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产品概述
小鼠肝星状细胞
Cat NO.: CP-M041
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产品名称：小鼠肝星状细胞
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组织来源：肝脏组织
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产品规格：5×10⁵Cells/T25培养瓶
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细胞简介：
小鼠肝星形细胞分离自肝脏组织；肝脏是身体内以代谢功能为主的一个器官，并在身体里面起着去氧化、储存肝糖、分泌性蛋白质的合成等作用；肝脏也制造消化系统中之胆汁。肝脏是机体内脏里最大的器官，位于机体中的腹部位置，在右侧横隔膜之下，位于胆囊之前端且于右边肾脏的前方，胃的上方。肝脏是机体消化系统中最大的消化腺，为一红棕色的V字形器官。肝脏是尿素合成的主要器官，又是新陈代谢的重要器官。肝脏在机体位置和形态结构：肝脏位于右上腹，隐藏在右侧膈下和肋骨深面，大部分肝为肋弓所复盖，仅在腹上区、右肋弓间露出并直接接触腹前壁，肝上面则与膈及腹前壁相接。肝星形细胞(HSC)又名肝贮脂细胞、维生素A贮存细胞、窦周细胞、Ito细胞等，是肝脏细胞外基质的主要来源。HSC在激活后可进一步转化为肌成纤维细胞样细胞，各种可导致肝纤维化的因素均将HSC作为最终靶细胞。正常情况下，HSC处于静止状态，当肝脏发生炎症反应或受到机械刺激等损伤时，HSC的表型由静止型转变为激活型，激活的HSC可通过增生和分泌细胞外基质参与肝纤维化的形成及肝内结构的重建，此过程也是肝纤维化的中心环节。肝星形细胞分布于肝脏的Disse间隙内，是合成、分泌细胞外基质的主要来源，在肝纤维化的发生中处于最重要地位。肝星形细胞是肝脏特有的间充质细胞，约占肝脏细胞总数的8%-13%。作为肝脏合成细胞外基质的主要细胞群，肝星形细胞不仅能分泌蛋白多糖、糖蛋白等细胞外基质成分，合成一定量的胶原酶以维持正常的基底膜结构，还能通过其突起的收缩参与肝窦的微循环调节。此外，肝星形细胞能通过合成肝细胞生长因子、胰岛素样生长因子、表皮生长因子等促进肝细胞增殖和肝再生。
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方法简介：
普诺赛实验室分离的小鼠肝星形细胞采用混合酶灌流消化、低速离心、密度梯度离心制备而来，细胞总量约为5×10⁵cells/瓶。
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质量检测：
普诺赛实验室分离的小鼠肝星状细胞经α-SMA或Desmin免疫荧光鉴定，纯度可达90%以上，且不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等。
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培养信息：
包被条件 培养基 含FBS、生长添加剂、Penicillin、Streptomycin等 产品货号 CM-M041 换液频率 每2-3天换液一次 生长特性 贴壁 细胞形态 成纤维细胞样 传代特性 可传3代左右 消化液 0.25%胰蛋白酶 小鼠肝星状细胞体外培养周期有限；建议使用普诺赛配套的专用生长培养基及正确的操作方法来培养，以此保证该细胞的最佳培养状态。
参考文献
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Splenectomy ameliorates liver cirrhosis by restoring the gut microbiota balance (2024-01-12)
作者：Ye Jin:1.Department of General Surgery, Key Laboratory of Hepatosplenic Surgery, Ministry of Education, The First Affiliated Hospital of Harbin Medical University, Harbin, 150081, Heilongjiang, People's Republic of China. hydjinye@hotmail.com. ; Meixin Shi:1.Department of Pathophysiology, Harbin Medical University, Harbin, 150086, Heilongjiang, People's Republic of China. ; Jing Feng:1.Department of General Surgery, Key Laboratory of Hepatosplenic Surgery, Ministry of Education, The First Affiliated Hospital of Harbin Medical University, Harbin, 150081, Heilongjiang, People's Republic of China. ; Zhengwei Zhang:1.Department of General Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, 150086, Heilongjiang, People's Republic of China. ; Bingbing Zhao:1.Department of Pathophysiology, Harbin Medical University, Harbin, 150086, Heilongjiang, People's Republic of China. ; Qingyu Li:1.Department of General Surgery, Key Laboratory of Hepatosplenic Surgery, Mini
期刊：Splenectomy ameliorates liver cirrhosis by restoring the gut microbiota balance
DOI：10.1007/s00018-023-05055-5
影响因子 ：8.0
引用产品： 小鼠肝实质细胞 , 小鼠肝星状细胞 , 小鼠肝实质细胞完全培养基 , 小鼠肝星状细胞完全培养基
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Hepatocyte-derived exosomes deliver H2AFJ to hepatic stellate cells and promote liver fibrosis via the MAPK/STMN1 axis activation (2023-01-04)
作者：Bin Liu:1.Department of Critical Care Medicine, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan 250021, PR China. ; Jinchao Wang:1.Department of Neurosurgery, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan 250021, PR China. ; Guangchuan Wang:1.Department of Gastroenterology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan 250021, PR China. ; Wanli Jiang:1.First Clinical School of Medicine, Shandong University of Traditional Chinese Medicine, Jinan 250355, PR China. ; Zhen Li:1.Department of Gastroenterology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan 250021, PR China. ; Yongjun Shi:1.Department of Gastroenterology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan 250021, PR China. ; Junyong Zhang:1.Department of Gastroenterology, Shandong Provincial Hospital Affiliated to Shandong First Medical Uni
