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人脑微血管内皮细胞
Human Brain Microvascular Endothelial Cells
货号:CP-H124
规格:5×10⁵Cells/T25培养瓶
价格: ¥8880
培养基货号: CM-H124
产品概述
人脑微血管内皮细胞
Cat NO.: CP-H124
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产品名称:人脑微血管内皮细胞
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英文简称:BMVECs
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组织来源:脑组织
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产品规格:5×10⁵Cells/T25培养瓶
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细胞简介:
人脑微血管内皮细胞分离自脑组织;脑微血管内皮细胞是血脑屏障的主要组成成分,它是组成脑微血管腔面单层扁平上皮样细胞,能够限制可溶性物质和细胞等从血液进入大脑,大脑微血管内皮细胞与外周内皮细胞相比具有一些相同特性。它所产生和分泌的生物活性物质对维持血管张力、调节血压、抗血栓形成等有重要作用,在脑血管疾病的发病机制中有重要病理生理学意义。与外周内皮细胞相同,大脑微血管内皮细胞表面表达细胞粘附分子,调控白细胞进入大脑。由于微血管内皮细胞的器官特异性,内皮细胞通常取源于疾病研究的相关组织。其主要特征如下:①脑微血管内皮细胞存在许多细胞间紧密连接,产生很高的跨内皮阻抗,延迟细胞旁的通量;②脑微血管内皮细胞缺乏内皮细胞的窗孔结构,其液相物质胞饮水平较低;③脑微血管内皮细胞具有不对称定位酶和载体介导转运系统,从而产生“两极分化”的表现型。
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方法简介:
普诺赛实验室分离的人脑微血管内皮细胞采用胶原酶-中性蛋白酶混合消化法结合密度梯度离心法、最后通过内皮细胞专用培养基培养筛选制备而来,细胞总量约为5×10⁵cells/瓶。
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质量检测:
普诺赛实验室分离的人脑微血管内皮细胞经CD31免疫荧光鉴定,纯度可达90%以上,且不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等。
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培养信息:
包被条件 PLL(0.1mg/ml)或明胶(0.1%) 培养基 含FBS、生长添加剂、Penicillin、Streptomycin等 产品货号 CM-H124 换液频率 每2-3天换液一次 生长特性 贴壁 细胞形态 内皮细胞样 传代特性 可传2-3代 消化液 0.25%胰蛋白酶 人脑微血管内皮细胞体外培养周期有限;建议使用普诺赛配套的专用生长培养基及正确的操作方法来培养,以此保证该细胞的最佳培养状态。
参考文献
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IFITM3 promotes glioblastoma stem cell-mediated angiogenesis via regulating JAK/STAT3/bFGF signaling pathway (2024-01-13)
期刊:Cell Death & Disease
DOI:10.1038/s41419-023-06416-5
影响因子 :9.0
引用产品: 人脑微血管内皮细胞
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Deficiency of the flagellin subunit FliC exacerbates the pathogenicity of extraintestinal pathogenic Escherichia coli in BALB/c mice by inducing a more intense inflammation (2024-12-16)
期刊:INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
DOI:10.1016/j.ijbiomac.2024.138761
影响因子 :7.7
引用产品: 人脑微血管内皮细胞 , RAW 264.7 细胞
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Silencing BMAL1 promotes M1/M2 polarization through the LDHA/lactate axis to promote GBM sensitivity to bevacizumab (2024-05-10)
期刊:INTERNATIONAL IMMUNOPHARMACOLOGY
DOI:10.1016/j.intimp.2024.112187
影响因子 :4.8
引用产品: RAW 264.7 细胞 , 人脑微血管内皮细胞
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Brain microvascular endothelial cell-derived exosomes transmitting circ_0000495 promote microglial M1-polarization and endothelial cell injury under hypoxia condition (2024-01-09)
期刊:FASEB JOURNAL
影响因子 :4.8
引用产品: 人脑微血管内皮细胞
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Astrocytes-Secreted WNT5B Disrupts the Blood–Brain Barrier Via ROR1/JNK/c-JUN Cascade During Meningitic Escherichia Coli Infection (2024-06-19)
期刊:MOLECULAR NEUROBIOLOGY
DOI:10.1007/s12035-024-04303-4
影响因子 :4.6
引用产品: U251 细胞 , 人脑微血管内皮细胞 , 293T [HEK-293T] 细胞
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Irisflorentin improves functional recovery after spinal cord injury by protecting the blood–spinal cord barrier and promoting axonal growth (2024-07-10)
期刊:EXPERIMENTAL NEUROLOGY
DOI:10.1016/j.expneurol.2024.114886
影响因子 :4.6
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Intervention of exogenous VEGF protect brain microvascular endothelial cells from hypoxia-induced injury by regulating PLCγ/RAS/ERK and PI3K/AKT pathways (2024-05-21)
期刊:EXPERIMENTAL GERONTOLOGY
DOI:10.1016/j.exger.2024.112452
影响因子 :3.9
引用产品: 人脑微血管内皮细胞完全培养基 , 人脑微血管内皮细胞
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Asiaticoside protected brain injury in hypertensive intracerebral hemorrhage via activation of the PI3K/AKT pathway (2024-09-10)
