胞完全培养基
-
Circ_0007706 downregulation ameliorates neonatal hypoxic ischemic encephalopathy via regulating the miR-579-3p/TRAF6 axis (2023-01-30)
作者：Jinguang Wang:1.Department of Neonatology, The Third Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China. Electronic address: 437537002@qq.com. ; Minmin Hua:1.Department of Neonatology, The Third Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China. Electronic address: 952756495@qq.com. ; Huixin Li:1.Department of Anesthesiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China. Electronic address: Lihuixin0321@163.com. ; Dan Xu:1.Department of Neurology, The Third Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China. Electronic address: 723932168@qq.com. ; Fangfang Li:1.Department of Respiratory Medicine, The Third Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China. Electronic address: Ff880319@163.com. ; Falin Xu:1.Department of Neonatology, The Third Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China. Electronic address: zdsfy123456@163.com. ;
期刊：Circ_0007706 downregulation ameliorates neonatal hypoxic ischemic encephalopathy via regulating the miR-579-3p/TRAF6 axis
DOI：10.1016/j.brainresbull.2023.01.013
影响因子 ：3.8
引用产品： 人脑微血管内皮细胞
-
Protective effects of brain and muscle ARNT-like gene 1 on oxidized low-density lipoprotein-induced human brain microvascular endothelial cell injury by alleviating ferroptosis (2023-06-21)
作者：Shui Yu:1.Department of Neurosurgery, The People's Hospital of Dujiangyan, Dujiangyan, Sichuan Province, China. ; Yijun Zeng:1.Department of Neurosurgery, The People's Hospital of Dujiangyan, Dujiangyan, Sichuan Province, China. ; Chenbin Ruan:1.Department of Neurology, The People's Hospital of Dujiangyan, Dujiangyan, Sichuan Province, China. ; Lei Bai:1.Department of Neurology, The People's Hospital of Dujiangyan, Dujiangyan, Sichuan Province, China. ; Zhang Liang:1.Department of Neurosurgery, The People's Hospital of Dujiangyan, Dujiangyan, Sichuan Province, China. ;
期刊：Protective effects of brain and muscle ARNT-like gene 1 on oxidized low-density lipoprotein-induced human brain microvascular endothelial cell injury by alleviating ferroptosis
影响因子 ：2.8
引用产品： 人脑微血管内皮细胞 , 人脑微血管内皮细胞完全培养基
-
Hsa_circ_0090002 regulates miR-186-5p/HECTD1 axis to mediate brain microvascular endothelial cell dysfunction (2011-01-20)
作者：Xiaoxia Sun:1.Department of Organ Transplantation, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan 250021, China. ; Mingying Dai:1.Department of Critical Care Medicine, the Affiliated Hospital of Qingdao University, Qingdao 266003, China. ; Xin Liu:1.Department of Critical Care Medicine, the Affiliated Hospital of Qingdao University, Qingdao 266003, China. ; Huimin Wang:1.Department of Critical Care Medicine, the Affiliated Hospital of Qingdao University, Qingdao 266003, China. ; Chuanlei Wang:1.Department of Critical Care Medicine, the Affiliated Hospital of Qingdao University, Qingdao 266003, China. ; Xinyi Fan:1.Department of Critical Care Medicine, the Affiliated Hospital of Qingdao University, Qingdao 266003, China. ; Wei Fang:1.Department of Critical Care Medicine, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan 250021, China. Electronic address: linaizuoan@163.com. ;
期刊：Hsa_circ_0090002 regulates miR-186-5p/HECTD1 axis to mediate brain microvascular endothelial cell dysfunction
DOI：10.1016/j.brainresbull.2021.11.007
影响因子 ：3.8
引用产品： 人脑微血管内皮细胞
-
Circ_0006768 upregulation attenuates oxygen–glucose deprivation/reoxygenation-induced human brain microvascular endothelial cell injuries by upregulating VEZF1 via miR-222-3p inhibition (2006-01-20)
作者：Jing Li:1.Department of Internal Medicine-Neurology, Hengshui People's Hospital, No. 180, Renmin East Road, Hengshui, 053000, China. ; Jiguang Wang:1.Department of Internal Medicine-Neurology, Hengshui People's Hospital, No. 180, Renmin East Road, Hengshui, 053000, China. wangjiguang0901@126.com. ; Zhi Wang:1.Department of Internal Medicine-Neurology, Hengshui People's Hospital, No. 180, Renmin East Road, Hengshui, 053000, China. ;