期刊：Hepatocyte-derived exosomes deliver H2AFJ to hepatic stellate cells and promote liver fibrosis via the MAPK/STMN1 axis activation
DOI：10.1016/j.intimp.2022.109605
影响因子 ：5.6
引用产品： AML12 细胞 , 青霉素-链霉素溶液 (双抗)，100× , DMEM/F12 培养基 , 小鼠肝星状细胞 , 小鼠肝星状细胞完全培养基
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ADSCs-derived exosomes ameliorate hepatic fibrosis by suppressing stellate cell activation and remodeling hepatocellular glutamine synthetase-mediated glutamine and ammonia homeostasis (2022-10-04)
作者：Baitong Wu:1.East Hospital, School of Medicine, Tongji University, Shanghai, 200120, People's Republic of China. ; Jiuxing Feng:1.Key Laboratory of Medical Epigenetics and Metabolism, Institutes of Biomedical Sciences, Fudan University, Shanghai, People's Republic of China. ; Jingyi Guo:1.East Hospital, School of Medicine, Tongji University, Shanghai, 200120, People's Republic of China. ; Jian Wang:1.East Hospital, School of Medicine, Tongji University, Shanghai, 200120, People's Republic of China. ; Guanghui Xiu:1.Department of Intensive Care Unit, Affiliated Hospital of Yunnan University (The Second People's Hospital of Yunnan Province), Yunnan University, Kunming, People's Republic of China. ; Jiaqi Xu:1.East Hospital, School of Medicine, Tongji University, Shanghai, 200120, People's Republic of China. ; Ke Ning:1.Department of Neuroscience, Sheffield Institute for Translational Neuroscience, University of Sheffield, Sheffield, UK. ; Bin Ling:1.Department of Intensive Care Un
期刊：ADSCs-derived exosomes ameliorate hepatic fibrosis by suppressing stellate cell activation and remodeling hepatocellular glutamine synthetase-mediated glutamine and ammonia homeostasis
DOI：10.1186/s13287-022-03049-x
影响因子 ：8.1
引用产品： AML12 细胞 , 小鼠肝星状细胞 , 小鼠肝星状细胞完全培养基
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Myrrhone inhibits the progression of hepatic fibrosis by regulating the abnormal activation of hepatic stellate cells (2022-08-19)
作者：Zhangchi Sun:1.Pharmacy Department, Zhejiang Rongjun Hospital, Jiaxing, China. ; Xiaolan Zhan:1.Pharmacy Department, Zhejiang Rongjun Hospital, Jiaxing, China. ;
期刊：Myrrhone inhibits the progression of hepatic fibrosis by regulating the abnormal activation of hepatic stellate cells
影响因子 ：3.6
引用产品： 小鼠肝星状细胞
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Ppic modulates CCl4-induced liver fibrosis and TGF-β-caused mouse hepatic stellate cell activation and regulated by miR-137-3p (2007-01-20)
作者：Xin Yang:1.Department of General Surgery, The Second Xiangya Hospital, Central South University, Changsha, Hunan, 410011, China. ; Bo Shu:1.Department of General Surgery, The Second Xiangya Hospital, Central South University, Changsha, Hunan, 410011, China. ; Yingxia Zhou:1.Department of Surgery, The Second Xiangya Hospital, Central South University, Changsha, Hunan, 410011, China. ; Zhuan Li:1.Key Laboratory of Study and Discovery of Small Targeted Molecules of Hunan Province, Hunan Normal University School of Medicine, Changsha, Hunan, 410013, China; Department of Pharmacy, Hunan Normal University School of Medicine, Changsha, Hunan, 410013, China. ; Chao He:1.Department of General Surgery, The Second Xiangya Hospital, Central South University, Changsha, Hunan, 410011, China. Electronic address: chaohe@csu.edu.cn. ;
期刊：Ppic modulates CCl4-induced liver fibrosis and TGF-β-caused mouse hepatic stellate cell activation and regulated by miR-137-3p
DOI：10.1016/j.toxlet.2021.06.021
影响因子 ：3.5
引用产品： 小鼠肝星状细胞
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