期刊:JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
影响因子 :3.2
引用产品: 人脑微血管内皮细胞完全培养基 , 人脑微血管内皮细胞
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Paraquat disrupts the blood–brain barrier by increasing IL-6 expression and oxidative stress through the activation of PI3K/AKT signaling pathway (2024-09-11)
期刊:Open Medicine
影响因子 :1.7
引用产品: 人脑微血管内皮细胞
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Exosomal Mir-3613-3p derived from oxygen–glucose deprivation-treated brain microvascular endothelial cell promotes microglial M1 polarization (2023-03-05)
期刊:CELLULAR & MOLECULAR BIOLOGY LETTERS
DOI:10.1186/s11658-023-00432-1
影响因子 :8.3
引用产品: 人脑微血管内皮细胞 , 人皮层神经元细胞 , 人皮层神经元细胞完全培养基
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Stigmasterol protects human brain microvessel endothelial cells against ischemia-reperfusion injury through suppressing EPHA2 phosphorylation (2023-03-05)
期刊:Chinese Journal of Natural Medicines
DOI:10.1016/S1875-5364(23)60390-5
影响因子 :4.6
引用产品: 人脑微血管内皮细胞 , 人脑微血管内皮细胞完全培养基
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Circ_0007706 downregulation ameliorates neonatal hypoxic ischemic encephalopathy via regulating the miR-579-3p/TRAF6 axis (2023-01-30)
期刊:BRAIN RESEARCH BULLETIN
DOI:10.1016/j.brainresbull.2023.01.013
影响因子 :3.8
引用产品: 人脑微血管内皮细胞
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Heat-shock protein A12A attenuates oxygen-glucose deprivation/reoxygenation-induced human brain microvascular endothelial cell dysfunction via PGC-1α/SIRT3 pathway (2023-11-09)
期刊:DRUG DEVELOPMENT RESEARCH
影响因子 :3.8
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Protective effects of brain and muscle ARNT-like gene 1 on oxidized low-density lipoprotein-induced human brain microvascular endothelial cell injury by alleviating ferroptosis (2023-06-21)
期刊:HUMAN & EXPERIMENTAL TOXICOLOGY
影响因子 :2.8
引用产品: 人脑微血管内皮细胞 , 人脑微血管内皮细胞完全培养基
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Exosomal lncRNA LINC01356 Derived From Brain Metastatic Nonsmall-Cell Lung Cancer Cells Remodels the Blood-Brain Barrier (2022-04-27)
期刊:Frontiers in Oncology
影响因子 :5.7
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Hsa_circ_0090002 regulates miR-186-5p/HECTD1 axis to mediate brain microvascular endothelial cell dysfunction (2021-11-18)
期刊:Brain Research Bulletin
DOI:10.1016/j.brainresbull.2021.11.007
影响因子 :3.8
引用产品: 人脑微血管内皮细胞
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Circ_0006768 upregulation attenuates oxygen–glucose deprivation/reoxygenation-induced human brain microvascular endothelial cell injuries by upregulating VEZF1 via miR-222-3p inhibition (2021-06-19)
期刊:Metabolic Brain Disease
DOI:10.1007/s11011-021-00775-8
影响因子 :3.6
引用产品: 人脑微血管内皮细胞
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lncRNA HOTAIR mediates OGD/R?induced cell injury and angiogenesis in a EZH2?dependent manner (2021-12-01)
期刊:Experimental and Therapeutic Medicine
影响因子 :2.7
引用产品: 人脑微血管内皮细胞
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Tetramethylpyrazine Protects Blood-Spinal Cord Barrier Integrity by Modulating Microglia Polarization Through Activation of STAT3/SOCS3 and Inhibition of NF- к B Signaling Pathways in Experimental Autoimmune Encephalomyelitis Mice (2020-05-18)
期刊:Cellular and Molecular Neurobiology
DOI:10.1007/s10571-020-00878-3
影响因子 :4.0
引用产品: 人脑微血管内皮细胞
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Asiaticoside Attenuates Cell Growth Inhibition and Apoptosis Induced by Aβ 1-42 via Inhibiting the TLR4/NF-κB Signaling Pathway in Human Brain Microvascular Endothelial Cells (2018-01-03)
期刊:Frontiers in Pharmacology
影响因子 :5.6
引用产品: 人脑微血管内皮细胞
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