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产品概述
小鼠肝实质细胞
Cat NO.: CP-M033
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产品名称：小鼠肝实质细胞
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组织来源：肝脏组织
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产品规格：5×10⁵Cells/T25培养瓶
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细胞简介：
小鼠肝实质细胞分离自肝脏组织；肝脏是身体内以代谢功能为主的一个器官，并在身体里面起着去氧化、储存肝糖、分泌性蛋白质的合成等作用；肝脏也制造消化系统中之胆汁。肝脏是机体内脏里最大的器官，位于机体中的腹部位置，在右侧横隔膜之下，位于胆囊之前端且于右边肾脏的前方，胃的上方。肝脏是机体消化系统中最大的消化腺，为一红棕色的V字形器官。肝脏是尿素合成的主要器官，又是新陈代谢的重要器官。肝脏在机体位置和形态结构：肝脏位于右上腹，隐藏在右侧膈下和肋骨深面，大部分肝为肋弓所复盖，仅在腹上区、右肋弓间露出并直接接触腹前壁，肝上面则与膈及腹前壁相接。肝实质细胞是指具有肝功能的单位，是肝脏的基本组成单位之一。肝实质细胞与主要病生理变化：急性肝炎、肝硬化、肝脓肿。肝实质细胞属于中高度分化细胞，生长营养需求高，在体外存活时间短；细胞呈圆形或多角形，核大而圆，居中，常染色质丰富，部分有双核或多倍体核。肝脏作为体内重要的器官，在整个物质代谢过程中具有广泛而多样的作用。
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方法简介：
普诺赛实验室分离的小鼠肝实质细胞采用混合酶灌流消化、反复低速离心制备而来，细胞总量约为5×10⁵cells/瓶。
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质量检测：
普诺赛实验室分离的小鼠肝实质细胞经Cytokeratin-18免疫荧光鉴定，纯度可达90%以上，且不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等。
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培养信息：
包被条件 鼠尾胶原Ⅰ(2-5μg/cm2) 培养基 含FBS、生长添加剂、Penicillin、Streptomycin等 产品货号 CM-M033 换液频率 每2-3天换液一次 生长特性 贴壁 细胞形态 上皮细胞样 传代特性 可传1代，不建议传代 消化液 0.25%胰蛋白酶 小鼠肝实质细胞体外培养周期有限；建议使用普诺赛配套的专用生长培养基及正确的操作方法来培养，以此保证该细胞的最佳培养状态。
参考文献
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Splenectomy ameliorates liver cirrhosis by restoring the gut microbiota balance (2024-01-12)
作者：Ye Jin:1.Department of General Surgery, Key Laboratory of Hepatosplenic Surgery, Ministry of Education, The First Affiliated Hospital of Harbin Medical University, Harbin, 150081, Heilongjiang, People's Republic of China. hydjinye@hotmail.com. ; Meixin Shi:1.Department of Pathophysiology, Harbin Medical University, Harbin, 150086, Heilongjiang, People's Republic of China. ; Jing Feng:1.Department of General Surgery, Key Laboratory of Hepatosplenic Surgery, Ministry of Education, The First Affiliated Hospital of Harbin Medical University, Harbin, 150081, Heilongjiang, People's Republic of China. ; Zhengwei Zhang:1.Department of General Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, 150086, Heilongjiang, People's Republic of China. ; Bingbing Zhao:1.Department of Pathophysiology, Harbin Medical University, Harbin, 150086, Heilongjiang, People's Republic of China. ; Qingyu Li:1.Department of General Surgery, Key Laboratory of Hepatosplenic Surgery, Mini
期刊：Splenectomy ameliorates liver cirrhosis by restoring the gut microbiota balance
DOI：10.1007/s00018-023-05055-5
影响因子 ：8.0
引用产品： 小鼠肝实质细胞 , 小鼠肝星状细胞 , 小鼠肝实质细胞完全培养基 , 小鼠肝星状细胞完全培养基
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ETNPPL modulates hyperinsulinemia-induced insulin resistance through the SIK1/ROS-mediated inactivation of the PI3K/AKT signaling pathway in hepatocytes (2023-03-16)
作者：Xueyi Chen:1.Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Faculty of Life Sciences and Medicine, Northwest University, Shaanxi, Xi'an, China. ; Ping Liu:1.Department of Endocrinology, Xi'an No. 3 Hospital, The Affiliated Hospital of Northwest University, Northwest University, Shaanxi, Xi'an, P.R. China. ; Wei Zhang:1.Department of Endocrinology, Xi'an No. 3 Hospital, The Affiliated Hospital of Northwest University, Northwest University, Shaanxi, Xi'an, P.R. China. ; Xiaofang Li:1.Department of Gastroenterology, Xi'an No. 3 Hospital, The Affiliated Hospital of Northwest University, Northwest University, Shaanxi, Xi'an, P.R. China. ; Caihua Wang:1.Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Faculty of Life Sciences and Medicine, Northwest University, Shaanxi, Xi'an, China. ; Feifei Han:1.Department of Endocrinology, Xi'an No. 3 Hospital, The Affiliated Hospital of Northwest University,
期刊：ETNPPL modulates hyperinsulinemia-induced insulin resistance through the SIK1/ROS-mediated inactivation of the PI3K/AKT signaling pathway in hepatocytes
影响因子 ：5.6
引用产品： 小鼠肝实质细胞 , Hep G2 细胞 , 293T [HEK-293T] 细胞
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