期刊：Circ_0006768 upregulation attenuates oxygen–glucose deprivation/reoxygenation-induced human brain microvascular endothelial cell injuries by upregulating VEZF1 via miR-222-3p inhibition
DOI：10.1007/s11011-021-00775-8
影响因子 ：3.6
引用产品： 人脑微血管内皮细胞
-
lncRNA HOTAIR mediates OGD/R‑induced cell injury and angiogenesis in a EZH2‑dependent manner (2012-01-20)
作者：Yunpeng Wang:1.Department of Neonatology, Huazhong University of Science and Technology Union Shenzhen Hospital, Shenzhen, Guangdong 518052, P.R. China. ; Jiaoyu Mao:1.Department of Neonatology, Huazhong University of Science and Technology Union Shenzhen Hospital, Shenzhen, Guangdong 518052, P.R. China. ; Xiaodong Li:1.Department of Neonatology, Huazhong University of Science and Technology Union Shenzhen Hospital, Shenzhen, Guangdong 518052, P.R. China. ; Beibei Wang:1.Department of Neonatology, Children's Hospital of Nanjing Medical University, Nanjing, Jiangsu 210008, P.R. China. ; Xiaoguang Zhou:1.Department of Neonatology, Children's Hospital of Nanjing Medical University, Nanjing, Jiangsu 210008, P.R. China. ;
期刊：lncRNA HOTAIR mediates OGD/R‑induced cell injury and angiogenesis in a EZH2‑dependent manner
影响因子 ：2.7
引用产品： 人脑微血管内皮细胞
-
Tetramethylpyrazine Protects Blood-Spinal Cord Barrier Integrity by Modulating Microglia Polarization Through Activation of STAT3/SOCS3 and Inhibition of NF- к B Signaling Pathways in Experimental Autoimmune Encephalomyelitis Mice (2020-05-18)
作者：Lianshuang Zhang:1.Department of Histology and Embryology, Binzhou Medical University, Yantai, 264003, People's Republic of China. ; Xueyan Lu:1.Department of Histology and Embryology, Binzhou Medical University, Yantai, 264003, People's Republic of China. ; Lihua Gong:1.Department of Histology and Embryology, Binzhou Medical University, Yantai, 264003, People's Republic of China. ; Linlu Cui:1.Department of Histology and Embryology, Binzhou Medical University, Yantai, 264003, People's Republic of China. ; Hongqin Zhang:1.Department of Histology and Embryology, Binzhou Medical University, Yantai, 264003, People's Republic of China. ; Wei Zhao:1.Department of Histology and Embryology, Binzhou Medical University, Yantai, 264003, People's Republic of China. ; Pengyu Jiang:1.Department of Histology and Embryology, Binzhou Medical University, Yantai, 264003, People's Republic of China. ; GuiGe Hou:1.The Key Laboratory of Prescription Effect and Clinical Evaluation of State Administra
期刊：Tetramethylpyrazine Protects Blood-Spinal Cord Barrier Integrity by Modulating Microglia Polarization Through Activation of STAT3/SOCS3 and Inhibition of NF- к B Signaling Pathways in Experimental Autoimmune Encephalomyelitis Mice
DOI：10.1007/s10571-020-00878-3
影响因子 ：4.0
引用产品： 人脑微血管内皮细胞
-
Asiaticoside Attenuates Cell Growth Inhibition and Apoptosis Induced by Aβ 1-42 via Inhibiting the TLR4/NF-κB Signaling Pathway in Human Brain Microvascular Endothelial Cells (2001-08-20)
作者：Daqiang Song:1.Department of Pharmacology, Southwest Medical University, Luzhou, China. ; Xian Jiang:1.Department of Anesthesiology, The Affiliated Hospital of Southwest Medical University, Luzhou, China. ; Yiliu Liu:1.Department of Pharmacology, Southwest Medical University, Luzhou, China. ; Yuhong Sun:1.Department of Pharmacology, Southwest Medical University, Luzhou, China. ; Shousong Cao:1.Department of Pharmacology, Southwest Medical University, Luzhou, China. ; Zhuo Zhang:1.Department of Pharmacology, Southwest Medical University, Luzhou, China. ;
期刊：Asiaticoside Attenuates Cell Growth Inhibition and Apoptosis Induced by Aβ 1-42 via Inhibiting the TLR4/NF-κB Signaling Pathway in Human Brain Microvascular Endothelial Cells
影响因子 ：5.6
引用产品： 人脑微血管内皮细胞
FAQs
Q：{{item.question}}
A：
产品资料
识别码示意图
