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产品概述
大鼠肺成纤维细胞
Cat NO.: CP-R006
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产品名称：大鼠肺成纤维细胞
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英文简称：LF
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组织来源：肺组织
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产品规格：5×10⁵Cells/T25培养瓶
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细胞简介：
大鼠肺成纤维细胞分离自肺组织；肺是机体的呼吸器官，位于胸腔，左右各一，覆盖于心之上。肺有分叶，左二右三，共五叶。肺经肺系(指气管、支气管等)与喉、鼻相连，故称喉为肺之门户，鼻为肺之外窍。成纤维细胞(Fibroblast)是疏松结缔组织的主要细胞成分，由胚胎时期的间充质细胞分化而来；成纤维细胞较大，轮廓清楚，多为突起的纺锤形或星形的扁平状结构，其细胞核呈规则的卵圆形，核仁大而明显。成纤维细胞功能活动旺盛，细胞质嗜弱碱性，具明显的蛋白质合成和分泌活动，在一定条件下，它可以实现跟纤维细胞的互相转化；成纤维细胞对不同程度的细胞变性、坏死和组织缺损的修复有着十分重要的作用。刚分离的肺成纤维细胞呈圆形、折光性良好，悬浮于培养基中。30min细胞贴壁，其中部分开始伸出伪足，表现为小的突起；6h后细胞基本贴壁完全，伸展成梭形，胞核清晰，分布较均匀，散在生长，不聚集成团；细胞生长迅速，5-7天即呈融合状态，细胞排列紧密，有的交叉重叠生长，平坦、胞体较大，细胞质透明，细胞核较大，呈椭圆形，颜色淡。细胞融合，并彼此连接成网状；细胞呈突起的纺锤形或星形的扁平分布。肺成纤维细胞在生理条件下的主要功能包括：构造和维持肺器官的正常形态，合成和释放细胞外基质以及组织损伤后及时大量聚集修复损伤组织。
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方法简介：
普诺赛实验室分离的大鼠肺成纤维细胞采用胰蛋白酶 - 胶原酶混合消化法结合差速贴壁法制备而来，细胞总量约为5×10⁵cells/瓶。
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质量检测：
普诺赛实验室分离的大鼠肺成纤维细胞经Vimentin免疫荧光鉴定，纯度可达90%以上，且不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等。
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培养信息：
包被条件 培养基 含FBS、生长添加剂、Penicillin、Streptomycin等 产品货号 CM-R006 换液频率 每2-3天换液一次 生长特性 贴壁 细胞形态 成纤维细胞样 传代特性 可传5代左右；3代以内状态最佳 消化液 0.25%胰蛋白酶 大鼠肺成纤维细胞体外培养周期有限；建议使用普诺赛配套的专用生长培养基及正确的操作方法来培养，以此保证该细胞的最佳培养状态。
参考文献
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The mechanism of Shenlong Jianji treatment of idiopathic pulmonary fibrosis inhibits fibroblast-to-myofibroblast transformation via the TGF-β1/smads signaling pathway (2023-12-18)
作者：Jiaxiang Pan:1.The Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, 33 Beiling Street, Shenyang, 110032, Liaoning, China. Electronic address: 953964919@qq.com. ; Yue Li:1.The Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, 33 Beiling Street, Shenyang, 110032, Liaoning, China. Electronic address: med_liyue@163.com. ; Xize Wu:1.Liaoning University of Traditional Chinese Medicine, 79 East of Chongshan Road, Shenyang, 110847, Liaoning, China; Nantong Hospital of Traditional Chinese Medicine, Nantong Hospital Affiliated to Nanjing University of Chinese Medicine, Nantong, 226000, Jiangsu, China. Electronic address: 953935269@qq.com. ; Xue Pan:1.Liaoning University of Traditional Chinese Medicine, 79 East of Chongshan Road, Shenyang, 110847, Liaoning, China; Dazhou Vocational College of Chinese Medicine, Dazhou, 635000, Sichuan, China. Electronic address: 245067815@qq.com. ; Chuang Liu:1.The Affiliated Hospital of Liaoning University
期刊：The mechanism of Shenlong Jianji treatment of idiopathic pulmonary fibrosis inhibits fibroblast-to-myofibroblast transformation via the TGF-β1/smads signaling pathway
影响因子 ：5.4
引用产品： 大鼠肺成纤维细胞完全培养基 , 大鼠肺成纤维细胞
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LncRNA TUG1 promotes pulmonary fibrosis progression via up-regulating CDC27 and activating PI3K/Akt/mTOR pathway (2023-03-30)
作者：Fei Qi:1.Department of Pulmonary and Critical Care Medicine, Peking University Shenzhen Hospital, Shenzhen Peking University-The Hong Kong University of Science and Technology Medical Center, Shenzhen, P.R. China. ; Zhong-Dong Lv:1.Department of Pulmonary and Critical Care Medicine, Peking University Shenzhen Hospital, Shenzhen, P.R. China. ; Wen-Di Huang:1.Department of Pulmonary and Critical Care Medicine, Peking University Shenzhen Hospital, Shenzhen, P.R. China. ; Shan-Chen Wei:1.Department of Geriatrics, Peking University First Hospital, Beijing, P.R. China. ; Xin-Min Liu:1.Department of Geriatrics, Peking University First Hospital, Beijing, P.R. China. ; Wei-Dong Song:1.Department of Pulmonary and Critical Care Medicine, Peking University Shenzhen Hospital, Shenzhen, P.R. China. ;
期刊：LncRNA TUG1 promotes pulmonary fibrosis progression via up-regulating CDC27 and activating PI3K/Akt/mTOR pathway
DOI：10.1080/15592294.2023.2195305
影响因子 ：3.7
引用产品： 大鼠肺成纤维细胞
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Transcription Factor p300 Regulated miR-451b Weakens the Cigarette Smoke Extract-Induced Cellular Stress by Targeting RhoA/ROCK2 Signaling (2022-10-14)
作者：Wen Shen:1.Department of Respiratory Medicine, The Second Affiliated Hospital of Kunming Medical University, Kunming, China. ; Shukun Wang:1.Department of Respiratory Medicine, The Second Affiliated Hospital of Kunming Medical University, Kunming, China. ; Ruili Wang:1.Department of Respiratory Medicine, The Second Affiliated Hospital of Kunming Medical University, Kunming, China. ; Yang Zhang:1.Department of Respiratory Medicine, The Second Affiliated Hospital of Kunming Medical University, Kunming, China. ; Hong Tian:1.Department of Respiratory Medicine, The Second Affiliated Hospital of Kunming Medical University, Kunming, China. ; Xi Wang:1.Department of Respiratory Medicine, The Second Affiliated Hospital of Kunming Medical University, Kunming, China. ; Xin Wu:1.Department of Respiratory Medicine, The Second Affiliated Hospital of Kunming Medical University, Kunming, China. ; Xiaolei Yang:1.Department of Respiratory Medicine, The Second Affiliated Hospital of Kunming Medica
期刊：Transcription Factor p300 Regulated miR-451b Weakens the Cigarette Smoke Extract-Induced Cellular Stress by Targeting RhoA/ROCK2 Signaling
影响因子 ：7.3
引用产品： RAW 264.7 细胞 , 大鼠肺微血管内皮细胞 , L2 细胞 , 大鼠支气管上皮细胞 , 大鼠肺成纤维细胞
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