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大鼠肺微血管内皮细胞
Rat Pulmonary Microvascular Endothelial Cells
货号：CP-R001
规格：5×10⁵Cells/T25培养瓶
价格： ￥3380
培养基货号： CM-R001
产品概述
大鼠肺微血管内皮细胞
Cat NO.: CP-R001
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产品名称：大鼠肺微血管内皮细胞
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组织来源：肺组织
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产品规格：5×10⁵Cells/T25培养瓶
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细胞简介：
大鼠肺微血管内皮细胞分离自肺组织；肺是机体的呼吸器官，位于胸腔，左右各一，覆盖于心之上。肺有分叶，左二右三，共五叶。肺经肺系(指气管、支气管等)与喉、鼻相连，故称喉为肺之门户，鼻为肺之外窍。微血管内皮细胞密切参与包括再生、发育、伤口愈合等一系列生理及炎症反应。细胞呈梭形或多角形，形成单层后呈鹅卵石样或铺路石样排列。肺微血管内皮细胞构成半选择性屏障，该屏障对于肺气体交换，调节液体和可溶物在血液与肺间质之间的流动具有重要意义。
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方法简介：
普诺赛实验室分离的大鼠肺微血管内皮细胞采用组织贴块法并结合内皮细胞专用培养基培养筛选制备而来，细胞总量约为5×10⁵cells/瓶。
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质量检测：
普诺赛实验室分离的大鼠肺微血管内皮细胞经CD31免疫荧光鉴定，纯度可达90%以上，且不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等。
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培养信息：
包被条件 PLL（0.1mg/ml）或明胶（0.1%） 培养基 含FBS、生长添加剂、Penicillin、Streptomycin等 产品货号 CM-R001 换液频率 每2-3天换液一次 生长特性 贴壁 细胞形态 内皮细胞样 传代特性 可传2-3代 消化液 0.25%胰蛋白酶 大鼠肺微血管内皮细胞体外培养周期有限；建议使用普诺赛配套的专用生长培养基及正确的操作方法来培养，以此保证该细胞的最佳培养状态。
参考文献
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Sema3A inactivates the ERK/JNK signalling pathways to alleviate inflammation and oxidative stress in lipopolysaccharide-stimulated rat endothelial cells and lung tissues (2023-04-26)
作者：Qianwen Qiu:1.Department of Medical Ultrasonics, Lishui People's Hospital, Lishui, Zhejiang, P.R. China. ; Xiufeng Yu:1.Department of Emergency Medicine, Lishui People's Hospital, Lishui, Zhejiang, P.R. China. ; Qingli Chen:1.Department of Emergency Medicine, Lishui People's Hospital, Lishui, Zhejiang, P.R. China. ; Xuwei He:1.Department of Emergency Medicine, Lishui People's Hospital, Lishui, Zhejiang, P.R. China. ;
期刊：Sema3A inactivates the ERK/JNK signalling pathways to alleviate inflammation and oxidative stress in lipopolysaccharide-stimulated rat endothelial cells and lung tissues
DOI：10.1080/08916934.2023.2200908
影响因子 ：3.5
引用产品： 大鼠肺微血管内皮细胞 , 大鼠肺微血管内皮细胞完全培养基 , Annexin V-FITC/ PI荧光双染细胞凋亡检测试剂盒
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Transcription Factor p300 Regulated miR-451b Weakens the Cigarette Smoke Extract-Induced Cellular Stress by Targeting RhoA/ROCK2 Signaling (2022-10-14)
作者：Wen Shen:1.Department of Respiratory Medicine, The Second Affiliated Hospital of Kunming Medical University, Kunming, China. ; Shukun Wang:1.Department of Respiratory Medicine, The Second Affiliated Hospital of Kunming Medical University, Kunming, China. ; Ruili Wang:1.Department of Respiratory Medicine, The Second Affiliated Hospital of Kunming Medical University, Kunming, China. ; Yang Zhang:1.Department of Respiratory Medicine, The Second Affiliated Hospital of Kunming Medical University, Kunming, China. ; Hong Tian:1.Department of Respiratory Medicine, The Second Affiliated Hospital of Kunming Medical University, Kunming, China. ; Xi Wang:1.Department of Respiratory Medicine, The Second Affiliated Hospital of Kunming Medical University, Kunming, China. ; Xin Wu:1.Department of Respiratory Medicine, The Second Affiliated Hospital of Kunming Medical University, Kunming, China. ; Xiaolei Yang:1.Department of Respiratory Medicine, The Second Affiliated Hospital of Kunming Medica
期刊：Transcription Factor p300 Regulated miR-451b Weakens the Cigarette Smoke Extract-Induced Cellular Stress by Targeting RhoA/ROCK2 Signaling
影响因子 ：7.3
引用产品： RAW 264.7 细胞 , 大鼠肺微血管内皮细胞 , L2 细胞 , 大鼠支气管上皮细胞 , 大鼠肺成纤维细胞
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Adipose-derived exosomes protect the pulmonary endothelial barrier in ventilator-induced lung injury by inhibiting the TRPV4/Ca2+ signaling pathway (2002-00-20)
作者：Qian Yu:1.Department of Respiratory Medicine, Second Affiliated Hospital of Chongqing Medical University, Chongqing, China. ; Daoxin Wang:1.Department of Respiratory Medicine, Second Affiliated Hospital of Chongqing Medical University, Chongqing, China. ; Xiaoting Wen:1.Department of Respiratory Medicine, Second Affiliated Hospital of Chongqing Medical University, Chongqing, China. ; Xumao Tang:1.Department of Respiratory Medicine, Second Affiliated Hospital of Chongqing Medical University, Chongqing, China. ; Di Qi:1.Department of Respiratory Medicine, Second Affiliated Hospital of Chongqing Medical University, Chongqing, China. ; Jing He:1.Department of Respiratory Medicine, Second Affiliated Hospital of Chongqing Medical University, Chongqing, China. ; Yan Zhao:1.Department of Respiratory Medicine, Second Affiliated Hospital of Chongqing Medical University, Chongqing, China. ; Wang Deng:1.Department of Respiratory Medicine, Second Affiliated Hospital of Chongqing Medical Univ
期刊：Adipose-derived exosomes protect the pulmonary endothelial barrier in ventilator-induced lung injury by inhibiting the TRPV4/Ca2+ signaling pathway
DOI：10.1152/ajplung.00255.2019
影响因子 ：4.9
引用产品： 大鼠肺微血管内皮细胞
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